ViewGene
Locus Name:
GeneID:
Location:

GeneID:2026
TrivialName: PA14_45110
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_45110
PAO1 Ortholog Locus:PA1493
Sequence Length:999
Protein Length:332
Start: 4023471
Stop: 4024469
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04882.1|AE004578_4 (AE004578) sulfate-binding protein of ABC transporter [Pseudomonas aeruginosa PAO1]
Identities = 332/332 (100%), Positives = 332/332 (100%)
Sequence:
GTGAAACGACTGTTCAGCGCCTCCCTGCTGGCCGCCGGCCTGGCGCTCGGCGGCGCCGCTCACGCCGCCCAG
CCCCTGCTCAACGTCTCCTACGACGTGATGCGCGACTTCTACAAGGAATACAACCCGGCCTTCCAGAAATAC
TGGAAAGCGGAGAAAGGCGAGAACATCACCATCCAGATGTCCCATGGCGGTTCCAGCAAGCAGGCGCGCTCG
GTGATCGACGGTCTGCCCGCCGACGTCATCACCATGAACCAGGCCACCGACATCGACGCCCTCGCCGACAAC
GGCGGCCTGGTGCCGAAGGACTGGGCGACCCGCCTGCCGAACAACAGCGCGCCGTTCACCTCGGCCACCGTG
TTCATCGTTCGCAAGGGCAACCCCAAGGCCCTGAAGGACTGGCCGGACCTGCTCAAGGACGGCGTCCAGGTA
GTGGTGCCGAACCCCAAGACCTCCGGCAACGGCCGCTATACCTATCTCTCCGCCTGGGGCTACGTGCTGAAG
AACGGCGGTGACGAGAACAAGGCCAAGGAGTTCGTCGGCAAACTGTTCAAGCAGGTACCGGTGCTCGACACC
GGCGGCCGCGCCGCCACCACTACCTTCATGCAGAACCAGATCGGCGACGTACTGGTGACCTTCGAGAACGAA
GCCGAGATGATCGCCCGCGAATTCGGCCGAGGCGGCTTCGAGGTGGTCTACCCGAGCGTGTCCGCCGAGGCC
GAGCCTCCGGTGGCGGTGGTCGACAAGGTGGTCGAGAAGAAAGGCAGCCGCGCCCAGGCCGAGGCCTACCTG
AAGTACCTGTGGTCGGACGAGGGCCAGACCATCGCCGCCAACAACTACCTGCGCCCGCGCAACCCGGAGATC
CTCGCCAAGTTCGCCGACCGCTTCCCGAAAGTCGACTTCTTCTCGGTGGAGAAGACCTTCGGCGACTGGCGC
AGCGTGCAGAAGACCCACTTCATCGACGGCGGCGTATTCGACCAGATCTACAGCCCCAACTGA
Translation:
MKRLFSASLLAAGLALGGAAHAAQPLLNVSYDVMRDFYKEYNPAFQKYWKAEKGENITIQMSHGGSSKQARS
VIDGLPADVITMNQATDIDALADNGGLVPKDWATRLPNNSAPFTSATVFIVRKGNPKALKDWPDLLKDGVQV
VVPNPKTSGNGRYTYLSAWGYVLKNGGDENKAKEFVGKLFKQVPVLDTGGRAATTTFMQNQIGDVLVTFENE
AEMIAREFGRGGFEVVYPSVSAEAEPPVAVVDKVVEKKGSRAQAEAYLKYLWSDEGQTIAANNYLRPRNPEI
LAKFADRFPKVDFFSVEKTFGDWRSVQKTHFIDGGVFDQIYSPN*
AnnotationID:2035GeneID:2026
AnnotatorUID: danlee
Modification Date/Time: 2006-02-03 15:03:05
Gene Name:cysP
Confidence Code:2
GeneProduct:sulfate-binding protein of ABC transporter
Cell Localization:(8) Periplasmic
Synonyms:PA1493
Cell Localization Confidence Code:2
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:periplasmic sulfate binding protein
Functional Category Confidence Code:5
COGs:COG1613,COG4150
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57097.1| (AE016869) sulfate ABC transporter, periplasmic sulfate-binding
           protein [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 332

 Score =  568 bits (1463), Expect = e-161
 Identities = 278/329 (84%), Positives = 298/329 (90%)

>gb|AAN69885.1|AE016627_7 (AE016790) periplasmic thiosulfate-binding protein [Pseudomonas
           putida KT2440]
          Length = 332

 Score =  566 bits (1458), Expect = e-161
 Identities = 277/330 (83%), Positives = 298/330 (90%)

>gb|AAC76899.1| (AE000466) periplasmic sulfate-binding protein [Escherichia coli
           K12]
          Length = 329

 Score =  357 bits (915), Expect = 7e-98
 Identities = 170/304 (55%), Positives = 223/304 (73%), Gaps = 2/304 (0%)






 
Structural Features:
COG1613, Sbp, ABC-type sulfate transport system, periplasmic component 
[Inorganic ion transport and metabolism] 
CD-Length = 348 residues,  94.3% aligned
Score =  513 bits (1322), Expect = 1e-146
Identities = 211/328 (64%), Positives = 247/328 (75%), Gaps = 1/328 (0%)

COG4150, CysP, ABC-type sulfate transport system, periplasmic component 
[Inorganic ion transport and metabolism]
CD-Length = 341 residues,  97.9% aligned
Score =  390 bits (1004), Expect = 1e-109
Identities = 171/334 (51%), Positives = 220/334 (65%), Gaps = 4/334 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14479




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21201




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37344




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2026_cdd.html


    		




 
URL:

















    ReferenceID:37945




  
Author/Investigator(s):
        Hryniewicz M, Sirko A, Palucha A, Bock A, Hulanicka D.





  
Title:
        Sulfate and thiosulfate transport in Escherichia coli K-12: identification of a gene encoding a novel protein involved in thiosulfate binding.




  
PubMed:
        2188959


MedLine:
        




    
Source:
J Bacteriol. 1990 Jun;172(6):3358-66.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2188959

















    ReferenceID:37946




  
Author/Investigator(s):
        Mansilla MC, de Mendoza D.





  
Title:
        The Bacillus subtilis cysP gene encodes a novel sulphate permease related to the inorganic phosphate transporter (Pit) family.




  
PubMed:
        10784039


MedLine:
        




    
Source:
Microbiology. 2000 Apr;146 ( Pt 4):815-21.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10784039









           
Homologs By Global Alignment 

           

       



     
Gene ID:2026
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3516
                           PA1493_tr
                           translation of PA1493
                           332
                           99.99
                           99.99
                           0
                           1732.0
     

     
     
107607
                           gb|AAG04882.1|AE004578_4
                           (AE004578) sulfate-binding protein of ABC transporter [Pseudomonas aeruginosa PAO1]
                           332
                           99.69
                           99.99
                           0
                           1729.0
     

     
     
107608
                           gb|AAO33317.1|AF226874_2
                           (AF226874) periplasmic sulfate binding protein [Pseudomonas aeruginosa]
                           332
                           98.49
                           98.79
                           4
                           1705.0
     

     
     
107609
                           gb|AAO57097.1|
                           (AE016869) sulfate ABC transporter, periplasmic sulfate-binding protein [Pseudomonas syringae pv. tomato str. DC3000]
                           332
                           83.43
                           89.75
                           0
                           1456.0
     

     
     
107610
                           gb|AAN69885.1|AE016627_7
                           (AE016790) periplasmic thiosulfate-binding protein [Pseudomonas putida KT2440]
                           332
                           83.13
                           89.75
                           0
                           1452.0
     

     






      

          
GC ORFID: 45028How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1493Glimmer Score: 

          
Start: 930438Stop: 929440

          
Length: 999


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1493 translation of PA1493Homolog Bit Score 671.0

          
Other Homologs:  t_PA0283 (ORF: BLASTX 388.0), psyr_15may02_Scaffold2_revised_gene4048 (ORF: BLASTX 350.0), t_PA1493 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGAAACGACTGTTCAGCGCCTCCCTGCTGGCCGCCGGCCTGGCGCTCGGCGGCGCCGCTCACGCCGCCCAG 
CCCCTGCTCAACGTCTCCTACGACGTGATGCGCGACTTCTACAAGGAATACAACCCGGCCTTCCAGAAATAC 
TGGAAAGCGGAGAAAGGCGAGAACATCACCATCCAGATGTCCCATGGCGGTTCCAGCAAGCAGGCGCGCTCG 
GTGATCGACGGTCTGCCCGCCGACGTCATCACCATGAACCAGGCCACCGACATCGACGCCCTCGCCGACAAC 
GGCGGCCTGGTGCCGAAGGACTGGGCGACCCGCCTGCCGAACAACAGCGCGCCGTTCACCTCGGCCACCGTG 
TTCATCGTTCGCAAGGGCAACCCCAAGGCCCTGAAGGACTGGCCGGACCTGCTCAAGGACGGCGTCCAGGTA 
GTGGTGCCGAACCCCAAGACCTCCGGCAACGGCCGCTATACCTATCTCTCCGCCTGGGGCTACGTGCTGAAG 
AACGGCGGTGACGAGAACAAGGCCAAGGAGTTCGTCGGCAAACTGTTCAAGCAGGTACCGGTGCTCGACACC 
GGCGGCCGCGCCGCCACCACTACCTTCATGCAGAACCAGATCGGCGACGTACTGGTGACCTTCGAGAACGAA 
GCCGAGATGATCGCCCGCGAATTCGGCCGAGGCGGCTTCGAGGTGGTCTACCCGAGCGTGTCCGCCGAGGCC 
GAGCCTCCGGTGGCGGTGGTCGACAAGGTGGTCGAGAAGAAAGGCAGCCGCGCCCAGGCCGAGGCCTACCTG 
AAGTACCTGTGGTCGGACGAGGGCCAGACCATCGCCGCCAACAACTACCTGCGCCCGCGCAACCCGGAGATC 
CTCGCCAAGTTCGCCGACCGCTTCCCGAAAGTCGACTTCTTCTCGGTGGAGAAGACCTTCGGCGACTGGCGC 
AGCGTGCAGAAGACCCACTTCATCGACGGCGGCGTATTCGACCAGATCTACAGCCCCAACTGA