ViewGene
Locus Name:
GeneID:
Location:

GeneID:2030
TrivialName: PA14_25360
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_25360
PAO1 Ortholog Locus:PA2993
Sequence Length:1029
Protein Length:342
Start: 2217682
Stop: 2218710
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06381.1|AE004724_10 (AE004724) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 337/342 (98%), Positives = 338/342 (98%)
Sequence:
GTGGCTGAGCCTTTCCGTCCGCTTGCCAAGGCCGGACTGGCAGCGCTGGCGCTGGTCCTGGCCGGTTGCGGC
GACACCCTGGAAAGCTTCGGCGGCCCGACCATGGGCAGCACCTATTCGATCAAGTACGTGCGCGGCGCCTCG
GCGCCCGACGTCCAGACGGCGAAAGCCGCGGTGGAAGCGATCCTCGCCGAGGTCGACCGGCAGATGTCCACC
TACCGCGACGACTCCCTGGTCTCGCGTTTCAATGCGCTGCCGGCGCAGTCCTGCATGGAGCTGCCGCCACCG
ATGCTGGAGTTGCTGCGCTACGGCGGCGAGTTGTCGGAACAGAGCCAGGGCGCCTTCGACATGACCGTCGAG
CCGCTGATGAACCTCTGGGGCTTCGGTCCGCAGGCGCGGGTGGAGAAGGTTCCCAGCGCCGAGCAGATCGCC
GCGGCGCGCCGTGACGTCGGCCATCGACACCTGCGCATCGACGGCCAGCGCCTGTGCAAGGACGCCGCCGTA
CAGCTGGACTTCGACAGCATCGCCGCTGGCTATACGGTGGACGCGGTAGGCGAACGCCTGAAGGAGCTTGGC
GTGCGCAGCTACCTGGCGGAAATCACCGGCGAGTTGAAAGCCGAAGGGCGCAAGCCGGACGGCACGCCCTGG
CGCATCGCTATCGAAGCGCCGCGCGAGGGCCAGCGGGTCGCCCAGCAGGTGCTGGCGCTGGACGGCTACGGC
GTCTCGACCTCGGGCGATTACCGCAACTATTTCGAAGAGAACGGCCAGCGCTATTCGCACACCTTCGACCCG
CGCACCGGCGCGCCGATCGACCATCACCTGGCTTCGGTCACGGTGCTCGATCCGTCGACGCGCAACGCCGAC
GGCCTGTCGACCCTGCTGATGGTGCTCGGTCCGGAAGAGGGCTACCGTTTCGCCGAGAAGCACCGACTGGCG
GCGCTGTTCGTCAGTCGCCAGGGCAACGGTTTCGACAGCCGCACCACGCCACGCTACGAGCAACTGTTCGGC
AACCAAGGAGAACGTCCATGA
Translation:
MAEPFRPLAKAGLAALALVLAGCGDTLESFGGPTMGSTYSIKYVRGASAPDVQTAKAAVEAILAEVDRQMST
YRDDSLVSRFNALPAQSCMELPPPMLELLRYGGELSEQSQGAFDMTVEPLMNLWGFGPQARVEKVPSAEQIA
AARRDVGHRHLRIDGQRLCKDAAVQLDFDSIAAGYTVDAVGERLKELGVRSYLAEITGELKAEGRKPDGTPW
RIAIEAPREGQRVAQQVLALDGYGVSTSGDYRNYFEENGQRYSHTFDPRTGAPIDHHLASVTVLDPSTRNAD
GLSTLLMVLGPEEGYRFAEKHRLAALFVSRQGNGFDSRTTPRYEQLFGNQGERP*
AnnotationID:2039GeneID:2030
AnnotatorUID: diggins
Modification Date/Time: 2005-04-11 13:01:50
Gene Name:
Confidence Code:3
GeneProduct:putative thiamine biosynthesis lipoprotein
Cell Localization:()
Synonyms:apbE
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam02424
Secondary Functional Category(ies):amino acid biosynthesis and metabolims
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67763.1|AE016408_1 (AE016782) thiamine biosynthesis lipoprotein, putative [Pseudomonas
           putida KT2440]
          Length = 332

 Score =  382 bits (980), Expect = e-105
 Identities = 187/331 (56%), Positives = 242/331 (73%), Gaps = 2/331 (0%)

>gb|AAO55623.1| (AE016863) thiamine biosynthesis lipoprotein, putative [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 343

 Score =  335 bits (860), Expect = 2e-91
 Identities = 171/324 (52%), Positives = 225/324 (69%), Gaps = 3/324 (0%)

>gb|AAF95433.1| (AE004300) thiamin biosynthesis lipoprotein ApbE [Vibrio cholerae]
          Length = 367

 Score =  267 bits (682), Expect = 8e-71
 Identities = 141/316 (44%), Positives = 199/316 (62%), Gaps = 5/316 (1%)






 
Structural Features:
pfam02424, ApbE, ApbE family. 
CD-Length = 314 residues,  93.3% aligned
Score =  234 bits (598), Expect = 1e-62
Identities = 110/298 (36%), Positives = 166/298 (55%), Gaps = 9/298 (3%)







   
Genomic Context:






   
Comment:


















    ReferenceID:14483




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21209




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36062




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2030_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2030
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3522
                           PA2993_tr
                           translation of PA2993
                           342
                           98.53
                           98.83
                           0
                           1732.0
     

     
     
107698
                           gb|AAG06381.1|AE004724_10
                           (AE004724) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           342
                           98.24
                           98.83
                           0
                           1729.0
     

     
     
107699
                           gb|AAN67763.1|AE016408_1
                           (AE016782) thiamine biosynthesis lipoprotein, putative [Pseudomonas putida KT2440]
                           344
                           54.36
                           70.34
                           14
                           981.0
     

     
     
107700
                           gb|AAO55623.1|
                           (AE016863) thiamine biosynthesis lipoprotein, putative [Pseudomonas syringae pv. tomato str. DC3000]
                           356
                           48.31
                           64.04
                           27
                           853.0
     

     
     
107712
                           emb|CAE41506.1|
                           (BX640414) thiamine biosynthesis lipoprotein precursor [Bordetella pertussis] [Bordetella pertussis Tohama I]
                           350
                           41.42
                           56.28
                           20
                           604.0
     

     






      

          
GC ORFID: 45032How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2993Glimmer Score: 

          
Start: 2736243Stop: 2735215

          
Length: 1029


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2993 translation of PA2993Homolog Bit Score 671.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene896 (ORF: BLASTX 337.0), t_PA2993 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGGCTGAGCCTTTCCGTCCGCTTGCCAAGGCCGGACTGGCAGCGCTGGCGCTGGTCCTGGCCGGTTGCGGC 
GACACCCTGGAAAGCTTCGGCGGCCCGACCATGGGCAGCACCTATTCGATCAAGTACGTGCGCGGCGCCTCG 
GCGCCCGACGTCCAGACGGCGAAAGCCGCGGTGGAAGCGATCCTCGCCGAGGTCGACCGGCAGATGTCCACC 
TACCGCGACGACTCCCTGGTCTCGCGTTTCAATGCGCTGCCGGCGCAGTCCTGCATGGAGCTGCCGCCACCG 
ATGCTGGAGTTGCTGCGCTACGGCGGCGAGTTGTCGGAACAGAGCCAGGGCGCCTTCGACATGACCGTCGAG 
CCGCTGATGAACCTCTGGGGCTTCGGTCCGCAGGCGCGGGTGGAGAAGGTTCCCAGCGCCGAGCAGATCGCC 
GCGGCGCGCCGTGACGTCGGCCATCGACACCTGCGCATCGACGGCCAGCGCCTGTGCAAGGACGCCGCCGTA 
CAGCTGGACTTCGACAGCATCGCCGCTGGCTATACGGTGGACGCGGTAGGCGAACGCCTGAAGGAGCTTGGC 
GTGCGCAGCTACCTGGCGGAAATCACCGGCGAGTTGAAAGCCGAAGGGCGCAAGCCGGACGGCACGCCCTGG 
CGCATCGCTATCGAAGCGCCGCGCGAGGGCCAGCGGGTCGCCCAGCAGGTGCTGGCGCTGGACGGCTACGGC 
GTCTCGACCTCGGGCGATTACCGCAACTATTTCGAAGAGAACGGCCAGCGCTATTCGCACACCTTCGACCCG 
CGCACCGGCGCGCCGATCGACCATCACCTGGCTTCGGTCACGGTGCTCGATCCGTCGACGCGCAACGCCGAC 
GGCCTGTCGACCCTGCTGATGGTGCTCGGTCCGGAAGAGGGCTACCGTTTCGCCGAGAAGCACCGACTGGCG 
GCGCTGTTCGTCAGTCGCCAGGGCAACGGTTTCGACAGCCGCACCACGCCACGCTACGAGCAACTGTTCGGC 
AACCAAGGAGAACGTCCATGA