ViewGene
Locus Name:
GeneID:
Location:

GeneID:208
TrivialName: PA14_39590
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_39590
PAO1 Ortholog Locus:PA1927
Sequence Length:2301
Protein Length:766
Start: 3525752
Stop: 3523452
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05315.1|AE004619_2 (AE004619) 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 765/766 (99%), Positives = 765/766 (99%)
Sequence:
ATGGCCCTGGCCCACACTCTCGGCTTCCCACGCATCGGACGCGATCGCGAACTGAAAAAAGCCCAGGAAGCG
TTCTGGAAGGGCGAGCTGGACGAGGCCGGCCTGCGGGCCGTCGGCCGGCAGCTGCGCGCCGCCCACTGGCAG
GTACAGAAGGACGCCGGCATCCAGTTGCTGCCGGTCGGCGATTTCGCCTGGTATGACCAGGTGCTGACCCAT
TCGCTGACCTTCGGCGTGATCCCCGAGCGCTTCCGGGCGCACGGCGAGGCCGGGCCGACCCTGCATACCCTG
TTCGGCATGGCCCGTGGCGTCAGCGACGACAGTTGTTGCGGCGGTGCCCATGCCCAGGAAATGACCAAGTGG
TTCGACACCAACTATCACTACCTGGTCCCCGAGTTCAGCGCCGACCAGCAGTTCCAGCTGAGCTGGGAACAA
CTGTTCGAGGAGGTCGACGAGGCCAGGGCCCTCGGCCACCAGGTGAAGCCGGTGCTGATCGGTCCGCTGACC
TACCTGTGGCTGGGCAAGGCCAAGGGCGCCGAGTTCGACAGGCTCGACCTGCTGGACCGCCTGCTGCCGCTC
TACGGCCAGATCTTGCGTCGTCTCGCCGGCCAGGGCGTGGAGTGGGTGCAGATCGACGAGCCGATCCTGGTC
CTCGACCTGCCGCAGGCATGGAAGAACGCCTTCGAGCGCGCCTACAACCTGCTACAGAGCGAACCGCTGAAG
AAGCTGGTCGCCACCTACTTCGGCGGCCTGGAAGACAACCTGGGCCTGGCCGCCAACCTCCCGGTGGACGGC
CTGCACATCGACCTGGTGCGAGCGCCGGAACAGTATCCGAGCATCCTCGACCGCCTGCCGGCCTACAAGGTC
GTCTCGCTGGGCCTGGTCAACGGGCGCAACGTCTGGCGCTGCGACCTGGAGAAAGCCCTGGAGGTGGTGCGC
CATGCCCGGGAGCGCCTGGGCGAGCGGCTGTGGGTCGCGCCGTCGTGCTCGCTGCTGCACAGCCCGGTGGAC
CTGGAACGCGAAGACGGGCTGGACGCCGAGCTGAAGAGCTGGCTGGCCTTCGCCGTGCAGAAGTGCCGCGAA
GTCGCGGTGCTGGCCCGTGCCGCCACTGAGCCGGAAGCCGCCGAGGTGCTTGCCGCCCTGGAAGAGAGCCGT
GCGGTCCAGGCCAGCCGCGCTTCCTCGCCGCGAATCCACAAGCCGGCCGTGCAGGCGCGCCTGGCCGCGATC
AAGGCAAGCGACGCCCAGCGCCGCTCGCCGTTCGCCGAACGCATCGCCAGGCAGCGTGCCGGGCTCGACCTG
CCGGCGTTCCCGACCACCACCATCGGCTCCTTCCCGCAGACCTCGTCGATCCGCCTGGCCCGCCAGTCGTTC
AAGCAGGGCAAGCTGAGCGAGGCCGAGTACATCGAGGCGATGCACAGCGAGATCCGCCATGCGGTGCAGATC
CAGGAACAACTGGGGCTGGACGTGCTGGTACACGGCGAAGCCGAGCGCAACGACATGGTCGAGTACTTCGCC
GAGCAACTGGACGGCTACGTCTTCACCCGCTTCGGCTGGGTGCAGAGCTACGGCTCGCGCTGCGTGAAGCCG
GCGGTGATCTATGGCGACCTGAGCCGTCCGCGGGCCATGACCGTGGAGTGGATCAGGTACGCCCAGAGCCTT
ACCGACAAGGTGATGAAGGGCATGCTGACCGGCCCGGTGACCATGCTGATGTGGTCCTTCCCGCGCGAGGAC
GTGTCTCGCGAGGTGCAGGCGCGCCAGTTGGCCCTGGCGATCCGCGACGAAGTGGTCGACCTGGAGGCCGCC
GGCATCAGGATCGTGCAGATCGACGAGGCGGCTTTCCGCGAAGGCCTGCCGCTGCGCCGCAACGCCTGGCCG
CACTACCTGGAATGGGCCACCGAGGCCTTCCGCCTGTGCGCCTCCGGAGTCCGCGACGAAACCCAGATCCAT
ACGCACATGTGCTACAGCGAGTTCAACGATGTGATCGAGTCCATCGCCGCCATGGACGCCGACGTGATCACC
ATCGAGACCTCGCGTTCGGACATGGAGCTGCTGGAGGCCTTCGAGCAGTTCGACTACCCGAACGAGATCGGT
CCGGGCGTCTACGACATCCATTCGCCGCGCGTGCCGAGCCGGGAAGAGATCGTCGCGTTGCTGCGCAAGGCG
GCCCGGCGGATACCCGCCGAGCGGCTCTGGGTCAACCCGGACTGTGGCCTGAAGACCCGCGCCTGGCCGGAA
ACCGAGGCAGCGCTGGTGAACATGGTCGCCGCCGCCCGCGAACTGCGTGGCGACCTCGCCCGCGGCTGA
Translation:
MALAHTLGFPRIGRDRELKKAQEAFWKGELDEAGLRAVGRQLRAAHWQVQKDAGIQLLPVGDFAWYDQVLTH
SLTFGVIPERFRAHGEAGPTLHTLFGMARGVSDDSCCGGAHAQEMTKWFDTNYHYLVPEFSADQQFQLSWEQ
LFEEVDEARALGHQVKPVLIGPLTYLWLGKAKGAEFDRLDLLDRLLPLYGQILRRLAGQGVEWVQIDEPILV
LDLPQAWKNAFERAYNLLQSEPLKKLVATYFGGLEDNLGLAANLPVDGLHIDLVRAPEQYPSILDRLPAYKV
VSLGLVNGRNVWRCDLEKALEVVRHARERLGERLWVAPSCSLLHSPVDLEREDGLDAELKSWLAFAVQKCRE
VAVLARAATEPEAAEVLAALEESRAVQASRASSPRIHKPAVQARLAAIKASDAQRRSPFAERIARQRAGLDL
PAFPTTTIGSFPQTSSIRLARQSFKQGKLSEAEYIEAMHSEIRHAVQIQEQLGLDVLVHGEAERNDMVEYFA
EQLDGYVFTRFGWVQSYGSRCVKPAVIYGDLSRPRAMTVEWIRYAQSLTDKVMKGMLTGPVTMLMWSFPRED
VSREVQARQLALAIRDEVVDLEAAGIRIVQIDEAAFREGLPLRRNAWPHYLEWATEAFRLCASGVRDETQIH
THMCYSEFNDVIESIAAMDADVITIETSRSDMELLEAFEQFDYPNEIGPGVYDIHSPRVPSREEIVALLRKA
ARRIPAERLWVNPDCGLKTRAWPETEAALVNMVAAARELRGDLARG*
AnnotationID:218GeneID:208
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 14:02:37
Gene Name:metE
Confidence Code:2
GeneProduct:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:cobalamin-independent methionine synthase
Functional Category Confidence Code:5
COGs:pfam01717,COG0620
Secondary Functional Category(ies):
EC Number:2.1.1.14
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57635.1| (AE016871) 5-methyltetrahydropteroyltriglutamate--homocysteine
           S-methyltransferase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 766

 Score = 1236 bits (3198), Expect = 0.0
 Identities = 610/767 (79%), Positives = 672/767 (87%), Gaps = 9/767 (1%)

>gb|AAN83184.1|AE016769_299 (AE016769) 5-methyltetrahydropteroyltriglutamate--homocysteine
           methyltransferase [Escherichia coli CFT073]
          Length = 753

 Score =  935 bits (2416), Expect = 0.0
 Identities = 477/759 (62%), Positives = 570/759 (75%), Gaps = 12/759 (1%)

>gb|AAA23544.1| (M87625) cobalamin-independent methionine synthase [Escherichia
           coli]
          Length = 753

 Score =  932 bits (2409), Expect = 0.0
 Identities = 475/759 (62%), Positives = 570/759 (75%), Gaps = 12/759 (1%)








 
Structural Features:
pfam01717, Methionine_synt, Methionine synthase, vitamin-B12 independent. 
D-Length = 324 residues, 100.0% aligned
Score =  474 bits (1222), Expect = 1e-134
Identities = 201/324 (62%), Positives = 245/324 (75%)

COG0620, MetE, Methionine synthase II (cobalamin-independent) 
[Amino acid transport and metabolism]
CD-Length = 330 residues,  99.1% aligned
Score =  453 bits (1167), Expect = 3e-128
Identities = 207/329 (62%), Positives = 248/329 (75%), Gaps = 2/329 (0%)








   
Genomic Context:






   
Comment:


















    ReferenceID:419




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17561




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32784




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:208_cdd.html


    		




 
URL:

















    ReferenceID:32926




  
Author/Investigator(s):
        Fritsch PS, Urbanowski ML, Stauffer GV.





  
Title:
        Role of the RNA polymerase alpha subunits in MetR-dependent activation of metE and metH: important residues in the C-terminal domain and orientation requirements within RNA polymerase.




  
PubMed:
        10986259


MedLine:
        




    
Source:
J Bacteriol. 2000 Oct;182(19):5539-50.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10986259

















    ReferenceID:32927




  
Author/Investigator(s):
        Yocum RR, Perkins JB, Howitt CL, Pero J.





  
Title:
        Cloning and characterization of the metE gene encoding S-adenosylmethionine synthetase from Bacillus subtilis.




  
PubMed:
        8755891


MedLine:
        




    
Source:
J Bacteriol. 1996 Aug;178(15):4604-10.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8755891

















    ReferenceID:32928




  
Author/Investigator(s):
        Jafri S, Urbanowski ML, Stauffer GV.





  
Title:
        A mutation in the rpoA gene encoding the alpha subunit of RNA polymerase that affects metE-metR transcription in Escherichia coli.




  
PubMed:
        7836282


MedLine:
        




    
Source:
J Bacteriol. 1995 Feb;177(3):524-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7836282

















    ReferenceID:32929




  
Author/Investigator(s):
        Fonstein M, Nikolskaya T, Zaporojets D, Nikolsky Y, Kulakauskas S, Mironov A.





  
Title:
        Tn10-mediated inversions fuse uridine phosphorylase (udp) and rRNA genes of Escherichia coli.




  
PubMed:
        7512551


MedLine:
        




    
Source:
J Bacteriol. 1994 Apr;176(8):2265-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7512551

















    ReferenceID:32930




  
Author/Investigator(s):
        Gonzalez JC, Banerjee RV, Huang S, Sumner JS, Matthews RG.





  
Title:
        Comparison of cobalamin-independent and cobalamin-dependent methionine synthases from Escherichia coli: two solutions to the same chemical problem.




  
PubMed:
        1339288


MedLine:
        




    
Source:
Biochemistry. 1992 Jul 7;31(26):6045-56.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1339288

















    ReferenceID:32931




  
Author/Investigator(s):
        Weissbach H, Brot N.





  
Title:
        Regulation of methionine synthesis in Escherichia coli.




  
PubMed:
        1943695


MedLine:
        




    
Source:
Mol Microbiol. 1991 Jul;5(7):1593-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1943695

















    ReferenceID:32932




  
Author/Investigator(s):
        Cai XY, Redfield B, Maxon M, Weissbach H, Brot N.





  
Title:
        The effect of homocysteine on MetR regulation of metE, metR and metH expression in vitro.




  
PubMed:
        2673243


MedLine:
        




    
Source:
Biochem Biophys Res Commun. 1989 Aug 30;163(1):79-83.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2673243

















    ReferenceID:32933




  
Author/Investigator(s):
        Cai XY, Maxon ME, Redfield B, Glass R, Brot N, Weissbach H.





  
Title:
        Methionine synthesis in Escherichia coli: effect of the MetR protein on metE and metH expression.




  
PubMed:
        2543976


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1989 Jun;86(12):4407-11.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2543976

















    ReferenceID:32934




  
Author/Investigator(s):
        Lundrigan MD, De Veaux LC, Mann BJ, Kadner RJ.





  
Title:
        Separate regulatory systems for the repression of metE and btuB by vitamin B12 in Escherichia coli.




  
PubMed:
        3108627


MedLine:
        




    
Source:
Mol Gen Genet. 1987 Mar;206(3):401-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3108627

















    ReferenceID:32935




  
Author/Investigator(s):
        Chu J, Shoeman R, Hart J, Coleman T, Mazaitis A, Kelker N, Brot N, Weissbach H.





  
Title:
        Cloning and expression of the metE gene in Escherichia coli.




  
PubMed:
        2988449


MedLine:
        




    
Source:
Arch Biochem Biophys. 1985 Jun;239(2):467-74.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2988449









           
Homologs By Global Alignment 

           

       



     
Gene ID:208
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
358
                           PA1927_tr
                           translation of PA1927
                           766
                           99.86
                           99.86
                           0
                           3968.0
     

     
     
21879
                           gb|AAG05315.1|AE004619_2
                           (AE004619) 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase [Pseudomonas aeruginosa PAO1]
                           766
                           99.86
                           99.86
                           0
                           3968.0
     

     
     
21880
                           gb|AAO57635.1|
                           (AE016871) 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           773
                           79.17
                           87.06
                           14
                           3204.0
     

     
     
21881
                           gb|AAN83184.1|AE016769_299
                           (AE016769) 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [Escherichia coli CFT073]
                           769
                           62.41
                           74.77
                           19
                           2432.0
     

     
     
21894
                           gb|AAO70860.1|
                           (AE016845) 5-methyltetrahydropteroyltriglutamate- homocysteine methyltransferase [Salmonella enterica subsp. enterica serovar Typhi Ty2]
                           770
                           62.33
                           73.50
                           20
                           2409.0
     

     






      

          
GC ORFID: 43210How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1927Glimmer Score: 

          
Start: 1428159Stop: 1430459

          
Length: 2301


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1927 translation of PA1927Homolog Bit Score 1533.0

          
Other Homologs:  t_PA1927 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCCCTGGCCCACACTCTCGGCTTCCCACGCATCGGACGCGATCGCGAACTGAAAAAAGCCCAGGAAGCG 
TTCTGGAAGGGCGAGCTGGACGAGGCCGGCCTGCGGGCCGTCGGCCGGCAGCTGCGCGCCGCCCACTGGCAG 
GTACAGAAGGACGCCGGCATCCAGTTGCTGCCGGTCGGCGATTTCGCCTGGTATGACCAGGTGCTGACCCAT 
TCGCTGACCTTCGGCGTGATCCCCGAGCGCTTCCGGGCGCACGGCGAGGCCGGGCCGACCCTGCATACCCTG 
TTCGGCATGGCCCGTGGCGTCAGCGACGACAGTTGTTGCGGCGGTGCCCATGCCCAGGAAATGACCAAGTGG 
TTCGACACCAACTATCACTACCTGGTCCCCGAGTTCAGCGCCGACCAGCAGTTCCAGCTGAGCTGGGAACAA 
CTGTTCGAGGAGGTCGACGAGGCCAGGGCCCTCGGCCACCAGGTGAAGCCGGTGCTGATCGGTCCGCTGACC 
TACCTGTGGCTGGGCAAGGCCAAGGGCGCCGAGTTCGACAGGCTCGACCTGCTGGACCGCCTGCTGCCGCTC 
TACGGCCAGATCTTGCGTCGTCTCGCCGGCCAGGGCGTGGAGTGGGTGCAGATCGACGAGCCGATCCTGGTC 
CTCGACCTGCCGCAGGCATGGAAGAACGCCTTCGAGCGCGCCTACAACCTGCTACAGAGCGAACCGCTGAAG 
AAGCTGGTCGCCACCTACTTCGGCGGCCTGGAAGACAACCTGGGCCTGGCCGCCAACCTCCCGGTGGACGGC 
CTGCACATCGACCTGGTGCGAGCGCCGGAACAGTATCCGAGCATCCTCGACCGCCTGCCGGCCTACAAGGTC 
GTCTCGCTGGGCCTGGTCAACGGGCGCAACGTCTGGCGCTGCGACCTGGAGAAAGCCCTGGAGGTGGTGCGC 
CATGCCCGGGAGCGCCTGGGCGAGCGGCTGTGGGTCGCGCCGTCGTGCTCGCTGCTGCACAGCCCGGTGGAC 
CTGGAACGCGAAGACGGGCTGGACGCCGAGCTGAAGAGCTGGCTGGCCTTCGCCGTGCAGAAGTGCCGCGAA 
GTCGCGGTGCTGGCCCGTGCCGCCACTGAGCCGGAAGCCGCCGAGGTGCTTGCCGCCCTGGAAGAGAGCCGT 
GCGGTCCAGGCCAGCCGCGCTTCCTCGCCGCGAATCCACAAGCCGGCCGTGCAGGCGCGCCTGGCCGCGATC 
AAGGCAAGCGACGCCCAGCGCCGCTCGCCGTTCGCCGAACGCATCGCCAGGCAGCGTGCCGGGCTCGACCTG 
CCGGCGTTCCCGACCACCACCATCGGCTCCTTCCCGCAGACCTCGTCGATCCGCCTGGCCCGCCAGTCGTTC 
AAGCAGGGCAAGCTGAGCGAGGCCGAGTACATCGAGGCGATGCACAGCGAGATCCGCCATGCGGTGCAGATC 
CAGGAACAACTGGGGCTGGACGTGCTGGTACACGGCGAAGCCGAGCGCAACGACATGGTCGAGTACTTCGCC 
GAGCAACTGGACGGCTACGTCTTCACCCGCTTCGGCTGGGTGCAGAGCTACGGCTCGCGCTGCGTGAAGCCG 
GCGGTGATCTATGGCGACCTGAGCCGTCCGCGGGCCATGACCGTGGAGTGGATCAGGTACGCCCAGAGCCTT 
ACCGACAAGGTGATGAAGGGCATGCTGACCGGCCCGGTGACCATGCTGATGTGGTCCTTCCCGCGCGAGGAC 
GTGTCTCGCGAGGTGCAGGCGCGCCAGTTGGCCCTGGCGATCCGCGACGAAGTGGTCGACCTGGAGGCCGCC 
GGCATCAGGATCGTGCAGATCGACGAGGCGGCTTTCCGCGAAGGCCTGCCGCTGCGCCGCAACGCCTGGCCG 
CACTACCTGGAATGGGCCACCGAGGCCTTCCGCCTGTGCGCCTCCGGAGTCCGCGACGAAACCCAGATCCAT 
ACGCACATGTGCTACAGCGAGTTCAACGATGTGATCGAGTCCATCGCCGCCATGGACGCCGACGTGATCACC 
ATCGAGACCTCGCGTTCGGACATGGAGCTGCTGGAGGCCTTCGAGCAGTTCGACTACCCGAACGAGATCGGT 
CCGGGCGTCTACGACATCCATTCGCCGCGCGTGCCGAGCCGGGAAGAGATCGTCGCGTTGCTGCGCAAGGCG 
GCCCGGCGGATACCCGCCGAGCGGCTCTGGGTCAACCCGGACTGTGGCCTGAAGACCCGCGCCTGGCCGGAA 
ACCGAGGCAGCGCTGGTGAACATGGTCGCCGCCGCCCGCGAACTGCGTGGCGACCTCGCCCGCGGCTGA