ViewGene
Locus Name:
GeneID:
Location:

GeneID:1955
TrivialName: PA14_28690
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_28690
PAO1 Ortholog Locus:PA2740
Sequence Length:1017
Protein Length:338
Start: 2470426
Stop: 2471442
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06128.1|AE004702_5 (AE004702) phenylalanyl-tRNA synthetase, alpha-subunit [Pseudomonas aeruginosa PAO1]
Identities = 337/338 (99%), Positives = 337/338 (99%)
Sequence:
ATGGAAAACCTGGATGCGCTGGTCTCTCAAGCATTGGAGGCTGTGCGACACACCGAAGACGTCAATGCCCTG
GAGCAGATCCGGGTTCACTACCTCGGCAAGAAAGGCGAACTGACCCAGGTGATGAAGACCCTGGGCGACCTG
CCGGCCGAAGAGCGGCCGAAGGTCGGCGCGCTGATCAACGTGGCCAAGGAAAAGGTCCAGGATGCGCTGAAC
GCGCGTAAGACCGAGCTGGAAGGGGCCGCGCTGGCCGCCCGGCTGGCCGCGGAGCGCATCGATGTCACCCTG
CCCGGTCGTGGCCAGCTGAGCGGCGGCCTGCATCCGGTCACCCGCACGCTGGAGCGCATCGAGCAGTGCTTC
AGCCGCATCGGCTACGAAGTCGCCGAAGGCCCGGAAGTCGAAGACGACTACCACAACTTCGAAGCGCTCAAT
ATTCCGGGCCACCACCCGGCGCGTGCCATGCACGATACTTTCTATTTCAATGCGAACATGCTGCTGCGTACC
CACACCTCACCGGTGCAGGTGCGGACCATGGAGTCCCAGCAGCCGCCGATCCGCATTGTCTGCCCGGGGCGC
GTCTACCGCTGCGACTCCGACCTGACCCACTCGCCGATGTTCCACCAGGTGGAAGGCCTGTTGGTGGACGAG
GGCGTCAGCTTCGCCGACCTCAAGGGCACCATCGAGGAATTCCTCCGCGCCTTCTTCGAGAAGCAGCTCGAA
GTGCGCTTCCGTCCGTCCTTCTTCCCCTTCACCGAGCCGTCGGCCGAAGTGGACATCCAGTGCGTGATCTGC
AGCGGCAACGGCTGCCGCGTGTGCAAGCAGACCGGCTGGCTGGAAGTCATGGGTTGCGGCATGGTGCACCCG
AACGTGCTGCGCATGTCCAACATCGATCCCGAGAAATTCCAGGGCTTCGCCTTCGGCATGGGCGCCGAGCGC
CTGGCCATGCTGCGCTACGGGGTGAACGACCTGCGTCTGTTCTTCGATAACGATCTGCGCTTCCTCGGACAA
TTCCGCTAG
Translation:
MENLDALVSQALEAVRHTEDVNALEQIRVHYLGKKGELTQVMKTLGDLPAEERPKVGALINVAKEKVQDALN
ARKTELEGAALAARLAAERIDVTLPGRGQLSGGLHPVTRTLERIEQCFSRIGYEVAEGPEVEDDYHNFEALN
IPGHHPARAMHDTFYFNANMLLRTHTSPVQVRTMESQQPPIRIVCPGRVYRCDSDLTHSPMFHQVEGLLVDE
GVSFADLKGTIEEFLRAFFEKQLEVRFRPSFFPFTEPSAEVDIQCVICSGNGCRVCKQTGWLEVMGCGMVHP
NVLRMSNIDPEKFQGFAFGMGAERLAMLRYGVNDLRLFFDNDLRFLGQFR*
AnnotationID:1964GeneID:1955
AnnotatorUID: diggins
Modification Date/Time: 2005-04-05 07:07:40
Gene Name:pheS
Confidence Code:2
GeneProduct:phenylalanyl-tRNA synthetase, alpha-subunit
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0016
Secondary Functional Category(ies):
EC Number:6.1.1.20
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAL87034.1|AF332624_5 (AF332624) phenylalanyl-tRNA-synthetase alpha subunit [Azotobacter
           vinelandii]
          Length = 338

 Score =  619 bits (1595), Expect = e-176
 Identities = 297/338 (87%), Positives = 315/338 (93%)

>gb|AAN68081.1|AE016440_1 (AE016783) phenylalanyl-tRNA synthetase, alpha subunit [Pseudomonas
           putida KT2440]
          Length = 338

 Score =  613 bits (1580), Expect = e-175
 Identities = 298/338 (88%), Positives = 314/338 (92%)

>gb|AAO55893.1| (AE016864) phenylalanyl-tRNA synthetase, alpha subunit [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 338

 Score =  608 bits (1567), Expect = e-173
 Identities = 293/338 (86%), Positives = 313/338 (92%)






 
Structural Features:
COG0016, PheS, Phenylalanyl-tRNA synthetase alpha subunit 
[Translation, ribosomal structure and biogenesis]
CD-Length = 335 residues,  99.4% aligned
Score =  468 bits (1207), Expect = 3e-133
Identities = 192/343 (55%), Positives = 242/343 (70%), Gaps = 17/343 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3913




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21059




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36290




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1955_cdd.html


    		




 
URL:

















    ReferenceID:36369




  
Author/Investigator(s):
        Liao X, Charlebois I, Ouellet C, Morency MJ, Dewar K, Lightfoot J, Foster J, Siehnel R, Schweizer H, Lam JS, Hancock RE, Levesque RC.





  
Title:
        Physical mapping of 32 genetic markers on the Pseudomonas aeruginosa PAO1 chromosome.




  
PubMed:
        8581173


MedLine:
        




    
Source:
Microbiology. 1996 Jan;142 ( Pt 1):79-86.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8581173

















    ReferenceID:36374




  
Author/Investigator(s):
        Savopoulos JW, Hibbs M, Jones EJ, Mensah L, Richardson C, Fosberry A, Downes R, Fox SG, Brown JR, Jenkins O.





  
Title:
        Identification, cloning, and expression of a functional phenylalanyl-tRNA synthetase (pheRS) from Staphylococcus aureus.




  
PubMed:
        11281723


MedLine:
        




    
Source:
Protein Expr Purif. 2001 Apr;21(3):470-84.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11281723

















    ReferenceID:36375




  
Author/Investigator(s):
        Keller B, Kast P, Hennecke H.





  
Title:
        Cloning and sequence analysis of the phenylalanyl-tRNA synthetase genes (pheST) from Thermus thermophilus.




  
PubMed:
        1451792


MedLine:
        




    
Source:
FEBS Lett. 1992 Apr 13;301(1):83-8.

Erratum in:
    * FEBS Lett. 1992 Sep 28;310(2):204.








    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1451792

















    ReferenceID:36376




  
Author/Investigator(s):
        Brakhage AA, Wozny M, Putzer H.





  
Title:
        Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase genes.




  
PubMed:
        2127701


MedLine:
        




    
Source:
Biochimie. 1990 Oct;72(10):725-34.

Erratum in:
    * Biochimie. 1991 Jan;73(1):127.








    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2127701

















    ReferenceID:36377




  
Author/Investigator(s):
        Fayat G, Mayaux JF, Sacerdot C, Fromant M, Springer M, Grunberg-Manago M, Blanquet S.





  
Title:
        Escherichia coli phenylalanyl-tRNA synthetase operon region. Evidence for an attenuation mechanism. Identification of the gene for the ribosomal protein L20.




  
PubMed:
        6317865


MedLine:
        




    
Source:
J Mol Biol. 1983 Dec 15;171(3):239-61.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6317865









           
Homologs By Global Alignment 

           

       



     
Gene ID:1955
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3417
                           PA2740_tr
                           translation of PA2740
                           338
                           99.70
                           99.70
                           0
                           1768.0
     

     
     
105257
                           gb|AAG06128.1|AE004702_5
                           (AE004702) phenylalanyl-tRNA synthetase, alpha-subunit [Pseudomonas aeruginosa PAO1]
                           338
                           99.70
                           99.70
                           0
                           1768.0
     

     
     
105259
                           gb|AAN68081.1|AE016440_1
                           (AE016783) phenylalanyl-tRNA synthetase, alpha subunit [Pseudomonas putida KT2440]
                           338
                           88.16
                           92.89
                           0
                           1580.0
     

     
     
105258
                           gb|AAL87034.1|AF332624_5
                           (AF332624) phenylalanyl-tRNA-synthetase alpha subunit [Azotobacter vinelandii]
                           338
                           87.86
                           93.19
                           0
                           1595.0
     

     
     
105260
                           gb|AAO55893.1|
                           (AE016864) phenylalanyl-tRNA synthetase, alpha subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           338
                           86.68
                           92.60
                           0
                           1567.0
     

     






      

          
GC ORFID: 44957How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2740Glimmer Score: 

          
Start: 2483500Stop: 2482484

          
Length: 1017


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA2740 translation of PA2740Homolog Bit Score 685.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1167 (ORF: BLASTX 610.0), t_PA2740 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGAAAACCTGGATGCGCTGGTCTCTCAAGCATTGGAGGCTGTGCGACACACCGAAGACGTCAATGCCCTG 
GAGCAGATCCGGGTTCACTACCTCGGCAAGAAAGGCGAACTGACCCAGGTGATGAAGACCCTGGGCGACCTG 
CCGGCCGAAGAGCGGCCGAAGGTCGGCGCGCTGATCAACGTGGCCAAGGAAAAGGTCCAGGATGCGCTGAAC 
GCGCGTAAGACCGAGCTGGAAGGGGCCGCGCTGGCCGCCCGGCTGGCCGCGGAGCGCATCGATGTCACCCTG 
CCCGGTCGTGGCCAGCTGAGCGGCGGCCTGCATCCGGTCACCCGCACGCTGGAGCGCATCGAGCAGTGCTTC 
AGCCGCATCGGCTACGAAGTCGCCGAAGGCCCGGAAGTCGAAGACGACTACCACAACTTCGAAGCGCTCAAT 
ATTCCGGGCCACCACCCGGCGCGTGCCATGCACGATACTTTCTATTTCAATGCGAACATGCTGCTGCGTACC 
CACACCTCACCGGTGCAGGTGCGGACCATGGAGTCCCAGCAGCCGCCGATCCGCATTGTCTGCCCGGGGCGC 
GTCTACCGCTGCGACTCCGACCTGACCCACTCGCCGATGTTCCACCAGGTGGAAGGCCTGTTGGTGGACGAG 
GGCGTCAGCTTCGCCGACCTCAAGGGCACCATCGAGGAATTCCTCCGCGCCTTCTTCGAGAAGCAGCTCGAA 
GTGCGCTTCCGTCCGTCCTTCTTCCCCTTCACCGAGCCGTCGGCCGAAGTGGACATCCAGTGCGTGATCTGC 
AGCGGCAACGGCTGCCGCGTGTGCAAGCAGACCGGCTGGCTGGAAGTCATGGGTTGCGGCATGGTGCACCCG 
AACGTGCTGCGCATGTCCAACATCGATCCCGAGAAATTCCAGGGCTTCGCCTTCGGCATGGGCGCCGAGCGC 
CTGGCCATGCTGCGCTACGGGGTGAACGACCTGCGTCTGTTCTTCGATAACGATCTGCGCTTCCTCGGACAA 
TTCCGCTAG