ViewGene
Locus Name:
GeneID:
Location:

GeneID:2142
TrivialName: PA14_19910
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_19910
PAO1 Ortholog Locus:PA3416
Sequence Length:1002
Protein Length:333
Start: 1715757
Stop: 1716758
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06804.1|AE004762_10 (AE004762) probable pyruvate dehydrogenase E1 component, beta chain [Pseudomonas aeruginosa PAO1]
Identities = 331/333 (99%), Positives = 331/333 (99%)
Sequence:
ATGAATGAGCGCAACGGCGAAGCGAAACGCCTGGCCCTTCTGGAGGCGGTCAACCTGGCGTTGCACCGGGCC
ATGGCCGAGGACGAGGCGGTGGTGGTGCTGGGCGAAGACGTCGGGGTCAACGGCGGGGTGTTTCGCGCCACC
CTCGGCCTGCGCGAGCGCTTCGGCTTCAAGCGGGTGCTGGATACGCCGCTGGCGGAGAACATGATCGCCGGG
CTGTCCATCGGCATGGCCGCCCAGGGCCTGAAGCCGGTGATGGAGATCCAGTTCATGGGCTTCATCTACGCC
GCCATGGAGCAACTGGTGTCCCATGCCAGCCGCCTGCGCAATCGCACCCGCGGCCGCCTGGCCTGCCCGTTG
GTGCTGCGCACGCCGATGGGCGCGGGAATCCGCGCGCCGGAGCACCACAGCGAGGCGACCGAGGCGATGTTC
GCGCACATCCCCGGCGTCCGCGTGCTGGTGCCGTCGTCGCCGGCGCGGGCCTACGGCCTGCTCCTGGCGGCG
ATCGACGATCCCGATCCGGTGATCTTCCTCGAACCGACCCGGCTCTACCGGATGAACCCGCAGCCGCTGGCG
GACGACGCCCGGCGCCTGCCGCTGGACAGTTGCTTCACCCTCCGCGAAGGCGGCGACCTGACCCTGGTCAGC
TGGGGCGCCAGCGTCCACGAGACCCTGCAGGCCGCCGAGCGCCTGGCGCAGCGCGGCATCGAGGCCGAAGTG
ATCGACGTCGCCTGCCTCAAGCCGCTCGACCTCGACACCCTCGAAGCTTCGGTGCGCAAGACCGGTCGCTGC
GTGATCGTCCATGAGGCGCCGAAGAGCGGCGGCCTTGGCGGGGAAATCGCCGCGAGCCTCTACGAGCGCGTC
CTGTTCGATCTGCGGGCGCCGATCCAGCGGGTCGCCGCGGCCGATATCCCGCCGCCGCTGTACCGCCTGGAA
CCGCTGTACATGCCCGCCGTGGAGGACATCCTGGCGGCCTGCGACACCGTGCTCGGCTACGCCTGA
Translation:
MNERNGEAKRLALLEAVNLALHRAMAEDEAVVVLGEDVGVNGGVFRATLGLRERFGFKRVLDTPLAENMIAG
LSIGMAAQGLKPVMEIQFMGFIYAAMEQLVSHASRLRNRTRGRLACPLVLRTPMGAGIRAPEHHSEATEAMF
AHIPGVRVLVPSSPARAYGLLLAAIDDPDPVIFLEPTRLYRMNPQPLADDARRLPLDSCFTLREGGDLTLVS
WGASVHETLQAAERLAQRGIEAEVIDVACLKPLDLDTLEASVRKTGRCVIVHEAPKSGGLGGEIAASLYERV
LFDLRAPIQRVAAADIPPPLYRLEPLYMPAVEDILAACDTVLGYA*
AnnotationID:2150GeneID:2142
AnnotatorUID: diggins
Modification Date/Time: 2005-04-18 12:12:20
Gene Name:
Confidence Code:3
GeneProduct:putative pyruvate dehydrogenase E1 component, beta subunit
Cell Localization:()
Synonyms:pdhB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0022
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO90183.1| (AE016961) dehydrogenase, E1 component, beta subunit, putative
           [Coxiella burnetii RSA 493]
          Length = 326

 Score =  425 bits (1092), Expect = e-118
 Identities = 203/324 (62%), Positives = 263/324 (81%)

>gb|AAM39747.1| (AE012140) pyruvate dehydrogenase E1 beta subunit [Xanthomonas
           campestris pv. campestris str. ATCC 33913]
          Length = 327

 Score =  402 bits (1032), Expect = e-111
 Identities = 197/315 (62%), Positives = 245/315 (77%)

>gb|AAD34203.1|AF068743_2 (AF068743) pyruvate decarboxylase E1 beta subunit [Haloferax
           volcanii]
          Length = 327

 Score =  326 bits (836), Expect = 1e-88
 Identities = 162/312 (51%), Positives = 217/312 (69%)






 
Structural Features:
COG0022, AcoB, Pyruvate/2-oxoglutarate dehydrogenase complex, 
dehydrogenase (E1) component, eukaryotic type, beta subunit 
[Energy production and conversion]
CD-Length = 324 residues, 100.0% aligned
Score =  447 bits (1151), Expect = 1e-126
Identities = 171/324 (52%), Positives = 228/324 (70%), Gaps = 1/324 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4287




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21433




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32150




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2142_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2142
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3660
                           PA3416_tr
                           translation of PA3416
                           333
                           99.39
                           99.39
                           0
                           1677.0
     

     
     
112838
                           gb|AAG06804.1|AE004762_10
                           (AE004762) probable pyruvate dehydrogenase E1 component, beta chain [Pseudomonas aeruginosa PAO1]
                           333
                           99.39
                           99.39
                           0
                           1677.0
     

     
     
112840
                           emb|CAD15500.1|
                           (AL646066) PUTATIVE PYRUVATE DECARBOXYLASE E1 (BETA SUBUNIT) OXIDOREDUCTASE PROTEIN [Ralstonia solanacearum]
                           333
                           62.76
                           78.07
                           7
                           1072.0
     

     
     
112839
                           gb|AAO90183.1|
                           (AE016961) dehydrogenase, E1 component, beta subunit, putative [Coxiella burnetii RSA 493]
                           333
                           60.96
                           78.97
                           7
                           1090.0
     

     
     
112841
                           gb|AAM39747.1|
                           (AE012140) pyruvate dehydrogenase E1 beta subunit [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           333
                           59.15
                           74.17
                           6
                           1025.0
     

     






      

          
GC ORFID: 45144How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3416Glimmer Score: 

          
Start: 3238175Stop: 3237174

          
Length: 1002


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3416 translation of PA3416Homolog Bit Score 650.0

          
Other Homologs:  t_PA2248 (ORF: BLASTX 252.0), t_PA4151 (ORF: BLASTX 224.0), t_PA3416 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAATGAGCGCAACGGCGAAGCGAAACGCCTGGCCCTTCTGGAGGCGGTCAACCTGGCGTTGCACCGGGCC 
ATGGCCGAGGACGAGGCGGTGGTGGTGCTGGGCGAAGACGTCGGGGTCAACGGCGGGGTGTTTCGCGCCACC 
CTCGGCCTGCGCGAGCGCTTCGGCTTCAAGCGGGTGCTGGATACGCCGCTGGCGGAGAACATGATCGCCGGG 
CTGTCCATCGGCATGGCCGCCCAGGGCCTGAAGCCGGTGATGGAGATCCAGTTCATGGGCTTCATCTACGCC 
GCCATGGAGCAACTGGTGTCCCATGCCAGCCGCCTGCGCAATCGCACCCGCGGCCGCCTGGCCTGCCCGTTG 
GTGCTGCGCACGCCGATGGGCGCGGGAATCCGCGCGCCGGAGCACCACAGCGAGGCGACCGAGGCGATGTTC 
GCGCACATCCCCGGCGTCCGCGTGCTGGTGCCGTCGTCGCCGGCGCGGGCCTACGGCCTGCTCCTGGCGGCG 
ATCGACGATCCCGATCCGGTGATCTTCCTCGAACCGACCCGGCTCTACCGGATGAACCCGCAGCCGCTGGCG 
GACGACGCCCGGCGCCTGCCGCTGGACAGTTGCTTCACCCTCCGCGAAGGCGGCGACCTGACCCTGGTCAGC 
TGGGGCGCCAGCGTCCACGAGACCCTGCAGGCCGCCGAGCGCCTGGCGCAGCGCGGCATCGAGGCCGAAGTG 
ATCGACGTCGCCTGCCTCAAGCCGCTCGACCTCGACACCCTCGAAGCTTCGGTGCGCAAGACCGGTCGCTGC 
GTGATCGTCCATGAGGCGCCGAAGAGCGGCGGCCTTGGCGGGGAAATCGCCGCGAGCCTCTACGAGCGCGTC 
CTGTTCGATCTGCGGGCGCCGATCCAGCGGGTCGCCGCGGCCGATATCCCGCCGCCGCTGTACCGCCTGGAA 
CCGCTGTACATGCCCGCCGTGGAGGACATCCTGGCGGCCTGCGACACCGTGCTCGGCTACGCCTGA