ViewGene
Locus Name:
GeneID:
Location:

GeneID:205
TrivialName: PA14_62200
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62200
PAO1 Ortholog Locus:PA4700
Sequence Length:2325
Protein Length:774
Start: 5552224
Stop: 5549900
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08086.1|AE004884_4 (AE004884) penicillin-binding protein 1B [Pseudomonas aeruginosa PAO1]
Identities = 772/774 (99%), Positives = 772/774 (99%)
Sequence:
ATGACGCGTCCCCGATCCCCCCGTTCCCGTAACAGCAAAGCACGCCCGGCACCCGGCCTGAACAAATGGCTC
GGCTGGGCCCTCAAGCTCGGCCTCGTCGGCCTGGTGCTCCTCGCCGGCTTCGCCATCTATCTCGATGCCGTG
GTGCAGGAGAAATTCTCCGGCCGGCGCTGGACCATCCCGGCGAAGGTCTATGCCCGCCCGCTGGAGCTGTTC
AACGGCCTGAAGCTGAGCAGGGAAGACTTCCTGCGCGAACTCGACGCCCTCGGCTACCGTCGCGAGCCCAGC
GTGAGCGGCCCTGGAACCGTGTCGGTGGCGGCCAGCGCGGTCGAACTCAATACCCGTGGCTTCCAGTTCTAC
GAAGGCGCGGAACCGGCCCAGCGCGTGCGCGTGCGCTTCAACGGCAACTACGTCAGCGGGCTGAGCCAGGCC
AACGGCAAGGAACTGGCGGTGGCGCGCCTTGAGCCGCTGCTGATCGGCGGCTTGTATCCGGCGCACCATGAG
GATCGCATCCTGGTCAAGCTCGACCAGGTGCCGACCTACCTGATCGACACCCTGGTCGCGGTCGAGGATCGC
GACTTCTGGAACCACCATGGCGTGTCGCTGAAGTCCGTGGCCCGGGCGGTGTGGGTCAACACCACGGCCGGC
CAGCTGCGCCAGGGCGGCAGCACCCTGACCCAGCAGTTGGTGAAGAACTTCTTCCTGAGCAACGAACGCAGC
CTGAGCCGCAAGATCAACGAAGCGATGATGGCGGTGCTGCTGGAGCTGCACTACGACAAGCGCGACATCCTC
GAGTCCTATCTCAACGAGGTGTTCCTCGGCCAGGATGGGCAACGAGCGATCCATGGCTTCGGCCTGGCCAGC
CAGTATTTCTTCAGCCAGCCGCTGGCCGAGCTCAAGCTCGACCAGGTGGCGCTGCTGGTCGGCATGGTCAAG
GGACCGTCCTACTTCAACCCGCGGCGTTACCCGGATCGTGCGCTGGCGCGGCGCAACCTGGTGCTCGATGTG
CTCGCCGAGCAGGGCGTGGCGACCCAGCAGGAAGTCGACGCCGCCAAGCAGCGCCCGCTCGGCGTGACCCGG
CAGGGCAGCATGGCCGACAGCTCCTATCCGGCCTTCCTCGACCTGGTCAAGCGCCAGTTGCGCCAGGACTAC
CGCGACGAGGACCTGACCGAGGAAGGGCTGCGCATCTTCACCAGCTTCGATCCGATCCTGCAGGAGAAGGCC
GAGACCTCCGTCAACGAAACGCTCAAGCGCCTCAGTGGGCGCAAGGGAGTCGACCAGGTCGAGGCGGCGATG
GTCGTGACCAATCCGGAAACCGGCGAGATCCAGGCCCTGATCGGCAGTCGCGACCCGCGCTTCGCCGGCTTC
AACCGGGCCCTGGACGCGGTGCGGCCGATCGGTTCGCTGATCAAGCCGGCGGTCTACCTGACCGCACTCGAG
CGGCCGAGCAAGTACACCCTGACCACCTGGGTGCAGGACGAACCCTTCGCGGTGAAAGGCCAGGACGGCCAG
GTCTGGCGACCGCAGAACTACGACCGGCGCTCGCATGGCACGATCTTCCTGTACCAGGGCCTGGCAAACTCC
TACAACCTGTCCACCGCCAAGCTCGGCCTCGACGTCGGTGTGCCCAACGTGCTGCAGACCGTCGCCCGGCTG
GGCATCAACCGTGACTGGCCAGCCTATCCGTCGATGCTGCTGGGCGCCGGTTCGCTCAGTCCGATGGAGGTC
GCCACCATGTACCAGACCATCGCCAGCGGCGGCTTCAACACGCCGTTGCGGGGGATCCGCAGCGTCCTCACT
GCCGACGGCCAGCCGCTCAAGCGCTATCCGTTCCAGGTCGAACAGCGCTTCGACTCGGGCGCCGTCTACCTG
GTGCAGAACGCCATGCAGCGGGTCATGCGCGAAGGCACCGGGCGGTCGGTGTACAGTCAATTGCCGTCGTCG
CTGACCCTCGCCGGCAAGACCGGCACCAGCAACGACTCGCGCGATAGCTGGTTCTCCGGTTTCGGCGGCGAC
CTCCAGGCTGTGGTCTGGCTCGGTCGCGACGACAACGGCAAGACGCCGCTTACCGGCGCCACCGGTGCCTTG
CAGGTGTGGGCCAGCTTCATGCGCAAGGCGCATCCGCAATCGCTGGAAATGCCGATGCCGGAGAACGTGGTG
ATGGCCTGGGTCGACGCGCAGACCGGGCAGGGCTCGGCGGCGGATTGCCCGAATGCCGTACAGATGCCCTAT
ATTCGTGGCAGCGAGCCCGCCCAGGGCCCAGGCTGCGGGAGCCAGAACCCCGCCGGCGAGGTGATGGACTGG
GTACGAGGCTGGCTGAATTGA
Translation:
MTRPRSPRSRNSKARPAPGLNKWLGWALKLGLVGLVLLAGFAIYLDAVVQEKFSGRRWTIPAKVYARPLELF
NGLKLSREDFLRELDALGYRREPSVSGPGTVSVAASAVELNTRGFQFYEGAEPAQRVRVRFNGNYVSGLSQA
NGKELAVARLEPLLIGGLYPAHHEDRILVKLDQVPTYLIDTLVAVEDRDFWNHHGVSLKSVARAVWVNTTAG
QLRQGGSTLTQQLVKNFFLSNERSLSRKINEAMMAVLLELHYDKRDILESYLNEVFLGQDGQRAIHGFGLAS
QYFFSQPLAELKLDQVALLVGMVKGPSYFNPRRYPDRALARRNLVLDVLAEQGVATQQEVDAAKQRPLGVTR
QGSMADSSYPAFLDLVKRQLRQDYRDEDLTEEGLRIFTSFDPILQEKAETSVNETLKRLSGRKGVDQVEAAM
VVTNPETGEIQALIGSRDPRFAGFNRALDAVRPIGSLIKPAVYLTALERPSKYTLTTWVQDEPFAVKGQDGQ
VWRPQNYDRRSHGTIFLYQGLANSYNLSTAKLGLDVGVPNVLQTVARLGINRDWPAYPSMLLGAGSLSPMEV
ATMYQTIASGGFNTPLRGIRSVLTADGQPLKRYPFQVEQRFDSGAVYLVQNAMQRVMREGTGRSVYSQLPSS
LTLAGKTGTSNDSRDSWFSGFGGDLQAVVWLGRDDNGKTPLTGATGALQVWASFMRKAHPQSLEMPMPENVV
MAWVDAQTGQGSAADCPNAVQMPYIRGSEPAQGPGCGSQNPAGEVMDWVRGWLN*
AnnotationID:215GeneID:205
AnnotatorUID: diggins
Modification Date/Time: 2005-05-08 17:05:33
Gene Name:mrcB
Confidence Code:2
GeneProduct:penicillin-binding protein 1B
Cell Localization:()
Synonyms:ponB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0744,COG4953
Secondary Functional Category(ies):
EC Number:2.4.1.129
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70256.1|AE016665_9 (AE016791) penicillin-binding protein [Pseudomonas putida KT2440]
          Length = 773

 Score = 1249 bits (3232), Expect = 0.0
 Identities = 605/775 (78%), Positives = 693/775 (89%), Gaps = 3/775 (0%)

>gb|AAO54511.1| (AE016859) penicillin-binding protein [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 773

 Score = 1219 bits (3154), Expect = 0.0
 Identities = 592/775 (76%), Positives = 687/775 (88%), Gaps = 3/775 (0%)

>gb|AAN78677.1|AE016755_177 (AE016755) Penicillin-binding protein 1B [Escherichia coli CFT073]
          Length = 844

 Score =  567 bits (1461), Expect = e-160
 Identities = 309/726 (42%), Positives = 451/726 (62%), Gaps = 7/726 (0%)








 
Structural Features:
COG0744, MrcB, Membrane carboxypeptidase (penicillin-binding protein) 
[Cell envelope biogenesis, outer membrane]
CD-Length = 661 residues,  96.4% aligned
Score =  551 bits (1421), Expect = 1e-157
Identities = 249/662 (37%), Positives = 344/662 (51%), Gaps = 28/662 (4%)

COG4953, PbpC, Membrane carboxypeptidase/penicillin-binding protein PbpC 
[Cell envelope biogenesis, outer membrane]
CD-Length = 733 residues,  73.7% aligned
Score =  261 bits (669), Expect = 2e-70
Identities = 164/581 (28%), Positives = 247/581 (42%), Gaps = 50/581 (8%)






   
Genomic Context:






   
Comment:


















    ReferenceID:413




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17555




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37622




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:205_cdd.html


    		




 
URL:

















    ReferenceID:37749




  
Author/Investigator(s):
        Pla J, Rojo F, de Pedro MA, Ayala JA.





  
Title:
        Cloning and expression of the ponB gene, encoding penicillin-binding protein 1B of Escherichia coli, in heterologous systems.




  
PubMed:
        2198260


MedLine:
        




    
Source:
J Bacteriol. 1990 Aug;172(8):4448-55.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2198260

















    ReferenceID:37750




  
Author/Investigator(s):
        Broome-Smith JK, Edelman A, Yousif S, Spratt BG.





  
Title:
        The nucleotide sequences of the ponA and ponB genes encoding penicillin-binding protein 1A and 1B of Escherichia coli K12.




  
PubMed:
        3882429


MedLine:
        




    
Source:
Eur J Biochem. 1985 Mar 1;147(2):437-46.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3882429









           
Homologs By Global Alignment 

           

       



     
Gene ID:205
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
347
                           PA4700_tr
                           translation of PA4700
                           774
                           99.74
                           99.74
                           0
                           3987.0
     

     
     
21734
                           gb|AAG08086.1|AE004884_4
                           (AE004884) penicillin-binding protein 1B [Pseudomonas aeruginosa PAO1]
                           774
                           99.74
                           99.74
                           0
                           3987.0
     

     
     
21735
                           gb|AAD32237.1|AF147449_1
                           (AF147449) penicillin-binding protein 1B [Pseudomonas aeruginosa]
                           774
                           99.74
                           99.74
                           0
                           3987.0
     

     
     
21736
                           gb|AAN70256.1|AE016665_9
                           (AE016791) penicillin-binding protein [Pseudomonas putida KT2440]
                           775
                           78.06
                           89.41
                           3
                           3236.0
     

     
     
21737
                           gb|AAO54511.1|
                           (AE016859) penicillin-binding protein [Pseudomonas syringae pv. tomato str. DC3000]
                           775
                           76.38
                           88.64
                           3
                           3158.0
     

     






      

          
GC ORFID: 43207How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4700Glimmer Score: 

          
Start: 4815904Stop: 4813580

          
Length: 2325


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4700 translation of PA4700Homolog Bit Score 1540.0

          
Other Homologs:  psyr_15may02_Scaffold19_revised_gene2870 (ORF: BLASTX 1222.0), t_PA4700 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACGCGTCCCCGATCCCCCCGTTCCCGTAACAGCAAAGCACGCCCGGCACCCGGCCTGAACAAATGGCTC 
GGCTGGGCCCTCAAGCTCGGCCTCGTCGGCCTGGTGCTCCTCGCCGGCTTCGCCATCTATCTCGATGCCGTG 
GTGCAGGAGAAATTCTCCGGCCGGCGCTGGACCATCCCGGCGAAGGTCTATGCCCGCCCGCTGGAGCTGTTC 
AACGGCCTGAAGCTGAGCAGGGAAGACTTCCTGCGCGAACTCGACGCCCTCGGCTACCGTCGCGAGCCCAGC 
GTGAGCGGCCCTGGAACCGTGTCGGTGGCGGCCAGCGCGGTCGAACTCAATACCCGTGGCTTCCAGTTCTAC 
GAAGGCGCGGAACCGGCCCAGCGCGTGCGCGTGCGCTTCAACGGCAACTACGTCAGCGGGCTGAGCCAGGCC 
AACGGCAAGGAACTGGCGGTGGCGCGCCTTGAGCCGCTGCTGATCGGCGGCTTGTATCCGGCGCACCATGAG 
GATCGCATCCTGGTCAAGCTCGACCAGGTGCCGACCTACCTGATCGACACCCTGGTCGCGGTCGAGGATCGC 
GACTTCTGGAACCACCATGGCGTGTCGCTGAAGTCCGTGGCCCGGGCGGTGTGGGTCAACACCACGGCCGGC 
CAGCTGCGCCAGGGCGGCAGCACCCTGACCCAGCAGTTGGTGAAGAACTTCTTCCTGAGCAACGAACGCAGC 
CTGAGCCGCAAGATCAACGAAGCGATGATGGCGGTGCTGCTGGAGCTGCACTACGACAAGCGCGACATCCTC 
GAGTCCTATCTCAACGAGGTGTTCCTCGGCCAGGATGGGCAACGAGCGATCCATGGCTTCGGCCTGGCCAGC 
CAGTATTTCTTCAGCCAGCCGCTGGCCGAGCTCAAGCTCGACCAGGTGGCGCTGCTGGTCGGCATGGTCAAG 
GGACCGTCCTACTTCAACCCGCGGCGTTACCCGGATCGTGCGCTGGCGCGGCGCAACCTGGTGCTCGATGTG 
CTCGCCGAGCAGGGCGTGGCGACCCAGCAGGAAGTCGACGCCGCCAAGCAGCGCCCGCTCGGCGTGACCCGG 
CAGGGCAGCATGGCCGACAGCTCCTATCCGGCCTTCCTCGACCTGGTCAAGCGCCAGTTGCGCCAGGACTAC 
CGCGACGAGGACCTGACCGAGGAAGGGCTGCGCATCTTCACCAGCTTCGATCCGATCCTGCAGGAGAAGGCC 
GAGACCTCCGTCAACGAAACGCTCAAGCGCCTCAGTGGGCGCAAGGGAGTCGACCAGGTCGAGGCGGCGATG 
GTCGTGACCAATCCGGAAACCGGCGAGATCCAGGCCCTGATCGGCAGTCGCGACCCGCGCTTCGCCGGCTTC 
AACCGGGCCCTGGACGCGGTGCGGCCGATCGGTTCGCTGATCAAGCCGGCGGTCTACCTGACCGCACTCGAG 
CGGCCGAGCAAGTACACCCTGACCACCTGGGTGCAGGACGAACCCTTCGCGGTGAAAGGCCAGGACGGCCAG 
GTCTGGCGACCGCAGAACTACGACCGGCGCTCGCATGGCACGATCTTCCTGTACCAGGGCCTGGCAAACTCC 
TACAACCTGTCCACCGCCAAGCTCGGCCTCGACGTCGGTGTGCCCAACGTGCTGCAGACCGTCGCCCGGCTG 
GGCATCAACCGTGACTGGCCAGCCTATCCGTCGATGCTGCTGGGCGCCGGTTCGCTCAGTCCGATGGAGGTC 
GCCACCATGTACCAGACCATCGCCAGCGGCGGCTTCAACACGCCGTTGCGGGGGATCCGCAGCGTCCTCACT 
GCCGACGGCCAGCCGCTCAAGCGCTATCCGTTCCAGGTCGAACAGCGCTTCGACTCGGGCGCCGTCTACCTG 
GTGCAGAACGCCATGCAGCGGGTCATGCGCGAAGGCACCGGGCGGTCGGTGTACAGTCAATTGCCGTCGTCG 
CTGACCCTCGCCGGCAAGACCGGCACCAGCAACGACTCGCGCGATAGCTGGTTCTCCGGTTTCGGCGGCGAC 
CTCCAGGCTGTGGTCTGGCTCGGTCGCGACGACAACGGCAAGACGCCGCTTACCGGCGCCACCGGTGCCTTG 
CAGGTGTGGGCCAGCTTCATGCGCAAGGCGCATCCGCAATCGCTGGAAATGCCGATGCCGGAGAACGTGGTG 
ATGGCCTGGGTCGACGCGCAGACCGGGCAGGGCTCGGCGGCGGATTGCCCGAATGCCGTACAGATGCCCTAT 
ATTCGTGGCAGCGAGCCCGCCCAGGGCCCAGGCTGCGGGAGCCAGAACCCCGCCGGCGAGGTGATGGACTGG 
GTACGAGGCTGGCTGAATTGA