ViewGene
Locus Name:
GeneID:
Location:

GeneID:2020
TrivialName: PA14_60670
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_60670
PAO1 Ortholog Locus:PA4585
Sequence Length:1026
Protein Length:341
Start: 5407156
Stop: 5408181
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07973.1|AE004872_4 (AE004872) RNA 3'-terminal phosphate cyclase [Pseudomonas aeruginosa PAO1]
Identities = 341/341 (100%), Positives = 341/341 (100%)
Sequence:
ATGAGAAAGGACCTCATCGAACTCGACGGCAGCGAAGGCGGCGGGCAGATCCTGCGTAGCGCCCTGAGCCTG
TCGATGACCAGCGGCCAGCCGCTGCGCATCCGCAACATCCGTGGCCGGCGCAGCCGTCCGGGGCTGCTGCGC
CAGCACCTCACCGCGGTGCGCGCGGCGGCGGAAATCTGCGCGGCCGAGGTCGAGGGTGCCGAGCTCGGCTCA
CGCGAGCTGGCGTTCCGGCCCGGTGCGATCCGCGCCGGCGACTACGCCTTCGCCATCGGCTCCGCCGGCAGT
TGCTCGCTGGTACTGCAAACCTTGCTGCCGGCGCTGCTCGCGGCCAACGGCGAAAGCCGGGTGAGGATCAGC
GGCGGCACCCACAATCCACTGGCGCCGCCGGCGGACTTTCTCCGCGACAGCTGGCTGCCGCTGTTGCAGCGG
ATGGGCGCGGAAGTCGACCTGGAACTGCTGCGCCACGGCTTCGTACCCGCCGGCGGTGGCGAGCTGCTGGCG
CGGGTACGCCCGGCGCGCTGGCGGCCGCTGCAACTGGAGCATCCCGGCGCGGCGCTGCGGCGCCAGGCGCGG
GCGCTGCTCGCAGGGATTCCCGGGCACGTCGGCGAGCGCGAGCTGGAGAGGGTCCGCCAGCGCCTCGGTTGG
AGCGACGAAGAACGCCAGCTGGAGTTCCTCGCGGAGGACCAGGGGCCGGGCAACGCCCTGCTGCTGCGGATC
GACTGCGAGCACATCTGCGCGACCTTCTGCGCCTTCGGCCAGGCCGGCGTCAGCGCCGAACGGGTGGCGGAA
CAGGTCGCGACGCAAGCCATCGGCTGGATGGAAAGCGGCTGCGCGGCGGACGAGCACCTCGCCGACCAATTG
CTGCTGCCCATGGCCCTGGCCGGCGCCGGCAGCTTCACCACGCCACGGCTGAGCGCCCATCTGCAAAGCAAC
CGCCGGGTTATCGAACGCTTCCTGCCGGTGAGGATCGGCGACCAGGCACTGGACGGCGGCGGTCATCGCATC
GTCATCACATCGGCTTGA
Translation:
MRKDLIELDGSEGGGQILRSALSLSMTSGQPLRIRNIRGRRSRPGLLRQHLTAVRAAAEICAAEVEGAELGS
RELAFRPGAIRAGDYAFAIGSAGSCSLVLQTLLPALLAANGESRVRISGGTHNPLAPPADFLRDSWLPLLQR
MGAEVDLELLRHGFVPAGGGELLARVRPARWRPLQLEHPGAALRRQARALLAGIPGHVGERELERVRQRLGW
SDEERQLEFLAEDQGPGNALLLRIDCEHICATFCAFGQAGVSAERVAEQVATQAIGWMESGCAADEHLADQL
LLPMALAGAGSFTTPRLSAHLQSNRRVIERFLPVRIGDQALDGGGHRIVITSA*
AnnotationID:2029GeneID:2020
AnnotatorUID: diggins
Modification Date/Time: 2005-05-06 05:05:46
Gene Name:rtcA
Confidence Code:2
GeneProduct:RNA 3'-terminal phosphate cyclase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(24) Transcription, RNA processing and degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd00874,pfam01137,cd00295
Secondary Functional Category(ies):
EC Number:6.5.1.4
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN82635.1|AE016768_53 (AE016768) RNA 3'-terminal phosphate cyclase [Escherichia coli
           CFT073]
          Length = 339

 Score =  335 bits (858), Expect = 3e-91
 Identities = 186/334 (55%), Positives = 224/334 (67%), Gaps = 5/334 (1%)

>gb|AAN44902.1|AE015352_1 (AE015352) Chain B, Crystal Structure Of Rna 3'-Terminal Phosphate
           Cyclase, An Ubiquitous Enzyme With Unusual Topology
           [Shigella flexneri 2a str. 301]
          Length = 339

 Score =  333 bits (854), Expect = 9e-91
 Identities = 186/334 (55%), Positives = 225/334 (67%), Gaps = 5/334 (1%)






 
Structural Features:
cd00874, RNA_Cyclase_Class_II, RNA 3' phosphate cyclase 
domain (class II).
CD-Length = 326 residues,  98.8% aligned
Score =  401 bits (1031), Expect = 8e-113

pfam01137, RTC, RNA 3'-terminal phosphate cyclase.
CD-Length = 331 residues,  98.8% aligned
Score =  352 bits (906), Expect = 2e-98
Identities = 154/327 (47%), Positives = 198/327 (60%), Gaps = 10/327 (3%)

cd00295, RNA_Cyclase, RNA 3' phosphate cyclase domain
CD-Length = 338 residues,  96.7% aligned
Score =  241 bits (615), Expect = 1e-64






   
Genomic Context:






   
Comment:


















    ReferenceID:4043




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21189




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33117




  
Author/Investigator(s):
        Genschik P, Drabikowski K, Filipowicz W.





  
Title:
        Characterization of the Escherichia coli RNA 3'-terminal phosphate cyclase and its sigma54-regulated operon.




  
PubMed:
        9738023


MedLine:
        




    
Source:
J Biol Chem. 1998 Sep 25;273(39):25516-26.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9738023









           
Homologs By Global Alignment 

           

       



     
Gene ID:2020
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3510
                           PA4585_tr
                           translation of PA4585
                           341
                           99.99
                           99.99
                           0
                           1736.0
     

     
     
107522
                           gb|AAG07973.1|AE004872_4
                           (AE004872) RNA 3'-terminal phosphate cyclase [Pseudomonas aeruginosa PAO1]
                           341
                           99.99
                           99.99
                           0
                           1736.0
     

     
     
107525
                           gb|AAL22380.1|
                           (AE008862) RNA 3'-terminal phosphate cyclase (with b3419) [Salmonella typhimurium LT2]
                           344
                           54.06
                           64.82
                           8
                           847.0
     

     
     
107524
                           gb|AAN44902.1|AE015352_1
                           (AE015352) Chain B, Crystal Structure Of Rna 3'-Terminal Phosphate Cyclase, An Ubiquitous Enzyme With Unusual Topology [Shigella flexneri 2a str. 301]
                           348
                           54.02
                           65.51
                           16
                           856.0
     

     
     
107523
                           gb|AAN82635.1|AE016768_53
                           (AE016768) RNA 3'-terminal phosphate cyclase [Escherichia coli CFT073]
                           347
                           53.89
                           65.12
                           14
                           859.0
     

     






      

          
GC ORFID: 45022How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4585Glimmer Score: 

          
Start: 4670836Stop: 4671861

          
Length: 1026


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4585 translation of PA4585Homolog Bit Score 673.0

          
Other Homologs:  t_PA4585 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGAAAGGACCTCATCGAACTCGACGGCAGCGAAGGCGGCGGGCAGATCCTGCGTAGCGCCCTGAGCCTG 
TCGATGACCAGCGGCCAGCCGCTGCGCATCCGCAACATCCGTGGCCGGCGCAGCCGTCCGGGGCTGCTGCGC 
CAGCACCTCACCGCGGTGCGCGCGGCGGCGGAAATCTGCGCGGCCGAGGTCGAGGGTGCCGAGCTCGGCTCA 
CGCGAGCTGGCGTTCCGGCCCGGTGCGATCCGCGCCGGCGACTACGCCTTCGCCATCGGCTCCGCCGGCAGT 
TGCTCGCTGGTACTGCAAACCTTGCTGCCGGCGCTGCTCGCGGCCAACGGCGAAAGCCGGGTGAGGATCAGC 
GGCGGCACCCACAATCCACTGGCGCCGCCGGCGGACTTTCTCCGCGACAGCTGGCTGCCGCTGTTGCAGCGG 
ATGGGCGCGGAAGTCGACCTGGAACTGCTGCGCCACGGCTTCGTACCCGCCGGCGGTGGCGAGCTGCTGGCG 
CGGGTACGCCCGGCGCGCTGGCGGCCGCTGCAACTGGAGCATCCCGGCGCGGCGCTGCGGCGCCAGGCGCGG 
GCGCTGCTCGCAGGGATTCCCGGGCACGTCGGCGAGCGCGAGCTGGAGAGGGTCCGCCAGCGCCTCGGTTGG 
AGCGACGAAGAACGCCAGCTGGAGTTCCTCGCGGAGGACCAGGGGCCGGGCAACGCCCTGCTGCTGCGGATC 
GACTGCGAGCACATCTGCGCGACCTTCTGCGCCTTCGGCCAGGCCGGCGTCAGCGCCGAACGGGTGGCGGAA 
CAGGTCGCGACGCAAGCCATCGGCTGGATGGAAAGCGGCTGCGCGGCGGACGAGCACCTCGCCGACCAATTG 
CTGCTGCCCATGGCCCTGGCCGGCGCCGGCAGCTTCACCACGCCACGGCTGAGCGCCCATCTGCAAAGCAAC 
CGCCGGGTTATCGAACGCTTCCTGCCGGTGAGGATCGGCGACCAGGCACTGGACGGCGGCGGTCATCGCATC 
GTCATCACATCGGCTTGA