ViewGene
Locus Name:
GeneID:
Location:

GeneID:1986
TrivialName: PA14_25510
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_25510
PAO1 Ortholog Locus:PA2981
Sequence Length:999
Protein Length:332
Start: 2229651
Stop: 2230649
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06369.1|AE004723_13 (AE004723) tetraacyldisaccharide 4*-kinase [Pseudomonas aeruginosa PAO1]
Identities = 332/332 (100%), Positives = 332/332 (100%)
Sequence:
ATGTCGTTCTCCGAGCGACTGCTCGCCGCCTGGTACCAGGGGCACCCGGCGCTGGCGCTGCTGCGTCCGCTG
GAGGCTCTCTATCGTCGGGTGGCGAACGGCCGCCGGGCGGACTTCCTGTCCGGGCGCAAGCCCGCCTACCGG
GCACCGCTGCCGGTGCTGGTGGTAGGCAATATCACCGTCGGCGGAACCGGCAAGACGCCGATGATCCTCTGG
ATGATCGAGCACTGCAGGGCTCGCGGCTTGCGGGTTGGCGTGATCAGCCGCGGCTATGGTGCCCGGCCGCCC
ACCACGCCGTGGCGGGTGCGGGCCGAGCAGGACGCCGCGGAGGCCGGCGACGAACCGCTGATGATCGTCCGG
CGCAGCGGCGTGCCGCTGATGATCGACCCGGATCGTCCGCGCGCCCTGCAGGCGCTGCTCGCCGAAGAGCAG
TTGGACCTGGTCCTCTGCGACGATGGCTTGCAGCACTATCGCCTGGCGCGCGATCTGGAGCTGGTGCTGATC
GATGCCGCGCGCGGTCTCGGCAATGGTCGTTGCCTGCCGGCCGGTCCGTTGCGCGAACCGGCGGAGCGTCTG
GAAAGCGTCGACGCGCTGCTTTACAACGGCGCCGACGAGGATCCTGACGGCGGTTACGCATTCCGCTTGCAG
CCTACTGCGCTGATCAACCTGCAGAGCGGCGAGCGTCGACCGCTGGAGCATTTTCCCGCGGGCCAGGAGGTC
CATGCCCTGGCCGGGATCGGCAATCCGCAGCGTTTCTTCAGGACGCTCGAGGCGCTACACTGGCGGGCGATT
CCGCATGCCTTCCCGGATCACGCGACCTACACGGCGGCCGAACTGGCGTTCAGCCCGCCGCTGCCGCTGCTG
ATGACCGAGAAGGATGCCGTTAAATGCCGGGCTTTCGCAGCGGCCGACTGGTGGTACCTGGCGGTCGATGCG
GTGCCTTCCCCAGCATTCGTCGCCTGGTTCGACGCCCGACTCGAGCATCTCCTGGCGCGCTGA
Translation:
MSFSERLLAAWYQGHPALALLRPLEALYRRVANGRRADFLSGRKPAYRAPLPVLVVGNITVGGTGKTPMILW
MIEHCRARGLRVGVISRGYGARPPTTPWRVRAEQDAAEAGDEPLMIVRRSGVPLMIDPDRPRALQALLAEEQ
LDLVLCDDGLQHYRLARDLELVLIDAARGLGNGRCLPAGPLREPAERLESVDALLYNGADEDPDGGYAFRLQ
PTALINLQSGERRPLEHFPAGQEVHALAGIGNPQRFFRTLEALHWRAIPHAFPDHATYTAAELAFSPPLPLL
MTEKDAVKCRAFAAADWWYLAVDAVPSPAFVAWFDARLEHLLAR*
AnnotationID:1995GeneID:1986
AnnotatorUID: diggins
Modification Date/Time: 2005-04-11 12:12:19
Gene Name:lpxK
Confidence Code:2
GeneProduct:tetraacyldisaccharide 4*-kinase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1663,pfam02606
Secondary Functional Category(ies):
EC Number:2.7.1.130
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57312.1| (AE016869) tetraacyldisaccharide 4'-kinase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 331

 Score =  524 bits (1349), Expect = e-148
 Identities = 248/329 (75%), Positives = 285/329 (86%)

>gb|AAN67519.1|AE016380_7 (AE016781) tetraacyldisaccharide 4`-kinase [Pseudomonas putida
           KT2440]
          Length = 334

 Score =  523 bits (1348), Expect = e-148
 Identities = 245/332 (73%), Positives = 289/332 (87%)

>gb|AAN55823.1|AE015719_5 (AE015719) tetraacyldisaccharide 4'-kinase [Shewanella oneidensis
           MR-1]
          Length = 335

 Score =  268 bits (684), Expect = 4e-71
 Identities = 153/324 (47%), Positives = 202/324 (62%), Gaps = 9/324 (2%)








 
Structural Features:
COG1663, LpxK, Tetraacyldisaccharide-1-P 4'-kinase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 336 residues,  99.7% aligned
Score =  348 bits (895), Expect = 4e-97
Identities = 157/340 (46%), Positives = 197/340 (57%), Gaps = 15/340 (4%)

pfam02606, LpxK, Tetraacyldisaccharide-1-P 4'-kinase.
CD-Length = 328 residues,  92.1% aligned
Score =  242 bits (619), Expect = 5e-65
Identities = 126/307 (41%), Positives = 176/307 (57%), Gaps = 18/307 (5%)







   
Genomic Context:






   
Comment:


















    ReferenceID:3975




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21121




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36073




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1986_cdd.html


    		




 
URL:

















    ReferenceID:36451




  
Author/Investigator(s):
        Garrett TA, Kadrmas JL, Raetz CR.





  
Title:
        Identification of the gene encoding the Escherichia coli lipid A 4'-kinase. Facile phosphorylation of endotoxin analogs with recombinant LpxK.




  
PubMed:
        9268317


MedLine:
        




    
Source:
J Biol Chem. 1997 Aug 29;272(35):21855-64.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9268317









           
Homologs By Global Alignment 

           

       



     
Gene ID:1986
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3457
                           PA2981_tr
                           translation of PA2981
                           332
                           99.99
                           99.99
                           0
                           1749.0
     

     
     
105936
                           gb|AAG06369.1|AE004723_13
                           (AE004723) tetraacyldisaccharide 4*-kinase [Pseudomonas aeruginosa PAO1]
                           332
                           99.99
                           99.99
                           0
                           1749.0
     

     
     
105937
                           gb|AAO57312.1|
                           (AE016869) tetraacyldisaccharide 4'-kinase [Pseudomonas syringae pv. tomato str. DC3000]
                           332
                           74.69
                           85.84
                           1
                           1346.0
     

     
     
105938
                           gb|AAN67519.1|AE016380_7
                           (AE016781) tetraacyldisaccharide 4`-kinase [Pseudomonas putida KT2440]
                           334
                           73.35
                           86.52
                           2
                           1348.0
     

     
     
105940
                           gb|AAN55823.1|AE015719_5
                           (AE015719) tetraacyldisaccharide 4'-kinase [Shewanella oneidensis MR-1]
                           347
                           44.38
                           59.07
                           27
                           690.0
     

     






      

          
GC ORFID: 44988How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2981Glimmer Score: 

          
Start: 2724274Stop: 2723276

          
Length: 999


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2981 translation of PA2981Homolog Bit Score 678.0

          
Other Homologs:  psyr_15may02_Scaffold13_revised_gene2177 (ORF: BLASTX 526.0), t_PA2981 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCGTTCTCCGAGCGACTGCTCGCCGCCTGGTACCAGGGGCACCCGGCGCTGGCGCTGCTGCGTCCGCTG 
GAGGCTCTCTATCGTCGGGTGGCGAACGGCCGCCGGGCGGACTTCCTGTCCGGGCGCAAGCCCGCCTACCGG 
GCACCGCTGCCGGTGCTGGTGGTAGGCAATATCACCGTCGGCGGAACCGGCAAGACGCCGATGATCCTCTGG 
ATGATCGAGCACTGCAGGGCTCGCGGCTTGCGGGTTGGCGTGATCAGCCGCGGCTATGGTGCCCGGCCGCCC 
ACCACGCCGTGGCGGGTGCGGGCCGAGCAGGACGCCGCGGAGGCCGGCGACGAACCGCTGATGATCGTCCGG 
CGCAGCGGCGTGCCGCTGATGATCGACCCGGATCGTCCGCGCGCCCTGCAGGCGCTGCTCGCCGAAGAGCAG 
TTGGACCTGGTCCTCTGCGACGATGGCTTGCAGCACTATCGCCTGGCGCGCGATCTGGAGCTGGTGCTGATC 
GATGCCGCGCGCGGTCTCGGCAATGGTCGTTGCCTGCCGGCCGGTCCGTTGCGCGAACCGGCGGAGCGTCTG 
GAAAGCGTCGACGCGCTGCTTTACAACGGCGCCGACGAGGATCCTGACGGCGGTTACGCATTCCGCTTGCAG 
CCTACTGCGCTGATCAACCTGCAGAGCGGCGAGCGTCGACCGCTGGAGCATTTTCCCGCGGGCCAGGAGGTC 
CATGCCCTGGCCGGGATCGGCAATCCGCAGCGTTTCTTCAGGACGCTCGAGGCGCTACACTGGCGGGCGATT 
CCGCATGCCTTCCCGGATCACGCGACCTACACGGCGGCCGAACTGGCGTTCAGCCCGCCGCTGCCGCTGCTG 
ATGACCGAGAAGGATGCCGTTAAATGCCGGGCTTTCGCAGCGGCCGACTGGTGGTACCTGGCGGTCGATGCG 
GTGCCTTCCCCAGCATTCGTCGCCTGGTTCGACGCCCGACTCGAGCATCTCCTGGCGCGCTGA