ViewGene
Locus Name:
GeneID:
Location:

GeneID:1956
TrivialName: PA14_00300
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_00300
PAO1 Ortholog Locus:PA0026
Sequence Length:987
Protein Length:328
Start: 27667
Stop: 28653
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03416.1|AE004442_3 (AE004442) hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 327/328 (99%), Positives = 327/328 (99%)
Sequence:
ATGAAAACCTTCGCCCGCCTGGCCGCCGGTCTCTGCATCTGCGCCATCGCCAGCCAGGCCAACGCCTGGTCG
CAACCGACCCACAAGAACATCGTCAAGGACGCCCTGGCCTTCATGAACTCCTCCTACGCCACCGAGGAGATG
CGTCAGGCCTACCGGTTCTACGTGAGCGCCGCCGGCAGCGAGGCGCAGGCCGGGGAGATCCTCGGCCAGGCG
GCCTTCGACGTGGACGACTTCAAGGACACCCGTCTCGGGGGCTGGTGGGTCGGCTACGAACACGCGCCGCTG
TGGGGCGCGGCTTCGGGCATCGTCAACTACACCTCGTACTGGCACTTCCTCAACCTGGCCCGCGACGGCGAT
TCCCATGGCAACCCCCACGGCGGCTACGACTACCGCTACCACAAGGTCGACGGCGGCATCGCCGACGTCGAC
TGGTACGCCATGGTCTATCTCTACAACCGCGAGCTGAAGCGCGAGGACTTCGACACCACCGAGGCGCACTAC
CGCCAGGGCACCCGCTCGGACTGGCAGGAGCACTACGGCGACTTCCAGACCGCCGCCTTCCAGCCGATCGAC
AACCTCGCCACCTACTGGTTCGAGCAGTTCCGCGCCGCGCCTTCGCTGCAAACCATCGGCTATGCCCTGCAC
GCCACCGGCGACGTGGCCCAGCCGCACCATGTGTGGATCACCTCGGCCAACGGCCATTCCAGCTGGGAAGGC
TGGGTCGACGACCACTACGCCAGCGAGAAGCTCAACGACCCGGCGGCGGTGGCCAACCTGGTGGGGCGCTAC
GATCCGAGCAAGAGCATCCGCGACCTGCTCACCCAGACCGGCCAGGTCGCCTACGCGCGTCCCGAGCCGCTC
TACGACACCAGCTACGAGACGCGCCTGCGGGTGGCGAAGGAGCTGATTCCCGAGTCCATCGCCCTGACCGTC
ACCGTACTGACCAAGGGCGCCAACAGCTTCGATGCACAGACTGCTCTCTGA
Translation:
MKTFARLAAGLCICAIASQANAWSQPTHKNIVKDALAFMNSSYATEEMRQAYRFYVSAAGSEAQAGEILGQA
AFDVDDFKDTRLGGWWVGYEHAPLWGAASGIVNYTSYWHFLNLARDGDSHGNPHGGYDYRYHKVDGGIADVD
WYAMVYLYNRELKREDFDTTEAHYRQGTRSDWQEHYGDFQTAAFQPIDNLATYWFEQFRAAPSLQTIGYALH
ATGDVAQPHHVWITSANGHSSWEGWVDDHYASEKLNDPAAVANLVGRYDPSKSIRDLLTQTGQVAYARPEPL
YDTSYETRLRVAKELIPESIALTVTVLTKGANSFDAQTAL*
AnnotationID:1965GeneID:1956
AnnotatorUID: danlee
Modification Date/Time: 2005-07-06 11:11:41
Gene Name:plcB
Confidence Code:1
GeneProduct:phospholipase C, PlcB
Cell Localization:(9) Secreted
Synonyms:PA0026
Cell Localization Confidence Code:1
MolecularFunction:
Functional Category:(14) Hypothetical, unclassified, unknown
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:






 
Structural Features:
CDD Search: No hits above 0.01 threshold.






   
Genomic Context:
PA0026, PA0027 & PA0028 constitute an operon






   
Comment:
PlcB is required for directed twitching motility up a gradient of phosphatidylcholine (PC), 
or phosphatidylethanolamine (PE) with specific type of fatty acid residues.
PlcB can hyrolyse both PC and PE, and secreted via the Sec pathway. Proteins encoded


















    ReferenceID:3915




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21061




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:38188




  
Author/Investigator(s):
        Barker AP, Vasil AI, Filloux A, Ball G, Wilderman PJ, Vasil ML.





  
Title:
        A novel extracellular phospholipase C of Pseudomonas aeruginosa is required for phospholipid chemotaxis.




  
PubMed:
        15306013


MedLine:
        




    
Source:
Mol Microbiol. 2004 Aug;53(4):1089-98.






    
Reference Type: 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1956
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3418
                           PA0026_tr
                           translation of PA0026
                           328
                           99.69
                           99.69
                           0
                           1767.0
     

     
     
105396
                           gb|AAG03416.1|AE004442_3
                           (AE004442) hypothetical protein PA0026 [Pseudomonas aeruginosa PAO1]
                           328
                           99.69
                           99.69
                           0
                           1767.0
     

     






      

          
GC ORFID: 44958How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0026Glimmer Score: 

          
Start: 5829004Stop: 5829990

          
Length: 987


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0026 translation of PA0026Homolog Bit Score 685.0

          
Other Homologs:  t_PA0026 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAAACCTTCGCCCGCCTGGCCGCCGGTCTCTGCATCTGCGCCATCGCCAGCCAGGCCAACGCCTGGTCG 
CAACCGACCCACAAGAACATCGTCAAGGACGCCCTGGCCTTCATGAACTCCTCCTACGCCACCGAGGAGATG 
CGTCAGGCCTACCGGTTCTACGTGAGCGCCGCCGGCAGCGAGGCGCAGGCCGGGGAGATCCTCGGCCAGGCG 
GCCTTCGACGTGGACGACTTCAAGGACACCCGTCTCGGGGGCTGGTGGGTCGGCTACGAACACGCGCCGCTG 
TGGGGCGCGGCTTCGGGCATCGTCAACTACACCTCGTACTGGCACTTCCTCAACCTGGCCCGCGACGGCGAT 
TCCCATGGCAACCCCCACGGCGGCTACGACTACCGCTACCACAAGGTCGACGGCGGCATCGCCGACGTCGAC 
TGGTACGCCATGGTCTATCTCTACAACCGCGAGCTGAAGCGCGAGGACTTCGACACCACCGAGGCGCACTAC 
CGCCAGGGCACCCGCTCGGACTGGCAGGAGCACTACGGCGACTTCCAGACCGCCGCCTTCCAGCCGATCGAC 
AACCTCGCCACCTACTGGTTCGAGCAGTTCCGCGCCGCGCCTTCGCTGCAAACCATCGGCTATGCCCTGCAC 
GCCACCGGCGACGTGGCCCAGCCGCACCATGTGTGGATCACCTCGGCCAACGGCCATTCCAGCTGGGAAGGC 
TGGGTCGACGACCACTACGCCAGCGAGAAGCTCAACGACCCGGCGGCGGTGGCCAACCTGGTGGGGCGCTAC 
GATCCGAGCAAGAGCATCCGCGACCTGCTCACCCAGACCGGCCAGGTCGCCTACGCGCGTCCCGAGCCGCTC 
TACGACACCAGCTACGAGACGCGCCTGCGGGTGGCGAAGGAGCTGATTCCCGAGTCCATCGCCCTGACCGTC 
ACCGTACTGACCAAGGGCGCCAACAGCTTCGATGCACAGACTGCTCTCTGA