ViewGene
Locus Name:
GeneID:
Location:

GeneID:2072
TrivialName: PA14_48020
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_48020
PAO1 Ortholog Locus:PA1252
Sequence Length:1005
Protein Length:334
Start: 4275005
Stop: 4274001
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04641.1|AE004555_1 (AE004555) probable L-malate dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 334/334 (100%), Positives = 334/334 (100%)
Sequence:
GTGATCCGAATGACGCTGGACGAGGTCCGCGAGCTGGCCGTGCGCATCCTGCGCCGGCACGCTTTCAGCGAA
GCCCATGTACAGGCGGTGGCCGATACCCTGGTGGCGGGGGAGCGCGACGAATGCGCCTCCCACGGTATCTGG
CGGTTGCTCGGCTGCATCGCCACCCTGAAGGCCGGCAAGGTATCCGCCGACGCCGAGCCGGAACTGCACGAC
ATCGCTCCCGGCCTGCTGCGGGTCGACGCCCATGGCGGGTTCTCCCAGTGCGCATTCCGGCTGGGGCTGCCG
CATCTGCTGGAGAAGGCCCGCAGCCAGGGTATCGCGGCGATGGCGGTGAACCGCTGTGTGCATTTCTCCGCG
CTATGGGTTGAGGTCGAGGCACTCACCGAGGCGGGCCTGGTGGCCCTGGCGACCACGCCGAGTCATGCCTGG
GTGGCGCCGGCGGGCGGACGCAAGCCGATCTTCGGCACCAACCCGATCGCCTTTGGCTGGCCGCGTCCGGAC
GGCCCGCCGTTCGTCTTCGACTTCGCCACCAGCGCCGTGGCGCGTGGCGAGATCCAGTTGCACGAACGCGCC
GGCAAGCCAATCCCGCTGGGCTGGGGGGTGGACGAGCAGGGCGAGCCGACCACCGATGCCAGCGCCGCGTTG
CGAGGCGCCATGCTTACTTTCGGCGGGCACAAGGGCTCGGCCCTGGCGGCGATGGTCGAACTGCTCGCCGGT
CCGCTGATCGGCGACCTGACCAGTGCCGAGTCGCTGGCCTACGACGAGGGCAGCCGTTCTTCTCCCTACGGT
GGCGAACTGCTGATCGCCATCGATCCGCGGCGCATGCTCGGCGCCTCGGCGGAGGAGCACCTGGCGCGCGCC
GAGACGCTGTTCGAAGGCATCGTCGAACAGGGCGCGCGCTTGCCCTCGCAGCGACGCTTCGAAGCGCGCGAA
CGCAGCGCCAGGGACGGCGTGACGATTCCCGAGGCGTTGCACCGGGAGCTGCTGGCGTTGCTGGAGTGA
Translation:
MIRMTLDEVRELAVRILRRHAFSEAHVQAVADTLVAGERDECASHGIWRLLGCIATLKAGKVSADAEPELHD
IAPGLLRVDAHGGFSQCAFRLGLPHLLEKARSQGIAAMAVNRCVHFSALWVEVEALTEAGLVALATTPSHAW
VAPAGGRKPIFGTNPIAFGWPRPDGPPFVFDFATSAVARGEIQLHERAGKPIPLGWGVDEQGEPTTDASAAL
RGAMLTFGGHKGSALAAMVELLAGPLIGDLTSAESLAYDEGSRSSPYGGELLIAIDPRRMLGASAEEHLARA
ETLFEGIVEQGARLPSQRRFEARERSARDGVTIPEALHRELLALLE*
AnnotationID:2080GeneID:2072
AnnotatorUID: diggins
Modification Date/Time: 2005-05-24 21:09:26
Gene Name:
Confidence Code:3
GeneProduct:putative L-malate dehydrogenase
Cell Localization:(1) Cytoplasmic
Synonyms:PA1252
Cell Localization Confidence Code:3
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:putative malate/L-lactate dehydrogenase
Functional Category Confidence Code:5
COGs:pfam02615
Secondary Functional Category(ies):energy metabolism
EC Number:1.1.1.37
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAC48651.1| (AL603642) putative malate dehydrogenase protein [Sinorhizobium
           meliloti]
          Length = 345

 Score =  356 bits (914), Expect = 9e-98
 Identities = 183/331 (55%), Positives = 224/331 (67%), Gaps = 1/331 (0%)

>gb|AAN33442.1|AE014525_5 (AE014525) malate/L-lactate dehydrogenase family protein [Brucella
           suis 1330]
          Length = 345

 Score =  353 bits (905), Expect = 1e-96
 Identities = 179/322 (55%), Positives = 219/322 (68%), Gaps = 1/322 (0%)

>gb|AAL45470.1| (AE009396) malate dehydrogenase [Agrobacterium tumefaciens str. C58
           (U. Washington)]
          Length = 345

 Score =  329 bits (844), Expect = 1e-89
 Identities = 171/332 (51%), Positives = 214/332 (64%), Gaps = 1/332 (0%)








 
Structural Features:
pfam02615, Ldh_2, Malate/L-lactate dehydrogenase.
CD-Length = 334 residues, 100.0% aligned
Score =  197 bits (501), Expect = 2e-51
Identities = 156/339 (46%), Positives = 194/339 (57%), Gaps = 5/339 (1%)







   
Genomic Context:






   
Comment:


















    ReferenceID:14497




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21293




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37537




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2072_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2072
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3572
                           PA1252_tr
                           translation of PA1252
                           334
                           99.99
                           99.99
                           0
                           1712.0
     

     
     
109854
                           gb|AAG04641.1|AE004555_1
                           (AE004555) probable L-malate dehydrogenase [Pseudomonas aeruginosa PAO1]
                           334
                           99.70
                           99.99
                           0
                           1709.0
     

     
     
109855
                           emb|CAC48651.1|
                           (AL603642) putative malate dehydrogenase protein [Sinorhizobium meliloti]
                           345
                           53.04
                           64.92
                           11
                           913.0
     

     
     
109856
                           gb|AAN33442.1|AE014525_5
                           (AE014525) malate/L-lactate dehydrogenase family protein [Brucella suis 1330]
                           345
                           52.75
                           64.34
                           11
                           900.0
     

     
     
109857
                           gb|AAL54247.1|
                           (AE009733) MALATE DEHYDROGENASE [Brucella melitensis 16M]
                           345
                           52.46
                           64.34
                           11
                           899.0
     

     






      

          
GC ORFID: 45074How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1252Glimmer Score: 

          
Start: 678904Stop: 679908

          
Length: 1005


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1252 translation of PA1252Homolog Bit Score 664.0

          
Other Homologs:  t_PA1252 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGATCCGAATGACGCTGGACGAGGTCCGCGAGCTGGCCGTGCGCATCCTGCGCCGGCACGCTTTCAGCGAA 
GCCCATGTACAGGCGGTGGCCGATACCCTGGTGGCGGGGGAGCGCGACGAATGCGCCTCCCACGGTATCTGG 
CGGTTGCTCGGCTGCATCGCCACCCTGAAGGCCGGCAAGGTATCCGCCGACGCCGAGCCGGAACTGCACGAC 
ATCGCTCCCGGCCTGCTGCGGGTCGACGCCCATGGCGGGTTCTCCCAGTGCGCATTCCGGCTGGGGCTGCCG 
CATCTGCTGGAGAAGGCCCGCAGCCAGGGTATCGCGGCGATGGCGGTGAACCGCTGTGTGCATTTCTCCGCG 
CTATGGGTTGAGGTCGAGGCACTCACCGAGGCGGGCCTGGTGGCCCTGGCGACCACGCCGAGTCATGCCTGG 
GTGGCGCCGGCGGGCGGACGCAAGCCGATCTTCGGCACCAACCCGATCGCCTTTGGCTGGCCGCGTCCGGAC 
GGCCCGCCGTTCGTCTTCGACTTCGCCACCAGCGCCGTGGCGCGTGGCGAGATCCAGTTGCACGAACGCGCC 
GGCAAGCCAATCCCGCTGGGCTGGGGGGTGGACGAGCAGGGCGAGCCGACCACCGATGCCAGCGCCGCGTTG 
CGAGGCGCCATGCTTACTTTCGGCGGGCACAAGGGCTCGGCCCTGGCGGCGATGGTCGAACTGCTCGCCGGT 
CCGCTGATCGGCGACCTGACCAGTGCCGAGTCGCTGGCCTACGACGAGGGCAGCCGTTCTTCTCCCTACGGT 
GGCGAACTGCTGATCGCCATCGATCCGCGGCGCATGCTCGGCGCCTCGGCGGAGGAGCACCTGGCGCGCGCC 
GAGACGCTGTTCGAAGGCATCGTCGAACAGGGCGCGCGCTTGCCCTCGCAGCGACGCTTCGAAGCGCGCGAA 
CGCAGCGCCAGGGACGGCGTGACGATTCCCGAGGCGTTGCACCGGGAGCTGCTGGCGTTGCTGGAGTGA