ViewGene
Locus Name:
GeneID:
Location:

GeneID:2047
TrivialName: PA14_02180
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_02180
PAO1 Ortholog Locus:PA0173
Sequence Length:1050
Protein Length:349
Start: 196920
Stop: 195871
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03563.1|AE004455_4 (AE004455) probable methylesterase [Pseudomonas aeruginosa PAO1]
Identities = 348/349 (99%), Positives = 349/349 (100%)
Sequence:
ATGCCCATCAGTGTCCTGGTCGTCGACGATTCCGCCCTGATCCGCAGCCTGCTCAAGGAGATCATCCAGGCC
GACCCGGAGCTGCGCCTGGTCGGCTGTGCGCCGGACGCCTTCGTCGCCCGCGACCTGATCAAGCAGTACGCG
CCGGACGTGATCAGCCTCGACGTGGAAATGCCGCGGATGGACGGCCTGACCTTCCTCGACAAGCTGATGAAG
GCGCGGCCGACCCCGGTGCTGATGATCTCCTCGCTCACCGAGCGCGGCTCCGAAGCCACCCTGCGCGCGCTG
GAACTGGGCGCGGTGGACTTCATCGCCAAGCCCCGGCTGGGCATCGCCGAAGGCATGCAGGCCTATGCCGAG
GAAATCCGCGCCAAGCTCAAGACCGTCGCCCGCGCCCGCCTGCGCCGCCGTGCGGCGGACGCGCCGGCGCCG
CCGGAAAGCGCAGCGCCGCTGCTCAGCACCGAGAAGATCATCGCCCTCGGCGCCTCCACCGGCGGCACCGAG
GCGCTCAAGGAAGTGCTGCTCGGCCTGCCGGCGCACAGTCCCGGGGTGGTCATCACCCAGCACATGCCTCCC
GGCTTCACCCGCAGCTTCGCCGAGCGCCTGGATCGCCTGACCCGGCTCAGCGTCAGCGAAGCGCGCGACGGC
GACCGCATACTGCCCGGCCACGCCCTGGTCGCGCCGGGCGACCACCACATGGAGGTGCAGCGCTCCGGCGCC
AACTACGTGGTGCGTTTGAACCGCCAGGCGCAGGTCAACGGCCACCGCCCGGCAGTGGACGTGATGTTCGAG
TCGCTGGCTCGCTGCGCCGGGCGCAACCTGCTCGCCGGCCTGCTCACCGGCATGGGCAAGGACGGCGCGCGC
GGGCTCCTGGCGATCCGCCAGGCCGGCGGCTACACCCTGGCCCAGGACGAGGCTACCTGCGTGGTCTACGGC
ATGCCCCGCGAAGCGGTGGAACTGGGCGCCGCGGAAGACGTGCTGCCGCTCGAACGGATCGCCGCCGCGCTG
CTCCAGCAAGCCGCCCGGCGCGGCAGCGGCAACCGCCTCTGA
Translation:
MPISVLVVDDSALIRSLLKEIIQADPELRLVGCAPDAFVARDLIKQYAPDVISLDVEMPRMDGLTFLDKLMK
ARPTPVLMISSLTERGSEATLRALELGAVDFIAKPRLGIAEGMQAYAEEIRAKLKTVARARLRRRAADAPAP
PESAAPLLSTEKIIALGASTGGTEALKEVLLGLPAHSPGVVITQHMPPGFTRSFAERLDRLTRLSVSEARDG
DRILPGHALVAPGDHHMEVQRSGANYVVRLNRQAQVNGHRPAVDVMFESLARCAGRNLLAGLLTGMGKDGAR
GLLAIRQAGGYTLAQDEATCVVYGMPREAVELGAAEDVLPLERIAAALLQQAARRGSGNRL*
AnnotationID:2055GeneID:2047
AnnotatorUID: diggins
Modification Date/Time: 2005-03-23 07:07:57
Gene Name:cheB
Confidence Code:2
GeneProduct:glutamate methylesterase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(10) Chemotaxis
Alternate Gene Product Name:response regulator for chemotaxis (cheA sensor)
Functional Category Confidence Code:5
COGs:COG2201,pfam01339
Secondary Functional Category(ies):signal transduction mechanism
EC Number:3.1.1.61
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAD18554.1| (AL646084) PROBABLE PROTEIN-GLUTAMATE METHYLESTERASE [Ralstonia
           solanacearum]
          Length = 381

 Score =  412 bits (1058), Expect = e-114
 Identities = 213/375 (56%), Positives = 265/375 (70%), Gaps = 28/375 (7%)

>gb|AAL20833.1| (AE008785) methyl esterase [Salmonella typhimurium LT2]
          Length = 349

 Score =  409 bits (1051), Expect = e-113
 Identities = 200/335 (59%), Positives = 262/335 (78%)

>gb|AAO68633.1| (AE016837) glutamate methylesterase [Salmonella enterica subsp.
           enterica serovar Typhi Ty2]
          Length = 349

 Score =  409 bits (1051), Expect = e-113
 Identities = 200/335 (59%), Positives = 262/335 (78%)








 
Structural Features:
COG2201, CheB, Chemotaxis response regulator containing a CheY-like 
receiver domain and a methylesterase domain 
[Cell motility and secretion / Signal transduction mechanisms]
CD-Length = 350 residues,  99.7% aligned
Score =  401 bits (1032), Expect = 6e-113
Identities = 189/349 (54%), Positives = 241/349 (69%), Gaps = 4/349 (1%)

pfam01339, CheB_methylest, CheB methylesterase.
CD-Length = 184 residues,  99.5% aligned
Score =  229 bits (586), Expect = 3e-61
Identities = 104/183 (56%), Positives = 132/183 (72%), Gaps = 1/183 (0%)







   
Genomic Context:






   
Comment:


















    ReferenceID:4097




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21243




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33540




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2047_cdd.html


    		




 
URL:

















    ReferenceID:35486




  
Author/Investigator(s):
        Kato J, Nakamura T, Kuroda A, Ohtake H.





  
Title:
        Cloning and characterization of chemotaxis genes in Pseudomonas aeruginosa.




  
PubMed:
        10052136


MedLine:
        




    
Source:
Biosci Biotechnol Biochem. 1999 Jan;63(1):155-61.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10052136









           
Homologs By Global Alignment 

           

       



     
Gene ID:2047
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3543
                           PA0173_tr
                           translation of PA0173
                           349
                           99.71
                           99.99
                           0
                           1727.0
     

     
     
109270
                           gb|AAG03563.1|AE004455_4
                           (AE004455) probable methylesterase [Pseudomonas aeruginosa PAO1]
                           349
                           99.71
                           99.99
                           0
                           1727.0
     

     
     
109272
                           gb|AAL20833.1|
                           (AE008785) methyl esterase [Salmonella typhimurium LT2]
                           355
                           58.02
                           76.61
                           12
                           1050.0
     

     
     
109273
                           gb|AAO68633.1|
                           (AE016837) glutamate methylesterase [Salmonella enterica subsp. enterica serovar Typhi Ty2]
                           355
                           58.02
                           76.61
                           12
                           1050.0
     

     
     
109282
                           emb|CAE36778.1|
                           (BX640427) protein-glutamate methylesterase [Bordetella parapertussis]
                           357
                           57.98
                           74.78
                           15
                           1037.0
     

     






      

          
GC ORFID: 45049How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0173Glimmer Score: 

          
Start: 5998259Stop: 5997210

          
Length: 1050


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0173 translation of PA0173Homolog Bit Score 669.0

          
Other Homologs:  psyr_15may02_Scaffold15_revised_gene2551 (ORF: BLASTX 322.0), t_PA0173 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCATCAGTGTCCTGGTCGTCGACGATTCCGCCCTGATCCGCAGCCTGCTCAAGGAGATCATCCAGGCC 
GACCCGGAGCTGCGCCTGGTCGGCTGTGCGCCGGACGCCTTCGTCGCCCGCGACCTGATCAAGCAGTACGCG 
CCGGACGTGATCAGCCTCGACGTGGAAATGCCGCGGATGGACGGCCTGACCTTCCTCGACAAGCTGATGAAG 
GCGCGGCCGACCCCGGTGCTGATGATCTCCTCGCTCACCGAGCGCGGCTCCGAAGCCACCCTGCGCGCGCTG 
GAACTGGGCGCGGTGGACTTCATCGCCAAGCCCCGGCTGGGCATCGCCGAAGGCATGCAGGCCTATGCCGAG 
GAAATCCGCGCCAAGCTCAAGACCGTCGCCCGCGCCCGCCTGCGCCGCCGTGCGGCGGACGCGCCGGCGCCG 
CCGGAAAGCGCAGCGCCGCTGCTCAGCACCGAGAAGATCATCGCCCTCGGCGCCTCCACCGGCGGCACCGAG 
GCGCTCAAGGAAGTGCTGCTCGGCCTGCCGGCGCACAGTCCCGGGGTGGTCATCACCCAGCACATGCCTCCC 
GGCTTCACCCGCAGCTTCGCCGAGCGCCTGGATCGCCTGACCCGGCTCAGCGTCAGCGAAGCGCGCGACGGC 
GACCGCATACTGCCCGGCCACGCCCTGGTCGCGCCGGGCGACCACCACATGGAGGTGCAGCGCTCCGGCGCC 
AACTACGTGGTGCGTTTGAACCGCCAGGCGCAGGTCAACGGCCACCGCCCGGCAGTGGACGTGATGTTCGAG 
TCGCTGGCTCGCTGCGCCGGGCGCAACCTGCTCGCCGGCCTGCTCACCGGCATGGGCAAGGACGGCGCGCGC 
GGGCTCCTGGCGATCCGCCAGGCCGGCGGCTACACCCTGGCCCAGGACGAGGCTACCTGCGTGGTCTACGGC 
ATGCCCCGCGAAGCGGTGGAACTGGGCGCCGCGGAAGACGTGCTGCCGCTCGAACGGATCGCCGCCGCGCTG 
CTCCAGCAAGCCGCCCGGCGCGGCAGCGGCAACCGCCTCTGA