ViewGene
Locus Name:
GeneID:
Location:

GeneID:2115
TrivialName: PA14_42880
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_42880
PAO1 Ortholog Locus:PA1671
Sequence Length:990
Protein Length:329
Start: 3811489
Stop: 3810500
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05060.1|AE004594_12 (AE004594) serine-threonine kinase Stk1 [Pseudomonas aeruginosa PAO1]
Identities = 326/329 (99%), Positives = 326/329 (99%)
Sequence:
ATGAACGAACCGCTGTCGTCGCTGGAGCTGCTGCCGGAGGAGGAGGGCGGCGCGAACCTGACCTATTTCGCC
TTCGCCGCCCCGACCGCCTCGGCGCCGGACCTGCCGGCGGTGTTGCCCGGTCCGCTGCCGGACGTTCTTTCC
GGGCGCTACCGTCTGGAGCGGATGCTCGGCGCCGGCGGCATGGGCACGGTATACCGTGCCCGGGACCTGCTC
CATGAGCAGTTCGGCGACCCGGCGCCGCTGGTAGCATTGAAGCTGCTCAACGAATCGGTCGCCGAGTCACCC
GACGCCAGCGCCTTGCTCTATAGCGAGTTCGCCCTGACCCGGCGCTTGCGCCATCCGAACGTCGTGCGTCTG
TTCACCTTCGACGTCGACACCGTCTGCCAGCGCGCCTACATCGTCATGGAACTCATGCCTGGCCTGCCGCTC
GATCGCCTGCTCTGCGAGCGTCCCGAAGGCCTGCCCTGGTCCGAGTTGAGCGCGATCGCCCGGCCGTTGCTG
GACGCCCTGGCCTATGTCCACGAACAAGGGGTGCTGCATGGCGACCTGAAGCCGAGCAACGTGATGCTCGGC
GAGGAGGGCGTGCGGCTCTTCGACTTCGGCCTCGGCCAGGCGCAGGCAGGCATCCTGGACGGCCTGCCGCAG
CTCAGCCGGGGCCGCATCGACGCCTGGACCCCGGGCTACGCCGCTCCCGAACTGCTCGAGGGTGCGCCGTTG
TCGCCGGCAGCCGACCTGTATGCGCTGGCCTGCGTCCTCTACGAACTGGCCGACGGTCGCCATCCGTTCCGC
CGCCTGCCCTCGAACCAGGCCCGGGAGCAGGGGCTGGAGCGCCAGCTGCGCGCGCCCGGACACCTGCCTCGG
CGCTGCTGGCCGGCGCTGCGTACCGCCCTGAGCCTCGACCCCGAACGGCGCTGCATCGGCGTCCGCGAATTG
CAGGAAGCGCTGGGCGCGCGGCGTTCCTGGCTGGCCGGCGTTCTTGCCCGTTGA
Translation:
MNEPLSSLELLPEEEGGANLTYFAFAAPTASAPDLPAVLPGPLPDVLSGRYRLERMLGAGGMGTVYRARDLL
HEQFGDPAPLVALKLLNESVAESPDASALLYSEFALTRRLRHPNVVRLFTFDVDTVCQRAYIVMELMPGLPL
DRLLCERPEGLPWSELSAIARPLLDALAYVHEQGVLHGDLKPSNVMLGEEGVRLFDFGLGQAQAGILDGLPQ
LSRGRIDAWTPGYAAPELLEGAPLSPAADLYALACVLYELADGRHPFRRLPSNQAREQGLERQLRAPGHLPR
RCWPALRTALSLDPERRCIGVRELQEALGARRSWLAGVLAR*
AnnotationID:2123GeneID:2115
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 07:07:21
Gene Name:stk1
Confidence Code:1
GeneProduct:serine-threonine kinase Stk1
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd00180,smart00220,pfam00069
Secondary Functional Category(ies):signal transduction mechanisms
EC Number:2.7.1.37
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58838.1| (AE016875) serine/threonine protein kinase, putative [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 331

 Score =  422 bits (1085), Expect = e-117
 Identities = 213/319 (66%), Positives = 254/319 (79%), Gaps = 6/319 (1%)






 
Structural Features:
cd00180, S_TKc, Serine/Threonine protein kinases, catalytic domain. 
CD-Length = 256 residues,  98.4% aligned
Score =  180 bits (458), Expect = 2e-46
Identities = 88/269 (32%), Positives = 128/269 (47%), Gaps = 21/269 (7%)

smart00220, S_TKc, Serine/Threonine protein kinases, catalytic domain
CD-Length = 256 residues,  97.7% aligned
Score =  171 bits (435), Expect = 1e-43
Identities = 88/271 (32%), Positives = 127/271 (46%), Gaps = 30/271 (11%)

pfam00069, Pkinase, Protein kinase domain.
CD-Length = 258 residues,  95.3% aligned
Score =  162 bits (411), Expect = 6e-41
Identities = 87/263 (33%), Positives = 124/263 (47%), Gaps = 25/263 (9%)







   
Genomic Context:






   
Comment:


















    ReferenceID:4233




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21379




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37178




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2115_cdd.html


    		




 
URL:

















    ReferenceID:37879




  
Author/Investigator(s):
        Mukhopadhyay S, Kapatral V, Xu W, Chakrabarty AM.





  
Title:
        Characterization of a Hank's type serine/threonine kinase and serine/threonine phosphoprotein phosphatase in Pseudomonas aeruginosa.




  
PubMed:
        10542161


MedLine:
        




    
Source:
J Bacteriol. 1999 Nov;181(21):6615-22.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10542161









           
Homologs By Global Alignment 

           

       



     
Gene ID:2115
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3623
                           PA1671_tr
                           translation of PA1671
                           329
                           99.08
                           99.08
                           0
                           1691.0
     

     
     
111734
                           gb|AAG05060.1|AE004594_12
                           (AE004594) serine-threonine kinase Stk1 [Pseudomonas aeruginosa PAO1]
                           329
                           99.08
                           99.08
                           0
                           1691.0
     

     
     
111735
                           gb|AAO58838.1|
                           (AE016875) serine/threonine protein kinase, putative [Pseudomonas syringae pv. tomato str. DC3000]
                           342
                           62.86
                           75.14
                           24
                           1085.0
     

     






      

          
GC ORFID: 45117How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1671Glimmer Score: 

          
Start: 1142420Stop: 1143409

          
Length: 990


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1671 translation of PA1671Homolog Bit Score 655.0

          
Other Homologs:  t_PA1671 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACGAACCGCTGTCGTCGCTGGAGCTGCTGCCGGAGGAGGAGGGCGGCGCGAACCTGACCTATTTCGCC 
TTCGCCGCCCCGACCGCCTCGGCGCCGGACCTGCCGGCGGTGTTGCCCGGTCCGCTGCCGGACGTTCTTTCC 
GGGCGCTACCGTCTGGAGCGGATGCTCGGCGCCGGCGGCATGGGCACGGTATACCGTGCCCGGGACCTGCTC 
CATGAGCAGTTCGGCGACCCGGCGCCGCTGGTAGCATTGAAGCTGCTCAACGAATCGGTCGCCGAGTCACCC 
GACGCCAGCGCCTTGCTCTATAGCGAGTTCGCCCTGACCCGGCGCTTGCGCCATCCGAACGTCGTGCGTCTG 
TTCACCTTCGACGTCGACACCGTCTGCCAGCGCGCCTACATCGTCATGGAACTCATGCCTGGCCTGCCGCTC 
GATCGCCTGCTCTGCGAGCGTCCCGAAGGCCTGCCCTGGTCCGAGTTGAGCGCGATCGCCCGGCCGTTGCTG 
GACGCCCTGGCCTATGTCCACGAACAAGGGGTGCTGCATGGCGACCTGAAGCCGAGCAACGTGATGCTCGGC 
GAGGAGGGCGTGCGGCTCTTCGACTTCGGCCTCGGCCAGGCGCAGGCAGGCATCCTGGACGGCCTGCCGCAG 
CTCAGCCGGGGCCGCATCGACGCCTGGACCCCGGGCTACGCCGCTCCCGAACTGCTCGAGGGTGCGCCGTTG 
TCGCCGGCAGCCGACCTGTATGCGCTGGCCTGCGTCCTCTACGAACTGGCCGACGGTCGCCATCCGTTCCGC 
CGCCTGCCCTCGAACCAGGCCCGGGAGCAGGGGCTGGAGCGCCAGCTGCGCGCGCCCGGACACCTGCCTCGG 
CGCTGCTGGCCGGCGCTGCGTACCGCCCTGAGCCTCGACCCCGAACGGCGCTGCATCGGCGTCCGCGAATTG 
CAGGAAGCGCTGGGCGCGCGGCGTTCCTGGCTGGCCGGCGTTCTTGCCCGTTGA