ViewGene
Locus Name:
GeneID:
Location:

GeneID:2013
TrivialName: PA14_35570
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_35570
PAO1 Ortholog Locus:PA2244
Sequence Length:1002
Protein Length:333
Start: 3161766
Stop: 3160765
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05632.1|AE004650_3 (AE004650) hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 331/333 (99%), Positives = 332/333 (99%)
Sequence:
ATGAGCGCAGCGCCACCGGAGATCCCCGCCCTGCCCGCCGGCCTCTGCTACGTCGACGACCGCCAGCCGGGC
ATCCGTCGACGCCGGCAGGGCAAGCGCTTCGTCTATTTCGACGCCGACGGCCAGCGCATCGGCGACGCCGAG
GAAATCCGCCGGCTGGACAAGCTGGCCATTCCGCCGGCCTATCGCGAGGTCTGGATCTGTCCCGACCCCAAC
GGCCATCTCCAGGCCACCGGCCGCGACGCCCGTGGGCGCAAGCAGTACCGCTACCACCCGCGCTGGCGCGAG
GTACGCGATGCCGACAAGTACGAGCGCCTGCTGCGCTTCGGCCAGGCGCTGCCGGGGCTGCGCCGGCACATC
GACGCCCAGTTGCGCCTGCCCGGCCTCGGTCGCGACAAGGTGGTGGCCCTGGCGGTGGCGCTGCTCGACGCC
ACCCTGATCCGCGTCGGCAACCACCGCTACGCCCGCGACAACCGCTCCTACGGCCTGACCACCCTGCGTACC
CGGCACGTCGACGTGAACGGCAGTCGCATCCGGTTCCGCTTCAAGGGCAAGAGCGGCATCGAGCACGACGTC
AGCCTGGAGCATCCGCGCCTGGCGCGGGTGCTGCGGCGCTGCCTGGAACTGCCCGGCCAGGACCTGTTGCAG
TACCTGGACGAGGACGGCCAGCCGCACCGCATCGGCTCCCACGACATCAACGAGTACCTGCGCCGGCACACC
GGCGAGGATTTCAGCGCCAAGGACTACCGCACCTGGGCCGGCAGCGCGCTGGCGCTGGAGCGCCTGCGGCGT
GCCGAAGCCGACGGGCTCGGCGCGGTGGTGGCGGAGGTCGCCGCCGAATTGGGCAACAGCGTGGCGATCTGC
CGGCAATGCTACATCCACCCGGCGATCATCGAGGCCTACCAGGCCGGGCAACTGGGCCAGCTCAGGCGCGCC
CGCAAGCGCCGCTGGCTGAGCGCCGAGGAAGCGACCCTGCTGGCCTTCCTCGACACGCCCGGCTGA
Translation:
MSAAPPEIPALPAGLCYVDDRQPGIRRRRQGKRFVYFDADGQRIGDAEEIRRLDKLAIPPAYREVWICPDPN
GHLQATGRDARGRKQYRYHPRWREVRDADKYERLLRFGQALPGLRRHIDAQLRLPGLGRDKVVALAVALLDA
TLIRVGNHRYARDNRSYGLTTLRTRHVDVNGSRIRFRFKGKSGIEHDVSLEHPRLARVLRRCLELPGQDLLQ
YLDEDGQPHRIGSHDINEYLRRHTGEDFSAKDYRTWAGSALALERLRRAEADGLGAVVAEVAAELGNSVAIC
RQCYIHPAIIEAYQAGQLGQLRRARKRRWLSAEEATLLAFLDTPG*
AnnotationID:2022GeneID:2013
AnnotatorUID: danlee
Modification Date/Time: 2005-10-10 15:03:43
Gene Name:pslN
Confidence Code:4
GeneProduct:putative DNA topoisomerase
Cell Localization:()
Synonyms:PA2244
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG3569, cd00659
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
gi|68345078|gb|AAY92684.1|  DNA topoisomerase I, putative [Pseudomonas fluorescens Pf-5]
     Length=345
     Score =  441 bits (1134),  Expect = 2e-122
     Identities = 218/330 (66%), Positives = 258/330 (78%), Gaps = 7/330 (2%)

gi|52006475|ref|ZP_00333855.1|  COG3569: Topoisomerase IB [Thiobacillus denitrificans ATCC 25259]
     Length=356
     Score =  409 bits (1050),  Expect = 9e-113
     Identities = 205/327 (62%), Positives = 245/327 (74%), Gaps = 7/327 (2%)








 
Structural Features:
COG3569, Topoisomerase IB [DNA replication, recombination, and repair]
CD-Length = 354 residues,  97.2% aligned
Score =  414 bits (1066), Expect = 6e-117
Identities = 197/344 (57%), Positives = 232/344 (67%), Gaps = 11/344 (3%)

cd00659, Topo_IB_C, DNA topoisomerase IB, C-terminal catalytic domain. 
CD-Length = 218 residues, 100.0% aligned
Score =  233 bits (596), Expect = 2e-62







   
Genomic Context:






   
Comment:


















    ReferenceID:4029




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21175




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32530




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2013_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2013
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3503
                           PA2244_tr
                           translation of PA2244
                           333
                           99.39
                           99.69
                           0
                           1739.0
     

     
     
107450
                           gb|AAG05632.1|AE004650_3
                           (AE004650) hypothetical protein PA2244 [Pseudomonas aeruginosa PAO1]
                           333
                           99.39
                           99.69
                           0
                           1739.0
     

     
     
107451
                           gb|AAN69425.1|AE016578_11
                           (AE016788) conserved hypothetical protein [Pseudomonas putida KT2440]
                           345
                           57.97
                           71.30
                           17
                           1037.0
     

     
     
107453
                           emb|CAE37961.1|
                           (BX640431) Conserved hypothetical protein [Bordetella parapertussis]
                           348
                           54.31
                           65.22
                           22
                           899.0
     

     
     
107452
                           gb|AAO56468.1|
                           (AE016866) DNA topoisomerase, type I, putative [Pseudomonas syringae pv. tomato str. DC3000]
                           356
                           53.93
                           70.50
                           28
                           1021.0
     

     






      

          
GC ORFID: 45015How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2244Glimmer Score: 

          
Start: 1792150Stop: 1793151

          
Length: 1002


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2244 translation of PA2244Homolog Bit Score 674.0

          
Other Homologs:  psyr_15may02_Scaffold7_revised_gene7036 (ORF: BLASTX 398.0), t_PA2244 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGCAGCGCCACCGGAGATCCCCGCCCTGCCCGCCGGCCTCTGCTACGTCGACGACCGCCAGCCGGGC 
ATCCGTCGACGCCGGCAGGGCAAGCGCTTCGTCTATTTCGACGCCGACGGCCAGCGCATCGGCGACGCCGAG 
GAAATCCGCCGGCTGGACAAGCTGGCCATTCCGCCGGCCTATCGCGAGGTCTGGATCTGTCCCGACCCCAAC 
GGCCATCTCCAGGCCACCGGCCGCGACGCCCGTGGGCGCAAGCAGTACCGCTACCACCCGCGCTGGCGCGAG 
GTACGCGATGCCGACAAGTACGAGCGCCTGCTGCGCTTCGGCCAGGCGCTGCCGGGGCTGCGCCGGCACATC 
GACGCCCAGTTGCGCCTGCCCGGCCTCGGTCGCGACAAGGTGGTGGCCCTGGCGGTGGCGCTGCTCGACGCC 
ACCCTGATCCGCGTCGGCAACCACCGCTACGCCCGCGACAACCGCTCCTACGGCCTGACCACCCTGCGTACC 
CGGCACGTCGACGTGAACGGCAGTCGCATCCGGTTCCGCTTCAAGGGCAAGAGCGGCATCGAGCACGACGTC 
AGCCTGGAGCATCCGCGCCTGGCGCGGGTGCTGCGGCGCTGCCTGGAACTGCCCGGCCAGGACCTGTTGCAG 
TACCTGGACGAGGACGGCCAGCCGCACCGCATCGGCTCCCACGACATCAACGAGTACCTGCGCCGGCACACC 
GGCGAGGATTTCAGCGCCAAGGACTACCGCACCTGGGCCGGCAGCGCGCTGGCGCTGGAGCGCCTGCGGCGT 
GCCGAAGCCGACGGGCTCGGCGCGGTGGTGGCGGAGGTCGCCGCCGAATTGGGCAACAGCGTGGCGATCTGC 
CGGCAATGCTACATCCACCCGGCGATCATCGAGGCCTACCAGGCCGGGCAACTGGGCCAGCTCAGGCGCGCC 
CGCAAGCGCCGCTGGCTGAGCGCCGAGGAAGCGACCCTGCTGGCCTTCCTCGACACGCCCGGCTGA