ViewGene
Locus Name:
GeneID:
Location:

GeneID:2033
TrivialName: PA14_10250
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_10250
PAO1 Ortholog Locus:PA4151
Sequence Length:1020
Protein Length:339
Start: 883278
Stop: 882259
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07538.1|AE004831_10 (AE004831) acetoin catabolism protein AcoB [Pseudomonas aeruginosa PAO1]
Identities = 338/339 (99%), Positives = 338/339 (99%)
Sequence:
ATGGCGAGAAAGATCAGCTACCAGCAGGCCATCAACGAGGCGCTGGCCCAGGAAATGCGCCGCGACCCGAGC
GTCTTCATCATGGGCGAAGACAATGCCGGCGGCGCCGGCGCGCCCGGCGAGGACGACGCCTGGGGCGGCGTG
CTGGGGGTCACCAAGGGCCTCTACCACCAGTTCCCCGGCCGCGTGCTGGATACCCCGTTGTCAGAGATCGGC
TATGTCGGCGCCGCGGTCGGCGCCGCCACCCGCGGCATGCGCCCGGTGTGCGAACTGATGTTCGTCGACTTC
GCCGGTTGCTGCCTGGACCAGATCCTCAACCAGGCCGCCAAGTTCCGCTACATGTTCGGCGGCAAGGCGGTC
ACCCCGCTGGTGATCCGTACCATGGTCGGCGCCGGCCTGCGCGCCGCCGCCCAACATTCGCAGATGCTCACT
TCGCTATGGACCCACATCCCCGGCCTCAAGGTGGTCTGCCCGTCGTCGCCCTACGACGCCAAGGGCCTGCTG
GTCCAGGCGATCCGCGACAACGACCCGGTGATCTTCTGCGAGCACAAGCTGCTCTATTCGATGCAGGGCGAG
GTACCCGAGGAGCTGTACAGCGTACCCTTCGGCGAGGCGAACTTCCTCCGCGACGGCGACGACGTCACCCTG
GTGACCTACGGCCGCATGGTCCACCTGGCGCTGGACGCCGCCGCCAGCCTGGCGCGCCAGGGCATCTCCTGC
GAAGTACTGGACCTGCGCAGCACCAGCCCGCTGGACGAGGACAGCATTCTCGAAAGCGTGGAAAAGACCGGC
CGCCTGGTGGTGGTCGACGAAGCCAACCCACGCTGCTCGATGGCCACCGACATCGCCGCGCTGGTCGCCGAA
CGGGCCTTCTCCGCGCTGCACGCGCCGATCCGCCGGGTCACCGCGCCCCATACGCCGGTGCCTTTCTCCGAC
GCCCTGGAAGACCTCTACATCCCCGACGCGGCGAAGATCGAGGCAGCCGTGCGCCAGGCCCTGGAAGGGAGG
GACGCGGCATGA
Translation:
MARKISYQQAINEALAQEMRRDPSVFIMGEDNAGGAGAPGEDDAWGGVLGVTKGLYHQFPGRVLDTPLSEIG
YVGAAVGAATRGMRPVCELMFVDFAGCCLDQILNQAAKFRYMFGGKAVTPLVIRTMVGAGLRAAAQHSQMLT
SLWTHIPGLKVVCPSSPYDAKGLLVQAIRDNDPVIFCEHKLLYSMQGEVPEELYSVPFGEANFLRDGDDVTL
VTYGRMVHLALDAAASLARQGISCEVLDLRSTSPLDEDSILESVEKTGRLVVVDEANPRCSMATDIAALVAE
RAFSALHAPIRRVTAPHTPVPFSDALEDLYIPDAAKIEAAVRQALEGRDAA*
AnnotationID:2041GeneID:2033
AnnotatorUID: diggins
Modification Date/Time: 2005-03-30 16:04:49
Gene Name:acoB
Confidence Code:2
GeneProduct:acetoin catabolism protein AcoB
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:acetoin dehydrogenase, beta subunit
Functional Category Confidence Code:5
COGs:COG0022
Secondary Functional Category(ies):energy production and conversion
EC Number:1.1.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66181.1|AE016245_10 (AE016776) acetoin dehydrogenase, beta subunit [Pseudomonas putida
           KT2440]
          Length = 340

 Score =  619 bits (1595), Expect = e-176
 Identities = 305/339 (89%), Positives = 324/339 (95%)

>gb|AAA21949.1| (M66060) acetoin:DCPIP oxidoreductase-beta [Ralstonia eutropha]
          Length = 338

 Score =  491 bits (1265), Expect = e-138
 Identities = 241/335 (71%), Positives = 281/335 (83%)

>gb|AAP26610.1| (AE017032) TPP-dependent acetoin dehydrogenase E1 beta-subunit
           [Bacillus anthracis str. Ames]
          Length = 344

 Score =  415 bits (1067), Expect = e-115
 Identities = 208/336 (61%), Positives = 256/336 (76%), Gaps = 3/336 (0%)






 
Structural Features:
COG0022, AcoB, Pyruvate/2-oxoglutarate dehydrogenase complex, 
dehydrogenase (E1) component, eukaryotic type, beta subunit 
[Energy production and conversion]
CD-Length = 324 residues, 100.0% aligned
Score =  464 bits (1197), Expect = 5e-132
Identities = 182/334 (54%), Positives = 231/334 (69%), Gaps = 12/334 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4069




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21215




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36982




  
Author/Investigator(s):
        Priefert H, Hein S, Kruger N, Zeh K, Schmidt B, Steinbuchel A.





  
Title:
        Identification and molecular characterization of the Alcaligenes eutrophus H16 aco operon genes involved in acetoin catabolism.




  
PubMed:
        2061286


MedLine:
        




    
Source:
J Bacteriol. 1991 Jul;173(13):4056-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2061286

















    ReferenceID:36985




  
Author/Investigator(s):
        Kruger N, Oppermann FB, Lorenzl H, Steinbuchel A.





  
Title:
        Biochemical and molecular characterization of the Clostridium magnum acetoin dehydrogenase enzyme system.




  
PubMed:
        8206840


MedLine:
        




    
Source:
J Bacteriol. 1994 Jun;176(12):3614-30.

Erratum in:
    * J Bacteriol 1994 Aug;176(16):5193.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8206840

















    ReferenceID:36990




  
Author/Investigator(s):
        Oppermann FB, Steinbuchel A.





  
Title:
        Identification and molecular characterization of the aco genes encoding the Pelobacter carbinolicus acetoin dehydrogenase enzyme system.




  
PubMed:
        8110297


MedLine:
        




    
Source:
J Bacteriol. 1994 Jan;176(2):469-85.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8110297

















    ReferenceID:36994




  
Author/Investigator(s):
        Huang M, Oppermann FB, Steinbuchel A.





  
Title:
        Molecular characterization of the Pseudomonas putida 2,3-butanediol catabolic pathway.




  
PubMed:
        7813883


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1994 Dec 1;124(2):141-50.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=95113288&dopt=Abstract

















    ReferenceID:38086




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type: (text/plain)







    
Data:2033_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2033
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3525
                           PA4151_tr
                           translation of PA4151
                           339
                           99.70
                           99.70
                           0
                           1732.0
     

     
     
108827
                           gb|AAG07538.1|AE004831_10
                           (AE004831) acetoin catabolism protein AcoB [Pseudomonas aeruginosa PAO1]
                           339
                           99.70
                           99.70
                           0
                           1732.0
     

     
     
108828
                           gb|AAN66181.1|AE016245_10
                           (AE016776) acetoin dehydrogenase, beta subunit [Pseudomonas putida KT2440]
                           340
                           90.29
                           95.58
                           1
                           1596.0
     

     
     
108829
                           gb|AAB58980.1|
                           (L35343) TPP-dependent acetoin dehydrogenase beta-subunit [Pseudomonas putida]
                           340
                           79.70
                           86.76
                           1
                           1382.0
     

     
     
108830
                           gb|AAA21949.1|
                           (M66060) acetoin:DCPIP oxidoreductase-beta [Ralstonia eutropha]
                           339
                           71.09
                           82.89
                           1
                           1260.0
     

     






      

          
GC ORFID: 45035How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4151Glimmer Score: 

          
Start: 4070659Stop: 4071678

          
Length: 1020


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4151 translation of PA4151Homolog Bit Score 671.0

          
Other Homologs:  t_PA3416 (ORF: BLASTX 220.0), t_PA4151 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCGAGAAAGATCAGCTACCAGCAGGCCATCAACGAGGCGCTGGCCCAGGAAATGCGCCGCGACCCGAGC 
GTCTTCATCATGGGCGAAGACAATGCCGGCGGCGCCGGCGCGCCCGGCGAGGACGACGCCTGGGGCGGCGTG 
CTGGGGGTCACCAAGGGCCTCTACCACCAGTTCCCCGGCCGCGTGCTGGATACCCCGTTGTCAGAGATCGGC 
TATGTCGGCGCCGCGGTCGGCGCCGCCACCCGCGGCATGCGCCCGGTGTGCGAACTGATGTTCGTCGACTTC 
GCCGGTTGCTGCCTGGACCAGATCCTCAACCAGGCCGCCAAGTTCCGCTACATGTTCGGCGGCAAGGCGGTC 
ACCCCGCTGGTGATCCGTACCATGGTCGGCGCCGGCCTGCGCGCCGCCGCCCAACATTCGCAGATGCTCACT 
TCGCTATGGACCCACATCCCCGGCCTCAAGGTGGTCTGCCCGTCGTCGCCCTACGACGCCAAGGGCCTGCTG 
GTCCAGGCGATCCGCGACAACGACCCGGTGATCTTCTGCGAGCACAAGCTGCTCTATTCGATGCAGGGCGAG 
GTACCCGAGGAGCTGTACAGCGTACCCTTCGGCGAGGCGAACTTCCTCCGCGACGGCGACGACGTCACCCTG 
GTGACCTACGGCCGCATGGTCCACCTGGCGCTGGACGCCGCCGCCAGCCTGGCGCGCCAGGGCATCTCCTGC 
GAAGTACTGGACCTGCGCAGCACCAGCCCGCTGGACGAGGACAGCATTCTCGAAAGCGTGGAAAAGACCGGC 
CGCCTGGTGGTGGTCGACGAAGCCAACCCACGCTGCTCGATGGCCACCGACATCGCCGCGCTGGTCGCCGAA 
CGGGCCTTCTCCGCGCTGCACGCGCCGATCCGCCGGGTCACCGCGCCCCATACGCCGGTGCCTTTCTCCGAC 
GCCCTGGAAGACCTCTACATCCCCGACGCGGCGAAGATCGAGGCAGCCGTGCGCCAGGCCCTGGAAGGGAGG 
GACGCGGCATGA