ViewGene
Locus Name:
GeneID:
Location:

GeneID:2112
TrivialName: PA14_10260
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_10260
PAO1 Ortholog Locus:PA4150
Sequence Length:975
Protein Length:324
Start: 884285
Stop: 883311
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07537.1|AE004831_9 (AE004831) probable dehydrogenase E1 component [Pseudomonas aeruginosa PAO1]
Identities = 322/324 (99%), Positives = 324/324 (100%)
Sequence:
ATGTCCACTCTGAGCACCGACCAACTGCTCCATGCCTACCGCGTGATGCGCACCATCCGCGCCTTCGAGGAA
CGCCTGCACGTCGAGTTCGCCACGGGCGAGATACCCGGCTTCGTCCACCTCTACGCCGGCGAGGAAGCCTCC
GCCGCCGGCGTGATGGCGCACCTGCGCAACGACGACTGCATCGCCTCCACCCATCGCGGCCACGGCCACTGC
ATCGCCAAGGGCGTAGACGTGCACGGCATGATGGCGGAGATCTATGGCAAGAAGACCGGGGTCTGCCAGGGC
AAGGGCGGCTCCATGCATATCGCCGACCTGGAGAAAGGCATGCTCGGCGCCAACGGAATCGTCGGCGCCGGC
GCGCCGCTGGCCGCCGGCGCCGCGCTGGCGGCCAAGCTGAAAGGCAGCGATGCGGTGGCGGTGGCCTTCTTC
GGCGATGGCGGCTCCAACGAAGGCGCGGTGTTCGAGGCGATGAACCTGGCCGCGGTGTGGAACCTGCCGTGC
CTGTTCGTCGCCGAGAACAACGGCTATGCCGAGGCCACCGCGGCCAACTGGTCGGTGGCCTGCGACCACATC
GCCGACCGCGCCGCCGGTTTCGGCATGCCCGGGGTGACCGTCGACGGCTTCGACTTCTTCGCCGTCCACGAG
GCTGCCGGCGCCGCCATCGAGCGCGCCCGCGCCGGCGAGGGGCCGTCGCTGATCGAAGTCAAGCTGACCCGC
TACTACGGACACTTCGAGGGCGACGCACAGACCTACCGCGATCCCGACGAAGTAAAGCACTACCGGGAAACC
CGCGACTGCCTCAAGCAGTTCCGCGAACGCACCTGCCACGCCGGCCTGCTCTCCGCCAGCGACCTGGACGCC
ATCGACGCCGAGGTCGAGGCGCGCATCGAGGATGCCGTGCAGCGCGCGAAGAACGATCCCAAGCCGGAGCCG
GACGACCTGCTGCGCGACGTCTACGTGTCCTATCCCTGA
Translation:
MSTLSTDQLLHAYRVMRTIRAFEERLHVEFATGEIPGFVHLYAGEEASAAGVMAHLRNDDCIASTHRGHGHC
IAKGVDVHGMMAEIYGKKTGVCQGKGGSMHIADLEKGMLGANGIVGAGAPLAAGAALAAKLKGSDAVAVAFF
GDGGSNEGAVFEAMNLAAVWNLPCLFVAENNGYAEATAANWSVACDHIADRAAGFGMPGVTVDGFDFFAVHE
AAGAAIERARAGEGPSLIEVKLTRYYGHFEGDAQTYRDPDEVKHYRETRDCLKQFRERTCHAGLLSASDLDA
IDAEVEARIEDAVQRAKNDPKPEPDDLLRDVYVSYP*
AnnotationID:2120GeneID:2112
AnnotatorUID: diggins
Modification Date/Time: 2005-03-30 16:04:52
Gene Name:
Confidence Code:3
GeneProduct:putative acetoin dehydrogenase E1 component
Cell Localization:()
Synonyms:acoA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:acetoin dehydrogenase, alpha subunit
Functional Category Confidence Code:5
COGs:COG1071,pfam00676
Secondary Functional Category(ies):energy production and conversion
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66182.1|AE016245_11 (AE016776) acetoin dehydrogenase, alpha subunit [Pseudomonas putida
           KT2440]
          Length = 325

 Score =  571 bits (1471), Expect = e-162
 Identities = 278/321 (86%), Positives = 300/321 (93%)

>gb|AAA21948.1| (M66060) acetoin:DCPIP oxidoreductase-alpha [Ralstonia eutropha]
          Length = 333

 Score =  426 bits (1095), Expect = e-119
 Identities = 209/320 (65%), Positives = 240/320 (75%)

>gb|AAA91875.1| (L24124) acetoin:DCPIP oxidoreductase alpha subunit [Pelobacter
           carbinolicus]
          Length = 326

 Score =  411 bits (1056), Expect = e-114
 Identities = 208/322 (64%), Positives = 249/322 (77%), Gaps = 2/322 (0%)






 
Structural Features:
COG1071, AcoA, Pyruvate/2-oxoglutarate dehydrogenase complex, 
dehydrogenase (E1) component, eukaryotic type, alpha subunit 
[Energy production and conversion]
CD-Length = 358 residues,  91.1% aligned
Score =  379 bits (975), Expect = 3e-106
Identities = 161/327 (49%), Positives = 214/327 (65%), Gaps = 4/327 (1%)

pfam00676, E1_dh, Dehydrogenase E1 component.
CD-Length = 302 residues,  99.3% aligned
Score =  322 bits (826), Expect = 4e-89
Identities = 138/300 (46%), Positives = 188/300 (62%), Gaps = 2/300 (0%)







   
Genomic Context:






   
Comment:


















    ReferenceID:4227




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21373




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36888




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2112_cdd.html


    		




 
URL:

















    ReferenceID:36981




  
Author/Investigator(s):
        Priefert H, Hein S, Kruger N, Zeh K, Schmidt B, Steinbuchel A.





  
Title:
        Identification and molecular characterization of the Alcaligenes eutrophus H16 aco operon genes involved in acetoin catabolism.




  
PubMed:
        2061286


MedLine:
        




    
Source:
J Bacteriol. 1991 Jul;173(13):4056-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2061286

















    ReferenceID:36986




  
Author/Investigator(s):
        Kruger N, Oppermann FB, Lorenzl H, Steinbuchel A.





  
Title:
        Biochemical and molecular characterization of the Clostridium magnum acetoin dehydrogenase enzyme system.




  
PubMed:
        8206840


MedLine:
        




    
Source:
J Bacteriol. 1994 Jun;176(12):3614-30.

Erratum in:
    * J Bacteriol 1994 Aug;176(16):5193.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8206840

















    ReferenceID:36988




  
Author/Investigator(s):
        Liao X, Charlebois I, Ouellet C, Morency MJ, Dewar K, Lightfoot J, Foster J, Siehnel R, Schweizer H, Lam JS, Hancock RE, Levesque RC.





  
Title:
        Physical mapping of 32 genetic markers on the Pseudomonas aeruginosa PAO1 chromosome.




  
PubMed:
        8581173


MedLine:
        




    
Source:
Microbiology. 1996 Jan;142 ( Pt 1):79-86.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8581173

















    ReferenceID:36989




  
Author/Investigator(s):
        Oppermann FB, Steinbuchel A.





  
Title:
        Identification and molecular characterization of the aco genes encoding the Pelobacter carbinolicus acetoin dehydrogenase enzyme system.




  
PubMed:
        8110297


MedLine:
        




    
Source:
J Bacteriol. 1994 Jan;176(2):469-85.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8110297

















    ReferenceID:36995




  
Author/Investigator(s):
        Huang M, Oppermann FB, Steinbuchel A.





  
Title:
        Molecular characterization of the Pseudomonas putida 2,3-butanediol catabolic pathway.




  
PubMed:
        7813883


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1994 Dec 1;124(2):141-50.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=95113288&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:2112
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3618
                           PA4150_tr
                           translation of PA4150
                           324
                           99.38
                           99.99
                           0
                           1693.0
     

     
     
111658
                           gb|AAG07537.1|AE004831_9
                           (AE004831) probable dehydrogenase E1 component [Pseudomonas aeruginosa PAO1]
                           324
                           99.38
                           99.99
                           0
                           1693.0
     

     
     
111659
                           gb|AAN66182.1|AE016245_11
                           (AE016776) acetoin dehydrogenase, alpha subunit [Pseudomonas putida KT2440]
                           325
                           85.53
                           92.61
                           1
                           1470.0
     

     
     
111660
                           gb|AAB58979.1|
                           (L35343) TPP-dependent acetoin dehydrogenase alpha-subunit [Pseudomonas putida]
                           325
                           84.92
                           92.61
                           1
                           1468.0
     

     
     
111663
                           gb|AAA18915.1|
                           (U01100) acetoin:DCPIP oxidoreductase alpha subunit [Pelobacter carbinolicus]
                           328
                           64.63
                           77.13
                           6
                           1062.0
     

     






      

          
GC ORFID: 45114How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4150Glimmer Score: 

          
Start: 4069652Stop: 4070626

          
Length: 975


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4150 translation of PA4150Homolog Bit Score 656.0

          
Other Homologs:  t_PA4150 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCACTCTGAGCACCGACCAACTGCTCCATGCCTACCGCGTGATGCGCACCATCCGCGCCTTCGAGGAA 
CGCCTGCACGTCGAGTTCGCCACGGGCGAGATACCCGGCTTCGTCCACCTCTACGCCGGCGAGGAAGCCTCC 
GCCGCCGGCGTGATGGCGCACCTGCGCAACGACGACTGCATCGCCTCCACCCATCGCGGCCACGGCCACTGC 
ATCGCCAAGGGCGTAGACGTGCACGGCATGATGGCGGAGATCTATGGCAAGAAGACCGGGGTCTGCCAGGGC 
AAGGGCGGCTCCATGCATATCGCCGACCTGGAGAAAGGCATGCTCGGCGCCAACGGAATCGTCGGCGCCGGC 
GCGCCGCTGGCCGCCGGCGCCGCGCTGGCGGCCAAGCTGAAAGGCAGCGATGCGGTGGCGGTGGCCTTCTTC 
GGCGATGGCGGCTCCAACGAAGGCGCGGTGTTCGAGGCGATGAACCTGGCCGCGGTGTGGAACCTGCCGTGC 
CTGTTCGTCGCCGAGAACAACGGCTATGCCGAGGCCACCGCGGCCAACTGGTCGGTGGCCTGCGACCACATC 
GCCGACCGCGCCGCCGGTTTCGGCATGCCCGGGGTGACCGTCGACGGCTTCGACTTCTTCGCCGTCCACGAG 
GCTGCCGGCGCCGCCATCGAGCGCGCCCGCGCCGGCGAGGGGCCGTCGCTGATCGAAGTCAAGCTGACCCGC 
TACTACGGACACTTCGAGGGCGACGCACAGACCTACCGCGATCCCGACGAAGTAAAGCACTACCGGGAAACC 
CGCGACTGCCTCAAGCAGTTCCGCGAACGCACCTGCCACGCCGGCCTGCTCTCCGCCAGCGACCTGGACGCC 
ATCGACGCCGAGGTCGAGGCGCGCATCGAGGATGCCGTGCAGCGCGCGAAGAACGATCCCAAGCCGGAGCCG 
GACGACCTGCTGCGCGACGTCTACGTGTCCTATCCCTGA