ViewGene
Locus Name:
GeneID:
Location:

GeneID:194
TrivialName: PA14_40640
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_40640
PAO1 Ortholog Locus:PA1846
Sequence Length:2289
Protein Length:762
Start: 3629423
Stop: 3627135
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05235.1|AE004610_8 (AE004610) cis/trans isomerase [Pseudomonas aeruginosa PAO1]
Identities = 757/762 (99%), Positives = 759/762 (99%)
Sequence:
ATGTTGCCAAGACCGTTGGTTGGGTTGGCCTTCGTTTTGTCGTCTTTTATCCAGTCTGTTTCCGCTGCTGAA
ATTTCCTATAGCCGTGACGTCCAGCCGATCTTTACCGCCAAATGCGTCGCCTGCCACGCCTGCTACGATTCG
CCCTGCCAGCTCAACCTGAGCAGCGCCGAGGGCGCGCAGCGCGGCGCCAACCAACTGCCGGTCTACGACGGC
ACGCGGACCAAGGCGCAGGAAACCACCCGCCTGTACCTCGATGCGCACGGTGCCGACGCCTGGCGGCGCAAG
GACTTCTGGTCGGTGCTCGAATCGCAGGATGGCCAGGCCGCACTGATGGCGCGGATGCTCGAGCTTGGCCAC
AGCCAGCCGTTGCAGCCGAATGCGAAGATCCCCGAAGGCCTGGACATTTCGATCAACCGCGCCAACCAGTGC
CCGACGCCGGCCAGCATCGATGCGTTCATCCGCAAGAACCCGGGTTCCGGCATGCCTTTCGCGGTGGCCGGG
CTGAGCGACGACGAATACGCCACCTTGCAGAAGTGGCTGGCCGCGGGCGCCCCGGTCGACCAGCAGCCGTTG
CGGCCGACCGCCGCCGAGGCGCGCCAGGTGGCCAGCTGGGAGCGTTTCCTCAACCAGCCTGGGGCCAAGCAG
AGCCTGGTCTCGCGCTGGCTCTACGAGCACCTGTTCCTGGCGCACCTGTATTTCCCGGAACAGGGCGCGCCC
GGCCACTTCTTCCAGCTGGTGCGTTCACGCACGCCCAGCGGCCAGCCGATCGACCCGATCCCGACCCGGCGT
CCCAACGACGATCCGGGCAACAGCTTCTATTACCGCCTCTGGCCGATCCAGGGCGTGATCGTCCACAAGACG
CACATCACCTATCCGCTGACGGCGAAGAAGCTGGAACGCGTCCAGGAGCTGTTCTTCGGCACCCAGTGGAAC
ACCGACAAGGTTCCCGGCTACGGCGTGCAGAGCCGCGCCAACCCGTTCGTCACCTTCGCCGCGATCCCGCCA
CGGGCGCGCTACCAGTTCATGCTGGACAACGCCGAGTACTTCACCCGTACCTTCATCCGCGGGCCGGTGTGC
CGTGGACAGATCGCCACCGACGTGATCCGCGACAACTTCTGGGTGGTATTCCAGGACCCCGAGCAGGACCTG
TTCGTCACCGACGCCAACTTCCGCGCGCAGAGCGAGCCGCTGCTGGCCTTGCCGGGGCAGATCGACGAGCTG
AAGAACCTGCTCGGCCTGTGGAGCGCCTACCGGGACAAGCGCAACGAGTACGAAGACCTGCGCCAGGACGTC
TACGCCGACGCGCCGCCGCCGACCTGGAACACGATCTGGCACGGCAACGACAACGCCCTGCTGAGCATCTTC
CGCCAGTTCGACAGCGCCTCGGTGCGCAAGGGCCTGCTTGGCGAGGTACCGCAGACCCTGTGGCTGATGGAC
TACCCGCTGTTCGAGCGAACCTACTACGGGCTGGTGGTGAACTTCGATGTCTTCGGCAACGTCTCGCACCAG
GCGCAGACGCGCCTGTACTTCGACCTGATCCGCAACGGCGCCGAGCAGAACTTCCTCCGCTTGATGCCGGTC
GACGCGCGCCAGCCGTTGCTTGACGACTGGTACCAGAACAGCGGCAAGCTGAAGATGTGGATGGACTACCAG
GCCTTCGACGATGACACGCCGAGCGCGCTGGGATTGCCGGAGAAGCAGCCGAAGAAGGCCTTCGCCGAAGAA
CTGCTGCGTCGCTACGGCGACCTCAATGCGCGCCCCGACCCGATCAACCGCTGCCTGGACGGCAACTGCTAT
CGACCGGGCATCGACCGCGAACTGCAGGACGCCGAGCAGGCCTTCAGTCGCCTGGTCAGCCGGCCGGCGGCC
GGCCTCAAGGTCATAGAGCGCTTCCCCGAGGCGACCATGCTGCGGATACGTACGTCCAGCGGCAAGCGCGAG
ATCTATACCGTGCTGCGCAACCGCGCGCACAGCAATGTCGCCTTCATGCTCGGCGAGTCGCTGCGCTACCAG
CCGGGCCTGGACACCCTGACGATCTACCCCGGCGTGCTGTCCAGCTATCCGAACTTCATGTTCGATCTGCCG
GCGACGGATGCCGAGGCCTTCGTCGGCGCCCTGGAGGCGGCGAAGAGTAGCGAGGACTTCGACAAGGTGGTC
GAACGCTGGGGCGTGCGCCGCAGCAATCCGCAGTTCTGGAGCTACTTCCACGATCTGGAGGCGTACATCCGC
GAAACCGAACCGGTCGAGGCGGGCGCGCTGGACATGAATCGCTACGAGAACCTCTGA
Translation:
MLPRPLVGLAFVLSSFIQSVSAAEISYSRDVQPIFTAKCVACHACYDSPCQLNLSSAEGAQRGANQLPVYDG
TRTKAQETTRLYLDAHGADAWRRKDFWSVLESQDGQAALMARMLELGHSQPLQPNAKIPEGLDISINRANQC
PTPASIDAFIRKNPGSGMPFAVAGLSDDEYATLQKWLAAGAPVDQQPLRPTAAEARQVASWERFLNQPGAKQ
SLVSRWLYEHLFLAHLYFPEQGAPGHFFQLVRSRTPSGQPIDPIPTRRPNDDPGNSFYYRLWPIQGVIVHKT
HITYPLTAKKLERVQELFFGTQWNTDKVPGYGVQSRANPFVTFAAIPPRARYQFMLDNAEYFTRTFIRGPVC
RGQIATDVIRDNFWVVFQDPEQDLFVTDANFRAQSEPLLALPGQIDELKNLLGLWSAYRDKRNEYEDLRQDV
YADAPPPTWNTIWHGNDNALLSIFRQFDSASVRKGLLGEVPQTLWLMDYPLFERTYYGLVVNFDVFGNVSHQ
AQTRLYFDLIRNGAEQNFLRLMPVDARQPLLDDWYQNSGKLKMWMDYQAFDDDTPSALGLPEKQPKKAFAEE
LLRRYGDLNARPDPINRCLDGNCYRPGIDRELQDAEQAFSRLVSRPAAGLKVIERFPEATMLRIRTSSGKRE
IYTVLRNRAHSNVAFMLGESLRYQPGLDTLTIYPGVLSSYPNFMFDLPATDAEAFVGALEAAKSSEDFDKVV
ERWGVRRSNPQFWSYFHDLEAYIRETEPVEAGALDMNRYENL*
AnnotationID:204GeneID:194
AnnotatorUID: diggins
Modification Date/Time: 2005-03-02 05:05:53
Gene Name:cti
Confidence Code:2
GeneProduct:fatty acyl cis-trans isomerase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam06934
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAB41104.1| (AB041884) 9-hexadecenoic acid cis-trans isomerase [Pseudomonas
           psychrophila]
          Length = 765

 Score = 1056 bits (2730), Expect = 0.0
 Identities = 495/738 (67%), Positives = 584/738 (79%), Gaps = 1/738 (0%)

>emb|CAD59690.1| (AJ535703) fatty acyl cis-trans isomerase [Pseudomonas syringae]
          Length = 765

 Score = 1054 bits (2725), Expect = 0.0
 Identities = 496/738 (67%), Positives = 583/738 (78%), Gaps = 1/738 (0%)

>gb|AAN67989.1|AE016431_5 (AE016783) esterified fatty acid cis/trans isomerase [Pseudomonas
           putida KT2440]
          Length = 766

 Score = 1047 bits (2707), Expect = 0.0
 Identities = 491/743 (66%), Positives = 594/743 (79%), Gaps = 2/743 (0%)






 
Structural Features:
pfam06934, CTI, Fatty acid cis/trans isomerase (CTI).
CD-Length = 736 residues, 100.0% aligned
Score = 1276 bits (3303), Expect = 0.0
Identities = 536/736 (72%), Positives = 595/736 (80%)






   
Genomic Context:






   
Comment:


















    ReferenceID:391




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17533




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37089




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:194_cdd.html


    		




 
URL:

















    ReferenceID:37788




  
Author/Investigator(s):
        Holtwick R, Meinhardt F, Keweloh H.





  
Title:
        cis-trans isomerization of unsaturated fatty acids: cloning and sequencing of the cti gene from Pseudomonas putida P8.




  
PubMed:
        9361416


MedLine:
        




    
Source:
Appl Environ Microbiol. 1997 Nov;63(11):4292-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9361416









           
Homologs By Global Alignment 

           

       



     
Gene ID:194
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
334
                           PA1846_tr
                           translation of PA1846
                           762
                           99.34
                           99.60
                           0
                           4037.0
     

     
     
21300
                           gb|AAG05235.1|AE004610_8
                           (AE004610) cis/trans isomerase [Pseudomonas aeruginosa PAO1]
                           762
                           99.34
                           99.60
                           0
                           4037.0
     

     
     
21303
                           emb|CAD59690.1|
                           (AJ535703) fatty acyl cis-trans isomerase [Pseudomonas syringae]
                           767
                           65.84
                           77.57
                           7
                           2723.0
     

     
     
21302
                           gb|AAD41252.1|AF110738_2
                           (AF110738) Cti [Pseudomonas putida]
                           768
                           65.62
                           79.16
                           8
                           2734.0
     

     
     
21306
                           emb|CAA04438.1|
                           (AJ000978) cis/trans isomerase [Pseudomonas putida]
                           768
                           65.10
                           78.12
                           8
                           2701.0
     

     






      

          
GC ORFID: 43196How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1846Glimmer Score: 

          
Start: 1324487Stop: 1326775

          
Length: 2289


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1846 translation of PA1846Homolog Bit Score 1559.0

          
Other Homologs:  t_PA1846 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTTGCCAAGACCGTTGGTTGGGTTGGCCTTCGTTTTGTCGTCTTTTATCCAGTCTGTTTCCGCTGCTGAA 
ATTTCCTATAGCCGTGACGTCCAGCCGATCTTTACCGCCAAATGCGTCGCCTGCCACGCCTGCTACGATTCG 
CCCTGCCAGCTCAACCTGAGCAGCGCCGAGGGCGCGCAGCGCGGCGCCAACCAACTGCCGGTCTACGACGGC 
ACGCGGACCAAGGCGCAGGAAACCACCCGCCTGTACCTCGATGCGCACGGTGCCGACGCCTGGCGGCGCAAG 
GACTTCTGGTCGGTGCTCGAATCGCAGGATGGCCAGGCCGCACTGATGGCGCGGATGCTCGAGCTTGGCCAC 
AGCCAGCCGTTGCAGCCGAATGCGAAGATCCCCGAAGGCCTGGACATTTCGATCAACCGCGCCAACCAGTGC 
CCGACGCCGGCCAGCATCGATGCGTTCATCCGCAAGAACCCGGGTTCCGGCATGCCTTTCGCGGTGGCCGGG 
CTGAGCGACGACGAATACGCCACCTTGCAGAAGTGGCTGGCCGCGGGCGCCCCGGTCGACCAGCAGCCGTTG 
CGGCCGACCGCCGCCGAGGCGCGCCAGGTGGCCAGCTGGGAGCGTTTCCTCAACCAGCCTGGGGCCAAGCAG 
AGCCTGGTCTCGCGCTGGCTCTACGAGCACCTGTTCCTGGCGCACCTGTATTTCCCGGAACAGGGCGCGCCC 
GGCCACTTCTTCCAGCTGGTGCGTTCACGCACGCCCAGCGGCCAGCCGATCGACCCGATCCCGACCCGGCGT 
CCCAACGACGATCCGGGCAACAGCTTCTATTACCGCCTCTGGCCGATCCAGGGCGTGATCGTCCACAAGACG 
CACATCACCTATCCGCTGACGGCGAAGAAGCTGGAACGCGTCCAGGAGCTGTTCTTCGGCACCCAGTGGAAC 
ACCGACAAGGTTCCCGGCTACGGCGTGCAGAGCCGCGCCAACCCGTTCGTCACCTTCGCCGCGATCCCGCCA 
CGGGCGCGCTACCAGTTCATGCTGGACAACGCCGAGTACTTCACCCGTACCTTCATCCGCGGGCCGGTGTGC 
CGTGGACAGATCGCCACCGACGTGATCCGCGACAACTTCTGGGTGGTATTCCAGGACCCCGAGCAGGACCTG 
TTCGTCACCGACGCCAACTTCCGCGCGCAGAGCGAGCCGCTGCTGGCCTTGCCGGGGCAGATCGACGAGCTG 
AAGAACCTGCTCGGCCTGTGGAGCGCCTACCGGGACAAGCGCAACGAGTACGAAGACCTGCGCCAGGACGTC 
TACGCCGACGCGCCGCCGCCGACCTGGAACACGATCTGGCACGGCAACGACAACGCCCTGCTGAGCATCTTC 
CGCCAGTTCGACAGCGCCTCGGTGCGCAAGGGCCTGCTTGGCGAGGTACCGCAGACCCTGTGGCTGATGGAC 
TACCCGCTGTTCGAGCGAACCTACTACGGGCTGGTGGTGAACTTCGATGTCTTCGGCAACGTCTCGCACCAG 
GCGCAGACGCGCCTGTACTTCGACCTGATCCGCAACGGCGCCGAGCAGAACTTCCTCCGCTTGATGCCGGTC 
GACGCGCGCCAGCCGTTGCTTGACGACTGGTACCAGAACAGCGGCAAGCTGAAGATGTGGATGGACTACCAG 
GCCTTCGACGATGACACGCCGAGCGCGCTGGGATTGCCGGAGAAGCAGCCGAAGAAGGCCTTCGCCGAAGAA 
CTGCTGCGTCGCTACGGCGACCTCAATGCGCGCCCCGACCCGATCAACCGCTGCCTGGACGGCAACTGCTAT 
CGACCGGGCATCGACCGCGAACTGCAGGACGCCGAGCAGGCCTTCAGTCGCCTGGTCAGCCGGCCGGCGGCC 
GGCCTCAAGGTCATAGAGCGCTTCCCCGAGGCGACCATGCTGCGGATACGTACGTCCAGCGGCAAGCGCGAG 
ATCTATACCGTGCTGCGCAACCGCGCGCACAGCAATGTCGCCTTCATGCTCGGCGAGTCGCTGCGCTACCAG 
CCGGGCCTGGACACCCTGACGATCTACCCCGGCGTGCTGTCCAGCTATCCGAACTTCATGTTCGATCTGCCG 
GCGACGGATGCCGAGGCCTTCGTCGGCGCCCTGGAGGCGGCGAAGAGTAGCGAGGACTTCGACAAGGTGGTC 
GAACGCTGGGGCGTGCGCCGCAGCAATCCGCAGTTCTGGAGCTACTTCCACGATCTGGAGGCGTACATCCGC 
GAAACCGAACCGGTCGAGGCGGGCGCGCTGGACATGAATCGCTACGAGAACCTCTGA