ViewGene
Locus Name:
GeneID:
Location:

GeneID:2095
TrivialName: PA14_43640
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_43640
PAO1 Ortholog Locus:PA1614
Sequence Length:1023
Protein Length:340
Start: 3884751
Stop: 3883729
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05003.1|AE004589_6 (AE004589) glycerol-3-phosphate dehydrogenase, biosynthetic [Pseudomonas aeruginosa PAO1]
Identities = 340/340 (100%), Positives = 340/340 (100%)
Sequence:
ATGACAGAGCAGCAACCGATTGCCGTGCTCGGCGGCGGCAGTTTCGGCACCGCCATCGCCAACCTGCTGGCC
GAGAACGGCCAGGCGGTCCGCCAGTGGATGCGCGACCCGGAACAGGCCGAGGCGATCCGCACACGACGCGAG
AACCCGCGCTACCTGAAAGGGGTCAAGGTGCATCCCGGAGTCGACCCGGTCACCGACCTGGAGCGCACCCTG
GCCGATTGCCAACTGATCTTCGTCGCCTTGCCCTCCAGCGCCCTGCGCAAGGTGCTGCAACCACACCAGGCG
GCATTGACGGACAAGCTGCTGGTGAGCCTGACCAAGGGCATCGAGGCGCACACCTTCAAGCTGATGAGTGAG
ATCCTCGAGGAAATCGCCCCGCAAGCCCGCATCGGGGTGATTTCCGGCCCGAACCTGGCGCGCGAGATCGCA
GAGCACGAGCTGACCGCCACGGTGGTCGCCAGCGAGGACGACGAGCTGTGCGCCCGCGTCCAGGCGGCCCTG
CACGGCCGCACCTTCCGCGTCTACGCCAGCCGCGACCGCTTCGGCGTGGAACTCGGCGGTGCGCTGAAGAAC
GTCTACGCGATCATGGCCGGCCTGGCCGCGGCGATGGACATGGGCGAGAACACCCGCAGCATGCTGATCACC
CGCGCGCTGGCGGAAATGACCCGGTTCGCCGTCAAGCTCGGTGCCAACCCGATGACCTTTCTCGGCCTCGCC
GGCGTCGGCGACCTGATCGTCACCTGTTCCTCGCCGAAGAGCCGCAACTACCAGGTCGGCCACGCCCTCGGC
GAGGGGCTCAGCCTGGAGGAGGCGGTATCGCGCATGGGCGAGACCGCGGAGGGTGTGAACACTCTCAAGGTA
CTCAAGGAAAAGTCCGACGAGATGCAGGTGTACATGCCGCTGGTCGCCGGTCTCCATGCGATCCTCTTCGAA
GGCCGCACCCTGGCGCAGGTCATCCAGCTGCTGATGCGCGGCGAGCCGAAGACCGACGTCGATTTCATTCCC
ACCACCGGCTTCTGA
Translation:
MTEQQPIAVLGGGSFGTAIANLLAENGQAVRQWMRDPEQAEAIRTRRENPRYLKGVKVHPGVDPVTDLERTL
ADCQLIFVALPSSALRKVLQPHQAALTDKLLVSLTKGIEAHTFKLMSEILEEIAPQARIGVISGPNLAREIA
EHELTATVVASEDDELCARVQAALHGRTFRVYASRDRFGVELGGALKNVYAIMAGLAAAMDMGENTRSMLIT
RALAEMTRFAVKLGANPMTFLGLAGVGDLIVTCSSPKSRNYQVGHALGEGLSLEEAVSRMGETAEGVNTLKV
LKEKSDEMQVYMPLVAGLHAILFEGRTLAQVIQLLMRGEPKTDVDFIPTTGF*
AnnotationID:2103GeneID:2095
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 07:07:06
Gene Name:gpsA
Confidence Code:2
GeneProduct:glycerol-3-phosphate dehydrogenase, biosynthetic
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0240,pfam01210
Secondary Functional Category(ies):fatty acid and phospholipid metabolism
EC Number:1.1.1.94
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55729.1| (AE016863) glycerol-3-phosphate dehydrogenase (NAD(P)+)
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 341

 Score =  550 bits (1418), Expect = e-156
 Identities = 282/340 (82%), Positives = 305/340 (89%)

>gb|AAN69750.1|AE016611_8 (AE016789) glycerol-3-phosphate dehydrogenase, biosynthetic
           [Pseudomonas putida KT2440]
          Length = 341

 Score =  533 bits (1374), Expect = e-151
 Identities = 273/340 (80%), Positives = 301/340 (88%)








 
Structural Features:
COG0240, GpsA, Glycerol-3-phosphate dehydrogenase 
[Energy production and conversion]
CD-Length = 329 residues, 100.0% aligned
Score =  394 bits (1013), Expect = 9e-111
Identities = 156/329 (47%), Positives = 214/329 (65%), Gaps = 1/329 (0%)

pfam01210, NAD_Gly3P_dh, NAD-dependent glycerol-3-phosphate dehydrogenase.
CD-Length = 326 residues,  99.4% aligned
Score =  298 bits (764), Expect = 7e-82
Identities = 140/324 (43%), Positives = 194/324 (59%), Gaps = 7/324 (2%)








   
Genomic Context:






   
Comment:


















    ReferenceID:4193




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21339




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37248




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2095_cdd.html


    		




 
URL:

















    ReferenceID:37900




  
Author/Investigator(s):
        Morbidoni HR, de Mendoza D, Cronan JE Jr.





  
Title:
        Synthesis of sn-glycerol 3-phosphate, a key precursor of membrane lipids, in Bacillus subtilis.




  
PubMed:
        7592341


MedLine:
        




    
Source:
J Bacteriol. 1995 Oct;177(20):5899-905.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7592341









           
Homologs By Global Alignment 

           

       



     
Gene ID:2095
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3598
                           PA1614_tr
                           translation of PA1614
                           340
                           99.99
                           99.99
                           0
                           1700.0
     

     
     
110530
                           gb|AAG05003.1|AE004589_6
                           (AE004589) glycerol-3-phosphate dehydrogenase, biosynthetic [Pseudomonas aeruginosa PAO1]
                           340
                           99.99
                           99.99
                           0
                           1700.0
     

     
     
110531
                           gb|AAO55729.1|
                           (AE016863) glycerol-3-phosphate dehydrogenase (NAD(P)+) [Pseudomonas syringae pv. tomato str. DC3000]
                           341
                           82.69
                           89.44
                           1
                           1418.0
     

     
     
110532
                           gb|AAN69750.1|AE016611_8
                           (AE016789) glycerol-3-phosphate dehydrogenase, biosynthetic [Pseudomonas putida KT2440]
                           341
                           80.05
                           88.26
                           1
                           1374.0
     

     
     
110537
                           gb|AAM24819.1|
                           (AE013117) Glycerol 3-phosphate dehydrogenase [Thermoanaerobacter tengcongensis]
                           343
                           40.81
                           60.93
                           16
                           631.0
     

     






      

          
GC ORFID: 45097How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1614Glimmer Score: 

          
Start: 1069158Stop: 1070180

          
Length: 1023


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1614 translation of PA1614Homolog Bit Score 659.0

          
Other Homologs:  t_PA1614 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACAGAGCAGCAACCGATTGCCGTGCTCGGCGGCGGCAGTTTCGGCACCGCCATCGCCAACCTGCTGGCC 
GAGAACGGCCAGGCGGTCCGCCAGTGGATGCGCGACCCGGAACAGGCCGAGGCGATCCGCACACGACGCGAG 
AACCCGCGCTACCTGAAAGGGGTCAAGGTGCATCCCGGAGTCGACCCGGTCACCGACCTGGAGCGCACCCTG 
GCCGATTGCCAACTGATCTTCGTCGCCTTGCCCTCCAGCGCCCTGCGCAAGGTGCTGCAACCACACCAGGCG 
GCATTGACGGACAAGCTGCTGGTGAGCCTGACCAAGGGCATCGAGGCGCACACCTTCAAGCTGATGAGTGAG 
ATCCTCGAGGAAATCGCCCCGCAAGCCCGCATCGGGGTGATTTCCGGCCCGAACCTGGCGCGCGAGATCGCA 
GAGCACGAGCTGACCGCCACGGTGGTCGCCAGCGAGGACGACGAGCTGTGCGCCCGCGTCCAGGCGGCCCTG 
CACGGCCGCACCTTCCGCGTCTACGCCAGCCGCGACCGCTTCGGCGTGGAACTCGGCGGTGCGCTGAAGAAC 
GTCTACGCGATCATGGCCGGCCTGGCCGCGGCGATGGACATGGGCGAGAACACCCGCAGCATGCTGATCACC 
CGCGCGCTGGCGGAAATGACCCGGTTCGCCGTCAAGCTCGGTGCCAACCCGATGACCTTTCTCGGCCTCGCC 
GGCGTCGGCGACCTGATCGTCACCTGTTCCTCGCCGAAGAGCCGCAACTACCAGGTCGGCCACGCCCTCGGC 
GAGGGGCTCAGCCTGGAGGAGGCGGTATCGCGCATGGGCGAGACCGCGGAGGGTGTGAACACTCTCAAGGTA 
CTCAAGGAAAAGTCCGACGAGATGCAGGTGTACATGCCGCTGGTCGCCGGTCTCCATGCGATCCTCTTCGAA 
GGCCGCACCCTGGCGCAGGTCATCCAGCTGCTGATGCGCGGCGAGCCGAAGACCGACGTCGATTTCATTCCC 
ACCACCGGCTTCTGA