ViewGene
Locus Name:
GeneID:
Location:

GeneID:1983
TrivialName: PA14_01960
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_01960
PAO1 Ortholog Locus:PA0157
Sequence Length:1071
Protein Length:356
Start: 177315
Stop: 178385
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03547.1|AE004453_10 (AE004453) probable RND efflux membrane fusion protein precursor [Pseudomonas aeruginosa PAO1]
Identities = 356/356 (100%), Positives = 356/356 (100%)
Sequence:
ATGAAGCCGTTTTCCCTCGCCGGCCTGTTCGGCTTCGCCCTGCTCCTCTCCGGCTGCGGCGACGAGCCGCCG
CCGGCACCGCCGCGGCCGGTGCTGACGGTGACCGTGAAGACCCTGAAGAACGACGACCTCGGTCGCTTCGCC
GGGAGCATCCAGGCGCGCTACGAGAGCGTGCTCGGCTTCCGCACCAACGGACGGATCGCCTCGCGCCTGTTC
GACGTCGGTGACTTCGTCGGCAAGGGCGCGCTGCTGGCAACCCTCGACCCCACCGACCAGCAGAACCAGTTG
CGCGCCAGCCAGGGCGACCTGGCCAGCGCCGAGGCGCAGTTGATCGACGCCCAGGCCAATGCCCGGCGCCAG
GAAGAACTGTTCGCCCGCAGCGTCACCGCCCAGGCGCGCCTGGACGATGCGCGGACCCGCCTGAAGACCAGC
CAGGCCAGCTTCGACCAGGCCAAAGCGGCGGTGCAGCAGGCCAGGGACCAGCTTTCCTACACGCGCCTGGTG
ACCGATTTCGACGGCGTCATCACCACCTGGCACGCCGAGGCCGGGCAAGTGGTCAGCGCCGGCCAGGCGGTG
GTCACCCTGGCCCGGCCCGAGGTGCGCGAGGCGGTCTTCGACCTGCCCACCGAGGTCGCCGAGAGCCTGCCG
GCCGACGCGCGCTTCCTGGTCAGCGCCCAGCTCGACCCGCAGGCCAGGACCACCGGCAGCATCCGCGAGCTG
GGTCCGCAGGCCGACGCCTCGACCCGCACCCGTCGCGTGCGCCTGAGCCTGGCGCAGACGCCGGAGGCGTTC
CGCCTCGGCTCGACCATCCAGGTCCAGCTGAGCAGCGCCGGTAGCGTGCGCAGTGTGCTGCCGGCCAGCGTG
CTGCTGGAGCGGGACGGCAAGACCCAGGTCTGGGTCGTCGATGGGAAACAGTCCAGCGTGGCCCTGCGCGAG
GTACAGGTGCTCAGCCGCGACGAACGCCAGGTGGTGATCGGACAGGGCCTGGCCGACGGCGACCGGGTGGTC
CGCGCCGGAGTCAACAGCCTCAAGCCCGGCCAGAAGATCAAACTCGACGAGGATGCGCGATGA
Translation:
MKPFSLAGLFGFALLLSGCGDEPPPAPPRPVLTVTVKTLKNDDLGRFAGSIQARYESVLGFRTNGRIASRLF
DVGDFVGKGALLATLDPTDQQNQLRASQGDLASAEAQLIDAQANARRQEELFARSVTAQARLDDARTRLKTS
QASFDQAKAAVQQARDQLSYTRLVTDFDGVITTWHAEAGQVVSAGQAVVTLARPEVREAVFDLPTEVAESLP
ADARFLVSAQLDPQARTTGSIRELGPQADASTRTRRVRLSLAQTPEAFRLGSTIQVQLSSAGSVRSVLPASV
LLERDGKTQVWVVDGKQSSVALREVQVLSRDERQVVIGQGLADGDRVVRAGVNSLKPGQKIKLDEDAR*
AnnotationID:1992GeneID:1983
AnnotatorUID: diggins
Modification Date/Time: 2005-03-23 07:07:07
Gene Name:
Confidence Code:3
GeneProduct:putative RND efflux membrane fusion protein precursor
Cell Localization:()
Synonyms:PA0157
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0845
Secondary Functional Category(ies):membrane proteins
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58618.1| (AE016874) efflux transporter, RND family, MFP subunit [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 356

 Score =  390 bits (1002), Expect = e-108
 Identities = 207/343 (60%), Positives = 253/343 (73%), Gaps = 1/343 (0%)

>gb|AAN70739.1|AE016717_7 (AE016793) efflux membrane fusion protein, RND family [Pseudomonas
           putida KT2440]
          Length = 355

 Score =  388 bits (996), Expect = e-107
 Identities = 209/345 (60%), Positives = 253/345 (73%), Gaps = 1/345 (0%)








 
Structural Features:
COG0845, AcrA, Membrane-fusion protein [Cell envelope biogenesis, outer membrane]
CD-Length = 372 residues,  95.2% aligned
Score =  104 bits (260), Expect = 1e-23
Identities = 98/356 (27%), Positives = 159/356 (44%), Gaps = 10/356 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3969




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21115




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33529




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1983_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1983
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3453
                           PA0157_tr
                           translation of PA0157
                           356
                           99.99
                           99.99
                           0
                           1752.0
     

     
     
105893
                           gb|AAG03547.1|AE004453_10
                           (AE004453) probable RND efflux membrane fusion protein precursor [Pseudomonas aeruginosa PAO1]
                           356
                           99.99
                           99.99
                           0
                           1752.0
     

     
     
105895
                           gb|AAN70739.1|AE016717_7
                           (AE016793) efflux membrane fusion protein, RND family [Pseudomonas putida KT2440]
                           357
                           59.38
                           71.98
                           3
                           997.0
     

     
     
105894
                           gb|AAO58618.1|
                           (AE016874) efflux transporter, RND family, MFP subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           358
                           59.21
                           72.06
                           4
                           1004.0
     

     
     
105896
                           gb|AAO56779.1|
                           (AE016867) efflux transporter, RND family, MFP subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           374
                           45.72
                           61.22
                           26
                           776.0
     

     






      

          
GC ORFID: 44985How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0157Glimmer Score: 

          
Start: 5978654Stop: 5979724

          
Length: 1071


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0157 translation of PA0157Homolog Bit Score 679.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3258 (ORF: BLASTX 400.0), psyr_15may02_Scaffold11_revised_gene1932 (ORF: BLASTX 281.0), t_PA0157 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGCCGTTTTCCCTCGCCGGCCTGTTCGGCTTCGCCCTGCTCCTCTCCGGCTGCGGCGACGAGCCGCCG 
CCGGCACCGCCGCGGCCGGTGCTGACGGTGACCGTGAAGACCCTGAAGAACGACGACCTCGGTCGCTTCGCC 
GGGAGCATCCAGGCGCGCTACGAGAGCGTGCTCGGCTTCCGCACCAACGGACGGATCGCCTCGCGCCTGTTC 
GACGTCGGTGACTTCGTCGGCAAGGGCGCGCTGCTGGCAACCCTCGACCCCACCGACCAGCAGAACCAGTTG 
CGCGCCAGCCAGGGCGACCTGGCCAGCGCCGAGGCGCAGTTGATCGACGCCCAGGCCAATGCCCGGCGCCAG 
GAAGAACTGTTCGCCCGCAGCGTCACCGCCCAGGCGCGCCTGGACGATGCGCGGACCCGCCTGAAGACCAGC 
CAGGCCAGCTTCGACCAGGCCAAAGCGGCGGTGCAGCAGGCCAGGGACCAGCTTTCCTACACGCGCCTGGTG 
ACCGATTTCGACGGCGTCATCACCACCTGGCACGCCGAGGCCGGGCAAGTGGTCAGCGCCGGCCAGGCGGTG 
GTCACCCTGGCCCGGCCCGAGGTGCGCGAGGCGGTCTTCGACCTGCCCACCGAGGTCGCCGAGAGCCTGCCG 
GCCGACGCGCGCTTCCTGGTCAGCGCCCAGCTCGACCCGCAGGCCAGGACCACCGGCAGCATCCGCGAGCTG 
GGTCCGCAGGCCGACGCCTCGACCCGCACCCGTCGCGTGCGCCTGAGCCTGGCGCAGACGCCGGAGGCGTTC 
CGCCTCGGCTCGACCATCCAGGTCCAGCTGAGCAGCGCCGGTAGCGTGCGCAGTGTGCTGCCGGCCAGCGTG 
CTGCTGGAGCGGGACGGCAAGACCCAGGTCTGGGTCGTCGATGGGAAACAGTCCAGCGTGGCCCTGCGCGAG 
GTACAGGTGCTCAGCCGCGACGAACGCCAGGTGGTGATCGGACAGGGCCTGGCCGACGGCGACCGGGTGGTC 
CGCGCCGGAGTCAACAGCCTCAAGCCCGGCCAGAAGATCAAACTCGACGAGGATGCGCGATGA