ViewGene
Locus Name:
GeneID:
Location:

GeneID:2127
TrivialName: PA14_16480
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_16480
PAO1 Ortholog Locus:PA3703
Sequence Length:1008
Protein Length:335
Start: 1412043
Stop: 1413050
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07090.1|AE004789_10 (AE004789) probable methylesterase [Pseudomonas aeruginosa PAO1]
Identities = 335/335 (100%), Positives = 335/335 (100%)
Sequence:
TTGAGGATCGGAATCGTCAATGACATGCCGTTGGCCGTCGAGGCGCTGCGCCGCGCGCTGGCCTTCGAGCCG
CAGCACCAGATCGTCTGGGTCGCCAGCAACGGTGCCGAGGCGGTGACCCAGTGCGCCGCCGATACGCCCGAC
GTGGTGCTGATGGACCTGCTCATGCCGGTGATGGACGGCGTCGAGGCAACTCGTCGGATCATGGCGGAAAGT
CCTTGCGCGATCGTGATTGTTACCGTCGATATCGAGCAGAATGTGCACCGGGTTTTCGAAGCGATGGGATAT
GGGGCGCTCGATGCGGTGAACACGCCGGCGCTGGGCATCGGCAACCCGCAAACGGCGGCGGCGCCGTTGTTG
CGCAAGATTCAGAACGTCGGCTGGCTGATCGGCCAGCGCGATAACCGTGGCAAGGTCCAGGTGGTGCCGCCG
AAGGCCGGCGGGGCGCGCCAGCGTCTGGTGGCGATCGGCGCCTCGGCTGGCGGTCCGGCGTCGCTCGCGGTG
TTGCTCAAGCAACTGCCGGCCAGTTTCAATGCCGCGGTGGTGCTGGTGCAGCATGTCGACGAGGTATTCGCC
GCCGGCATGGCCGAATGGCTGGCCAGCGAATCGAAGCTGCCGGTGCGCCTGGCGCGCGATGGCGAGCCGCCG
ATACCGGGGCAGATCCTGCTGGCGGGGACCAACAATCACATCCGCCTGCTGCGCAACGGTTCGCTGGTGTAC
ACCGCCGAACCGCGTTCTTTCGTCTACCGGCCGTCCATCGACGTGTTTTTCGAGAGCGTGGCGAATTATTGG
CGTGGCGATGCCGTGGGCGTGCTGCTGACCGGCATGGGCCGCGATGGCGCCCAGGGCCTCAAGCAGATGCGC
GAGCGCGGCTTCCTCACCATCGCCCAGGACCAGGCCAGTTGCGCGGTCTACGGGATGCCGAAGGCCGCGGCG
GCGATCGATGCCGCGGTGCAGATCCTTTCCCTGGAAAAGATCGCCCCGCGCCTGGCGGAGGTATTCGATTAG
Translation:
MRIGIVNDMPLAVEALRRALAFEPQHQIVWVASNGAEAVTQCAADTPDVVLMDLLMPVMDGVEATRRIMAES
PCAIVIVTVDIEQNVHRVFEAMGYGALDAVNTPALGIGNPQTAAAPLLRKIQNVGWLIGQRDNRGKVQVVPP
KAGGARQRLVAIGASAGGPASLAVLLKQLPASFNAAVVLVQHVDEVFAAGMAEWLASESKLPVRLARDGEPP
IPGQILLAGTNNHIRLLRNGSLVYTAEPRSFVYRPSIDVFFESVANYWRGDAVGVLLTGMGRDGAQGLKQMR
ERGFLTIAQDQASCAVYGMPKAAAAIDAAVQILSLEKIAPRLAEVFD*
AnnotationID:2135GeneID:2127
AnnotatorUID: danlee
Modification Date/Time: 2006-02-02 14:02:16
Gene Name:wspF
Confidence Code:3
GeneProduct:putative methylesterase, CheB-like
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2201,pfam01339
Secondary Functional Category(ies):Chemotaxis
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO92338.1| (AY074937) WspF [Pseudomonas fluorescens]
          Length = 336

 Score =  506 bits (1302), Expect = e-142
 Identities = 253/334 (75%), Positives = 292/334 (87%)

>gb|AAN67115.1|AE016340_6 (AE016779) methylesterase, CheB-like [Pseudomonas putida KT2440]
          Length = 337

 Score =  494 bits (1273), Expect = e-139
 Identities = 252/335 (75%), Positives = 287/335 (85%), Gaps = 1/335 (0%)






 
Structural Features:
COG2201, CheB, Chemotaxis response regulator containing a 
CheY-like receiver domain and a methylesterase domain 
[Cell motility and secretion / Signal transduction mechanisms]
CD-Length = 350 residues,  98.0% aligned
Score =  316 bits (812), Expect = 4e-87
Identities = 135/344 (39%), Positives = 196/344 (56%), Gaps = 10/344 (2%)

pfam01339, CheB_methylest, CheB methylesterase.
CD-Length = 184 residues,  99.5% aligned
Score =  163 bits (413), Expect = 7e-41
Identities = 77/183 (42%), Positives = 114/183 (62%), Gaps = 3/183 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14509




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21403




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31847




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2127_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2127
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3637
                           PA3703_tr
                           translation of PA3703
                           335
                           99.99
                           99.99
                           0
                           1687.0
     

     
     
112015
                           gb|AAG07090.1|AE004789_10
                           (AE004789) probable methylesterase [Pseudomonas aeruginosa PAO1]
                           335
                           99.70
                           99.99
                           0
                           1685.0
     

     
     
112016
                           gb|AAO92338.1|
                           (AY074937) WspF [Pseudomonas fluorescens]
                           336
                           75.00
                           86.90
                           1
                           1297.0
     

     
     
112017
                           gb|AAN67115.1|AE016340_6
                           (AE016779) methylesterase, CheB-like [Pseudomonas putida KT2440]
                           337
                           74.48
                           85.16
                           2
                           1270.0
     

     
     
112018
                           gb|AAO55019.1|
                           (AE016861) protein-glutamate methylesterase CheB [Pseudomonas syringae pv. tomato str. DC3000]
                           336
                           72.32
                           83.63
                           1
                           1233.0
     

     






      

          
GC ORFID: 45129How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3703Glimmer Score: 

          
Start: 3541894Stop: 3540887

          
Length: 1008


          
Start Codon: TTGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA3703 translation of PA3703Homolog Bit Score 654.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4586 (ORF: BLASTX 495.0), t_PA3703 (ORF: GLIMMER)

          
GC ORF Sequence

           
TTGAGGATCGGAATCGTCAATGACATGCCGTTGGCCGTCGAGGCGCTGCGCCGCGCGCTGGCCTTCGAGCCG 
CAGCACCAGATCGTCTGGGTCGCCAGCAACGGTGCCGAGGCGGTGACCCAGTGCGCCGCCGATACGCCCGAC 
GTGGTGCTGATGGACCTGCTCATGCCGGTGATGGACGGCGTCGAGGCAACTCGTCGGATCATGGCGGAAAGT 
CCTTGCGCGATCGTGATTGTTACCGTCGATATCGAGCAGAATGTGCACCGGGTTTTCGAAGCGATGGGATAT 
GGGGCGCTCGATGCGGTGAACACGCCGGCGCTGGGCATCGGCAACCCGCAAACGGCGGCGGCGCCGTTGTTG 
CGCAAGATTCAGAACGTCGGCTGGCTGATCGGCCAGCGCGATAACCGTGGCAAGGTCCAGGTGGTGCCGCCG 
AAGGCCGGCGGGGCGCGCCAGCGTCTGGTGGCGATCGGCGCCTCGGCTGGCGGTCCGGCGTCGCTCGCGGTG 
TTGCTCAAGCAACTGCCGGCCAGTTTCAATGCCGCGGTGGTGCTGGTGCAGCATGTCGACGAGGTATTCGCC 
GCCGGCATGGCCGAATGGCTGGCCAGCGAATCGAAGCTGCCGGTGCGCCTGGCGCGCGATGGCGAGCCGCCG 
ATACCGGGGCAGATCCTGCTGGCGGGGACCAACAATCACATCCGCCTGCTGCGCAACGGTTCGCTGGTGTAC 
ACCGCCGAACCGCGTTCTTTCGTCTACCGGCCGTCCATCGACGTGTTTTTCGAGAGCGTGGCGAATTATTGG 
CGTGGCGATGCCGTGGGCGTGCTGCTGACCGGCATGGGCCGCGATGGCGCCCAGGGCCTCAAGCAGATGCGC 
GAGCGCGGCTTCCTCACCATCGCCCAGGACCAGGCCAGTTGCGCGGTCTACGGGATGCCGAAGGCCGCGGCG 
GCGATCGATGCCGCGGTGCAGATCCTTTCCCTGGAAAAGATCGCCCCGCGCCTGGCGGAGGTATTCGATTAG