ViewGene
Locus Name:
GeneID:
Location:

GeneID:2174
TrivialName: PA14_41870
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_41870
PAO1 Ortholog Locus:PA1754
Sequence Length:975
Protein Length:324
Start: 3740424
Stop: 3739450
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05143.1|AE004601_8 (AE004601) transcriptional regulator CysB [Pseudomonas aeruginosa PAO1]
Identities = 324/324 (100%), Positives = 324/324 (100%)
Sequence:
ATGAAGCTTCAGCAATTGCGCTATATCTGGGAGGTTGCGCACCACGACCTCAACGTCTCCGCCACGGCGCAG
AGCCTCTATACTTCGCAGCCGGGCATCAGCAAGCAGATCCGCCTGCTGGAGGACGAACTGGGCGTCGAGGTG
TTCGCTCGCAGCGGCAAGCACCTGACCCGGGTGACGCCGGCGGGGGAGCGGATCATCCATACCGCCGGCGAG
ATCCTGCGCAAGGTCGAGAGCATCAAGCAGATCGCCCAGGAATTCTCCAACGAGAAGAAGGGCACGCTGTCC
ATCGCCACTACCCACACCCAGGCGCGCTATGCGCTGCCCAACGTGATCAGCGGCTTCATCAAGCAGTACCCG
GACGTCTCCCTGCACATGCACCAGGGCACGCCGATGCAGATCGCCGAAATGGCGGCCGACGGCACCGTCGAC
TTCGCCATCGCCACCGAGGCGCTGGAGTTGTTCGGCGACCTGATCATGATGCCCTGCTACCGCTGGAATCGC
TGCGTCATCGTGCCCCACGGGCATCCGCTGACCAAGCTGCCGAAACTGACCCTGGAAGCCCTGGCCGAGCAG
CCGATCGTCACCTACGTGTTCGGCTTCACTGGCCGTTCCAAGCTCGACGAAGCCTTCAGCCAGCGCGGCCTG
GTGCCCAAGGTGGTATTCACCGCGGCCGATGCCGACGTGATCAAGACCTACGTGCGGTTGGGCCTGGGGGTC
GGCATCGTGGCGCACATGGCGGTCGATCCGAAGCTCGACAACGACCTGGTGATCCTCGACGCCAGCCACCTG
TTCGAGTCCAGCGTGACCAAGATCGGCTTCCGCCGCGGCACCTTCCTGCGCGGCTTCATGTGCGACTTCATC
GAGAAATTCGCTCCGCACCTGACCCGCGAGCTGCTGGCCAAGGCCGTGCAATGCCACAACAAGGCCGAGCTG
GACGAGTTGTTCGACGGCATCGAACTGCCGGTCTATTGA
Translation:
MKLQQLRYIWEVAHHDLNVSATAQSLYTSQPGISKQIRLLEDELGVEVFARSGKHLTRVTPAGERIIHTAGE
ILRKVESIKQIAQEFSNEKKGTLSIATTHTQARYALPNVISGFIKQYPDVSLHMHQGTPMQIAEMAADGTVD
FAIATEALELFGDLIMMPCYRWNRCVIVPHGHPLTKLPKLTLEALAEQPIVTYVFGFTGRSKLDEAFSQRGL
VPKVVFTAADADVIKTYVRLGLGVGIVAHMAVDPKLDNDLVILDASHLFESSVTKIGFRRGTFLRGFMCDFI
EKFAPHLTRELLAKAVQCHNKAELDELFDGIELPVY*
AnnotationID:2182GeneID:2174
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 12:12:36
Gene Name:cysB
Confidence Code:1
GeneProduct:transcriptional regulator CysB
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(25) Transcriptional regulators
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0583,pfam03466
Secondary Functional Category(ies):amino acid biosynthesis and metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67940.1|AE016426_5 (AE016782) transcriptional regulator CysB [Pseudomonas putida
           KT2440]
          Length = 324

 Score =  594 bits (1532), Expect = e-169
 Identities = 295/324 (91%), Positives = 312/324 (96%)

>gb|AAO55793.1| (AE016864) Cys regulon transcriptional activator [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 324

 Score =  584 bits (1505), Expect = e-166
 Identities = 291/324 (89%), Positives = 307/324 (94%)

>gb|AAF95055.1| (AE004266) cys regulon transcriptional activator [Vibrio cholerae]
          Length = 324

 Score =  443 bits (1139), Expect = e-124
 Identities = 213/323 (65%), Positives = 265/323 (82%)








 
Structural Features:
COG0583, LysR, Transcriptional regulator [Transcription]
CD-Length = 297 residues, 100.0% aligned
Score =  154 bits (390), Expect = 1e-38
Identities = 87/303 (28%), Positives = 139/303 (45%), Gaps = 8/303 (2%)

pfam03466, LysR_substrate, LysR substrate binding domain. 
CD-Length = 209 residues,  99.5% aligned
Score =  129 bits (325), Expect = 5e-31







   
Genomic Context:






   
Comment:


















    ReferenceID:4351




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21497




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37170




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2174_cdd.html


    		




 
URL:

















    ReferenceID:37833




  
Author/Investigator(s):
        Delic-Attree I, Toussaint B, Garin J, Vignais PM.





  
Title:
        Cloning, sequence and mutagenesis of the structural gene of Pseudomonas aeruginosa CysB, which can activate algD transcription.




  
PubMed:
        9218775


MedLine:
        




    
Source:
Mol Microbiol. 1997 Jun;24(6):1275-84.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9218775









           
Homologs By Global Alignment 

           

       



     
Gene ID:2174
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3717
                           PA1754_tr
                           translation of PA1754
                           324
                           99.99
                           99.99
                           0
                           1664.0
     

     
     
114537
                           gb|AAG05143.1|AE004601_8
                           (AE004601) transcriptional regulator CysB [Pseudomonas aeruginosa PAO1]
                           324
                           99.99
                           99.99
                           0
                           1664.0
     

     
     
114538
                           gb|AAB53742.1|
                           (U95379) CysB [Pseudomonas aeruginosa]
                           324
                           99.99
                           99.99
                           0
                           1664.0
     

     
     
114539
                           gb|AAN67940.1|AE016426_5
                           (AE016782) transcriptional regulator CysB [Pseudomonas putida KT2440]
                           324
                           91.04
                           96.29
                           0
                           1532.0
     

     
     
114540
                           gb|AAO55793.1|
                           (AE016864) Cys regulon transcriptional activator [Pseudomonas syringae pv. tomato str. DC3000]
                           324
                           89.81
                           94.75
                           0
                           1505.0
     

     






      

          
GC ORFID: 45176How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1754Glimmer Score: 

          
Start: 1213485Stop: 1214459

          
Length: 975


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1754 translation of PA1754Homolog Bit Score 645.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1058 (ORF: BLASTX 584.0), t_PA1754 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGCTTCAGCAATTGCGCTATATCTGGGAGGTTGCGCACCACGACCTCAACGTCTCCGCCACGGCGCAG 
AGCCTCTATACTTCGCAGCCGGGCATCAGCAAGCAGATCCGCCTGCTGGAGGACGAACTGGGCGTCGAGGTG 
TTCGCTCGCAGCGGCAAGCACCTGACCCGGGTGACGCCGGCGGGGGAGCGGATCATCCATACCGCCGGCGAG 
ATCCTGCGCAAGGTCGAGAGCATCAAGCAGATCGCCCAGGAATTCTCCAACGAGAAGAAGGGCACGCTGTCC 
ATCGCCACTACCCACACCCAGGCGCGCTATGCGCTGCCCAACGTGATCAGCGGCTTCATCAAGCAGTACCCG 
GACGTCTCCCTGCACATGCACCAGGGCACGCCGATGCAGATCGCCGAAATGGCGGCCGACGGCACCGTCGAC 
TTCGCCATCGCCACCGAGGCGCTGGAGTTGTTCGGCGACCTGATCATGATGCCCTGCTACCGCTGGAATCGC 
TGCGTCATCGTGCCCCACGGGCATCCGCTGACCAAGCTGCCGAAACTGACCCTGGAAGCCCTGGCCGAGCAG 
CCGATCGTCACCTACGTGTTCGGCTTCACTGGCCGTTCCAAGCTCGACGAAGCCTTCAGCCAGCGCGGCCTG 
GTGCCCAAGGTGGTATTCACCGCGGCCGATGCCGACGTGATCAAGACCTACGTGCGGTTGGGCCTGGGGGTC 
GGCATCGTGGCGCACATGGCGGTCGATCCGAAGCTCGACAACGACCTGGTGATCCTCGACGCCAGCCACCTG 
TTCGAGTCCAGCGTGACCAAGATCGGCTTCCGCCGCGGCACCTTCCTGCGCGGCTTCATGTGCGACTTCATC 
GAGAAATTCGCTCCGCACCTGACCCGCGAGCTGCTGGCCAAGGCCGTGCAATGCCACAACAAGGCCGAGCTG 
GACGAGTTGTTCGACGGCATCGAACTGCCGGTCTATTGA