ViewGene
Locus Name:
GeneID:
Location:

GeneID:2146
TrivialName: PA14_25600
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_25600
PAO1 Ortholog Locus:PA2973
Sequence Length:981
Protein Length:326
Start: 2238658
Stop: 2239638
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06361.1|AE004723_5 (AE004723) probable peptidase [Pseudomonas aeruginosa PAO1]
Identities = 326/326 (100%), Positives = 326/326 (100%)
Sequence:
ATGTCTGACGAGTGGAAGTCGGAAACGCCGAAGGCGAATGACGACAAGAGCTGGAAGCTGCTGGAGAAGGCG
GTTCTCGCCGGGGTCCAGGAGCAGCGGCGGGCGCGGCGCTGGGGGATATTCTTCAAGTCGCTGACCTTCATC
TATCTCTTTGTCGTGCTGCTGGCCTTCAGTCCGTTCGGCAGCCTGGAGAAATCGGCCTCGCGCAGCGGTAGC
CACACGGCGCTGATTGAGGTGAAGGGTATGATCGCCGACGATGAGCCGGCCAGTGCCGACAATATCGTCACG
GCCTTGCGCGCGGCGTTCAAGGATGAGGGTACCAAGGGGATCGTCCTGCGGATCAACAGTCCGGGCGGCAGT
CCGGTGCAGTCGGGCTATATCTATGACGAGATTCGTCGCCTGCGCGGCGAGCACCCGAACGTCAAGGTCTAT
GCGGTGATCAGCGACCTGGGTGCCTCCGGCGCCTACTACATCGCCAGCGCCGCCGACCAGATCTACGCCGAC
AAGGCCAGTCTGGTCGGTTCCATCGGCGTGACGGCGGCCAGCTTCGGTTTCGTCGGCACCATGGAGAAGCTC
GGTGTCGAGCGACGCGTCTATACCTCCGGCGAGCACAAGTCGTTCCTCGATCCTTTCCAGCCGCAAAAGCCC
GAGGAGACTCAGTTctGGCAGCAGGTGCTGGATACTACCCACAAGCAGTTCATCGACAGCGTGAAAAAAGGG
CGCGGCGACCGGCTCAAGGTCGAGGGGCATCCGGAGCTGTTCTCGGGTCTGGTCTGGTCCGGCGAACAGGCA
TTGCAGCTCGGTTTGATCGACGGTCTGGGCAATGCCAGCTATGTGGCGCGCGAAGTGGTGAAGGAGAAGAAG
ATCGAGGATTACACCGTGCAGGAGTCGCCTTTCGATCGCTTCGCCAAGAAGTTCGGTGCCAGTGTGGCGGAG
CGCCTCGCGCTGTGGATGGGCTGGCAGGGCCCGGTATTGCGCTGA
Translation:
MSDEWKSETPKANDDKSWKLLEKAVLAGVQEQRRARRWGIFFKSLTFIYLFVVLLAFSPFGSLEKSASRSGS
HTALIEVKGMIADDEPASADNIVTALRAAFKDEGTKGIVLRINSPGGSPVQSGYIYDEIRRLRGEHPNVKVY
AVISDLGASGAYYIASAADQIYADKASLVGSIGVTAASFGFVGTMEKLGVERRVYTSGEHKSFLDPFQPQKP
EETQFWQQVLDTTHKQFIDSVKKGRGDRLKVEGHPELFSGLVWSGEQALQLGLIDGLGNASYVAREVVKEKK
IEDYTVQESPFDRFAKKFGASVAERLALWMGWQGPVLR*
AnnotationID:2154GeneID:2146
AnnotatorUID: diggins
Modification Date/Time: 2005-04-11 07:07:06
Gene Name:
Confidence Code:3
GeneProduct:putative peptidase
Cell Localization:()
Synonyms:sppA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:putative periplasmic serine protease
Functional Category Confidence Code:5
COGs:COG0616,pfam01343
Secondary Functional Category(ies):intracellular trafficking and secretion
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67526.1|AE016381_3 (AE016781) peptidase, putative [Pseudomonas putida KT2440]
          Length = 329

 Score =  500 bits (1287), Expect = e-141
 Identities = 243/329 (73%), Positives = 287/329 (87%), Gaps = 3/329 (0%)

>gb|AAO57306.1| (AE016869) signal peptide peptidase SppA, 36K type [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 332

 Score =  485 bits (1249), Expect = e-136
 Identities = 244/334 (73%), Positives = 279/334 (83%), Gaps = 10/334 (2%)

>emb|CAD14746.1| (AL646062) PUTATIVE PROTEASE TRANSMEMBRANE PROTEIN [Ralstonia
           solanacearum]
          Length = 378

 Score =  303 bits (776), Expect = 9e-82
 Identities = 156/308 (50%), Positives = 217/308 (70%), Gaps = 8/308 (2%)






 
Structural Features:
COG0616, SppA, Periplasmic serine proteases (ClpP class) 
[Posttranslational modification, protein turnover, chaperones / 
Intracellular trafficking and secretion]
CD-Length = 317 residues, 100.0% aligned
Score =  245 bits (627), Expect = 5e-66
Identities = 116/322 (36%), Positives = 165/322 (51%), Gaps = 16/322 (4%)

pfam01343, Peptidase_S49, Peptidase family S49.
CD-Length = 154 residues,  95.5% aligned
Score =  125 bits (316), Expect = 6e-30
Identities = 54/149 (36%), Positives = 86/149 (57%), Gaps = 2/149 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4295




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21441




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:38093




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2146_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2146
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3678
                           PA2973_tr
                           translation of PA2973
                           326
                           99.99
                           99.99
                           0
                           1674.0
     

     
     
113795
                           gb|AAG06361.1|AE004723_5
                           (AE004723) probable peptidase [Pseudomonas aeruginosa PAO1]
                           326
                           99.99
                           99.99
                           0
                           1674.0
     

     
     
113796
                           gb|AAN67526.1|AE016381_3
                           (AE016781) peptidase, putative [Pseudomonas putida KT2440]
                           329
                           73.86
                           87.23
                           3
                           1290.0
     

     
     
113797
                           gb|AAO57306.1|
                           (AE016869) signal peptide peptidase SppA, 36K type [Pseudomonas syringae pv. tomato str. DC3000]
                           334
                           73.05
                           83.53
                           10
                           1257.0
     

     
     
113798
                           dbj|BAC41612.1|
                           (AB088420) probable peptidase [Pseudomonas resinovorans]
                           352
                           54.26
                           70.17
                           29
                           940.0
     

     






      

          
GC ORFID: 45148How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2973Glimmer Score: 

          
Start: 2715267Stop: 2714287

          
Length: 981


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2973 translation of PA2973Homolog Bit Score 649.0

          
Other Homologs:  psyr_15may02_Scaffold13_revised_gene2157 (ORF: BLASTX 483.0), t_PA2973 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCTGACGAGTGGAAGTCGGAAACGCCGAAGGCGAATGACGACAAGAGCTGGAAGCTGCTGGAGAAGGCG 
GTTCTCGCCGGGGTCCAGGAGCAGCGGCGGGCGCGGCGCTGGGGGATATTCTTCAAGTCGCTGACCTTCATC 
TATCTCTTTGTCGTGCTGCTGGCCTTCAGTCCGTTCGGCAGCCTGGAGAAATCGGCCTCGCGCAGCGGTAGC 
CACACGGCGCTGATTGAGGTGAAGGGTATGATCGCCGACGATGAGCCGGCCAGTGCCGACAATATCGTCACG 
GCCTTGCGCGCGGCGTTCAAGGATGAGGGTACCAAGGGGATCGTCCTGCGGATCAACAGTCCGGGCGGCAGT 
CCGGTGCAGTCGGGCTATATCTATGACGAGATTCGTCGCCTGCGCGGCGAGCACCCGAACGTCAAGGTCTAT 
GCGGTGATCAGCGACCTGGGTGCCTCCGGCGCCTACTACATCGCCAGCGCCGCCGACCAGATCTACGCCGAC 
AAGGCCAGTCTGGTCGGTTCCATCGGCGTGACGGCGGCCAGCTTCGGTTTCGTCGGCACCATGGAGAAGCTC 
GGTGTCGAGCGACGCGTCTATACCTCCGGCGAGCACAAGTCGTTCCTCGATCCTTTCCAGCCGCAAAAGCCC 
GAGGAGACTCAGTTctGGCAGCAGGTGCTGGATACTACCCACAAGCAGTTCATCGACAGCGTGAAAAAAGGG 
CGCGGCGACCGGCTCAAGGTCGAGGGGCATCCGGAGCTGTTCTCGGGTCTGGTCTGGTCCGGCGAACAGGCA 
TTGCAGCTCGGTTTGATCGACGGTCTGGGCAATGCCAGCTATGTGGCGCGCGAAGTGGTGAAGGAGAAGAAG 
ATCGAGGATTACACCGTGCAGGAGTCGCCTTTCGATCGCTTCGCCAAGAAGTTCGGTGCCAGTGTGGCGGAG 
CGCCTCGCGCTGTGGATGGGCTGGCAGGGCCCGGTATTGCGCTGA