ViewGene
Locus Name:
GeneID:
Location:

GeneID:2085
TrivialName: PA14_69240
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_69240
PAO1 Ortholog Locus:PA5243
Sequence Length:1014
Protein Length:337
Start: 6177840
Stop: 6176827
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08628.1|AE004936_11 (AE004936) delta-aminolevulinic acid dehydratase [Pseudomonas aeruginosa PAO1]
Identities = 337/337 (100%), Positives = 337/337 (100%)
Sequence:
GTGAGCTTCACTCCCGCCAACCGCGCCTATCCCTACACCCGCCTGCGGCGCAATCGCCGCGATGACTTCTCC
CGCCGCCTGGTCCGCGAGAATGTCCTCACCGTCGACGACCTGATCCTGCCGGTGTTCGTCCTCGATGGGGTC
AACCAGCGTGAAAGCATTCCGTCGATGCCCGGCGTCGAGCGCCTGTCCATCGACCAGTTGCTGATCGAGGCG
GAAGAGTGGGTGGCCCTGGGCATTCCGGCGCTGGCGCTGTTCCCGGTGACCCCGGTGGAGAAGAAGTCCCTG
GACGCCGCCGAGGCATACAACCCGGAAGGCATCGCCCAGCGCGCCACCCGCGCCCTGCGCGAGCGCTTTCCG
GAGCTGGGCATCATCACCGACGTGGCGCTCGACCCGTTCACCACCCATGGCCAGGACGGCATCCTGGATGAC
GATGGTTACGTCCTCAACGACGTCAGCATCGACGTGCTGGTACGGCAGGCGCTGTCCCACGCCGAGGCCGGC
GCCCAGGTGGTAGCGCCCTCGGACATGATGGACGGTCGCATCGGTGCGATCCGCGAGGCCCTGGAGTCTGCC
GGTCACACCAATGTGCGGATCATGGCCTACTCGGCCAAGTACGCCAGCGCCTACTACGGTCCGTTCCGCGAT
GCGGTCGGCTCGGCTTCGAACCTCGGCAAGGGCAACAAGGCCACCTACCAGATGGATCCGGCGAACAGCGAC
GAGGCACTTCACGAAGTGGCCGCCGACCTGGCCGAAGGCGCCGACATGGTGATGGTCAAGCCGGGCATGCCC
TACCTCGACATCGTGCGTCGGGTGAAGGATGAATTCCGCGCCCCGACCTTTGTCTACCAGGTCAGCGGCGAG
TATGCGATGCACATGGGCGCCATCCAGAACGGCTGGCTGGCCGAATCGGTGATCCTCGAATCCCTTACCGCC
TTCAAACGTGCCGGCGCCGATGGCATTCTGACCTACTTCGCCAAGCAGGCCGCAGAACAATTAAGACGGGGG
CGTTGA
Translation:
MSFTPANRAYPYTRLRRNRRDDFSRRLVRENVLTVDDLILPVFVLDGVNQRESIPSMPGVERLSIDQLLIEA
EEWVALGIPALALFPVTPVEKKSLDAAEAYNPEGIAQRATRALRERFPELGIITDVALDPFTTHGQDGILDD
DGYVLNDVSIDVLVRQALSHAEAGAQVVAPSDMMDGRIGAIREALESAGHTNVRIMAYSAKYASAYYGPFRD
AVGSASNLGKGNKATYQMDPANSDEALHEVAADLAEGADMVMVKPGMPYLDIVRRVKDEFRAPTFVYQVSGE
YAMHMGAIQNGWLAESVILESLTAFKRAGADGILTYFAKQAAEQLRRGR*
AnnotationID:2093GeneID:2085
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 14:02:13
Gene Name:hemB
Confidence Code:1
GeneProduct:delta-aminolevulinic acid dehydratase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:porphobilinogen synthase
Functional Category Confidence Code:5
COGs:pfam00490
Secondary Functional Category(ies):
EC Number:4.2.1.24
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAA62930.1| (X91820) 5-aminolevulinic acid dehydratase; porphobilinogen
           synthase [Pseudomonas aeruginosa]
          Length = 337

 Score =  662 bits (1707), Expect = 0.0
 Identities = 337/337 (100%), Positives = 337/337 (100%)

>gb|AAO58677.1| (AE016875) delta-aminolevulinic acid dehydratase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 336

 Score =  574 bits (1480), Expect = e-163
 Identities = 285/334 (85%), Positives = 312/334 (93%)

>gb|AAN57180.1|AE015852_10 (AE015852) delta-aminolevulinic acid dehydratase [Shewanella
           oneidensis MR-1]
          Length = 336

 Score =  485 bits (1248), Expect = e-136
 Identities = 240/330 (72%), Positives = 280/330 (84%), Gaps = 1/330 (0%)






 
Structural Features:
pfam00490, ALAD, Delta-aminolevulinic acid dehydratase.
CD-Length = 322 residues, 100.0% aligned
Score =  424 bits (1091), Expect = 8e-120
Identities = 181/325 (55%), Positives = 236/325 (72%), Gaps = 4/325 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14499




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21319




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34838




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2085_cdd.html


    		




 
URL:

















    ReferenceID:35744




  
Author/Investigator(s):
        Frankenberg N, Kittel T, Hungerer C, Romling U, Jahn D.





  
Title:
        Cloning, mapping and functional characterization of the hemB gene of Pseudomonas aeruginosa, which encodes a magnesium-dependent 5-aminolevulinic acid dehydratase.




  
PubMed:
        9529530


MedLine:
        




    
Source:
Mol Gen Genet. 1998 Feb;257(4):485-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9529530









           
Homologs By Global Alignment 

           

       



     
Gene ID:2085
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3586
                           PA5243_tr
                           translation of PA5243
                           337
                           99.99
                           99.99
                           0
                           1706.0
     

     
     
110150
                           gb|AAG08628.1|AE004936_11
                           (AE004936) delta-aminolevulinic acid dehydratase [Pseudomonas aeruginosa PAO1]
                           337
                           99.70
                           99.99
                           0
                           1703.0
     

     
     
110151
                           emb|CAA62930.1|
                           (X91820) 5-aminolevulinic acid dehydratase [Pseudomonas aeruginosa]
                           337
                           99.70
                           99.99
                           0
                           1703.0
     

     
     
110152
                           gb|AAO58677.1|
                           (AE016875) delta-aminolevulinic acid dehydratase [Pseudomonas syringae pv. tomato str. DC3000]
                           337
                           84.86
                           93.17
                           1
                           1476.0
     

     
     
110153
                           gb|AAN57180.1|AE015852_10
                           (AE015852) delta-aminolevulinic acid dehydratase [Shewanella oneidensis MR-1]
                           338
                           71.00
                           82.84
                           3
                           1245.0
     

     






      

          
GC ORFID: 45087How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5243Glimmer Score: 

          
Start: 5441527Stop: 5440514

          
Length: 1014


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5243 translation of PA5243Homolog Bit Score 661.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3205 (ORF: BLASTX 574.0), t_PA5243 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGAGCTTCACTCCCGCCAACCGCGCCTATCCCTACACCCGCCTGCGGCGCAATCGCCGCGATGACTTCTCC 
CGCCGCCTGGTCCGCGAGAATGTCCTCACCGTCGACGACCTGATCCTGCCGGTGTTCGTCCTCGATGGGGTC 
AACCAGCGTGAAAGCATTCCGTCGATGCCCGGCGTCGAGCGCCTGTCCATCGACCAGTTGCTGATCGAGGCG 
GAAGAGTGGGTGGCCCTGGGCATTCCGGCGCTGGCGCTGTTCCCGGTGACCCCGGTGGAGAAGAAGTCCCTG 
GACGCCGCCGAGGCATACAACCCGGAAGGCATCGCCCAGCGCGCCACCCGCGCCCTGCGCGAGCGCTTTCCG 
GAGCTGGGCATCATCACCGACGTGGCGCTCGACCCGTTCACCACCCATGGCCAGGACGGCATCCTGGATGAC 
GATGGTTACGTCCTCAACGACGTCAGCATCGACGTGCTGGTACGGCAGGCGCTGTCCCACGCCGAGGCCGGC 
GCCCAGGTGGTAGCGCCCTCGGACATGATGGACGGTCGCATCGGTGCGATCCGCGAGGCCCTGGAGTCTGCC 
GGTCACACCAATGTGCGGATCATGGCCTACTCGGCCAAGTACGCCAGCGCCTACTACGGTCCGTTCCGCGAT 
GCGGTCGGCTCGGCTTCGAACCTCGGCAAGGGCAACAAGGCCACCTACCAGATGGATCCGGCGAACAGCGAC 
GAGGCACTTCACGAAGTGGCCGCCGACCTGGCCGAAGGCGCCGACATGGTGATGGTCAAGCCGGGCATGCCC 
TACCTCGACATCGTGCGTCGGGTGAAGGATGAATTCCGCGCCCCGACCTTTGTCTACCAGGTCAGCGGCGAG 
TATGCGATGCACATGGGCGCCATCCAGAACGGCTGGCTGGCCGAATCGGTGATCCTCGAATCCCTTACCGCC 
TTCAAACGTGCCGGCGCCGATGGCATTCTGACCTACTTCGCCAAGCAGGCCGCAGAACAATTAAGACGGGGG 
CGTTGA