ViewGene
Locus Name:
GeneID:
Location:

GeneID:200
TrivialName: PA14_28710
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_28710
PAO1 Ortholog Locus:PA2739
Sequence Length:2379
Protein Length:792
Start: 2471477
Stop: 2473855
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06127.1|AE004702_4 (AE004702) phenylalanyl-tRNA synthetase, beta subunit [Pseudomonas aeruginosa PAO1]
Identities = 788/792 (99%), Positives = 788/792 (99%)
Sequence:
ATGAAATTCAGTGAAAAGTGGCTGCGGAGCTGGGCAAATCCGCAGGTTTCCCATGACGAGCTGGTCGCTCGC
CTGTCCATGGTCGGCCTCGAGGTCGACGCCGACCTGCCGGTCGCCGGCGCCTTCAGCGGCGTGGTGGTCGGC
GAGGTGCTCTCGACCGAACAGCATCCGGACGCCGACAAGCTGCGCGTCTGCCAGGTCAGCAACGGCAGCGAG
ACCTTCCAGGTCGTCTGCGGCGCGCCCAACGTGCGCGCGGGCCTGAAGATTCCCTTCGCCATGATCGGCGCC
GAACTGCCGGGCGACTTCAAGATCAAGAAAGCCAAGCTGCGTGGCGTCGAGTCCTTCGGCATGCTCTGCTCG
GCCAAGGAATTGCAGATCAGCGAAGAGAACGCCGGTCTGCTCGAGCTGCCGGCTGACGCGCCGGTCGGCCAG
GACGTCCGCACCTACCTGGAGCTGGACGACTACACCATCGAGGTCGGCCTGACCCCGAACCGCGGTGACTGC
CTGTCGCTCGCCGGCCTGGCCCGCGAAGTCAGCGCCATCTACGACGTACCGCTGGCGCCGGTGGcggtGGAT
GCGgTCGCCGCGCAGCACGACGAGACCCGTCCGGTCGAACTCGACGCGCCGGCCGCCTGCCCGCGCTATCTC
GGCCGGGTGATCCGCAACGTCGACCTGAGCCGGCCGACCCCGTTGTGGATGGTCGAGCGCCTGCGCCGTTCC
GACATTCGCAGTATCGACCCGGTGGTCGACGTCACCAACTACGTGATGATCGAACTGGGCCAGCCGATGCAT
GCCTTCGACCTTGCCGAGATCAACGGCGGCGTGCGCGTGCGCATGGCCGAGGACGGCGAGAAGCTGGTCCTG
CTCGACGGCCAGGAAATCACCCTGCGCGCCGACACCCTGGTGATCGCCGACCACCAGCGCGCCCTGGCCATC
GCCGGGGTGATGGGTGGTGAGCACAGCGGCGTCAGCGACAGCACCCGCGACCTGTTCCTCGAAGCCGCGTTC
TTCGACACCATCGCCCTGGCCGGCAAGGCTCGCTCCTATGGCCTGCACACCGACTCCTCGCATCGCTTCGAG
CGCGGCGTCGACAGCCAGCTGGCGCGCAAGGCCATGGAGCGCGCGACGCGCCTGATCCTCGACATCGTCGGC
GGCGAGCCGGGGCCGATCGTCGAGCAGGTCAGCGAAGCGCACCTGCCGAAGGTAGCGCCGATCACCCTGCGC
GCCGAGCGCGTGACCCAGATGCTCGGCATGCCGCTGGACGCCGCCGAGATCGTGCGCCTGCTGCAAGCCCTG
GAGCTGACGGTGGTAGCCGACGGGGAAGGGCAGTGGTCGGTCGGCGTACCGAGCCATCGCTTCGACATTTCC
CTGGAAGTCGACCTGATCGAGGAACTGGCCCGTCTCTACGGCTACAACCGCTTGCCGGTGCGCTACCCGCAG
GCGCGCCTGGCGCCGAACAACAAGCCGGAAGCGCGCGCCGCGCTGCCGCTGCTGCGGCGCCTGCTGGTCGCC
CGCGGCTACCAGGAAGCGATCACCTTCAGCTTCATCGATCCGGCCCTGTTCGAACTGTTCGATCCGGGCACC
CAGCCGCTGACCCTGGCCAACCCGATCTCCGCCGACATGGCGGCGATGCGTTCCAGTCTCTGGCCGGGCCTG
GTCAAGGCGCTGCAGCACAACCTCAACCGCCAGCAGTCGCGGGTCCGCCTGTTCGAAAGCGGCCTGCGCTTC
GTCGGTCAACTGGAAAGCTTGAAGCAGGAAGCCATGCTCGCCGGCGCCATCTGCGGCAAGCGCCTGCCGGAA
GGCTGGGCCAACGGCCGTGACGGCGTGGACTTCTTCGACGCCAAGGCCGATGTCGAGGCAGTGTTGGCCAGT
GCCGGCGCCCTCGGCGACTTCAGCTTCGTGCCCGGTGAGCACCCGGCGCTGCACCCGGGGCAGACCGCGCGC
ATCGAGCGGGAAGGGCGGCTGGTCGGCTACCTGGGCGCGCTGCATCCGGAACTGGCGAAGAAACTCGACCTC
GAGCAGCCGGTGTTCCTCTTCGAGCTGCTGTTGGCCGAAGTGGTCGACGGCCACCTGCCGAAGTTCCGCGAG
CTGTCGCGCTTCCCCGAAGTACGCCGCGACCTGGCGCTGCTGGTGGACCAGGACGTGCCGGCGCAAGACATC
CTGACACAAATCCGTGCGGCGGCCGGCGAATGGCTGACGGACCTCAGGCTGTTTGATGTGTATCACGGTAAA
GGCATTGATCCGCATAGAAAAAGCCTTGCCGTCGGCTTGACCTGGCAGCATCCATCGCGCACTCTTAATGAC
GATGAAGTCAATAGCACGACGCAGAACATCGTCACGTCGTTGGAAGAAAGGTTCAATGCCACGTTAAGGAAA
TAG
Translation:
MKFSEKWLRSWANPQVSHDELVARLSMVGLEVDADLPVAGAFSGVVVGEVLSTEQHPDADKLRVCQVSNGSE
TFQVVCGAPNVRAGLKIPFAMIGAELPGDFKIKKAKLRGVESFGMLCSAKELQISEENAGLLELPADAPVGQ
DVRTYLELDDYTIEVGLTPNRGDCLSLAGLAREVSAIYDVPLAPVAVDAVAAQHDETRPVELDAPAACPRYL
GRVIRNVDLSRPTPLWMVERLRRSDIRSIDPVVDVTNYVMIELGQPMHAFDLAEINGGVRVRMAEDGEKLVL
LDGQEITLRADTLVIADHQRALAIAGVMGGEHSGVSDSTRDLFLEAAFFDTIALAGKARSYGLHTDSSHRFE
RGVDSQLARKAMERATRLILDIVGGEPGPIVEQVSEAHLPKVAPITLRAERVTQMLGMPLDAAEIVRLLQAL
ELTVVADGEGQWSVGVPSHRFDISLEVDLIEELARLYGYNRLPVRYPQARLAPNNKPEARAALPLLRRLLVA
RGYQEAITFSFIDPALFELFDPGTQPLTLANPISADMAAMRSSLWPGLVKALQHNLNRQQSRVRLFESGLRF
VGQLESLKQEAMLAGAICGKRLPEGWANGRDGVDFFDAKADVEAVLASAGALGDFSFVPGEHPALHPGQTAR
IEREGRLVGYLGALHPELAKKLDLEQPVFLFELLLAEVVDGHLPKFRELSRFPEVRRDLALLVDQDVPAQDI
LTQIRAAAGEWLTDLRLFDVYHGKGIDPHRKSLAVGLTWQHPSRTLNDDEVNSTTQNIVTSLEERFNATLRK
*
AnnotationID:210GeneID:200
AnnotatorUID: diggins
Modification Date/Time: 2005-04-05 07:07:05
Gene Name:pheT
Confidence Code:2
GeneProduct:phenylalanyl-tRNA synthetase, beta subunit
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0072
Secondary Functional Category(ies):
EC Number:6.1.1.20
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN68082.1|AE016440_2 (AE016783) phenylalanyl-tRNA synthetase, beta subunit [Pseudomonas
           putida KT2440]
          Length = 793

 Score = 1284 bits (3322), Expect = 0.0
 Identities = 634/793 (79%), Positives = 704/793 (88%), Gaps = 1/793 (0%)

>gb|AAO55894.1| (AE016864) phenylalanyl-tRNA synthetase, beta subunit [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 792

 Score = 1256 bits (3250), Expect = 0.0
 Identities = 629/792 (79%), Positives = 691/792 (87%)

>gb|AAP16998.1| (AE016983) phenylalanine tRNA synthetase, beta-subunit [Shigella
           flexneri 2a str. 2457T]
          Length = 795

 Score =  824 bits (2129), Expect = 0.0
 Identities = 413/795 (51%), Positives = 559/795 (70%), Gaps = 5/795 (0%)






 
Structural Features:
COG0072, PheT, Phenylalanyl-tRNA synthetase beta subunit 
[Translation, ribosomal structure and biogenesis]
CD-Length = 650 residues, 100.0% aligned
Score =  695 bits (1794), Expect = 0.0
Identities = 297/655 (45%), Positives = 414/655 (63%), Gaps = 10/655 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:403




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17545




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36289




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:200_cdd.html


    		




 
URL:

















    ReferenceID:36370




  
Author/Investigator(s):
        Keller B, Kast P, Hennecke H.





  
Title:
        Cloning and sequence analysis of the phenylalanyl-tRNA synthetase genes (pheST) from Thermus thermophilus.




  
PubMed:
        1451792


MedLine:
        




    
Source:
FEBS Lett. 1992 Apr 13;301(1):83-8.

Erratum in:
    * FEBS Lett. 1992 Sep 28;310(2):204.







    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1451792

















    ReferenceID:36371




  
Author/Investigator(s):
        Brakhage AA, Wozny M, Putzer H.





  
Title:
        Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase genes.




  
PubMed:
        2127701


MedLine:
        




    
Source:
Biochimie. 1990 Oct;72(10):725-34.

Erratum in:
    * Biochimie. 1991 Jan;73(1):127.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2127701

















    ReferenceID:36372




  
Author/Investigator(s):
        Mechulam Y, Fayat G, Blanquet S.





  
Title:
        Sequence of the Escherichia coli pheST operon and identification of the himA gene.




  
PubMed:
        2991205


MedLine:
        




    
Source:
J Bacteriol. 1985 Aug;163(2):787-91.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2991205

















    ReferenceID:36373




  
Author/Investigator(s):
        Savopoulos JW, Hibbs M, Jones EJ, Mensah L, Richardson C, Fosberry A, Downes R, Fox SG, Brown JR, Jenkins O.





  
Title:
        Identification, cloning, and expression of a functional phenylalanyl-tRNA synthetase (pheRS) from Staphylococcus aureus.




  
PubMed:
        11281723


MedLine:
        




    
Source:
Protein Expr Purif. 2001 Apr;21(3):470-84.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11281723

















    ReferenceID:36378




  
Author/Investigator(s):
        Fayat G, Mayaux JF, Sacerdot C, Fromant M, Springer M, Grunberg-Manago M, Blanquet S.





  
Title:
        Escherichia coli phenylalanyl-tRNA synthetase operon region. Evidence for an attenuation mechanism. Identification of the gene for the ribosomal protein L20.




  
PubMed:
        6317865


MedLine:
        




    
Source:
J Mol Biol. 1983 Dec 15;171(3):239-61.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6317865









           
Homologs By Global Alignment 

           

       



     
Gene ID:200
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
342
                           PA2739_tr
                           translation of PA2739
                           792
                           99.49
                           99.49
                           0
                           4004.0
     

     
     
21659
                           gb|AAG06127.1|AE004702_4
                           (AE004702) phenylalanyl-tRNA synthetase, beta subunit [Pseudomonas aeruginosa PAO1]
                           792
                           99.49
                           99.49
                           0
                           4004.0
     

     
     
21660
                           gb|AAN68082.1|AE016440_2
                           (AE016783) phenylalanyl-tRNA synthetase, beta subunit [Pseudomonas putida KT2440]
                           793
                           79.94
                           88.77
                           1
                           3324.0
     

     
     
21661
                           gb|AAO55894.1|
                           (AE016864) phenylalanyl-tRNA synthetase, beta subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           792
                           79.41
                           87.24
                           0
                           3250.0
     

     
     
21680
                           dbj|BAC59554.1|
                           (AP005077) phenylalanyl-tRNA synthetase, beta chain [Vibrio parahaemolyticus]
                           803
                           52.42
                           66.62
                           13
                           2017.0
     

     






      

          
GC ORFID: 43202How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2739Glimmer Score: 

          
Start: 2482449Stop: 2480071

          
Length: 2379


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA2739 translation of PA2739Homolog Bit Score 1546.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1168 (ORF: BLASTX 1262.0), t_PA2739 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAATTCAGTGAAAAGTGGCTGCGGAGCTGGGCAAATCCGCAGGTTTCCCATGACGAGCTGGTCGCTCGC 
CTGTCCATGGTCGGCCTCGAGGTCGACGCCGACCTGCCGGTCGCCGGCGCCTTCAGCGGCGTGGTGGTCGGC 
GAGGTGCTCTCGACCGAACAGCATCCGGACGCCGACAAGCTGCGCGTCTGCCAGGTCAGCAACGGCAGCGAG 
ACCTTCCAGGTCGTCTGCGGCGCGCCCAACGTGCGCGCGGGCCTGAAGATTCCCTTCGCCATGATCGGCGCC 
GAACTGCCGGGCGACTTCAAGATCAAGAAAGCCAAGCTGCGTGGCGTCGAGTCCTTCGGCATGCTCTGCTCG 
GCCAAGGAATTGCAGATCAGCGAAGAGAACGCCGGTCTGCTCGAGCTGCCGGCTGACGCGCCGGTCGGCCAG 
GACGTCCGCACCTACCTGGAGCTGGACGACTACACCATCGAGGTCGGCCTGACCCCGAACCGCGGTGACTGC 
CTGTCGCTCGCCGGCCTGGCCCGCGAAGTCAGCGCCATCTACGACGTACCGCTGGCGCCGGTGGcggtGGAT 
GCGgTCGCCGCGCAGCACGACGAGACCCGTCCGGTCGAACTCGACGCGCCGGCCGCCTGCCCGCGCTATCTC 
GGCCGGGTGATCCGCAACGTCGACCTGAGCCGGCCGACCCCGTTGTGGATGGTCGAGCGCCTGCGCCGTTCC 
GACATTCGCAGTATCGACCCGGTGGTCGACGTCACCAACTACGTGATGATCGAACTGGGCCAGCCGATGCAT 
GCCTTCGACCTTGCCGAGATCAACGGCGGCGTGCGCGTGCGCATGGCCGAGGACGGCGAGAAGCTGGTCCTG 
CTCGACGGCCAGGAAATCACCCTGCGCGCCGACACCCTGGTGATCGCCGACCACCAGCGCGCCCTGGCCATC 
GCCGGGGTGATGGGTGGTGAGCACAGCGGCGTCAGCGACAGCACCCGCGACCTGTTCCTCGAAGCCGCGTTC 
TTCGACACCATCGCCCTGGCCGGCAAGGCTCGCTCCTATGGCCTGCACACCGACTCCTCGCATCGCTTCGAG 
CGCGGCGTCGACAGCCAGCTGGCGCGCAAGGCCATGGAGCGCGCGACGCGCCTGATCCTCGACATCGTCGGC 
GGCGAGCCGGGGCCGATCGTCGAGCAGGTCAGCGAAGCGCACCTGCCGAAGGTAGCGCCGATCACCCTGCGC 
GCCGAGCGCGTGACCCAGATGCTCGGCATGCCGCTGGACGCCGCCGAGATCGTGCGCCTGCTGCAAGCCCTG 
GAGCTGACGGTGGTAGCCGACGGGGAAGGGCAGTGGTCGGTCGGCGTACCGAGCCATCGCTTCGACATTTCC 
CTGGAAGTCGACCTGATCGAGGAACTGGCCCGTCTCTACGGCTACAACCGCTTGCCGGTGCGCTACCCGCAG 
GCGCGCCTGGCGCCGAACAACAAGCCGGAAGCGCGCGCCGCGCTGCCGCTGCTGCGGCGCCTGCTGGTCGCC 
CGCGGCTACCAGGAAGCGATCACCTTCAGCTTCATCGATCCGGCCCTGTTCGAACTGTTCGATCCGGGCACC 
CAGCCGCTGACCCTGGCCAACCCGATCTCCGCCGACATGGCGGCGATGCGTTCCAGTCTCTGGCCGGGCCTG 
GTCAAGGCGCTGCAGCACAACCTCAACCGCCAGCAGTCGCGGGTCCGCCTGTTCGAAAGCGGCCTGCGCTTC 
GTCGGTCAACTGGAAAGCTTGAAGCAGGAAGCCATGCTCGCCGGCGCCATCTGCGGCAAGCGCCTGCCGGAA 
GGCTGGGCCAACGGCCGTGACGGCGTGGACTTCTTCGACGCCAAGGCCGATGTCGAGGCAGTGTTGGCCAGT 
GCCGGCGCCCTCGGCGACTTCAGCTTCGTGCCCGGTGAGCACCCGGCGCTGCACCCGGGGCAGACCGCGCGC 
ATCGAGCGGGAAGGGCGGCTGGTCGGCTACCTGGGCGCGCTGCATCCGGAACTGGCGAAGAAACTCGACCTC 
GAGCAGCCGGTGTTCCTCTTCGAGCTGCTGTTGGCCGAAGTGGTCGACGGCCACCTGCCGAAGTTCCGCGAG 
CTGTCGCGCTTCCCCGAAGTACGCCGCGACCTGGCGCTGCTGGTGGACCAGGACGTGCCGGCGCAAGACATC 
CTGACACAAATCCGTGCGGCGGCCGGCGAATGGCTGACGGACCTCAGGCTGTTTGATGTGTATCACGGTAAA 
GGCATTGATCCGCATAGAAAAAGCCTTGCCGTCGGCTTGACCTGGCAGCATCCATCGCGCACTCTTAATGAC 
GATGAAGTCAATAGCACGACGCAGAACATCGTCACGTCGTTGGAAGAAAGGTTCAATGCCACGTTAAGGAAA 
TAG