ViewGene
Locus Name:
GeneID:
Location:

GeneID:1932
TrivialName: PA14_14590
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_14590
PAO1 Ortholog Locus:PA3824
Sequence Length:1044
Protein Length:347
Start: 1244929
Stop: 1245972
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07211.1|AE004799_17 (AE004799) S-adenosylmethionine:trna ribosyltransferase-isomerase [Pseudomonas aeruginosa PAO1]
Identities = 346/347 (99%), Positives = 346/347 (99%)
Sequence:
ATGCGTGTCGCCGATTTTCATTTCGACTTGCCCGAGGCCCTGATCGCCCGTCATCCCCTGCCTGAACGCCGC
GCAAGCCGCCTGTTGGCGCTGGATGGCCCGACGGGAACCCTCGCCCACAGACAATTCGCCGATCTCCTGGAC
TACCTGCGTCCGGGCGACCTGATGGTGTTCAACAATACCCGGGTGATCCCGGCGCGCCTGTTCGGCCAGAAG
GAATCCGGCGGCAAGCTGGAAGTCCTGGTCGAGCGCGTGCTCGACCAGCACCGGGTGCTTGCCCACATACGC
GCCAGCAAGGCGCCCAAGCCCGGCACGCGGATCCTCGTCGAAGGTGGTGGCAGCGCGGAGATGCTGCAACGC
CACGATGCCCTGTTCGAACTCGCTTTCGCCGAGCCTGTGCTGCCTTTGCTGGAGCGGGTCGGGCACATGCCG
TTGCCTCCCTATATCGACCGACCGGACGATGCGGCCGACCGCGAGCGCTACCAGACCGTCTACGCCCAGCGC
GCCGGTGCGGTAGCGGCCCCGACCGCCGGCCTGCACTTCGACGAGGCGCTGCTCGAGGCGATCCGCGCGAAG
GGGGTGGATACCGCCTTCGTTACCCTGCATGTCGGCGCCGGTACCTTCCAGCCGGTGCGTGTCGAGCGTATC
GAGGACCACGTGATGCACCGCGAGTGGCTGGAGGTCGGCCAGGACGTGGTGGACGCGGTTTCCGCCTGCCGG
GCCCGCGGCGGCCGGGTAGTCGCCGTCGGCACCACCAGCGTGCGCTCGCTGGAAAGCGCGGCACGCGACGGT
GAGTTGAAGCCCTTCAGCGGCGACACCGACATCTTCATCTACCCGGGCCGGCCGTTCCATGTGGTCGATGCC
CTGGTCACCAACTTCCACCTGCCCGAATCCACCCTGCTGATGCTGGTCTCGGCCTTCGCCGGCTACCCGGAG
ACCATGGCCGCCTACGCCGCGGCGGTGGCGCAGGGCTATCGCTTCTTCAGTTACGGTGATGCCATGTTCATC
ACCCGCAATCCCGCGCCCCGCGGACCCGAGGACTGA
Translation:
MRVADFHFDLPEALIARHPLPERRASRLLALDGPTGTLAHRQFADLLDYLRPGDLMVFNNTRVIPARLFGQK
ESGGKLEVLVERVLDQHRVLAHIRASKAPKPGTRILVEGGGSAEMLQRHDALFELAFAEPVLPLLERVGHMP
LPPYIDRPDDAADRERYQTVYAQRAGAVAAPTAGLHFDEALLEAIRAKGVDTAFVTLHVGAGTFQPVRVERI
EDHVMHREWLEVGQDVVDAVSACRARGGRVVAVGTTSVRSLESAARDGELKPFSGDTDIFIYPGRPFHVVDA
LVTNFHLPESTLLMLVSAFAGYPETMAAYAAAVAQGYRFFSYGDAMFITRNPAPRGPED*
AnnotationID:1941GeneID:1932
AnnotatorUID: diggins
Modification Date/Time: 2005-04-24 15:03:22
Gene Name:queA
Confidence Code:2
GeneProduct:S-adenosylmethionine:tRNA ribosyltransferase-isomerase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:queuosine biosynthesis
Functional Category Confidence Code:5
COGs:COG0809,pfam02547
Secondary Functional Category(ies):
EC Number:5.-.-.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66457.1|AE016275_2 (AE016777) S-adenosylmethionine:tRNA ribosyltransferase-isomerase
           [Pseudomonas putida KT2440]
          Length = 349

 Score =  612 bits (1577), Expect = e-174
 Identities = 302/347 (87%), Positives = 317/347 (91%)

>gb|AAC73508.1| (AE000147) synthesis of queuine in tRNA; probably
           S-adenosylmethionine:tRNA ribosyltransferase-isomerase
           [Escherichia coli K12]
          Length = 356

 Score =  478 bits (1230), Expect = e-134
 Identities = 246/347 (70%), Positives = 281/347 (80%), Gaps = 7/347 (2%)








 
Structural Features:
COG0809, QueA, S-adenosylmethionine:tRNA-ribosyltransferase-isomerase 
(queuine synthetase) 
[Translation, ribosomal structure and biogenesis]
CD-Length = 348 residues,  99.7% aligned
Score =  535 bits (1381), Expect = 4e-153
Identities = 221/347 (63%), Positives = 262/347 (75%), Gaps = 6/347 (1%)

pfam02547, Queuosine_synth, Queuosine biosynthesis protein.
CD-Length = 217 residues, 100.0% aligned
Score =  285 bits (731), Expect = 1e-77
Identities = 126/217 (58%), Positives = 158/217 (72%), Gaps = 4/217 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3867




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21014




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31733




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1932_cdd.html


    		




 
URL:

















    ReferenceID:34315




  
Author/Investigator(s):
        Reuter K, Slany R, Ullrich F, Kersten H.





  
Title:
        Structure and organization of Escherichia coli genes involved in biosynthesis of the deazaguanine derivative queuine, a nutrient factor for eukaryotes.




  
PubMed:
        1706703


MedLine:
        




    
Source:
J Bacteriol. 1991 Apr;173(7):2256-64.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1706703









           
Homologs By Global Alignment 

           

       



     
Gene ID:1932
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3388
                           PA3824_tr
                           translation of PA3824
                           347
                           99.71
                           99.71
                           0
                           1784.0
     

     
     
104620
                           gb|AAG07211.1|AE004799_17
                           (AE004799) S-adenosylmethionine:trna ribosyltransferase-isomerase [Pseudomonas aeruginosa PAO1]
                           347
                           99.71
                           99.71
                           0
                           1784.0
     

     
     
104623
                           gb|AAN66457.1|AE016275_2
                           (AE016777) S-adenosylmethionine:tRNA ribosyltransferase-isomerase [Pseudomonas putida KT2440]
                           349
                           86.53
                           90.83
                           2
                           1577.0
     

     
     
104624
                           gb|AAF71490.1|AF232005_1
                           (AF232005) QueA [Pseudomonas syringae pv. syringae]
                           354
                           84.74
                           91.24
                           7
                           1577.0
     

     
     
104621
                           gb|AAO54933.1|
                           (AE016860) S-adenosylmethionine:tRNA ribosyltransferase-isomerase [Pseudomonas syringae pv. tomato str. DC3000]
                           354
                           84.46
                           91.52
                           7
                           1580.0
     

     






      

          
GC ORFID: 44934How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3824Glimmer Score: 

          
Start: 3709009Stop: 3707966

          
Length: 1044


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3824 translation of PA3824Homolog Bit Score 691.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4507 (ORF: BLASTX 614.0), t_PA3824 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGTGTCGCCGATTTTCATTTCGACTTGCCCGAGGCCCTGATCGCCCGTCATCCCCTGCCTGAACGCCGC 
GCAAGCCGCCTGTTGGCGCTGGATGGCCCGACGGGAACCCTCGCCCACAGACAATTCGCCGATCTCCTGGAC 
TACCTGCGTCCGGGCGACCTGATGGTGTTCAACAATACCCGGGTGATCCCGGCGCGCCTGTTCGGCCAGAAG 
GAATCCGGCGGCAAGCTGGAAGTCCTGGTCGAGCGCGTGCTCGACCAGCACCGGGTGCTTGCCCACATACGC 
GCCAGCAAGGCGCCCAAGCCCGGCACGCGGATCCTCGTCGAAGGTGGTGGCAGCGCGGAGATGCTGCAACGC 
CACGATGCCCTGTTCGAACTCGCTTTCGCCGAGCCTGTGCTGCCTTTGCTGGAGCGGGTCGGGCACATGCCG 
TTGCCTCCCTATATCGACCGACCGGACGATGCGGCCGACCGCGAGCGCTACCAGACCGTCTACGCCCAGCGC 
GCCGGTGCGGTAGCGGCCCCGACCGCCGGCCTGCACTTCGACGAGGCGCTGCTCGAGGCGATCCGCGCGAAG 
GGGGTGGATACCGCCTTCGTTACCCTGCATGTCGGCGCCGGTACCTTCCAGCCGGTGCGTGTCGAGCGTATC 
GAGGACCACGTGATGCACCGCGAGTGGCTGGAGGTCGGCCAGGACGTGGTGGACGCGGTTTCCGCCTGCCGG 
GCCCGCGGCGGCCGGGTAGTCGCCGTCGGCACCACCAGCGTGCGCTCGCTGGAAAGCGCGGCACGCGACGGT 
GAGTTGAAGCCCTTCAGCGGCGACACCGACATCTTCATCTACCCGGGCCGGCCGTTCCATGTGGTCGATGCC 
CTGGTCACCAACTTCCACCTGCCCGAATCCACCCTGCTGATGCTGGTCTCGGCCTTCGCCGGCTACCCGGAG 
ACCATGGCCGCCTACGCCGCGGCGGTGGCGCAGGGCTATCGCTTCTTCAGTTACGGTGATGCCATGTTCATC 
ACCCGCAATCCCGCGCCCCGCGGACCCGAGGACTGA