ViewGene
Locus Name:
GeneID:
Location:

GeneID:198
TrivialName: PA14_36710
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_36710
PAO1 Ortholog Locus:PA2153
Sequence Length:2199
Protein Length:732
Start: 3271984
Stop: 3269786
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05541.1|AE004642_8 (AE004642) 1,4-alpha-glucan branching enzyme [Pseudomonas aeruginosa PAO1]
Identities = 728/732 (99%), Positives = 731/732 (99%)
Sequence:
ATGAGCGAACCGATGTCCGAACGCGAACGCTGGCAACTGGAGCTGGACAAGCTGCAGCGCGGCCTGCAGGGC
GATCCATTCGCCTTCCTCGGCCCGCAGCGCGACCCCGGCGGCGAAGGCGGCGTGCTGCGCGCCTACCTGCCC
GGCGCGCAGCGCGTCGAGCTGCTCGACGAAGACGGCGCGACGCTCGCCGAACTGGAGCAGAGCGACCCCGGC
AGCGGCTTGTTCCAGCGCCGCCTGGAGCGCCTGCCGCCGCGCTACCGCCTGCGCGTGCACTGGCCGGACGGC
GTGCAGGAAAGCGAAGACCCCTATGCCTTCGGGCCGTTGCTCGGCGAACTCGACCTTTACCTGTTCGCCGAA
GGCAACCACCGCCAACTGGCCTCCTGCCTGGGCGCGCAACTGACCCGTCACGAAGGCGTGGAGGGCGTGCGC
TTCGCGGTCTGGGCGCCGAACGCCGTGCGCGTCTCGGTGGTCGGCGATTTCAACGGCTGGGACGGGCGCCGT
CACCCGATGCGACGACGCTATCCCTCCGGCGTCTGGGAACTGTTCGTCCCGCGCCTCGGCGAGGGCGAACTG
TACAAGTACGAGTTGCAGGGCCACGACGGCCTCCTGCCGCTGAAGGCCGACCCCCTGGCGCTGGCCTGCGAG
ACGCCGCCCGGAACCGCTTCGAAGACCTGCGCGGCGTTGCGCCACGAGTGGCGCGACCAGGACTGGCTGGCC
CGGCGCGCGGAGCGCCAGGGCTATGCGGCACCGCTGTCGATCTACGAAGTCCACGCCGGCTCCTGGCGCCAC
CGCGACGGCCGGCCGCCGCACTGGTCGGAACTGGCGGAGGAGCTGATTCCCTACGTGCGCCAGCTCGGCTTC
ACCCATATCGAGCTGATGCCGGTGATGGAACATCCCTTCGGCGGCTCCTGGGGCTACCAGCCGCTCGGGTTG
TTCGCCCCCACCGCGCGCTACGGCACGCCGGAGGACTTCGCCGCGTTCGTCGACGCCTGCCACCAGGCCGGC
ATCGGCGTGATCCTCGACTGGGTGCCGGCGCATTTTCCCACCGACGCCCACGGCCTGGGCCGTTTCGACGGC
ACGGCGCTGTACGAGTACGAACATCCCTTCGAAGGCTTCCACCAGGACTGGGATACCTACATCTACAACCTT
GGCCGCAGCGAGGTGCACGGCTTCATGCTGGCCTCGGCGCTGCACTGGCTGCGCACCTACCACGTCGACGGC
CTGCGGGTGGATGCGGTGGCCTCGATGCTCTACCGCGACTATTCGCGCAAGGAAGGCGAATGGCTGCCGAAC
CGCCATGGCGGCCGGGAGAACCTGGAGGCCATCGACTTCCTCCATCACCTCAACCAGGTGGTCGCCAGCGAG
ACCCCCGGCGCGCTGGTGATCGCCGAGGAATCCACCGCCTGGCCCGGCGTCAGCCGTCCGGTGGCGGAGGGC
GGCCTGGGCTTCAGCCATAAGTGGAACATGGGCTGGATGCACGACAGCCTGGCGTACATCGGCGAAGACCCG
CTGCACCGGCGCTATCACCACCACCAGTTGACCTTCGGCCTGCTCTACGCCTTCTCCGAGCATTTCATCCTG
CCGATCTCCCACGACGAGGTGGTACATGGCAAGCACTCGCTACTGGACAAGATGCCCGGCGACCGCTGGCAG
AAGTTCGCCAACCTGCGCCTGTACCTGAGCTTCATGTGGTCGCACCCGGGCAAGAAGCTGCTGTTCATGGGC
TGCGAGTTCGGCCAGTGGCGCGAGTGGAACCACGACGGCGAACTGGACTGGTACCTGCTGCGCTATCCGGAG
CATCAGGGCGTACAGGATCTGGTGGCGGCCCTCAACCGGCTGTACCGCGATCTCCCCGCACTGCATGCGCGC
GACGGCGAGGCGCTCGGCTTCGAATGGCTGATCGGCGACGACCAGGCGAACAGTGTCTACGCCTGGCTGCGC
CACGCGGCCGGCGAGCCATCGCTGCTGGCGGTACACAATTTCACCCCGGTGCCGCGCCAGGGCTATCGGATC
GGCGTACCGCAAGGCGGCGACTGGGACGTGCTGCTCAATAGCGACGCCCAGGCGTTCGCCGGTTCCGGCTCA
GGCAGCCAGGGCCGCGTCTCCAGCGAGTCGTGCGGCGCCCATGGCCAGGCGCAGTCGCTGCTGCTCGACCTG
CCGCCGCTGGGCACGCTGCTGCTGCGTCCGGCCGGCTGA
Translation:
MSEPMSERERWQLELDKLQRGLQGDPFAFLGPQRDPGGEGGVLRAYLPGAQRVELLDEDGATLAELEQSDPG
SGLFQRRLERLPPRYRLRVHWPDGVQESEDPYAFGPLLGELDLYLFAEGNHRQLASCLGAQLTRHEGVEGVR
FAVWAPNAVRVSVVGDFNGWDGRRHPMRRRYPSGVWELFVPRLGEGELYKYELQGHDGLLPLKADPLALACE
TPPGTASKTCAALRHEWRDQDWLARRAERQGYAAPLSIYEVHAGSWRHRDGRPPHWSELAEELIPYVRQLGF
THIELMPVMEHPFGGSWGYQPLGLFAPTARYGTPEDFAAFVDACHQAGIGVILDWVPAHFPTDAHGLGRFDG
TALYEYEHPFEGFHQDWDTYIYNLGRSEVHGFMLASALHWLRTYHVDGLRVDAVASMLYRDYSRKEGEWLPN
RHGGRENLEAIDFLHHLNQVVASETPGALVIAEESTAWPGVSRPVAEGGLGFSHKWNMGWMHDSLAYIGEDP
LHRRYHHHQLTFGLLYAFSEHFILPISHDEVVHGKHSLLDKMPGDRWQKFANLRLYLSFMWSHPGKKLLFMG
CEFGQWREWNHDGELDWYLLRYPEHQGVQDLVAALNRLYRDLPALHARDGEALGFEWLIGDDQANSVYAWLR
HAAGEPSLLAVHNFTPVPRQGYRIGVPQGGDWDVLLNSDAQAFAGSGSGSQGRVSSESCGAHGQAQSLLLDL
PPLGTLLLRPAG*
AnnotationID:208GeneID:198
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 18:06:41
Gene Name:glgB
Confidence Code:2
GeneProduct:1,4-alpha-glucan branching enzyme
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0296
Secondary Functional Category(ies):carbohydrate transport and metabolism
EC Number:2.4.1.18
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69648.1|AE016599_3 (AE016789) 1,4-alpha-glucan branching enzyme [Pseudomonas putida
           KT2440]
          Length = 736

 Score = 1068 bits (2763), Expect = 0.0
 Identities = 497/727 (68%), Positives = 587/727 (80%), Gaps = 10/727 (1%)

>gb|AAM35048.1| (AE011639) 1,4-alpha-glucan branching enzyme [Xanthomonas
           axonopodis pv. citri str. 306]
          Length = 724

 Score =  915 bits (2365), Expect = 0.0
 Identities = 446/717 (62%), Positives = 521/717 (72%), Gaps = 7/717 (0%)

>emb|CAE33356.1| (BX640445) 1,4-alpha-glucan branching enzyme [Bordetella
           bronchiseptica]
          Length = 731

 Score =  854 bits (2207), Expect = 0.0
 Identities = 421/716 (58%), Positives = 492/716 (68%), Gaps = 11/716 (1%)






 
Structural Features:
COG0296, GlgB, 1,4-alpha-glucan branching enzyme 
[Carbohydrate transport and metabolism]
CD-Length = 628 residues, 100.0% aligned
Score =  794 bits (2051), Expect = 0.0
Identities = 373/633 (58%), Positives = 440/633 (69%), Gaps = 12/633 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:399




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17541




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32575




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:198_cdd.html


    		




 
URL:

















    ReferenceID:34430




  
Author/Investigator(s):
        Lim WJ, Park SR, Kim MK, An CL, Yun HJ, Hong SY, Kim EJ, Shin EC, Lee SW, Lim YP, Yun HD.





  
Title:
        Cloning and characterization of the glycogen branching enzyme gene existing in tandem with the glycogen debranching enzyme from Pectobacterium chrysanthemi PY35.




  
PubMed:
        12480526


MedLine:
        




    
Source:
Biochem Biophys Res Commun. 2003 Jan 3;300(1):93-101.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12480526

















    ReferenceID:34431




  
Author/Investigator(s):
        Homerova D, Kormanec J.





  
Title:
        Cloning of the putative glycogen branching enzyme gene, glgB, from Streptomyces aureofaciens.




  
PubMed:
        8068720


MedLine:
        




    
Source:
Biochim Biophys Acta. 1994 Aug 18;1200(3):334-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8068720

















    ReferenceID:34432




  
Author/Investigator(s):
        Kiel JA, Boels JM, Beldman G, Venema G.





  
Title:
        The glgB gene from the thermophile Bacillus caldolyticus encodes a thermolabile branching enzyme.




  
PubMed:
        1296817


MedLine:
        




    
Source:
DNA Seq. 1992;3(4):221-32.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1296817

















    ReferenceID:34433




  
Author/Investigator(s):
        Rumbak E, Rawlings DE, Lindsey GG, Woods DR.





  
Title:
        Characterization of the Butyrivibrio fibrisolvens glgB gene, which encodes a glycogen-branching enzyme with starch-clearing activity.




  
PubMed:
        1938880


MedLine:
        




    
Source:
J Bacteriol. 1991 Nov;173(21):6732-41.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1938880

















    ReferenceID:34434




  
Author/Investigator(s):
        Kiel JA, Boels JM, Beldman G, Venema G.





  
Title:
        Molecular cloning and nucleotide sequence of the glycogen branching enzyme gene (glgB) from Bacillus stearothermophilus and expression in Escherichia coli and Bacillus subtilis.




  
PubMed:
        1745226


MedLine:
        




    
Source:
Mol Gen Genet. 1991 Nov;230(1-2):136-44.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1745226

















    ReferenceID:34435




  
Author/Investigator(s):
        Kiel JA, Boels JM, Beldman G, Venema G.





  
Title:
        Nucleotide sequence of the Synechococcus sp. PCC7942 branching enzyme gene (glgB): expression in Bacillus subtilis.




  
PubMed:
        2142668


MedLine:
        




    
Source:
Gene. 1990 Apr 30;89(1):77-84.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2142668

















    ReferenceID:34436




  
Author/Investigator(s):
        Takata H, Takaha T, Okada S, Takagi M, Imanaka T.





  
Title:
        Characterization of a gene cluster for glycogen biosynthesis and a heterotetrameric ADP-glucose pyrophosphorylase from Bacillus stearothermophilus.




  
PubMed:
        9244254


MedLine:
        




    
Source:
 Bacteriol. 1997 Aug;179(15):4689-98.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9244254

















    ReferenceID:34437




  
Author/Investigator(s):
        Ugalde JE, Lepek V, Uttaro A, Estrella J, Iglesias A, Ugalde RA.





  
Title:
        Gene organization and transcription analysis of the Agrobacterium tumefaciens glycogen (glg) operon: two transcripts for the single phosphoglucomutase gene.




  
PubMed:
        9851999


MedLine:
        




    
Source:
J Bacteriol. 1998 Dec;180(24):6557-64.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9851999

















    ReferenceID:34438




  
Author/Investigator(s):
        Igarashi RY, Meyer CR.





  
Title:
        Cloning and sequencing of glycogen metabolism genes from Rhodobacter sphaeroides 2.4.1. Expression and characterization of recombinant ADP-glucose pyrophosphorylase.




  
PubMed:
        10729189


MedLine:
        




    
Source:
Arch Biochem Biophys. 2000 Apr 1;376(1):47-58.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10729189

















    ReferenceID:34439




  
Author/Investigator(s):
        Lim WJ, Park SR, Kim MK, An CL, Yun HJ, Hong SY, Kim EJ, Shin EC, Lee SW, Lim YP, Yun HD.





  
Title:
        Cloning and characterization of the glycogen branching enzyme gene existing in tandem with the glycogen debranching enzyme from Pectobacterium chrysanthemi PY35.




  
PubMed:
        12480526


MedLine:
        




    
Source:
Biochem Biophys Res Commun. 2003 Jan 3;300(1):93-101.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12480526









           
Homologs By Global Alignment 

           

       



     
Gene ID:198
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
339
                           PA2153_tr
                           translation of PA2153
                           732
                           99.45
                           99.86
                           0
                           4012.0
     

     
     
21433
                           gb|AAG05541.1|AE004642_8
                           (AE004642) 1,4-alpha-glucan branching enzyme [Pseudomonas aeruginosa PAO1]
                           732
                           99.45
                           99.86
                           0
                           4012.0
     

     
     
21434
                           gb|AAN69648.1|AE016599_3
                           (AE016789) 1,4-alpha-glucan branching enzyme [Pseudomonas putida KT2440]
                           745
                           67.65
                           80.00
                           22
                           2775.0
     

     
     
21435
                           gb|AAO56262.1|
                           (AE016865) 1,4-alpha-glucan branching enzyme [Pseudomonas syringae pv. tomato str. DC3000]
                           752
                           67.02
                           77.52
                           31
                           2705.0
     

     
     
21436
                           gb|AAM35048.1|
                           (AE011639) 1,4-alpha-glucan branching enzyme [Xanthomonas axonopodis pv. citri str. 306]
                           737
                           61.19
                           71.64
                           18
                           2379.0
     

     






      

          
GC ORFID: 43200How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2153Glimmer Score: 

          
Start: 1681930Stop: 1684128

          
Length: 2199


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2153 translation of PA2153Homolog Bit Score 1550.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1411 (ORF: BLASTX 1030.0), t_PA2153 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGAACCGATGTCCGAACGCGAACGCTGGCAACTGGAGCTGGACAAGCTGCAGCGCGGCCTGCAGGGC 
GATCCATTCGCCTTCCTCGGCCCGCAGCGCGACCCCGGCGGCGAAGGCGGCGTGCTGCGCGCCTACCTGCCC 
GGCGCGCAGCGCGTCGAGCTGCTCGACGAAGACGGCGCGACGCTCGCCGAACTGGAGCAGAGCGACCCCGGC 
AGCGGCTTGTTCCAGCGCCGCCTGGAGCGCCTGCCGCCGCGCTACCGCCTGCGCGTGCACTGGCCGGACGGC 
GTGCAGGAAAGCGAAGACCCCTATGCCTTCGGGCCGTTGCTCGGCGAACTCGACCTTTACCTGTTCGCCGAA 
GGCAACCACCGCCAACTGGCCTCCTGCCTGGGCGCGCAACTGACCCGTCACGAAGGCGTGGAGGGCGTGCGC 
TTCGCGGTCTGGGCGCCGAACGCCGTGCGCGTCTCGGTGGTCGGCGATTTCAACGGCTGGGACGGGCGCCGT 
CACCCGATGCGACGACGCTATCCCTCCGGCGTCTGGGAACTGTTCGTCCCGCGCCTCGGCGAGGGCGAACTG 
TACAAGTACGAGTTGCAGGGCCACGACGGCCTCCTGCCGCTGAAGGCCGACCCCCTGGCGCTGGCCTGCGAG 
ACGCCGCCCGGAACCGCTTCGAAGACCTGCGCGGCGTTGCGCCACGAGTGGCGCGACCAGGACTGGCTGGCC 
CGGCGCGCGGAGCGCCAGGGCTATGCGGCACCGCTGTCGATCTACGAAGTCCACGCCGGCTCCTGGCGCCAC 
CGCGACGGCCGGCCGCCGCACTGGTCGGAACTGGCGGAGGAGCTGATTCCCTACGTGCGCCAGCTCGGCTTC 
ACCCATATCGAGCTGATGCCGGTGATGGAACATCCCTTCGGCGGCTCCTGGGGCTACCAGCCGCTCGGGTTG 
TTCGCCCCCACCGCGCGCTACGGCACGCCGGAGGACTTCGCCGCGTTCGTCGACGCCTGCCACCAGGCCGGC 
ATCGGCGTGATCCTCGACTGGGTGCCGGCGCATTTTCCCACCGACGCCCACGGCCTGGGCCGTTTCGACGGC 
ACGGCGCTGTACGAGTACGAACATCCCTTCGAAGGCTTCCACCAGGACTGGGATACCTACATCTACAACCTT 
GGCCGCAGCGAGGTGCACGGCTTCATGCTGGCCTCGGCGCTGCACTGGCTGCGCACCTACCACGTCGACGGC 
CTGCGGGTGGATGCGGTGGCCTCGATGCTCTACCGCGACTATTCGCGCAAGGAAGGCGAATGGCTGCCGAAC 
CGCCATGGCGGCCGGGAGAACCTGGAGGCCATCGACTTCCTCCATCACCTCAACCAGGTGGTCGCCAGCGAG 
ACCCCCGGCGCGCTGGTGATCGCCGAGGAATCCACCGCCTGGCCCGGCGTCAGCCGTCCGGTGGCGGAGGGC 
GGCCTGGGCTTCAGCCATAAGTGGAACATGGGCTGGATGCACGACAGCCTGGCGTACATCGGCGAAGACCCG 
CTGCACCGGCGCTATCACCACCACCAGTTGACCTTCGGCCTGCTCTACGCCTTCTCCGAGCATTTCATCCTG 
CCGATCTCCCACGACGAGGTGGTACATGGCAAGCACTCGCTACTGGACAAGATGCCCGGCGACCGCTGGCAG 
AAGTTCGCCAACCTGCGCCTGTACCTGAGCTTCATGTGGTCGCACCCGGGCAAGAAGCTGCTGTTCATGGGC 
TGCGAGTTCGGCCAGTGGCGCGAGTGGAACCACGACGGCGAACTGGACTGGTACCTGCTGCGCTATCCGGAG 
CATCAGGGCGTACAGGATCTGGTGGCGGCCCTCAACCGGCTGTACCGCGATCTCCCCGCACTGCATGCGCGC 
GACGGCGAGGCGCTCGGCTTCGAATGGCTGATCGGCGACGACCAGGCGAACAGTGTCTACGCCTGGCTGCGC 
CACGCGGCCGGCGAGCCATCGCTGCTGGCGGTACACAATTTCACCCCGGTGCCGCGCCAGGGCTATCGGATC 
GGCGTACCGCAAGGCGGCGACTGGGACGTGCTGCTCAATAGCGACGCCCAGGCGTTCGCCGGTTCCGGCTCA 
GGCAGCCAGGGCCGCGTCTCCAGCGAGTCGTGCGGCGCCCATGGCCAGGCGCAGTCGCTGCTGCTCGACCTG 
CCGCCGCTGGGCACGCTGCTGCTGCGTCCGGCCGGCTGA