ViewGene
Locus Name:
GeneID:
Location:

GeneID:2032
TrivialName: PA14_12130
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_12130
PAO1 Ortholog Locus:PA3996
Sequence Length:984
Protein Length:327
Start: 1048270
Stop: 1049253
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07383.1|AE004817_7 (AE004817) lipoate synthase [Pseudomonas aeruginosa PAO1]
Identities = 327/327 (100%), Positives = 327/327 (100%)
Sequence:
ATGAGCACTGTTGTGGAGAAGTCCGGAGAAGCCAAGCCGGGCAAGGTGGAAGTCGGCGTGAAGCTGCGCGGC
GCGGAAAAGGTCGCGCGGATTCCGGTGAAGATCATTCCCACCGAGGAACTGCCGAAGAAACCCGACTGGATC
CGCGTGCGCATCCCGGTTTCCCCCGAGGTCGACCGCATCAAGCAACTGCTGCGCAAGCACAAGCTGCACAGC
GTCTGCGAAGAGGCGTCCTGCCCGAACCTGGGCGAGTGCTTCTCCGGCGGCACCGCGACCTTCATGATCATG
GGTGACATCTGCACCCGGCGCTGCCCGTTCTGCGACGTCGGCCATGGCCGGCCGAAGCCGCTGGACGTCGAC
GAGCCGACCAACCTGGCCATCGCCATCGCCGACCTGCGGCTGAAGTACGTGGTGATCACCTCGGTGGACCGC
GACGACCTGCGCGACGGCGGTGCCCAGCACTTCGCCGACTGCCTGCGCGAGATCCGCAAGCTGTCGCCGGGG
ATCCAGCTGGAAACCCTGGTGCCCGACTATCGCGGTCGCATGGACATCGCCCTGGAGATCACCGCCAACGAG
CCGCCGGACGTGTTCAACCATAACCTGGAGACGGTACCGCGCCTGTACCGCTCCTCGCGGCCGGGCTCGGAC
TTCGAGTGGTCGCTGGACCTGCTGCAGAAGTTCAAGCAGATGGTTCCGCACGTACCGACCAAGTCCGGGCTG
ATGCTCGGCCTGGGCGAGACCGACGATGAGGTCATCGAGGTCATGCAGCGGATGCGCGAGCACGACATCGAC
ATGCTGACCCTCGGCCAGTACCTGCAGCCGTCGCGCAACCACCTGCCGGTGCAGCGCTTCGTGCATCCGGAC
ACCTTCGCCTGGTTCGCCGAGGAAGGCGAGAAGATGGGCTTCAAGAACGTCGCGTCCGGCCCGCTGGTGCGT
TCGTCCTACCATGCCGACCAGCAGGCGCACGGCAACAAGATCGGCTGA
Translation:
MSTVVEKSGEAKPGKVEVGVKLRGAEKVARIPVKIIPTEELPKKPDWIRVRIPVSPEVDRIKQLLRKHKLHS
VCEEASCPNLGECFSGGTATFMIMGDICTRRCPFCDVGHGRPKPLDVDEPTNLAIAIADLRLKYVVITSVDR
DDLRDGGAQHFADCLREIRKLSPGIQLETLVPDYRGRMDIALEITANEPPDVFNHNLETVPRLYRSSRPGSD
FEWSLDLLQKFKQMVPHVPTKSGLMLGLGETDDEVIEVMQRMREHDIDMLTLGQYLQPSRNHLPVQRFVHPD
TFAWFAEEGEKMGFKNVASGPLVRSSYHADQQAHGNKIG*
AnnotationID:2040GeneID:2032
AnnotatorUID: danlee
Modification Date/Time: 2006-02-03 17:05:13
Gene Name:lis
Confidence Code:2
GeneProduct:Lipoate synthase
Cell Localization:(1) Cytoplasmic
Synonyms:PA3996, lipA
Cell Localization Confidence Code:3
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0320,KOG2672,smart00729
Secondary Functional Category(ies):Fatty acid metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:






 
Structural Features:
gnl|CDD|10194 COG0320, LipA, Lipoate synthase [Coenzyme metabolism]
Length = 306
Score =  497 bits (1282), Expect = e-141
Identities = 192/306 (62%), Positives = 238/306 (77%), Gaps = 2/306 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4067




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21213




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34106




  
Author/Investigator(s):
        Wohlfarth S, Hoesche C, Strunk C, Winkler UK.





  
Title:
        Molecular genetics of the extracellular lipase of Pseudomonas aeruginosa PAO1.




  
PubMed:
        1512563


MedLine:
        




    
Source:
J Gen Microbiol. 1992 Jul;138 ( Pt 7):1325-35.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1512563









           
Homologs By Global Alignment 

           

       



     
Gene ID:2032
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3524
                           PA3996_tr
                           translation of PA3996
                           327
                           99.99
                           99.99
                           0
                           1730.0
     

     
     
108715
                           gb|AAG07383.1|AE004817_7
                           (AE004817) lipoate synthase [Pseudomonas aeruginosa PAO1]
                           327
                           99.99
                           99.99
                           0
                           1730.0
     

     
     
108717
                           gb|AAO58247.1|
                           (AE016873) lipoic acid synthetase [Pseudomonas syringae pv. tomato str. DC3000]
                           331
                           87.00
                           91.23
                           17
                           1519.0
     

     
     
108716
                           gb|AAN70369.1|AE016679_1
                           (AE016792) lipoic acid synthetase [Pseudomonas putida KT2440]
                           338
                           86.98
                           92.01
                           11
                           1566.0
     

     
     
108725
                           emb|CAE30672.1|
                           (BX640437) lipoic acid synthetase [Bordetella bronchiseptica] [Bordetella bronchiseptica RB50]
                           335
                           62.98
                           75.82
                           10
                           1103.0
     

     






      

          
GC ORFID: 45034How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3996Glimmer Score: 

          
Start: 3905668Stop: 3904685

          
Length: 984


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3996 translation of PA3996Homolog Bit Score 671.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5334 (ORF: BLASTX 597.0), t_PA3996 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCACTGTTGTGGAGAAGTCCGGAGAAGCCAAGCCGGGCAAGGTGGAAGTCGGCGTGAAGCTGCGCGGC 
GCGGAAAAGGTCGCGCGGATTCCGGTGAAGATCATTCCCACCGAGGAACTGCCGAAGAAACCCGACTGGATC 
CGCGTGCGCATCCCGGTTTCCCCCGAGGTCGACCGCATCAAGCAACTGCTGCGCAAGCACAAGCTGCACAGC 
GTCTGCGAAGAGGCGTCCTGCCCGAACCTGGGCGAGTGCTTCTCCGGCGGCACCGCGACCTTCATGATCATG 
GGTGACATCTGCACCCGGCGCTGCCCGTTCTGCGACGTCGGCCATGGCCGGCCGAAGCCGCTGGACGTCGAC 
GAGCCGACCAACCTGGCCATCGCCATCGCCGACCTGCGGCTGAAGTACGTGGTGATCACCTCGGTGGACCGC 
GACGACCTGCGCGACGGCGGTGCCCAGCACTTCGCCGACTGCCTGCGCGAGATCCGCAAGCTGTCGCCGGGG 
ATCCAGCTGGAAACCCTGGTGCCCGACTATCGCGGTCGCATGGACATCGCCCTGGAGATCACCGCCAACGAG 
CCGCCGGACGTGTTCAACCATAACCTGGAGACGGTACCGCGCCTGTACCGCTCCTCGCGGCCGGGCTCGGAC 
TTCGAGTGGTCGCTGGACCTGCTGCAGAAGTTCAAGCAGATGGTTCCGCACGTACCGACCAAGTCCGGGCTG 
ATGCTCGGCCTGGGCGAGACCGACGATGAGGTCATCGAGGTCATGCAGCGGATGCGCGAGCACGACATCGAC 
ATGCTGACCCTCGGCCAGTACCTGCAGCCGTCGCGCAACCACCTGCCGGTGCAGCGCTTCGTGCATCCGGAC 
ACCTTCGCCTGGTTCGCCGAGGAAGGCGAGAAGATGGGCTTCAAGAACGTCGCGTCCGGCCCGCTGGTGCGT 
TCGTCCTACCATGCCGACCAGCAGGCGCACGGCAACAAGATCGGCTGA