ViewGene
Locus Name:
GeneID:
Location:

GeneID:2144
TrivialName: PA14_03650
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_03650
PAO1 Ortholog Locus:PA0280
Sequence Length:990
Protein Length:329
Start: 329082
Stop: 328093
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03669.1|AE004466_3 (AE004466) sulfate transport protein CysA [Pseudomonas aeruginosa PAO1]
Identities = 329/329 (100%), Positives = 329/329 (100%)
Sequence:
ATGAGCATCGAAATCCGTAACGTCAGCAAGAACTTCAACGCCTTCAAGGCCCTGGACGACATCAACCTCGAC
ATCCAGAGCGGCGAGCTGGTGGCCCTGCTCGGCCCCTCCGGGTGCGGCAAGACCACCCTGCTGCGGATCATC
GCCGGGCTGGAAACCCCGGACGCCGGCAACATCGTGTTCCATGGCGAGGACGTCTCGCAGCACGACGTGCGC
GACCGCAACGTCGGCTTCGTCTTCCAGCACTACGCGCTGTTCCGCCACATGACGGTGTTCGACAACGTCGCC
TTCGGCCTGCGCATGAAGCCCAAGGGCGAGCGGCCGGGCGAGTCCGCGATCAAGGCCAAGGTCCACGAGCTG
CTCAACATGGTGCAGCTCGACTGGCTCGCCGACCGCTATCCAGAGCAACTCTCCGGCGGCCAGCGGCAGCGT
ATCGCCCTGGCCCGCGCGCTGGCGGTGGAGCCGAAGATCCTGCTCCTCGACGAACCCTTCGGCGCCCTCGAC
GCCAAGGTGCGCAAGGAGCTGCGCCGCTGGCTGGCGCGCCTGCACGAGGAGATCAACCTGACCTCGGTGTTC
GTCACCCACGACCAGGAAGAGGCGATGGAAGTGGCCGACCGCATCGTGGTGATGAACAAAGGCGTGATCGAG
CAGATCGGCTCGCCCGGCGAGGTCTACGAGAACCCGGCCAGCGATTTCGTCTACCACTTCCTCGGCGACTCC
AACCGCCTGCAACTGGGCAACGACCAGCACCTGCTGTTCCGCCCCCACGAAGTGTCGCTGTCGCGTTCCGCG
GTGGCCGAGCACCGCGCCGCCGAGGTCCGCGACATCCGTCCGCTGGGGGCGATCACCCGGGTAACGCTGAAG
GTCGACGGCCAGGACGAACTGATCGAAGCCGAAGTGGTGAAGGACCACGACAGCCTGGCCGGCCTGGCCCGC
GGCGAGACCCTCTACTTCAAGCCGAAGGCCTTCCAGCCGGTGGCCAATCTCTGA
Translation:
MSIEIRNVSKNFNAFKALDDINLDIQSGELVALLGPSGCGKTTLLRIIAGLETPDAGNIVFHGEDVSQHDVR
DRNVGFVFQHYALFRHMTVFDNVAFGLRMKPKGERPGESAIKAKVHELLNMVQLDWLADRYPEQLSGGQRQR
IALARALAVEPKILLLDEPFGALDAKVRKELRRWLARLHEEINLTSVFVTHDQEEAMEVADRIVVMNKGVIE
QIGSPGEVYENPASDFVYHFLGDSNRLQLGNDQHLLFRPHEVSLSRSAVAEHRAAEVRDIRPLGAITRVTLK
VDGQDELIEAEVVKDHDSLAGLARGETLYFKPKAFQPVANL*
AnnotationID:2152GeneID:2144
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 05:05:17
Gene Name:cysA
Confidence Code:2
GeneProduct:sulfate transport protein CysA
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:sulfate ABC transporter, ATP-binding protein
Functional Category Confidence Code:5
COGs:COG1118
Secondary Functional Category(ies):
EC Number:3.6.3.25
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO53856.1| (AE016857) sulfate ABC transporter, ATP-binding protein CysA
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 326

 Score =  581 bits (1497), Expect = e-165
 Identities = 288/324 (88%), Positives = 308/324 (95%)

>gb|AAN70733.1|AE016717_1 (AE016793) sulfate ABC transporter, ATP-binding protein
           [Pseudomonas putida KT2440]
          Length = 329

 Score =  572 bits (1475), Expect = e-163
 Identities = 286/329 (86%), Positives = 303/329 (92%)

>emb|CAD15049.1| (AL646064) PROBABLE SULFATE TRANSPORT ATP-BINDING ABC TRANSPORTER
           PROTEIN [Ralstonia solanacearum]
          Length = 372

 Score =  383 bits (983), Expect = e-106
 Identities = 203/357 (56%), Positives = 254/357 (71%), Gaps = 30/357 (8%)






 
Structural Features:
COG1118, CysA, ABC-type sulfate/molybdate transport systems, ATPase component 
[Inorganic ion transport and metabolism]
CD-Length = 345 residues,  99.7% aligned
Score =  414 bits (1065), Expect = 9e-117
Identities = 203/355 (57%), Positives = 243/355 (68%), Gaps = 38/355 (10%)








   
Genomic Context:






   
Comment:


















    ReferenceID:4291




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21437




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33631




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2144_cdd.html


    		




 
URL:

















    ReferenceID:35444




  
Author/Investigator(s):
        Laudenbach DE, Grossman AR.





  
Title:
        Characterization and mutagenesis of sulfur-regulated genes in a cyanobacterium: evidence for function in sulfate transport.




  
PubMed:
        1708375


MedLine:
        




    
Source:
J Bacteriol. 1991 May;173(9):2739-50.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1708375

















    ReferenceID:35445




  
Author/Investigator(s):
        Sirko A, Hryniewicz M, Hulanicka D, Bock A.





  
Title:
        Sulfate and thiosulfate transport in Escherichia coli K-12: nucleotide sequence and expression of the cysTWAM gene cluster.




  
PubMed:
        2188958


MedLine:
        




    
Source:
J Bacteriol. 1990 Jun;172(6):3351-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2188958









           
Homologs By Global Alignment 

           

       



     
Gene ID:2144
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3664
                           PA0280_tr
                           translation of PA0280
                           329
                           99.99
                           99.99
                           0
                           1678.0
     

     
     
112953
                           gb|AAG03669.1|AE004466_3
                           (AE004466) sulfate transport protein CysA [Pseudomonas aeruginosa PAO1]
                           329
                           99.99
                           99.99
                           0
                           1678.0
     

     
     
112954
                           gb|AAO53856.1|
                           (AE016857) sulfate ABC transporter, ATP-binding protein CysA [Pseudomonas syringae pv. tomato str. DC3000]
                           329
                           87.53
                           93.92
                           3
                           1497.0
     

     
     
112955
                           gb|AAN70733.1|AE016717_1
                           (AE016793) sulfate ABC transporter, ATP-binding protein [Pseudomonas putida KT2440]
                           329
                           86.93
                           92.09
                           0
                           1475.0
     

     
     
112956
                           gb|AAR34725.1|
                           (AE017211) sulfate ABC transporter, ATP-binding protein [Geobacter sulfurreducens PCA]
                           343
                           59.18
                           72.88
                           14
                           995.0
     

     






      

          
GC ORFID: 45146How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0280Glimmer Score: 

          
Start: 6130423Stop: 6129434

          
Length: 990


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0280 translation of PA0280Homolog Bit Score 650.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene4051 (ORF: BLASTX 582.0), psyr_15may02_Scaffold16_revised_gene2680 (ORF: BLASTX 233.0), t_PA0326 (ORF: BLASTX 229.0), t_PA3607 (ORF: BLASTX 227.0), psyr_15may02_Scaffold7_revised_gene6993 (ORF: BLASTX 227.0), t_PA3254 (ORF: BLASTX 226.0), t_PA0280 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCATCGAAATCCGTAACGTCAGCAAGAACTTCAACGCCTTCAAGGCCCTGGACGACATCAACCTCGAC 
ATCCAGAGCGGCGAGCTGGTGGCCCTGCTCGGCCCCTCCGGGTGCGGCAAGACCACCCTGCTGCGGATCATC 
GCCGGGCTGGAAACCCCGGACGCCGGCAACATCGTGTTCCATGGCGAGGACGTCTCGCAGCACGACGTGCGC 
GACCGCAACGTCGGCTTCGTCTTCCAGCACTACGCGCTGTTCCGCCACATGACGGTGTTCGACAACGTCGCC 
TTCGGCCTGCGCATGAAGCCCAAGGGCGAGCGGCCGGGCGAGTCCGCGATCAAGGCCAAGGTCCACGAGCTG 
CTCAACATGGTGCAGCTCGACTGGCTCGCCGACCGCTATCCAGAGCAACTCTCCGGCGGCCAGCGGCAGCGT 
ATCGCCCTGGCCCGCGCGCTGGCGGTGGAGCCGAAGATCCTGCTCCTCGACGAACCCTTCGGCGCCCTCGAC 
GCCAAGGTGCGCAAGGAGCTGCGCCGCTGGCTGGCGCGCCTGCACGAGGAGATCAACCTGACCTCGGTGTTC 
GTCACCCACGACCAGGAAGAGGCGATGGAAGTGGCCGACCGCATCGTGGTGATGAACAAAGGCGTGATCGAG 
CAGATCGGCTCGCCCGGCGAGGTCTACGAGAACCCGGCCAGCGATTTCGTCTACCACTTCCTCGGCGACTCC 
AACCGCCTGCAACTGGGCAACGACCAGCACCTGCTGTTCCGCCCCCACGAAGTGTCGCTGTCGCGTTCCGCG 
GTGGCCGAGCACCGCGCCGCCGAGGTCCGCGACATCCGTCCGCTGGGGGCGATCACCCGGGTAACGCTGAAG 
GTCGACGGCCAGGACGAACTGATCGAAGCCGAAGTGGTGAAGGACCACGACAGCCTGGCCGGCCTGGCCCGC 
GGCGAGACCCTCTACTTCAAGCCGAAGGCCTTCCAGCCGGTGGCCAATCTCTGA