ViewGene
Locus Name:
GeneID:
Location:

GeneID:2080
TrivialName: PA14_58150
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_58150
PAO1 Ortholog Locus:PA4481
Sequence Length:1038
Protein Length:345
Start: 5177022
Stop: 5175985
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07869.1|AE004862_3 (AE004862) rod shape-determining protein MreB [Pseudomonas aeruginosa PAO1]
Identities = 345/345 (100%), Positives = 345/345 (100%)
Sequence:
ATGTTCAAAAAATTGCGTGGCATGTTTTCCAGCGATCTGTCGATCGACCTGGGTACTGCCAATACCCTTATT
TATGTGCGCGAGCGCGGCATCGTCCTGAACGAACCGTCCGTGGTCGCCATTCGCAGCCATGGCAGCCAGAAG
AGCGTCGTCGCCGTGGGCACCGAGGCCAAGCGCATGCTCGGCCGTACCCCGGGCAACATCGCCGCCATCCGT
CCGATGAAAGACGGCGTGATCGCGGATTTCAGCGTCTGCGAGAAGATGCTGCAGTACTTCATCAACAAGGTT
CACGAGAACAGCTTCCTGCAGCCCAGCCCGCGCGTGCTGATCTGCGTGCCGTGCAAGTCCACCCAGGTTGAG
CGCCGGGCCATCCGCGAATCCGCCCTGGGCGCCGGCGCCCGCGAGGTGTTCCTGATCGAAGAGCCGATGGCT
GCTGCCATCGGCGCCGGCCTGCCGGTCGAGGAGGCCCGCGGCTCGATGGTCGTAGACATCGGCGGCGGCACT
ACCGAGATCGCCCTGATTTCCCTCAACGGCGTGGTCTACGCCGAATCCGTCCGCGTCGGCGGCGACCGCTTC
GACGAAGCCATCGTCACCTACGTGCGCCGCAACTACGGCAGCCTGATCGGCGAATCCACCGCCGAGCGCATC
AAGCAGGAGATCGGCACCGCCTTCCCGGGCGGCGACGTTCGCGAAGTCGACGTCCGCGGCCGTAACCTGGCC
GAAGGCGTACCGCGCAGCTTCACCCTGAACTCCAACGAGGTCCTCGAGGCCCTGCAGGAGTCCCTGGCGACC
ATCGTCCAGGCGGTCAAGAGCGCCCTCGAGCAATCGCCGCCGGAGCTGGCCTCGGATATCGCCGAGCGCGGC
CTGGTGCTGACTGGCGGTGGCGCGCTGCTGCGCGACCTGGACAAGCTGCTGGCACAGGAAACCGGCCTGCCG
GTGATCGTCGCCGAGGAGCCGCTGACCTGCGTGGCTCGCGGCGGTGGCCGTGCGCTGGAGATGATGGACCGC
CACTCCATGGACCTGCTCTCCACCGAGTAA
Translation:
MFKKLRGMFSSDLSIDLGTANTLIYVRERGIVLNEPSVVAIRSHGSQKSVVAVGTEAKRMLGRTPGNIAAIR
PMKDGVIADFSVCEKMLQYFINKVHENSFLQPSPRVLICVPCKSTQVERRAIRESALGAGAREVFLIEEPMA
AAIGAGLPVEEARGSMVVDIGGGTTEIALISLNGVVYAESVRVGGDRFDEAIVTYVRRNYGSLIGESTAERI
KQEIGTAFPGGDVREVDVRGRNLAEGVPRSFTLNSNEVLEALQESLATIVQAVKSALEQSPPELASDIAERG
LVLTGGGALLRDLDKLLAQETGLPVIVAEEPLTCVARGGGRALEMMDRHSMDLLSTE*
AnnotationID:2088GeneID:2080
AnnotatorUID: diggins
Modification Date/Time: 2005-05-03 14:02:12
Gene Name:mreB
Confidence Code:2
GeneProduct:rod shape-determining protein MreB
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1077,pfam06723
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66558.1|AE016284_6 (AE016777) rod shape-determining protein MreB [Pseudomonas putida
           KT2440]
          Length = 372

 Score =  650 bits (1678), Expect = 0.0
 Identities = 336/345 (97%), Positives = 345/345 (100%)

>gb|AAO57921.1| (AE016872) rod shape-determining protein MreB [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 345

 Score =  650 bits (1677), Expect = 0.0
 Identities = 335/345 (97%), Positives = 345/345 (100%)

>gb|AAN82446.1|AE016767_206 (AE016767) Rod shape-determining protein mreB [Escherichia coli
           CFT073]
          Length = 372

 Score =  541 bits (1395), Expect = e-153
 Identities = 277/347 (79%), Positives = 310/347 (89%), Gaps = 2/347 (0%)







 
Structural Features:
COG1077, MreB, Actin-like ATPase involved in cell morphogenesis 
[Cell division and chromosome partitioning]
CD-Length = 342 residues, 100.0% aligned
Score =  486 bits (1252), Expect = 4e-138
Identities = 224/342 (65%), Positives = 276/342 (80%), Gaps = 2/342 (0%)

pfam06723, MreB_Mbl, MreB/Mbl protein.
CD-Length = 327 residues,  99.7% aligned
Score =  480 bits (1237), Expect = 2e-136
Identities = 214/329 (65%), Positives = 267/329 (81%), Gaps = 3/329 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4163




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21309




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31521




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2080_cdd.html


    		




 
URL:

















    ReferenceID:32876




  
Author/Investigator(s):
        Martin PR, Watson AA, McCaul TF, Mattick JS.





  
Title:
        Characterization of a five-gene cluster required for the biogenesis of type 4 fimbriae in Pseudomonas aeruginosa.




  
PubMed:
        7565110


MedLine:
        




    
Source:
Mol Microbiol. 1995 May;16(3):497-508.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7565110

















    ReferenceID:32877




  
Author/Investigator(s):
        Wachi M, Doi M, Ueda T, Ueki M, Tsuritani K, Nagai K, Matsuhashi M.





  
Title:
        Sequence of the downstream flanking region of the shape-determining genes mreBCD of Escherichia coli.




  
PubMed:
        1937035


MedLine:
        




    
Source:
Gene. 1991 Sep 30;106(1):135-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1937035

















    ReferenceID:32878




  
Author/Investigator(s):
        Wachi M, Matsuhashi M.





  
Title:
        Negative control of cell division by mreB, a gene that functions in determining the rod shape of Escherichia coli cells.




  
PubMed:
        2656641


MedLine:
        




    
Source:
J Bacteriol. 1989 Jun;171(6):3123-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2656641

















    ReferenceID:32879




  
Author/Investigator(s):
        Doi M, Wachi M, Ishino F, Tomioka S, Ito M, Sakagami Y, Suzuki A, Matsuhashi M.





  
Title:
        Determinations of the DNA sequence of the mreB gene and of the gene products of the mre region that function in formation of the rod shape of Escherichia coli cells.




  
PubMed:
        3049542


MedLine:
        




    
Source:
J Bacteriol. 1988 Oct;170(10):4619-24.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3049542

















    ReferenceID:32880




  
Author/Investigator(s):
        Kruse T, Moller-Jensen J, Lobner-Olesen A, Gerdes K.





  
Title:
        Dysfunctional MreB inhibits chromosome segregation in Escherichia coli.




  
PubMed:
        14517265


MedLine:
        




    
Source:
EMBO J. 2003 Oct 1;22(19):5283-92.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14517265

















    ReferenceID:32881




  
Author/Investigator(s):
        Lee JC, Cha JH, Zerbv DB, Stewart GC.





  
Title:
        Heterospecific expression of the Bacillus subtilis cell shape determination genes mreBCD in Escherichia coli.




  
PubMed:
        14506864


MedLine:
        




    
Source:
Curr Microbiol. 2003 Aug;47(2):146-52.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14506864









           
Homologs By Global Alignment 

           

       



     
Gene ID:2080
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3581
                           PA4481_tr
                           translation of PA4481
                           345
                           99.99
                           99.99
                           0
                           1707.0
     

     
     
109965
                           gb|AAG07869.1|AE004862_3
                           (AE004862) rod shape-determining protein MreB [Pseudomonas aeruginosa PAO1]
                           345
                           99.99
                           99.99
                           0
                           1707.0
     

     
     
109967
                           gb|AAO57921.1|
                           (AE016872) rod shape-determining protein MreB [Pseudomonas syringae pv. tomato str. DC3000]
                           345
                           97.10
                           99.99
                           0
                           1677.0
     

     
     
109968
                           gb|AAF22467.1|AF063934_1
                           (AF063934) rod shape determining protein MreB [Pseudomonas fluorescens]
                           346
                           95.95
                           99.13
                           1
                           1650.0
     

     
     
109966
                           gb|AAN66558.1|AE016284_6
                           (AE016777) rod shape-determining protein MreB [Pseudomonas putida KT2440]
                           372
                           90.32
                           92.74
                           27
                           1678.0
     

     






      

          
GC ORFID: 45082How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4481Glimmer Score: 

          
Start: 4440701Stop: 4439664

          
Length: 1038


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4481 translation of PA4481Homolog Bit Score 662.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5504 (ORF: BLASTX 650.0), t_PA4481 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTTCAAAAAATTGCGTGGCATGTTTTCCAGCGATCTGTCGATCGACCTGGGTACTGCCAATACCCTTATT 
TATGTGCGCGAGCGCGGCATCGTCCTGAACGAACCGTCCGTGGTCGCCATTCGCAGCCATGGCAGCCAGAAG 
AGCGTCGTCGCCGTGGGCACCGAGGCCAAGCGCATGCTCGGCCGTACCCCGGGCAACATCGCCGCCATCCGT 
CCGATGAAAGACGGCGTGATCGCGGATTTCAGCGTCTGCGAGAAGATGCTGCAGTACTTCATCAACAAGGTT 
CACGAGAACAGCTTCCTGCAGCCCAGCCCGCGCGTGCTGATCTGCGTGCCGTGCAAGTCCACCCAGGTTGAG 
CGCCGGGCCATCCGCGAATCCGCCCTGGGCGCCGGCGCCCGCGAGGTGTTCCTGATCGAAGAGCCGATGGCT 
GCTGCCATCGGCGCCGGCCTGCCGGTCGAGGAGGCCCGCGGCTCGATGGTCGTAGACATCGGCGGCGGCACT 
ACCGAGATCGCCCTGATTTCCCTCAACGGCGTGGTCTACGCCGAATCCGTCCGCGTCGGCGGCGACCGCTTC 
GACGAAGCCATCGTCACCTACGTGCGCCGCAACTACGGCAGCCTGATCGGCGAATCCACCGCCGAGCGCATC 
AAGCAGGAGATCGGCACCGCCTTCCCGGGCGGCGACGTTCGCGAAGTCGACGTCCGCGGCCGTAACCTGGCC 
GAAGGCGTACCGCGCAGCTTCACCCTGAACTCCAACGAGGTCCTCGAGGCCCTGCAGGAGTCCCTGGCGACC 
ATCGTCCAGGCGGTCAAGAGCGCCCTCGAGCAATCGCCGCCGGAGCTGGCCTCGGATATCGCCGAGCGCGGC 
CTGGTGCTGACTGGCGGTGGCGCGCTGCTGCGCGACCTGGACAAGCTGCTGGCACAGGAAACCGGCCTGCCG 
GTGATCGTCGCCGAGGAGCCGCTGACCTGCGTGGCTCGCGGCGGTGGCCGTGCGCTGGAGATGATGGACCGC 
CACTCCATGGACCTGCTCTCCACCGAGTAA