ViewGene
Locus Name:
GeneID:
Location:

GeneID:2196
TrivialName: PA14_22050
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_22050
PAO1 Ortholog Locus:PA3242
Sequence Length:939
Protein Length:312
Start: 1916624
Stop: 1917562
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06630.1|AE004747_3 (AE004747) probable lauroyl acyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 310/312 (99%), Positives = 312/312 (100%)
Sequence:
ATGGATCGCCCCCGTTTCTCCAGCGCTTTCCTCCATCCCCGCTACTGGCCGCTCTGGTTCGGTCTGGGGCTG
CTCTGGCTGGTGGTGCAGTTGCCGTATCCCGTCCTGCTGATGCTCGGCCGTGGCCTGGGGGCGCTGATGTAC
CGCTTGGTCGGCTCGCGCCGCGAGATTGCCGCGCGCAACCTCGAACTGTGCTTTCCGGAAAAGTCGCCCGCC
GAACGCGAGCGTCTGCTGAAGGAGAACTTCGCCTCCAGCGGCATCGCCTTCTTCGAGATGGCCATGAGCTGG
TGGTGGCCGAAGGCGCGCCTGGCCCGTCTTGCCCATATAGAAGGCCTGGAGCATCTGCGCGAGGCCCAGGCC
AAGGGCGAGGGGGTGATCCTCATGGCCCTGCATTTCACCACCCTGGAGATCGGCGCGGCGCTGCTCGGCCAG
GTGCACACCATCGACGGCATGTACCGTGAGCACGACAACCCGGTGTTCGACTACATCCAGCGCCGTGGCCGC
GAGCGGCATAACCTCGATGCCACCGCCATCGAGCGCGAGGACGTGCGCTCGATGCTCAAGGTGCTGCGTGGC
GGCCGCGCCATCTGGTACGCACCGGACCAGGACTACGGCGCCAAGCAGAGCCTGTTCGTGCCGCTGTTCGGC
ATCCCGGCGGCCACCGTCACCGCCACTACCAAGTTCGCCCGCCTGGGCCGGGCGCGGGTGTTGCCGTTCACC
CAGAGCCGCCTGGCCGATGGCAGCGGTTATCGCCTGACCATCCACCCGCCGCTGGAGGACTTCCCCGGCGAG
AGCGAGGAAGCCGATTGCCTGCGGATCAACCAGTGGGTCGAGCGCTGCGTGCGCCAGCAGCCCGAGCAGTAC
CTGTGGGCGCACCGGCGTTTCAAGACCCGCCCGCCAGGCGAAGCCAAGCTCTATCCGAAACGGCGCAAGCGC
TGA
Translation:
MDRPRFSSAFLHPRYWPLWFGLGLLWLVVQLPYPVLLMLGRGLGALMYRLVGSRREIAARNLELCFPEKSPA
ERERLLKENFASSGIAFFEMAMSWWWPKARLARLAHIEGLEHLREAQAKGEGVILMALHFTTLEIGAALLGQ
VHTIDGMYREHDNPVFDYIQRRGRERHNLDATAIEREDVRSMLKVLRGGRAIWYAPDQDYGAKQSLFVPLFG
IPAATVTATTKFARLGRARVLPFTQSRLADGSGYRLTIHPPLEDFPGESEEADCLRINQWVERCVRQQPEQY
LWAHRRFKTRPPGEAKLYPKRRKR*
AnnotationID:2204GeneID:2196
AnnotatorUID: diggins
Modification Date/Time: 2005-04-14 14:02:02
Gene Name:htrB
Confidence Code:3
GeneProduct:putative lipid A biosynthesis lauroyl/myristoyl acyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:heat shock protein
Functional Category Confidence Code:5
COGs:pfam03279,COG1560
Secondary Functional Category(ies):chaperones and heat shock protein
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57338.1| (AE016870) lipid A biosynthesis lauroyl acyltransferase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 310

 Score =  529 bits (1363), Expect = e-150
 Identities = 246/310 (79%), Positives = 281/310 (90%)

>gb|AAC74138.1| (AE000206) heat shock protein [Escherichia coli K12]
          Length = 306

 Score =  304 bits (778), Expect = 5e-82
 Identities = 144/304 (47%), Positives = 197/304 (64%), Gaps = 3/304 (0%)








 
Structural Features:
pfam03279, Lip_A_acyltrans, Bacterial lipid A biosynthesis acyltransferase.
CD-Length = 295 residues, 100.0% aligned
Score =  280 bits (718), Expect = 1e-76
Identities = 123/297 (41%), Positives = 177/297 (59%), Gaps = 4/297 (1%)

COG1560, HtrB, Lauroyl/myristoyl acyltransferase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 308 residues, 100.0% aligned
Score =  358 bits (919), Expect = 7e-100
Identities = 162/308 (52%), Positives = 197/308 (63%), Gaps = 1/308 (0%)







   
Genomic Context:






   
Comment:


















    ReferenceID:4395




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21541




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32300




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2196_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2196
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3742
                           PA3242_tr
                           translation of PA3242
                           312
                           99.35
                           99.99
                           0
                           1655.0
     

     
     
115472
                           gb|AAG06630.1|AE004747_3
                           (AE004747) probable lauroyl acyltransferase [Pseudomonas aeruginosa PAO1]
                           312
                           99.35
                           99.99
                           0
                           1655.0
     

     
     
115473
                           gb|AAO57338.1|
                           (AE016870) lipid A biosynthesis lauroyl acyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           312
                           78.84
                           90.06
                           2
                           1363.0
     

     
     
115474
                           gb|AAN67355.1|AE016361_9
                           (AE016780) lipid A biosynthesis lauroyl acyltransferase [Pseudomonas putida KT2440]
                           312
                           78.84
                           87.17
                           1
                           1339.0
     

     
     
115484
                           gb|AAL20085.1|
                           (AE008750) lauroyl/myristoyl acyltransferase involved in lipid A biosynthesis [Salmonella typhimurium LT2]
                           314
                           47.45
                           62.10
                           10
                           772.0
     

     






      

          
GC ORFID: 45198How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3242Glimmer Score: 

          
Start: 3037307Stop: 3036369

          
Length: 939


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3242 translation of PA3242Homolog Bit Score 642.0

          
Other Homologs:  psyr_15may02_Scaffold16_revised_gene2643 (ORF: BLASTX 529.0), t_PA3242 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGATCGCCCCCGTTTCTCCAGCGCTTTCCTCCATCCCCGCTACTGGCCGCTCTGGTTCGGTCTGGGGCTG 
CTCTGGCTGGTGGTGCAGTTGCCGTATCCCGTCCTGCTGATGCTCGGCCGTGGCCTGGGGGCGCTGATGTAC 
CGCTTGGTCGGCTCGCGCCGCGAGATTGCCGCGCGCAACCTCGAACTGTGCTTTCCGGAAAAGTCGCCCGCC 
GAACGCGAGCGTCTGCTGAAGGAGAACTTCGCCTCCAGCGGCATCGCCTTCTTCGAGATGGCCATGAGCTGG 
TGGTGGCCGAAGGCGCGCCTGGCCCGTCTTGCCCATATAGAAGGCCTGGAGCATCTGCGCGAGGCCCAGGCC 
AAGGGCGAGGGGGTGATCCTCATGGCCCTGCATTTCACCACCCTGGAGATCGGCGCGGCGCTGCTCGGCCAG 
GTGCACACCATCGACGGCATGTACCGTGAGCACGACAACCCGGTGTTCGACTACATCCAGCGCCGTGGCCGC 
GAGCGGCATAACCTCGATGCCACCGCCATCGAGCGCGAGGACGTGCGCTCGATGCTCAAGGTGCTGCGTGGC 
GGCCGCGCCATCTGGTACGCACCGGACCAGGACTACGGCGCCAAGCAGAGCCTGTTCGTGCCGCTGTTCGGC 
ATCCCGGCGGCCACCGTCACCGCCACTACCAAGTTCGCCCGCCTGGGCCGGGCGCGGGTGTTGCCGTTCACC 
CAGAGCCGCCTGGCCGATGGCAGCGGTTATCGCCTGACCATCCACCCGCCGCTGGAGGACTTCCCCGGCGAG 
AGCGAGGAAGCCGATTGCCTGCGGATCAACCAGTGGGTCGAGCGCTGCGTGCGCCAGCAGCCCGAGCAGTAC 
CTGTGGGCGCACCGGCGTTTCAAGACCCGCCCGCCAGGCGAAGCCAAGCTCTATCCGAAACGGCGCAAGCGC 
TGA