ViewGene
Locus Name:
GeneID:
Location:

GeneID:2166
TrivialName: PA14_00100
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_00100
PAO1 Ortholog Locus:PA0009
Sequence Length:948
Protein Length:315
Start: 13435
Stop: 12488
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03399.1|AE004440_9 (AE004440) glycyl-tRNA synthetase alpha chain [Pseudomonas aeruginosa PAO1]
Identities = 315/315 (100%), Positives = 315/315 (100%)
Sequence:
GTGAGCCAGACTACGCCCGCCGTGCGCACCTTCCAAGACCTGATTCTCGCCCTGCAGAACTACTGGGCCGAG
CAGGGCTGCGTGGTGCTGCAGCCCTACGACATGGAAGTGGGCGCCGGCACCTTCCACACCGCCACCTTCCTC
CGCGCCATTGGCCCGGAGACTTGGAACGCCGCCTACGTCCAGCCGAGCCGCCGCCCCACCGACGGCCGCTAC
GGCGAGAACCCCAACCGCCTGCAGCACTACTACCAGTTTCAGGTGGTCCTGAAGCCGAACCCAGAGAACTTC
CAGGAGCTGTATCTCGGCTCGCTGAAAGCCATCGGCATCGACCCGCTGGTCCACGACATCCGCTTCGTCGAG
GACAACTGGGAATCGCCGACCCTCGGCGCCTGGGGCCTGGGTTGGGAAATCTGGCTGAACGGCATGGAAGTC
ACCCAGTTCACCTACTTCCAGCAGGTCGGCGGCATCGAGTGCTATCCGGTCACCGGCGAGATCACCTACGGC
CTGGAGCGCCTGGCCATGTACCTGCAGGGCGTGGATTCGGTCTACGACCTGGTCTGGACCGACGGCCCGTTC
GGCAAGGTCACCTATGGCGACGTGTTCCACCAGAACGAGGTGGAGCAATCCACCTTCAACTTCGAGCACGCC
AACGTGCCGAAGCTGTTCGAACTGTTCGACTTCTACGAAAGCGAAGCCAACCGCCTGATCGCGCTGGAGCTG
CCGTTGCCGACCTACGAGATGGTCCTCAAGGCTTCGCATACCTTCAACCTGCTGGATGCGCGGCGCGCCATC
TCGGTCACCGAGCGCCAGCGCTACATCCTTCGCGTGCGTACCCTGGCCCGCGCCGTGGCGCAGAGCTACCTG
CAGGCCCGCGCGCGCCTCGGCTTCCCCATGGCCACCCCCGAATTGCGTGACGAAGTACTGGCCAAGCTGAAG
GAGGCCGAATGA
Translation:
MSQTTPAVRTFQDLILALQNYWAEQGCVVLQPYDMEVGAGTFHTATFLRAIGPETWNAAYVQPSRRPTDGRY
GENPNRLQHYYQFQVVLKPNPENFQELYLGSLKAIGIDPLVHDIRFVEDNWESPTLGAWGLGWEIWLNGMEV
TQFTYFQQVGGIECYPVTGEITYGLERLAMYLQGVDSVYDLVWTDGPFGKVTYGDVFHQNEVEQSTFNFEHA
NVPKLFELFDFYESEANRLIALELPLPTYEMVLKASHTFNLLDARRAISVTERQRYILRVRTLARAVAQSYL
QARARLGFPMATPELRDEVLAKLKEAE*
AnnotationID:2174GeneID:2166
AnnotatorUID: diggins
Modification Date/Time: 2005-03-22 11:11:02
Gene Name:glyQ
Confidence Code:2
GeneProduct:glycyl-tRNA synthetase alpha chain
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0752,pfam02091,cd00733
Secondary Functional Category(ies):amino acid biosynthesis and metabolims
EC Number:6.1.1.14
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN65695.1|AE016196_6 (AE016774) glycyl-tRNA synthetase, tetrameric type, alpha subunit
           [Pseudomonas putida KT2440]
          Length = 315

 Score =  622 bits (1603), Expect = e-177
 Identities = 300/315 (95%), Positives = 306/315 (97%)

>gb|AAB18211.1| (U73324) glycyl-tRNA synthetase alpha subunit [Moraxella
           catarrhalis]
          Length = 318

 Score =  533 bits (1372), Expect = e-151
 Identities = 252/301 (83%), Positives = 272/301 (90%)

>gb|AAN82815.1|AE016768_233 (AE016768) Glycyl-tRNA synthetase alpha chain [Escherichia coli
           CFT073]
          Length = 303

 Score =  487 bits (1253), Expect = e-137
 Identities = 228/296 (77%), Positives = 259/296 (87%), Gaps = 2/296 (0%)






 
Structural Features:
COG0752, GlyQ, Glycyl-tRNA synthetase, alpha subunit 
[Translation, ribosomal structure and biogenesis] 
CD-Length = 298 residues, 100.0% aligned
Score =  568 bits (1465), Expect = 3e-163
Identities = 235/298 (78%), Positives = 261/298 (87%)

pfam02091, tRNA_synt_2e, Glycyl-tRNA synthetase alpha subunit.
CD-Length = 285 residues, 100.0% aligned
Score =  526 bits (1357), Expect = 1e-150
Identities = 232/288 (80%), Positives = 257/288 (89%), Gaps = 3/288 (1%)

cd00733, GlyRS_alpha_core, Class II Glycyl-tRNA synthetase (GlyRS) 
alpha subunit core catalytic domain.
CD-Length = 279 residues, 100.0% aligned
Score =  515 bits (1327), Expect = 4e-147







   
Genomic Context:






   
Comment:


















    ReferenceID:14521




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21481




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33375




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2166_cdd.html


    		




 
URL:

















    ReferenceID:35529




  
Author/Investigator(s):
        Wagar EA, Giese MJ, Yasin B, Pang M.





  
Title:
        The glycyl-tRNA synthetase of Chlamydia trachomatis.




  
PubMed:
        7665503


MedLine:
        




    
Source:
J Bacteriol. 1995 Sep;177(17):5179-85.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7665503

















    ReferenceID:35530




  
Author/Investigator(s):
        Keng T, Webster TA, Sauer RT, Schimmel P.





  
Title:
        Gene for Escherichia coli glycyl-tRNA synthetase has tandem subunit coding regions in the same reading frame.




  
PubMed:
        6290471


MedLine:
        




    
Source:
J Biol Chem. 1982 Nov 10;257(21):12503-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6290471









           
Homologs By Global Alignment 

           

       



     
Gene ID:2166
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3708
                           PA0009_tr
                           translation of PA0009
                           315
                           99.99
                           99.99
                           0
                           1670.0
     

     
     
114303
                           gb|AAG03399.1|AE004440_9
                           (AE004440) glycyl-tRNA synthetase alpha chain [Pseudomonas aeruginosa PAO1]
                           315
                           99.68
                           99.99
                           0
                           1667.0
     

     
     
114304
                           gb|AAN65695.1|AE016196_6
                           (AE016774) glycyl-tRNA synthetase, tetrameric type, alpha subunit [Pseudomonas putida KT2440]
                           315
                           94.92
                           97.14
                           0
                           1599.0
     

     
     
114305
                           gb|AAO53738.1|
                           (AE016856) glycyl-tRNA synthetase, alpha subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           315
                           91.74
                           96.19
                           0
                           1557.0
     

     
     
114306
                           gb|AAB18211.1|
                           (U73324) glycyl-tRNA synthetase alpha subunit [Moraxella catarrhalis]
                           320
                           79.06
                           85.62
                           7
                           1366.0
     

     






      

          
GC ORFID: 45168How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0009Glimmer Score: 

          
Start: 5814772Stop: 5813825

          
Length: 948


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0009 translation of PA0009Homolog Bit Score 647.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene4103 (ORF: BLASTX 604.0), t_PA0009 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGAGCCAGACTACGCCCGCCGTGCGCACCTTCCAAGACCTGATTCTCGCCCTGCAGAACTACTGGGCCGAG 
CAGGGCTGCGTGGTGCTGCAGCCCTACGACATGGAAGTGGGCGCCGGCACCTTCCACACCGCCACCTTCCTC 
CGCGCCATTGGCCCGGAGACTTGGAACGCCGCCTACGTCCAGCCGAGCCGCCGCCCCACCGACGGCCGCTAC 
GGCGAGAACCCCAACCGCCTGCAGCACTACTACCAGTTTCAGGTGGTCCTGAAGCCGAACCCAGAGAACTTC 
CAGGAGCTGTATCTCGGCTCGCTGAAAGCCATCGGCATCGACCCGCTGGTCCACGACATCCGCTTCGTCGAG 
GACAACTGGGAATCGCCGACCCTCGGCGCCTGGGGCCTGGGTTGGGAAATCTGGCTGAACGGCATGGAAGTC 
ACCCAGTTCACCTACTTCCAGCAGGTCGGCGGCATCGAGTGCTATCCGGTCACCGGCGAGATCACCTACGGC 
CTGGAGCGCCTGGCCATGTACCTGCAGGGCGTGGATTCGGTCTACGACCTGGTCTGGACCGACGGCCCGTTC 
GGCAAGGTCACCTATGGCGACGTGTTCCACCAGAACGAGGTGGAGCAATCCACCTTCAACTTCGAGCACGCC 
AACGTGCCGAAGCTGTTCGAACTGTTCGACTTCTACGAAAGCGAAGCCAACCGCCTGATCGCGCTGGAGCTG 
CCGTTGCCGACCTACGAGATGGTCCTCAAGGCTTCGCATACCTTCAACCTGCTGGATGCGCGGCGCGCCATC 
TCGGTCACCGAGCGCCAGCGCTACATCCTTCGCGTGCGTACCCTGGCCCGCGCCGTGGCGCAGAGCTACCTG 
CAGGCCCGCGCGCGCCTCGGCTTCCCCATGGCCACCCCCGAATTGCGTGACGAAGTACTGGCCAAGCTGAAG 
GAGGCCGAATGA