ViewGene
Locus Name:
GeneID:
Location:

GeneID:2186
TrivialName: PA14_10620
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_10620
PAO1 Ortholog Locus:PA4124
Sequence Length:924
Protein Length:307
Start: 915251
Stop: 914328
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07511.1|AE004829_1 (AE004829) homoprotocatechuate 2,3-dioxygenase [Pseudomonas aeruginosa PAO1]
Identities = 307/307 (100%), Positives = 307/307 (100%)
Sequence:
ATGGGCAAAGTCGCTCTGGCTGCCAAGATCACCCACGTACCCTCGCTGTACCTGTCCGAGCTGCCCGGCCCG
CGCCACGGCTGCCGCCAGCCGGCGATCGACGGGCACCGCGAGATCGGCCGGCGCTGCCGCGAACTGGGGGTC
GACACCATCGTGGTGTTCGACACCCACTGGCTGGTCAACGCCGGCTACCACATCAACTGCGCGCCGCACTTC
GAGGGGCTCTACACCAGCAACGAGCTGCCGCACTTCATCGCCAACATGGAGTACGGCTTCCCCGGCAACCCG
GAGCTGGGCCGCATCCTCGCCGAAGGCTGCAACGCGCTCGGCGTGGAGACCCTGGCCCACGACGCCACTACC
CTCGGCCCGGAGTACGGCACCCTGGTACCGATGCGCTACATGAACCAGGACCGCCACTTCAAGGTGGTCTCG
GTCTCCGCCCTGTGCACCGTGCATTACCTGAACGACAGCGCGCGCCTGGGCTGGGCCATGCGCAAGGCGGTG
GAAGAGCACTACGACGGCACGGTGGCGTTCCTCGCCAGCGGCTCACTGTCCCACCGCTTCGCCCAGAACGGC
CAGGCGCCGGACTTCTCCGACCGCATCTGGAGCCCGTTCCTCGAGGTCCTCGACCATGAAGTCGTGCAGATG
TGGCAGGAAGGCCGCTGGGCGGAATTCTGCGGGATGCTCCCGGAGTACGCCTCCAAGGGCCATGGCGAAGGC
TTCATGCACGACACCGCGATGCTCCTCGGCGCCCTCGGCTGGTCGGCCTACGACGGCAAGGCGGAGGTGGTG
ACGCCCTACTTCGGCTCCTCCGGCACCGGGCAGATCAACGCGGTATTCCCGGTCACCGCCCAGGACGGCTCG
GCGATTCCCGCCGCCCAAGCCGGCAACCCGGCCGGCGCCAGTTGCGCCAGCCGCCTCTGA
Translation:
MGKVALAAKITHVPSLYLSELPGPRHGCRQPAIDGHREIGRRCRELGVDTIVVFDTHWLVNAGYHINCAPHF
EGLYTSNELPHFIANMEYGFPGNPELGRILAEGCNALGVETLAHDATTLGPEYGTLVPMRYMNQDRHFKVVS
VSALCTVHYLNDSARLGWAMRKAVEEHYDGTVAFLASGSLSHRFAQNGQAPDFSDRIWSPFLEVLDHEVVQM
WQEGRWAEFCGMLPEYASKGHGEGFMHDTAMLLGALGWSAYDGKAEVVTPYFGSSGTGQINAVFPVTAQDGS
AIPAAQAGNPAGASCASRL*
AnnotationID:2194GeneID:2186
AnnotatorUID: diggins
Modification Date/Time: 2005-03-30 13:01:09
Gene Name:hpcB
Confidence Code:2
GeneProduct:homoprotocatechuate 2,3-dioxygenase
Cell Localization:()
Synonyms:hpaD
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:homoprotocatechuate dyoxygenase
Functional Category Confidence Code:5
COGs:pfam02900
Secondary Functional Category(ies):
EC Number:1.13.11.15
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAE31237.1| (BX640439) homoprotocatechuate 2,3-dioxygenase [Bordetella
           bronchiseptica]
          Length = 289

 Score =  422 bits (1086), Expect = e-117
 Identities = 198/283 (69%), Positives = 227/283 (80%)

>gb|AAL28115.1|AF433158_1 (AF433158) homoprotocatechuate 2,3-dioxygenase [Pseudomonas sp.
           DJ-12]
          Length = 287

 Score =  403 bits (1036), Expect = e-112
 Identities = 188/283 (66%), Positives = 221/283 (78%)

>gb|AAO17180.1| (AF346500) HpaD [Photorhabdus luminescens]
          Length = 292

 Score =  395 bits (1015), Expect = e-109
 Identities = 190/282 (67%), Positives = 221/282 (78%), Gaps = 1/282 (0%)








 
Structural Features:
pfam02900, LigB, Catalytic LigB subunit of aromatic ring-opening dioxygenase.
CD-Length = 270 residues, 100.0% aligned
Score =  204 bits (520), Expect = 1e-53
Identities = 81/281 (28%), Positives = 112/281 (39%), Gaps = 16/281 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4375




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21521




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36859




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2186_cdd.html


    		




 
URL:

















    ReferenceID:36962




  
Author/Investigator(s):
        Prieto MA, Diaz E, Garcia JL.





  
Title:
        Molecular characterization of the 4-hydroxyphenylacetate catabolic pathway of Escherichia coli W: engineering a mobile aromatic degradative cluster.




  
PubMed:
        8550403


MedLine:
        




    
Source:
J Bacteriol. 1996 Jan;178(1):111-20.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8550403

















    ReferenceID:36968




  
Author/Investigator(s):
        Roper DI, Cooper RA.





  
Title:
        Subcloning and nucleotide sequence of the 3,4-dihydroxyphenylacetate (homoprotocatechuate) 2,3-dioxygenase gene from Escherichia coli C.




  
PubMed:
        2261999


MedLine:
        




    
Source:
FEBS Lett. 1990 Nov 26;275(1-2):53-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=91085577&dopt=Abstract

















    ReferenceID:36976




  
Author/Investigator(s):
        Jenkins JR, Cooper RA.





  
Title:
        Molecular cloning, expression, and analysis of the genes of the homoprotocatechuate catabolic pathway of Escherichia coli C.




  
PubMed:
        3053656


MedLine:
        




    
Source:
J Bacteriol. 1988 Nov;170(11):5317-24.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3053656









           
Homologs By Global Alignment 

           

       



     
Gene ID:2186
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3729
                           PA4124_tr
                           translation of PA4124
                           307
                           99.99
                           99.99
                           0
                           1661.0
     

     
     
114723
                           gb|AAG07511.1|AE004829_1
                           (AE004829) homoprotocatechuate 2,3-dioxygenase [Pseudomonas aeruginosa PAO1]
                           307
                           99.99
                           99.99
                           0
                           1661.0
     

     
     
114724
                           emb|CAE31237.1|
                           (BX640439) homoprotocatechuate 2,3-dioxygenase [Bordetella bronchiseptica] [Bordetella bronchiseptica RB50]
                           309
                           64.07
                           73.46
                           22
                           1079.0
     

     
     
114726
                           gb|AAO17180.1|
                           (AF346500) HpaD [Photorhabdus luminescens]
                           307
                           63.19
                           73.28
                           15
                           1015.0
     

     
     
114725
                           gb|AAL28115.1|AF433158_1
                           (AF433158) homoprotocatechuate 2,3-dioxygenase [Pseudomonas sp. DJ-12]
                           307
                           61.88
                           72.96
                           20
                           1036.0
     

     






      

          
GC ORFID: 45188How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4124Glimmer Score: 

          
Start: 4038686Stop: 4039609

          
Length: 924


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4124 translation of PA4124Homolog Bit Score 644.0

          
Other Homologs:  t_PA4124 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGGCAAAGTCGCTCTGGCTGCCAAGATCACCCACGTACCCTCGCTGTACCTGTCCGAGCTGCCCGGCCCG 
CGCCACGGCTGCCGCCAGCCGGCGATCGACGGGCACCGCGAGATCGGCCGGCGCTGCCGCGAACTGGGGGTC 
GACACCATCGTGGTGTTCGACACCCACTGGCTGGTCAACGCCGGCTACCACATCAACTGCGCGCCGCACTTC 
GAGGGGCTCTACACCAGCAACGAGCTGCCGCACTTCATCGCCAACATGGAGTACGGCTTCCCCGGCAACCCG 
GAGCTGGGCCGCATCCTCGCCGAAGGCTGCAACGCGCTCGGCGTGGAGACCCTGGCCCACGACGCCACTACC 
CTCGGCCCGGAGTACGGCACCCTGGTACCGATGCGCTACATGAACCAGGACCGCCACTTCAAGGTGGTCTCG 
GTCTCCGCCCTGTGCACCGTGCATTACCTGAACGACAGCGCGCGCCTGGGCTGGGCCATGCGCAAGGCGGTG 
GAAGAGCACTACGACGGCACGGTGGCGTTCCTCGCCAGCGGCTCACTGTCCCACCGCTTCGCCCAGAACGGC 
CAGGCGCCGGACTTCTCCGACCGCATCTGGAGCCCGTTCCTCGAGGTCCTCGACCATGAAGTCGTGCAGATG 
TGGCAGGAAGGCCGCTGGGCGGAATTCTGCGGGATGCTCCCGGAGTACGCCTCCAAGGGCCATGGCGAAGGC 
TTCATGCACGACACCGCGATGCTCCTCGGCGCCCTCGGCTGGTCGGCCTACGACGGCAAGGCGGAGGTGGTG 
ACGCCCTACTTCGGCTCCTCCGGCACCGGGCAGATCAACGCGGTATTCCCGGTCACCGCCCAGGACGGCTCG 
GCGATTCCCGCCGCCCAAGCCGGCAACCCGGCCGGCGCCAGTTGCGCCAGCCGCCTCTGA