ViewGene
Locus Name:
GeneID:
Location:

GeneID:2074
TrivialName: PA14_27420
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_27420
PAO1 Ortholog Locus:PA2836
Sequence Length:1065
Protein Length:354
Start: 2380234
Stop: 2379170
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06224.1|AE004710_6 (AE004710) probable secretion protein [Pseudomonas aeruginosa PAO1]
Identities = 351/354 (99%), Positives = 351/354 (99%)
Sequence:
ATGTCGTTCATCACTTCGCGCAAGGGCAGCCTGCTGCTCGCCGCCCTGCTGGTCCTGATCCTACTGGTCTAC
CTGCTGTTCCACCTGCTCGCCCCACGGGTCGTGCAGAGCACCGACGACGCCTATGTGCACGCCGATTTCACC
CTGGTCGCGCCGAAAGTCGCCGGCTTCGTCCAGGACGTCCTGGTCGAGGACAACCAGCCGGTCAAGGCCGGC
CAGTTGCTCGCCCGCCTCGACGACCGCGACTTCCGCACCGCCCTGGCCGCCGCCGAAGCCGACGTGCTCGGT
GCCGAGGCCAACCTGGCCAATGCCGAGGCCAACCTGCAACGCCAGCAGGCGCTGATCGAACAGGCGCGGGCC
AAGGTCCGCGCCGAGCAGGCCGAGCTGGTCTTCGCCCGTCACGAGCAAAGCCGCTACCAGACCCTCGCCAGC
AAGGGCGCCGGCAGCCTGCAGAACGCCCAGCAGGCGCAATCGCGGATCGATACCGCCAGCGCCCGGCTGGCC
GAGGGCCAGGCCGCGGTGGATGCCACGCGCAAGCAGGTGAGCGTCCTCGAAGCCCGCGTCGGCCAGGCCCGT
GGCGACCTGCAACGCATGCAGGCGCGACGCGACCAGGCCCGCCTGGACCTCTCCTACTGTGAACTCCGCGCG
CCCTTCGACGGCCTGGTCGGCCGCCGCCAACTGCGCGTCGGCGCCTATGTCACGCCGGGCAGCGCGCTGCTG
GCGGTGGTGCCCCTGCAGCAGGCCTACGTGGTCGGCAATTTCCAGGAAACCCAACTGACCCACGTCTCTCCC
GGACAGGCCGTGGAGATCCGCGTCGATACCTTCCCCGGCGAGGTTCTGCGCGGCCATGTCGACAGCATCGCA
CCGGCCACCGGCCTGAGCTTCGCGCCGATCGCCCCGGACAACGCCACCGGCAACTTCACCAAGGTGGTCCAG
CGGATCCCGGTGAAGATCGTTCTCGAGCCGCGCCAGGCCATGGCGGGCAAGTTGCGTGTGGGCATGTCGGTG
GAAGCACGGATCGACACCGCGACCGGCACCCACTCCGCCGAGGTCGCCCAGCAATGA
Translation:
MSFITSRKGSLLLAALLVLILLVYLLFHLLAPRVVQSTDDAYVHADFTLVAPKVAGFVQDVLVEDNQPVKAG
QLLARLDDRDFRTALAAAEADVLGAEANLANAEANLQRQQALIEQARAKVRAEQAELVFARHEQSRYQTLAS
KGAGSLQNAQQAQSRIDTASARLAEGQAAVDATRKQVSVLEARVGQARGDLQRMQARRDQARLDLSYCELRA
PFDGLVGRRQLRVGAYVTPGSALLAVVPLQQAYVVGNFQETQLTHVSPGQAVEIRVDTFPGEVLRGHVDSIA
PATGLSFAPIAPDNATGNFTKVVQRIPVKIVLEPRQAMAGKLRVGMSVEARIDTATGTHSAEVAQQ*
AnnotationID:2082GeneID:2074
AnnotatorUID: diggins
Modification Date/Time: 2005-06-11 08:08:44
Gene Name:
Confidence Code:3
GeneProduct:putative secretion protein
Cell Localization:()
Synonyms:trpB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:putative multidrug efflux pump
Functional Category Confidence Code:5
COGs:COG1566
Secondary Functional Category(ies):secreted factors (toxins, enzymes, alginate)
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAL95701.1|AF360362_3 (AF360362) TrpB [Pseudomonas putida]
          Length = 353

 Score =  388 bits (997), Expect = e-107
 Identities = 207/340 (60%), Positives = 259/340 (76%), Gaps = 1/340 (0%)

>gb|AAN66896.1|AE016318_3 (AE016778) multidrug efflux MFS membrane fusion protein, putative
           [Pseudomonas putida KT2440]
          Length = 367

 Score =  387 bits (994), Expect = e-107
 Identities = 207/340 (60%), Positives = 258/340 (75%), Gaps = 1/340 (0%)

>dbj|BAC52858.1| (AP005962) multidrug resistance efflux pump [Bradyrhizobium
           japonicum USDA 110]
          Length = 393

 Score =  325 bits (832), Expect = 3e-88
 Identities = 173/337 (51%), Positives = 234/337 (69%), Gaps = 2/337 (0%)









 
Structural Features:
COG1566, EmrA, Multidrug resistance efflux pump [Defense mechanisms]
CD-Length = 352 residues,  96.6% aligned
Score =  289 bits (742), Expect = 3e-79
Identities = 153/349 (43%), Positives = 209/349 (59%), Gaps = 10/349 (2%)








   
Genomic Context:






   
Comment:


















    ReferenceID:4151




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21297




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36219




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2074_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2074
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3574
                           PA2836_tr
                           translation of PA2836
                           354
                           99.15
                           99.15
                           0
                           1710.0
     

     
     
109869
                           gb|AAG06224.1|AE004710_6
                           (AE004710) probable secretion protein [Pseudomonas aeruginosa PAO1]
                           354
                           99.15
                           99.15
                           0
                           1710.0
     

     
     
109870
                           gb|AAL95701.1|AF360362_3
                           (AF360362) TrpB [Pseudomonas putida]
                           360
                           58.61
                           73.33
                           13
                           997.0
     

     
     
109871
                           gb|AAN66896.1|AE016318_3
                           (AE016778) multidrug efflux MFS membrane fusion protein, putative [Pseudomonas putida KT2440]
                           371
                           56.87
                           70.88
                           21
                           988.0
     

     
     
109875
                           emb|CAE92887.1|
                           (AJ617740) putative multidrug efflux pump [Pseudomonas putida]
                           357
                           46.21
                           65.26
                           16
                           797.0
     

     






      

          
GC ORFID: 45076How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2836Glimmer Score: 

          
Start: 2573693Stop: 2574757

          
Length: 1065


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2836 translation of PA2836Homolog Bit Score 663.0

          
Other Homologs:  t_PA1237 (ORF: BLASTX 257.0), t_PA2836 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCGTTCATCACTTCGCGCAAGGGCAGCCTGCTGCTCGCCGCCCTGCTGGTCCTGATCCTACTGGTCTAC 
CTGCTGTTCCACCTGCTCGCCCCACGGGTCGTGCAGAGCACCGACGACGCCTATGTGCACGCCGATTTCACC 
CTGGTCGCGCCGAAAGTCGCCGGCTTCGTCCAGGACGTCCTGGTCGAGGACAACCAGCCGGTCAAGGCCGGC 
CAGTTGCTCGCCCGCCTCGACGACCGCGACTTCCGCACCGCCCTGGCCGCCGCCGAAGCCGACGTGCTCGGT 
GCCGAGGCCAACCTGGCCAATGCCGAGGCCAACCTGCAACGCCAGCAGGCGCTGATCGAACAGGCGCGGGCC 
AAGGTCCGCGCCGAGCAGGCCGAGCTGGTCTTCGCCCGTCACGAGCAAAGCCGCTACCAGACCCTCGCCAGC 
AAGGGCGCCGGCAGCCTGCAGAACGCCCAGCAGGCGCAATCGCGGATCGATACCGCCAGCGCCCGGCTGGCC 
GAGGGCCAGGCCGCGGTGGATGCCACGCGCAAGCAGGTGAGCGTCCTCGAAGCCCGCGTCGGCCAGGCCCGT 
GGCGACCTGCAACGCATGCAGGCGCGACGCGACCAGGCCCGCCTGGACCTCTCCTACTGTGAACTCCGCGCG 
CCCTTCGACGGCCTGGTCGGCCGCCGCCAACTGCGCGTCGGCGCCTATGTCACGCCGGGCAGCGCGCTGCTG 
GCGGTGGTGCCCCTGCAGCAGGCCTACGTGGTCGGCAATTTCCAGGAAACCCAACTGACCCACGTCTCTCCC 
GGACAGGCCGTGGAGATCCGCGTCGATACCTTCCCCGGCGAGGTTCTGCGCGGCCATGTCGACAGCATCGCA 
CCGGCCACCGGCCTGAGCTTCGCGCCGATCGCCCCGGACAACGCCACCGGCAACTTCACCAAGGTGGTCCAG 
CGGATCCCGGTGAAGATCGTTCTCGAGCCGCGCCAGGCCATGGCGGGCAAGTTGCGTGTGGGCATGTCGGTG 
GAAGCACGGATCGACACCGCGACCGGCACCCACTCCGCCGAGGTCGCCCAGCAATGA