ViewGene
Locus Name:
GeneID:
Location:

GeneID:2081
TrivialName: PA14_62000
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62000
PAO1 Ortholog Locus:PA4687
Sequence Length:1008
Protein Length:335
Start: 5529629
Stop: 5530636
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08074.1|AE004882_5 (AE004882) ferric iron-binding periplasmic protein HitA [Pseudomonas aeruginosa PAO1]
Identities = 335/335 (100%), Positives = 335/335 (100%)
Sequence:
ATGCCTGTTCGACTTCTCAACCGTTTCCGCCACGCCGCCCTGGCGTGCCTGCTCGGTTCGCTTTCCCTCAAT
GCGGCGGCCGATCCCGTCACCCTTACCCTCTACAATGGCCAGCACGCCGCCACCGGTATCGCCATCGCCAAG
GCCTTCCAGGACAAGACCGGCATCCAGGTGAAGATCCGCAAGGGCGGCGATGGCCAGCTCGCCAGCCAGATC
ACCGAGGAAGGCGCGCGCTCGCCGGCCGACGTGCTCTACACCGAGGAATCGCCACCGTTGATCCGCCTGGCC
AGCGCCGGCCTGCTGGCCAAGCTGGAGCCAGAGACCCTGGCCCTGGTCGAGCCGGAGCACGCCGGCGGCAAC
GGCGACTGGATCGGCATCACCGCCCGCACCCGGGTACTGGCCTACAACCCGAAGAAGATCGACGAGAAGGAT
CTGCCGAAGAGCCTGATGGATCTTTCCGATCCGTCCTGGTCCGGCCGTTTCGGCTTCGTGCCCACCAGTGGC
GCCTTCCTCGAACAGGTCGCCGCAGTGATCAAGCTCAAGGGCCagGAGGAAGCCGAGGATTGGCTGACCGGC
CTGAaggccTtCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGaggtcGaC
ATGGCGCTGATCAACAACTACTACTGGtacacccTgaaaaaggaaaagggcgagtTGaactcgcgcctgcac
taCttcggcaACCaGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCaaGTCCAGCAAGCAC
CCCAGGGaaGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGAGgAaGgcCAGAAGGCGATCCTCAGCCAGTCC
GCCGAGTACCCGATGCGCaagGGGATGCAGGCCGATCCGGCGCTGAAGCCGTTCGCCGAACTGGACCCACCG
AAACTGACCCCGGCCGACCTGGGTGAGGCCAGCGAGGCCCTCAGCCTCGAACGCGACGTTGGCCTGAATTGA
Translation:
MPVRLLNRFRHAALACLLGSLSLNAAADPVTLTLYNGQHAATGIAIAKAFQDKTGIQVKIRKGGDGQLASQI
TEEGARSPADVLYTEESPPLIRLASAGLLAKLEPETLALVEPEHAGGNGDWIGITARTRVLAYNPKKIDEKD
LPKSLMDLSDPSWSGRFGFVPTSGAFLEQVAAVIKLKGQEEAEDWLTGLKAFGSIYTNNVTAMKAVENGEVD
MALINNYYWYTLKKEKGELNSRLHYFGNQDPGALVTVSGAAVLKSSKHPREAQQFVAFMLSEEGQKAILSQS
AEYPMRKGMQADPALKPFAELDPPKLTPADLGEASEALSLERDVGLN*
AnnotationID:2089GeneID:2081
AnnotatorUID: diggins
Modification Date/Time: 2005-03-08 08:08:04
Gene Name:hitA
Confidence Code:2
GeneProduct:ferric iron-binding periplasmic protein HitA
Cell Localization:()
Synonyms:fbp
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:periplasmic iron-binding protein
Functional Category Confidence Code:5
COGs:COG1840
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70449.1|AE016686_3 (AE016792) iron ABC transporter, periplasmic iron-binding protein,
           putative [Pseudomonas putida KT2440]
          Length = 337

 Score =  406 bits (1043), Expect = e-112
 Identities = 194/333 (58%), Positives = 251/333 (75%), Gaps = 1/333 (0%)

>dbj|BAA21842.1| (D89339) ferric iron binding protein [Actinobacillus
           actinomycetemcomitans]
          Length = 332

 Score =  293 bits (749), Expect = 1e-78
 Identities = 156/333 (46%), Positives = 217/333 (65%), Gaps = 8/333 (2%)

>emb|CAB84125.1| (AL162754) major ferric iron binding protein [Neisseria
           meningitidis Z2491]
          Length = 331

 Score =  281 bits (720), Expect = 3e-75
 Identities = 148/329 (44%), Positives = 213/329 (64%), Gaps = 8/329 (2%)






 
Structural Features:
COG1840, AfuA, ABC-type Fe3+ transport system, periplasmic component 
[Inorganic ion transport and metabolism]
CD-Length = 299 residues, 100.0% aligned
Score =  168 bits (427), Expect = 6e-43
Identities = 89/304 (29%), Positives = 133/304 (43%), Gaps = 19/304 (6%)








   
Genomic Context:






   
Comment:


















    ReferenceID:4165




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21311




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37599




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2081_cdd.html


    		




 
URL:

















    ReferenceID:37758




  
Author/Investigator(s):
        Farinha MA, Ronald SL, Kropinski AM, Paranchych W.





  
Title:
        Localization of the virulence-associated genes pilA, pilR, rpoN, fliA, fliC, ent, and fbp on the physical map of Pseudomonas aeruginosa PAO1 by pulsed-field electrophoresis.




  
PubMed:
        8454366


MedLine:
        




    
Source:
Infect Immun. 1993 Apr;61(4):1571-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8454366

















    ReferenceID:37759




  
Author/Investigator(s):
        Berish SA, Kapczynski DR, Morse SA.





  
Title:
        Nucleotide sequence of the Fbp gene from Neisseria meningitidis.




  
PubMed:
        2117747


MedLine:
        




    
Source:
Nucleic Acids Res. 1990 Aug 11;18(15):4596.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2117747

















    ReferenceID:37760




  
Author/Investigator(s):
        Sedivy JM, Daldal F, Fraenkel DG.





  
Title:
        Fructose bisphosphatase of Escherichia coli: cloning of the structural gene (fbp) and preparation of a chromosomal deletion.




  
PubMed:
        6327623


MedLine:
        




    
Source:
J Bacteriol. 1984 Jun;158(3):1048-53.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6327623

















    ReferenceID:38231




  
Author/Investigator(s):
        Adhikari P, Kirby SD, Nowalk AJ, Veraldi KL, Schryvers AB, Mietzner TA.





  
Title:
        Biochemical characterization of a Haemophilus influenzae periplasmic iron transport operon.




  
PubMed:
        7559648


MedLine:
        




    
Source:
J Biol Chem. 1995 Oct 20;270(42):25142-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7559648&query_hl=10&itool=pubmed_docsum

















    ReferenceID:38235




  
Author/Investigator(s):
        Sanders JD, Cope LD, Hansen EJ.





  
Title:
        Identification of a locus involved in the utilization of iron by Haemophilus influenzae.




  
PubMed:
        


MedLine:
        




    
Source:
Infect Immun. 1994 Oct;62(10):4515-25.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=7927717









           
Homologs By Global Alignment 

           

       



     
Gene ID:2081
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3582
                           PA4687_tr
                           translation of PA4687
                           335
                           99.99
                           99.99
                           0
                           1707.0
     

     
     
110088
                           gb|AAG08074.1|AE004882_5
                           (AE004882) ferric iron-binding periplasmic protein HitA [Pseudomonas aeruginosa PAO1]
                           335
                           99.99
                           99.99
                           0
                           1707.0
     

     
     
110089
                           gb|AAN70449.1|AE016686_3
                           (AE016792) iron ABC transporter, periplasmic iron-binding protein, putative [Pseudomonas putida KT2440]
                           338
                           57.39
                           74.26
                           4
                           1041.0
     

     
     
110090
                           dbj|BAA21842.1|
                           (D89339) ferric iron binding protein [Actinobacillus actinomycetemcomitans]
                           341
                           46.04
                           64.22
                           15
                           758.0
     

     
     
110092
                           gb|AAB66501.1|
                           (U66056) periplasmic iron repressible protein [Actinobacillus actinomycetemcomitans]
                           341
                           45.45
                           63.63
                           15
                           735.0
     

     






      

          
GC ORFID: 45083How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4687Glimmer Score: 

          
Start: 4793309Stop: 4794316

          
Length: 1008


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4687 translation of PA4687Homolog Bit Score 662.0

          
Other Homologs:  t_PA4687 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCTGTTCGACTTCTCAACCGTTTCCGCCACGCCGCCCTGGCGTGCCTGCTCGGTTCGCTTTCCCTCAAT 
GCGGCGGCCGATCCCGTCACCCTTACCCTCTACAATGGCCAGCACGCCGCCACCGGTATCGCCATCGCCAAG 
GCCTTCCAGGACAAGACCGGCATCCAGGTGAAGATCCGCAAGGGCGGCGATGGCCAGCTCGCCAGCCAGATC 
ACCGAGGAAGGCGCGCGCTCGCCGGCCGACGTGCTCTACACCGAGGAATCGCCACCGTTGATCCGCCTGGCC 
AGCGCCGGCCTGCTGGCCAAGCTGGAGCCAGAGACCCTGGCCCTGGTCGAGCCGGAGCACGCCGGCGGCAAC 
GGCGACTGGATCGGCATCACCGCCCGCACCCGGGTACTGGCCTACAACCCGAAGAAGATCGACGAGAAGGAT 
CTGCCGAAGAGCCTGATGGATCTTTCCGATCCGTCCTGGTCCGGCCGTTTCGGCTTCGTGCCCACCAGTGGC 
GCCTTCCTCGAACAGGTCGCCGCAGTGATCAAGCTCAAGGGCCagGAGGAAGCCGAGGATTGGCTGACCGGC 
CTGAaggccTtCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGaggtcGaC 
ATGGCGCTGATCAACAACTACTACTGGtacacccTgaaaaaggaaaagggcgagtTGaactcgcgcctgcac 
taCttcggcaACCaGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCaaGTCCAGCAAGCAC 
CCCAGGGaaGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGAGgAaGgcCAGAAGGCGATCCTCAGCCAGTCC 
GCCGAGTACCCGATGCGCaagGGGATGCAGGCCGATCCGGCGCTGAAGCCGTTCGCCGAACTGGACCCACCG 
AAACTGACCCCGGCCGACCTGGGTGAGGCCAGCGAGGCCCTCAGCCTCGAACGCGACGTTGGCCTGAATTGA