ViewGene
Locus Name:
GeneID:
Location:

GeneID:1941
TrivialName: PA14_17180
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_17180
PAO1 Ortholog Locus:PA3646
Sequence Length:1062
Protein Length:353
Start: 1473505
Stop: 1474566
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07034.1|AE004784_7 (AE004784) UDP-3-O-[3-hydroxylauroyl] glucosamine N-acyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 351/353 (99%), Positives = 353/353 (100%)
Sequence:
ATGATGAGTACCTTGTCCTACACCCTGGGCCAGCTGGCCGCGCATGTCGGCGCGGAACTCCGTGGCGACGCG
GACCTGCCGATCCAGGGCCTGGCGACCTTGCAGGAGGCCGGACCGGCTCAGCTGAGCTTCCTGGCCAACCCG
CAGTATCGCAAGTACCTGCCCGAAAGCCGTGCCGGCGCCGTCCTGCTGACGGCTGCCGACGCCGACGGGTTC
TCCGGTACCGCGCTGGTGGTGGCCAATCCCTACCTGGCCTATGCCAGCCTGTCGCACCTGTTCGATCGCAAA
CCGAAGGCGGCCGCCGGTATCCATCCCACCGCCATCGTCGCGGCGGACGCCGAGGTCGATCCGAGCGCCAGC
GTCGGCGCCTATGCGGTGATCGAGAGTGGCGCCCGCATCGGCGCTGGCGTCAGCATCGGCGCGCACTGCGTA
ATCGGCGCCCGTAGCGTGATCGGCGAAGGCGGCTGGCTGGCGCCGCGGGTCACGCTCTACCACGACGTCACC
ATCGGCGCGCGGGTGAGTATCCAGTCGGGCGCGGTGATCGGCGGCGAAGGCTTCGGTTTCGCCAACGAGAAG
GGCGTCTGGCAGAAGATCGCCCAGATCGGCGGGGTGACCATCGGCGACGACGTCGAGATCGGCGCGAACACC
ACCATCGACCGTGGAGCACTGTCCGATACGCTGATCGGCAACGGTGTGAAGCTGGACAACCAGATCATGATC
GCGCACAACGTGCAGATCGGCGATCATACCGCCATGGCCGCTTGCGTCGGCATTTCCGGCAGCGCCAAGATC
GGCAGGCATTGCATGCTCGCCGGCGGCGTTGGCCTGGTCGGCCATATCGAGATCTGCGACAACGTTTTCGTG
ACCGGCATGACCATGGTGACCCGCTCGATCACCGAGCCGGGATCGTATTCCTCCGGTACCGCCATGCAACCG
GCGGCCGAATGGAAAAAAAGCGCCGCGCGCATTCGTCAACTGGACGACATGGCGCGTCGACTTCAGCAGCTG
GAAAAACGCCTGGCTGCCGTGACCTCAAGCGGTGACGCTTCATCTGATGCGTAA
Translation:
MMSTLSYTLGQLAAHVGAELRGDADLPIQGLATLQEAGPAQLSFLANPQYRKYLPESRAGAVLLTAADADGF
SGTALVVANPYLAYASLSHLFDRKPKAAAGIHPTAIVAADAEVDPSASVGAYAVIESGARIGAGVSIGAHCV
IGARSVIGEGGWLAPRVTLYHDVTIGARVSIQSGAVIGGEGFGFANEKGVWQKIAQIGGVTIGDDVEIGANT
TIDRGALSDTLIGNGVKLDNQIMIAHNVQIGDHTAMAACVGISGSAKIGRHCMLAGGVGLVGHIEICDNVFV
TGMTMVTRSITEPGSYSSGTAMQPAAEWKKSAARIRQLDDMARRLQQLEKRLAAVTSSGDASSDA*
AnnotationID:1950GeneID:1941
AnnotatorUID: diggins
Modification Date/Time: 2005-06-19 19:07:48
Gene Name:lpxD
Confidence Code:2
GeneProduct:UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1044,pfam04613
Secondary Functional Category(ies):fatty acid and phospholipid metabolism
EC Number:2.3.1.-
Status:ACTIVE
Pathway:
Lipid A biosynthesis; third step.
Homology:
>gb|AAN67222.1|AE016349_3 (AE016779) UDP-3-O-[3-hydroxymyristoyl]  glucosamine
           N-acyltransferase [Pseudomonas putida KT2440]
          Length = 351

 Score =  561 bits (1445), Expect = e-159
 Identities = 280/349 (80%), Positives = 310/349 (88%)

>gb|AAB08608.1| (U70214) UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase
           [Escherichia coli]
          Length = 341

 Score =  340 bits (873), Expect = 6e-93
 Identities = 173/335 (51%), Positives = 229/335 (68%)
Structural Features:
COG1044, LpxD, UDP-3-O-[3-hydroxymyristoyl]
CD-Length = 338 residues,  99.7% aligned
Score =  377 bits (970), Expect = 2e-105
Identities = 183/338 (54%), Positives = 229/338 (67%), Gaps = 3/338 (0%)

pfam04613, LpxD, UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase
CD-Length = 102 residues, 100.0% aligned
Score =  115 bits (289), Expect = 2e-26
Identities = 52/102 (50%), Positives = 69/102 (67%), Gaps = 3/102 (2%)
Genomic Context:






   
Comment:


















    ReferenceID:3885




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21032




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31897




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1941_cdd.html


    		




 
URL:

















    ReferenceID:34215




  
Author/Investigator(s):
        Steeghs L, Jennings MP, Poolman JT, van der Ley P.





  
Title:
        Isolation and characterization of the Neisseria meningitidis lpxD-fabZ-lpxA gene cluster involved in lipid A biosynthesis.




  
PubMed:
        9197543


MedLine:
        




    
Source:
Gene. 1997 May 6;190(2):263-70.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9197543









           
Homologs By Global Alignment 

           

       



     
Gene ID:1941
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3397
                           PA3646_tr
                           translation of PA3646
                           353
                           99.43
                           99.99
                           0
                           1775.0
     

     
     
104936
                           gb|AAG07034.1|AE004784_7
                           (AE004784) UDP-3-O-[3-hydroxylauroyl] glucosamine N-acyltransferase [Pseudomonas aeruginosa PAO1]
                           353
                           99.43
                           99.99
                           0
                           1775.0
     

     
     
104937
                           gb|AAN67222.1|AE016349_3
                           (AE016779) UDP-3-O-[3-hydroxymyristoyl]  glucosamine N-acyltransferase [Pseudomonas putida KT2440]
                           353
                           79.32
                           87.81
                           2
                           1444.0
     

     
     
104938
                           gb|AAO55064.1|
                           (AE016861) UDP-3-O-[3-hydroxymyristoyl]  glucosamine N-acyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           353
                           75.63
                           86.96
                           2
                           1410.0
     

     
     
104939
                           gb|AAO90157.1|
                           (AE016961) UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase [Coxiella burnetii RSA 493]
                           354
                           50.56
                           65.25
                           13
                           895.0
     

     






      

          
GC ORFID: 44943How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3646Glimmer Score: 

          
Start: 3480432Stop: 3479371

          
Length: 1062


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA3646 translation of PA3646Homolog Bit Score 688.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4629 (ORF: BLASTX 540.0), t_PA3646 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATGAGTACCTTGTCCTACACCCTGGGCCAGCTGGCCGCGCATGTCGGCGCGGAACTCCGTGGCGACGCG 
GACCTGCCGATCCAGGGCCTGGCGACCTTGCAGGAGGCCGGACCGGCTCAGCTGAGCTTCCTGGCCAACCCG 
CAGTATCGCAAGTACCTGCCCGAAAGCCGTGCCGGCGCCGTCCTGCTGACGGCTGCCGACGCCGACGGGTTC 
TCCGGTACCGCGCTGGTGGTGGCCAATCCCTACCTGGCCTATGCCAGCCTGTCGCACCTGTTCGATCGCAAA 
CCGAAGGCGGCCGCCGGTATCCATCCCACCGCCATCGTCGCGGCGGACGCCGAGGTCGATCCGAGCGCCAGC 
GTCGGCGCCTATGCGGTGATCGAGAGTGGCGCCCGCATCGGCGCTGGCGTCAGCATCGGCGCGCACTGCGTA 
ATCGGCGCCCGTAGCGTGATCGGCGAAGGCGGCTGGCTGGCGCCGCGGGTCACGCTCTACCACGACGTCACC 
ATCGGCGCGCGGGTGAGTATCCAGTCGGGCGCGGTGATCGGCGGCGAAGGCTTCGGTTTCGCCAACGAGAAG 
GGCGTCTGGCAGAAGATCGCCCAGATCGGCGGGGTGACCATCGGCGACGACGTCGAGATCGGCGCGAACACC 
ACCATCGACCGTGGAGCACTGTCCGATACGCTGATCGGCAACGGTGTGAAGCTGGACAACCAGATCATGATC 
GCGCACAACGTGCAGATCGGCGATCATACCGCCATGGCCGCTTGCGTCGGCATTTCCGGCAGCGCCAAGATC 
GGCAGGCATTGCATGCTCGCCGGCGGCGTTGGCCTGGTCGGCCATATCGAGATCTGCGACAACGTTTTCGTG 
ACCGGCATGACCATGGTGACCCGCTCGATCACCGAGCCGGGATCGTATTCCTCCGGTACCGCCATGCAACCG 
GCGGCCGAATGGAAAAAAAGCGCCGCGCGCATTCGTCAACTGGACGACATGGCGCGTCGACTTCAGCAGCTG 
GAAAAACGCCTGGCTGCCGTGACCTCAAGCGGTGACGCTTCATCTGATGCGTAA