ViewGene
Locus Name:
GeneID:
Location:

GeneID:2068
TrivialName: PA14_56720
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_56720
PAO1 Ortholog Locus:PA4361
Sequence Length:990
Protein Length:329
Start: 5053653
Stop: 5052664
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07749.1|AE004852_2 (AE004852) probable oxidoreductase [Pseudomonas aeruginosa PAO1]
Identities = 328/329 (99%), Positives = 329/329 (100%)
Sequence:
ATGCGGATTCTGGTAACGGGGGCGACGGGCTTCATCGGCGGGCGTTTCGCTCGCTTCGCCCTGGAGCAGGGC
CTGTCGGTACGGGTCAGCGGTCGTCGTGCGGACGCGGTGGAGCATCTGGTGGCGCGGGGCGCCGAGTTCGTT
CCCGGCGACCTCGCCGACCCGGCGCTGGTCCTGCGCCTCTGCGAGGATGTCGAGGCGGTGGTGCATTGTGCC
GGCGCGGTCGGAGTCTGGGGGCCGCGCGAACGCTTCCTGGCGGCCAACGTCGGGCTTGCCGAGAGCGTCGTC
GAGGCCTGCATGCGGCAAAAGGTGCGGCGCCTGGTGCATCTGTCGTCGCCGTCGATCTATTTCGACGGGCGC
GACCACCTGGACCTGAACGAAGAGTACGTACCCCGGCGTTTTTCCGACCATTACGGGGCGACCAAGTACCAG
GCCGAGCAACTGGTGCTCAGCGCCCGCGACCTGGGCCTGGAAGTGTTGGCGCTGCGTCCGCGCTTCGTGGTC
GGTGCCGGCGACACCAGCATCTTCCCGCGAATGATCCAGGCCCACCGCAAGGGACGCCTGCGGATCCTCGGC
AACGGCCTGAACCGGGTCGACTTCACCAGCGTGCACAATCTCAACGACGCGCTGTTCAGTTGCCTGCTGGCC
GGCGAGCCGGCGCTGGGCAAGGTCTACAACATCAGCAACGGGCAGCCGGTACCGTTCTGGGATGCGGTCAAC
TACGTGATGCGCCAGCTCGAGCTGCCGCCGGTCGGCGGTCACCTGCCGTATGCCGTCGGCTATGGCCTGGCG
GCGCTCAACGAAGGCGTCTGCCGGATCCTGCCGGGGCGTCCGGAGCCGGTGCTGTTCCGCCTGGGCATGGCG
GTGATGGCGAAGAACTTCACCCTGGACATCAACCGCGCCCGCGAATACCTCGACTACGACCCGCGGGTCAGC
CTGTGGACCGCGCTCGACGAGTTCTGCGCCTGGTGGCGCGCCCAGGGACTCTGA
Translation:
MRILVTGATGFIGGRFARFALEQGLSVRVSGRRADAVEHLVARGAEFVPGDLADPALVLRLCEDVEAVVHCA
GAVGVWGPRERFLAANVGLAESVVEACMRQKVRRLVHLSSPSIYFDGRDHLDLNEEYVPRRFSDHYGATKYQ
AEQLVLSARDLGLEVLALRPRFVVGAGDTSIFPRMIQAHRKGRLRILGNGLNRVDFTSVHNLNDALFSCLLA
GEPALGKVYNISNGQPVPFWDAVNYVMRQLELPPVGGHLPYAVGYGLAALNEGVCRILPGRPEPVLFRLGMA
VMAKNFTLDINRAREYLDYDPRVSLWTALDEFCAWWRAQGL*
AnnotationID:2076GeneID:2068
AnnotatorUID: diggins
Modification Date/Time: 2005-05-20 12:12:56
Gene Name:
Confidence Code:3
GeneProduct:probable dehydrogenase
Cell Localization:()
Synonyms:PA4361
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:KOG1430,COG0451
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66543.1|AE016283_2 (AE016777) 3-beta hydroxysteroid dehydrogenase/isomerase family
           protein, putative [Pseudomonas putida KT2440]
          Length = 330

 Score =  470 bits (1209), Expect = e-132
 Identities = 224/325 (68%), Positives = 262/325 (80%)

>gb|AAO57810.1| (AE016871) dehydrogenase, putative [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 306

 Score =  431 bits (1107), Expect = e-120
 Identities = 206/303 (67%), Positives = 240/303 (79%)







 
Structural Features:
KOG1430, C-3 sterol dehydrogenase/3-beta-hydroxysteroid dehydrogenase 
and related dehydrogenases 
[Lipid transport and metabolism, Amino acid transport and metabolism]
CD-Length = 361 residues,  93.9% aligned
Score =  162 bits (412), Expect = 9e-41
Identities = 107/344 (31%), Positives = 162/344 (47%), Gaps = 27/344 (7%)

COG0451, WcaG, Nucleoside-diphosphate-sugar epimerases 
[Cell envelope biogenesis, outer membrane / 
Carbohydrate transport and metabolism]
CD-Length = 314 residues,  99.4% aligned
Score =  158 bits (401), Expect = 1e-39
Identities = 103/342 (30%), Positives = 145/342 (42%), Gaps = 44/342 (12%)







   
Genomic Context:






   
Comment:


















    ReferenceID:4139




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17112




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2068_cdd.html


    		




 
URL:

















    ReferenceID:21285




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2068
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3568
                           PA4361_tr
                           translation of PA4361
                           329
                           99.69
                           99.99
                           0
                           1717.0
     

     
     
109817
                           gb|AAG07749.1|AE004852_2
                           (AE004852) probable oxidoreductase [Pseudomonas aeruginosa PAO1]
                           329
                           99.69
                           99.99
                           0
                           1717.0
     

     
     
109818
                           gb|AAN66543.1|AE016283_2
                           (AE016777) 3-beta hydroxysteroid dehydrogenase/isomerase family protein, putative [Pseudomonas putida KT2440]
                           331
                           67.97
                           79.45
                           3
                           1202.0
     

     
     
109819
                           gb|AAO57810.1|
                           (AE016871) dehydrogenase, putative [Pseudomonas syringae pv. tomato str. DC3000]
                           330
                           62.42
                           72.72
                           25
                           1104.0
     

     






      

          
GC ORFID: 45070How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4361Glimmer Score: 

          
Start: 4317329Stop: 4316340

          
Length: 990


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4361 translation of PA4361Homolog Bit Score 665.0

          
Other Homologs:  psyr_15may02_Scaffold12_revised_gene2067 (ORF: BLASTX 471.0), t_PA4361 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGGATTCTGGTAACGGGGGCGACGGGCTTCATCGGCGGGCGTTTCGCTCGCTTCGCCCTGGAGCAGGGC 
CTGTCGGTACGGGTCAGCGGTCGTCGTGCGGACGCGGTGGAGCATCTGGTGGCGCGGGGCGCCGAGTTCGTT 
CCCGGCGACCTCGCCGACCCGGCGCTGGTCCTGCGCCTCTGCGAGGATGTCGAGGCGGTGGTGCATTGTGCC 
GGCGCGGTCGGAGTCTGGGGGCCGCGCGAACGCTTCCTGGCGGCCAACGTCGGGCTTGCCGAGAGCGTCGTC 
GAGGCCTGCATGCGGCAAAAGGTGCGGCGCCTGGTGCATCTGTCGTCGCCGTCGATCTATTTCGACGGGCGC 
GACCACCTGGACCTGAACGAAGAGTACGTACCCCGGCGTTTTTCCGACCATTACGGGGCGACCAAGTACCAG 
GCCGAGCAACTGGTGCTCAGCGCCCGCGACCTGGGCCTGGAAGTGTTGGCGCTGCGTCCGCGCTTCGTGGTC 
GGTGCCGGCGACACCAGCATCTTCCCGCGAATGATCCAGGCCCACCGCAAGGGACGCCTGCGGATCCTCGGC 
AACGGCCTGAACCGGGTCGACTTCACCAGCGTGCACAATCTCAACGACGCGCTGTTCAGTTGCCTGCTGGCC 
GGCGAGCCGGCGCTGGGCAAGGTCTACAACATCAGCAACGGGCAGCCGGTACCGTTCTGGGATGCGGTCAAC 
TACGTGATGCGCCAGCTCGAGCTGCCGCCGGTCGGCGGTCACCTGCCGTATGCCGTCGGCTATGGCCTGGCG 
GCGCTCAACGAAGGCGTCTGCCGGATCCTGCCGGGGCGTCCGGAGCCGGTGCTGTTCCGCCTGGGCATGGCG 
GTGATGGCGAAGAACTTCACCCTGGACATCAACCGCGCCCGCGAATACCTCGACTACGACCCGCGGGTCAGC 
CTGTGGACCGCGCTCGACGAGTTCTGCGCCTGGTGGCGCGCCCAGGGACTCTGA