ViewGene
Locus Name:
GeneID:
Location:

GeneID:2154
TrivialName: PA14_46070
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_46070
PAO1 Ortholog Locus:PA1421
Sequence Length:960
Protein Length:319
Start: 4096997
Stop: 4097956
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04810.1|AE004571_9 (AE004571) guanidinobutyrase [Pseudomonas aeruginosa PAO1]
Identities = 317/319 (99%), Positives = 319/319 (100%)
Sequence:
GTGGACAAGAATCTCCACCAGCCCCTGGGCGGCAATGAAATGCCCCGCTTCGGCGGCATCGCCACCATGATG
CGCCTGCCCCATGTCCAGTCCCCCGCCGAACTCGACGCCCTCGATGCCGCCTTCGTCGGCGTGCCCCTCGAC
ATCGGCACCTCGCTGCGCTCCGGTACCCGCTTCGGCCCCCGCGAGATCCGCGCCGAATCGGTGATGATCCGT
CCCTACAACATGGCCACCGGCGCCGCCCCCTTCGACTCGCTGAACGTGGCGGACATCGGCGACGTCGCGATC
AACACCTTCAACCTGCTGGAAGCCGTGCGCATCATCGAGCAGGAATACGACCGCATCCTCGGTCACGGCATC
CTCCCGCTGACCCTCGGCGGCGACCACACCATCACCCTGCCGATCCTCCGCGCGATCAAGAAGAAGCACGGC
AAGGTCGGCCTGGTCCACATCGACGCCCACGCCGACGTCAACGACCACATGTTCGGCGAGAAGATCGCCCAC
GGCACCACCTTCCGCCGCGCGGTGGAAGAAGACCTGCTGGATTGCGACCGCGTGGTGCAGATCGGCCTGCGC
GCCCAGGGCTACACCGCCGAGGACTTCAACTGGAGCCGCAAGCAGGGCTTCCGCGTGGTGCAGGCCGAGGAG
TGCTGGCACAAGTCGCTGGAGCCGCTGATGGCCGAGGTCCGCGAGAAGGTCGGCGGCGGCCCCGTCTACCTG
AGCTTCGACATCGACGGCATCGACCCGGCCTGGGCGCCCGGCACCGGCACCCCGGAAATCGGCGGCCTGACC
ACCATCCAGGCGATGGAAATCATCCGCGGCTGCCAGGGCCTGGACCTGGTCGGCTGCGACCTGGTCGAAGTC
TCACCGCCCTACGACACCACCGGCAACACTTCGTTGCTGGGCGCCAACCTGCTCTATGAAATGCTCTGCGTC
CTCCCGGGCGTGGTGCGTCGCTGA
Translation:
MDKNLHQPLGGNEMPRFGGIATMMRLPHVQSPAELDALDAAFVGVPLDIGTSLRSGTRFGPREIRAESVMIR
PYNMATGAAPFDSLNVADIGDVAINTFNLLEAVRIIEQEYDRILGHGILPLTLGGDHTITLPILRAIKKKHG
KVGLVHIDAHADVNDHMFGEKIAHGTTFRRAVEEDLLDCDRVVQIGLRAQGYTAEDFNWSRKQGFRVVQAEE
CWHKSLEPLMAEVREKVGGGPVYLSFDIDGIDPAWAPGTGTPEIGGLTTIQAMEIIRGCQGLDLVGCDLVEV
SPPYDTTGNTSLLGANLLYEMLCVLPGVVRR*
AnnotationID:2162GeneID:2154
AnnotatorUID: diggins
Modification Date/Time: 2005-04-27 10:10:30
Gene Name:gbuA
Confidence Code:1
GeneProduct:guanidinobutyrase
Cell Localization:()
Synonyms:speB1
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:agmatinase
Functional Category Confidence Code:5
COGs:COG0010,pfam00491
Secondary Functional Category(ies):central intermediary metabolism
EC Number:3.5.3.7
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70097.1|AE016649_5 (AE016791) agmatinase, putative [Pseudomonas putida KT2440]
          Length = 320

 Score =  594 bits (1532), Expect = e-169
 Identities = 287/319 (89%), Positives = 305/319 (95%), Gaps = 3/319 (0%)

>dbj|BAB96819.1| (AB085822) guanidinobutyrase [Arthrobacter sp. KUJ8602]
          Length = 353

 Score =  266 bits (681), Expect = 9e-71
 Identities = 129/307 (42%), Positives = 199/307 (64%), Gaps = 4/307 (1%)






 
Structural Features:
COG0010, SpeB, Arginase/agmatinase/formimionoglutamate hydrolase, 
arginase family 
[Amino acid transport and metabolism]
CD-Length = 305 residues,  98.7% aligned
Score =  253 bits (648), Expect = 2e-68
Identities = 124/305 (40%), Positives = 180/305 (59%), Gaps = 10/305 (3%)

pfam00491, Arginase, Arginase family.
CD-Length = 295 residues,  97.6% aligned
Score =  169 bits (430), Expect = 3e-43
Identities = 96/293 (32%), Positives = 150/293 (51%), Gaps = 26/293 (8%)







   
Genomic Context:






   
Comment:


















    ReferenceID:14519




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21457




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37421




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2154_cdd.html


    		




 
URL:

















    ReferenceID:37995




  
Author/Investigator(s):
        Nakada Y, Itoh Y.





  
Title:
        Characterization and regulation of the gbuA gene, encoding guanidinobutyrase in the arginine dehydrogenase pathway of Pseudomonas aeruginosa PAO1.




  
PubMed:
        12029055


MedLine:
        




    
Source:
J Bacteriol. 2002 Jun;184(12):3377-84.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12029055









           
Homologs By Global Alignment 

           

       



     
Gene ID:2154
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3689
                           PA1421_tr
                           translation of PA1421
                           319
                           99.37
                           99.99
                           0
                           1671.0
     

     
     
113993
                           gb|AAG04810.1|AE004571_9
                           (AE004571) guanidinobutyrase [Pseudomonas aeruginosa PAO1]
                           319
                           99.05
                           99.99
                           0
                           1668.0
     

     
     
113994
                           gb|AAN70097.1|AE016649_5
                           (AE016791) agmatinase, putative [Pseudomonas putida KT2440]
                           323
                           89.16
                           94.42
                           7
                           1538.0
     

     
     
3690
                           PA0288_tr
                           translation of PA0288
                           322
                           48.13
                           67.08
                           7
                           793.0
     

     
     
113995
                           gb|AAG03677.1|AE004466_11
                           (AE004466) agmatinase [Pseudomonas aeruginosa PAO1]
                           322
                           48.13
                           67.08
                           7
                           793.0
     

     






      

          
GC ORFID: 45156How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1421Glimmer Score: 

          
Start: 856912Stop: 855953

          
Length: 960


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1421 translation of PA1421Homolog Bit Score 648.0

          
Other Homologs:  t_PA0288 (ORF: BLASTX 308.0), t_PA1421 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGGACAAGAATCTCCACCAGCCCCTGGGCGGCAATGAAATGCCCCGCTTCGGCGGCATCGCCACCATGATG 
CGCCTGCCCCATGTCCAGTCCCCCGCCGAACTCGACGCCCTCGATGCCGCCTTCGTCGGCGTGCCCCTCGAC 
ATCGGCACCTCGCTGCGCTCCGGTACCCGCTTCGGCCCCCGCGAGATCCGCGCCGAATCGGTGATGATCCGT 
CCCTACAACATGGCCACCGGCGCCGCCCCCTTCGACTCGCTGAACGTGGCGGACATCGGCGACGTCGCGATC 
AACACCTTCAACCTGCTGGAAGCCGTGCGCATCATCGAGCAGGAATACGACCGCATCCTCGGTCACGGCATC 
CTCCCGCTGACCCTCGGCGGCGACCACACCATCACCCTGCCGATCCTCCGCGCGATCAAGAAGAAGCACGGC 
AAGGTCGGCCTGGTCCACATCGACGCCCACGCCGACGTCAACGACCACATGTTCGGCGAGAAGATCGCCCAC 
GGCACCACCTTCCGCCGCGCGGTGGAAGAAGACCTGCTGGATTGCGACCGCGTGGTGCAGATCGGCCTGCGC 
GCCCAGGGCTACACCGCCGAGGACTTCAACTGGAGCCGCAAGCAGGGCTTCCGCGTGGTGCAGGCCGAGGAG 
TGCTGGCACAAGTCGCTGGAGCCGCTGATGGCCGAGGTCCGCGAGAAGGTCGGCGGCGGCCCCGTCTACCTG 
AGCTTCGACATCGACGGCATCGACCCGGCCTGGGCGCCCGGCACCGGCACCCCGGAAATCGGCGGCCTGACC 
ACCATCCAGGCGATGGAAATCATCCGCGGCTGCCAGGGCCTGGACCTGGTCGGCTGCGACCTGGTCGAAGTC 
TCACCGCCCTACGACACCACCGGCAACACTTCGTTGCTGGGCGCCAACCTGCTCTATGAAATGCTCTGCGTC 
CTCCCGGGCGTGGTGCGTCGCTGA