ViewGene
Locus Name:
GeneID:
Location:

GeneID:1966
TrivialName: PA14_10270
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_10270
PAO1 Ortholog Locus:PA4149
Sequence Length:1041
Protein Length:346
Start: 885348
Stop: 884308
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07536.1|AE004831_8 (AE004831) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 345/346 (99%), Positives = 345/346 (99%)
Sequence:
ATGACCCCGCCCTCGCCGATCGTCGGCATCATCGCCAACCCGGCCTCCGGTCGCGACCTGCGTCGCCTCACC
GCCCATGCCGGGCTGCACTCCAGCACCGACAAGGCATCGGTGGTGCAACGCCTGCTCGCCGCCTTCGGCGCC
ACCGGCATCCGCCATGCGTTGCTGCCGCCGGACATGACCGGCATCGCCGCCGCCGTGCTCCGGGCCAGCCAG
ACCCGCCAGGCCCGCGACGGCCACTGGCCGTTCCTGGAGTTCCTCGACATGCCGCTGCGCCAGAGCGTCGAG
GATACCCGCCTGGCGGCGCGGCGCATGGTCGAGCGCGGCGTGGCGCTGATCGCCGTGCTCGGCGGCGACGGC
ACGCACAAGGCAGTGGCCGCCGAGGTCGGCGACGTCCCCCTGCTGGCGCTGTCCACCGGGACCAACAACGCC
TTCCCGGAGCTGCGCGAAGCCACCGGCGCGGGGCTCGCCGGCGGCCTCTGCGCCAGCAACCGGGTGCCGCCG
GAGATCGGCCTGCGACGCAACAAGCGCCTGCTGGTGCGCGAGCCGCGCCGCGGGCTCTGCGAGTGGGCGCTG
GTGGACGTCGCGGTGTCGCCGCAGCCGTTCATCGGCGCCCGCGCCATCAGTCGCGCCGAAGACCTCGCCGAA
GTCTTCGTCAGCTTCGCCGAACCCCACGCCATCGGCCTTTCGGCGCTTTGCGGACTGTGGTCGCCGGTGAGC
CGCGAGGAGCCCCACGGCGCGTGGATGCGCCTGCATCCGGACGCCCGCGAGAGCCTGCTGGTGCCGCTGGCG
CCGGGCCTGCTGGTGGGCTGCGGGGTCTCTGCCGCCGGCTACCTGCAACCCGGCGTGGCCCACGCGCCGAGC
CTGTCCAGCGGGACCCTGGCGCTGGACGGAGAGCGCGAGATCGAGTTCAGCGCCACCGACCGCCCGAGCATC
ACCCTCGACCCTTCCGACCCGTTCAGCGTCGATGTGCCGGCCACCCTCGCCTACGCCGCCCGCCATCGCCTG
CTGGCCGGCCAGCGAACCCCGATGACCCCGTGA
Translation:
MTPPSPIVGIIANPASGRDLRRLTAHAGLHSSTDKASVVQRLLAAFGATGIRHALLPPDMTGIAAAVLRASQ
TRQARDGHWPFLEFLDMPLRQSVEDTRLAARRMVERGVALIAVLGGDGTHKAVAAEVGDVPLLALSTGTNNA
FPELREATGAGLAGGLCASNRVPPEIGLRRNKRLLVREPRRGLCEWALVDVAVSPQPFIGARAISRAEDLAE
VFVSFAEPHAIGLSALCGLWSPVSREEPHGAWMRLHPDARESLLVPLAPGLLVGCGVSAAGYLQPGVAHAPS
LSSGTLALDGEREIEFSATDRPSITLDPSDPFSVDVPATLAYAARHRLLAGQRTPMTP*
AnnotationID:1975GeneID:1966
AnnotatorUID: diggins
Modification Date/Time: 2005-06-07 08:08:39
Gene Name:
Confidence Code:3
GeneProduct:putative acetoin catabolism protein
Cell Localization:()
Synonyms:acoX
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam01513
Secondary Functional Category(ies):putative enzyme
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66183.1|AE016245_12 (AE016776) acetoin catabolism protein [Pseudomonas putida KT2440]
          Length = 351

 Score =  503 bits (1294), Expect = e-142
 Identities = 259/341 (75%), Positives = 284/341 (83%)

>gb|AAA21947.1| (M66060) protein X [Ralstonia eutropha]
          Length = 359

 Score =  268 bits (685), Expect = 4e-71
 Identities = 156/346 (45%), Positives = 209/346 (60%), Gaps = 9/346 (2%)






 
Structural Features:
pfam01513, NAD_kinase, ATP-NAD kinase. 
CD-Length = 280 residues,  99.6% aligned
Score =  128 bits (324), Expect = 7e-31
Identities = 89/321 (27%), Positives = 123/321 (38%), Gaps = 45/321 (14%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3935




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21081




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36884




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1966_cdd.html


    		




 
URL:

















    ReferenceID:36980




  
Author/Investigator(s):
        Priefert H, Hein S, Kruger N, Zeh K, Schmidt B, Steinbuchel A.





  
Title:
        Identification and molecular characterization of the Alcaligenes eutrophus H16 aco operon genes involved in acetoin catabolism.




  
PubMed:
        2061286


MedLine:
        




    
Source:
J Bacteriol. 1991 Jul;173(13):4056-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2061286

















    ReferenceID:36987




  
Author/Investigator(s):
        Kruger N, Oppermann FB, Lorenzl H, Steinbuchel A.





  
Title:
        Biochemical and molecular characterization of the Clostridium magnum acetoin dehydrogenase enzyme system.




  
PubMed:
        8206840


MedLine:
        




    
Source:
J Bacteriol. 1994 Jun;176(12):3614-30.

Erratum in:
    * J Bacteriol 1994 Aug;176(16):5193.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8206840

















    ReferenceID:36996




  
Author/Investigator(s):
        Huang M, Oppermann FB, Steinbuchel A.





  
Title:
        Molecular characterization of the Pseudomonas putida 2,3-butanediol catabolic pathway.




  
PubMed:
        7813883


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1994 Dec 1;124(2):141-50.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=95113288&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:1966
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3429
                           PA4149_tr
                           translation of PA4149
                           346
                           99.71
                           99.71
                           0
                           1762.0
     

     
     
105503
                           gb|AAG07536.1|AE004831_8
                           (AE004831) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           346
                           99.71
                           99.71
                           0
                           1762.0
     

     
     
105504
                           gb|AAN66183.1|AE016245_12
                           (AE016776) acetoin catabolism protein [Pseudomonas putida KT2440]
                           351
                           74.64
                           81.76
                           5
                           1295.0
     

     
     
105505
                           gb|AAB58978.1|
                           (L35343) unknown [Pseudomonas putida]
                           351
                           72.36
                           79.77
                           5
                           1235.0
     

     
     
105506
                           gb|AAA21947.1|
                           (M66060) protein X [Ralstonia eutropha]
                           361
                           43.76
                           58.17
                           17
                           689.0
     

     






      

          
GC ORFID: 44968How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4149Glimmer Score: 

          
Start: 4068589Stop: 4069629

          
Length: 1041


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4149 translation of PA4149Homolog Bit Score 683.0

          
Other Homologs:  t_PA4149 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCCCGCCCTCGCCGATCGTCGGCATCATCGCCAACCCGGCCTCCGGTCGCGACCTGCGTCGCCTCACC 
GCCCATGCCGGGCTGCACTCCAGCACCGACAAGGCATCGGTGGTGCAACGCCTGCTCGCCGCCTTCGGCGCC 
ACCGGCATCCGCCATGCGTTGCTGCCGCCGGACATGACCGGCATCGCCGCCGCCGTGCTCCGGGCCAGCCAG 
ACCCGCCAGGCCCGCGACGGCCACTGGCCGTTCCTGGAGTTCCTCGACATGCCGCTGCGCCAGAGCGTCGAG 
GATACCCGCCTGGCGGCGCGGCGCATGGTCGAGCGCGGCGTGGCGCTGATCGCCGTGCTCGGCGGCGACGGC 
ACGCACAAGGCAGTGGCCGCCGAGGTCGGCGACGTCCCCCTGCTGGCGCTGTCCACCGGGACCAACAACGCC 
TTCCCGGAGCTGCGCGAAGCCACCGGCGCGGGGCTCGCCGGCGGCCTCTGCGCCAGCAACCGGGTGCCGCCG 
GAGATCGGCCTGCGACGCAACAAGCGCCTGCTGGTGCGCGAGCCGCGCCGCGGGCTCTGCGAGTGGGCGCTG 
GTGGACGTCGCGGTGTCGCCGCAGCCGTTCATCGGCGCCCGCGCCATCAGTCGCGCCGAAGACCTCGCCGAA 
GTCTTCGTCAGCTTCGCCGAACCCCACGCCATCGGCCTTTCGGCGCTTTGCGGACTGTGGTCGCCGGTGAGC 
CGCGAGGAGCCCCACGGCGCGTGGATGCGCCTGCATCCGGACGCCCGCGAGAGCCTGCTGGTGCCGCTGGCG 
CCGGGCCTGCTGGTGGGCTGCGGGGTCTCTGCCGCCGGCTACCTGCAACCCGGCGTGGCCCACGCGCCGAGC 
CTGTCCAGCGGGACCCTGGCGCTGGACGGAGAGCGCGAGATCGAGTTCAGCGCCACCGACCGCCCGAGCATC 
ACCCTCGACCCTTCCGACCCGTTCAGCGTCGATGTGCCGGCCACCCTCGCCTACGCCGCCCGCCATCGCCTG 
CTGGCCGGCCAGCGAACCCCGATGACCCCGTGA