ViewGene
Locus Name:
GeneID:
Location:

GeneID:1913
TrivialName: PA14_67670
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_67670
PAO1 Ortholog Locus:PA5124
Sequence Length:1077
Protein Length:358
Start: 6044551
Stop: 6045627
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08509.1|AE004925_7 (AE004925) two-component sensor NtrB [Pseudomonas aeruginosa PAO1]
Identities = 358/358 (100%), Positives = 358/358 (100%)
Sequence:
ATGCCGACCGATACCCTGCACCGACTGTTGCTCGACAACCTGACCACCGCGGTGATCCTGCTCAACGGCGAA
CTGCGCCTGGAGTACATGAACCCGGCAGCGGAGATGCTCCTGGCCGTGAGCGGCCAGCGCAGCCACGGGCAA
TTCATCAGCGAGCTGTTCACCGAATCGCCGGAAGCCTTGAACTCGCTGCGCCAGGCGGTGGAACAGGCGCAT
CCGTTCACCAAGCGCGAAGCGACCCTGACCTCTATCACCGGCGTCAGCATCACGGTCGACTATGCGGTGACG
CCCATCCTCAACCGCAACGAGACGCTGCTGCTGCTCGAGGTCCATCCGCGCGACCGGCTGATGCGGATCACC
CGCGAAGAAGCACAGCTGTCCAAACAGGAGACCACCAAGCTGCTGGTGCGCGGGCTCGCCCACGAGATCAAG
AACCCGCTCGGCGGCATCCGCGGCGCAGCGCAACTACTATCCCGCGAGCTGCCGGAAGAGTCGCTGAAGGAC
TACACCAACGTCATCATCGAAGAAGCCGACCGGTTGCGGAACCTGGTGGACCGCATGCTCGGCTCGAACAAA
CTGCCGAACCTGGCGCCGACCAACATCCATGAAGTGCTGGAGCGGGTCAGCAGCCTGGTGGAAGCGGAGAGC
CAGGGCAGCATCACCCTGGTCCGCGACTACGACCCGAGCATCCCCGACCTGCTGCTCGACCGCGAGCAAATG
ATCCAGGCCGTGCTCAACATGGTACGCAACGCCATGCAGGCGATCGCCGGGCAGAACGACCTGCGCCTCGGT
CGCATCACCCTGCGCAGCCGTACCCTGCGCCAGTTCACCATCGGCCACACGCGTCACCGCCTGGTGTGCAAG
GTGGAGATCATCGACAACGGCCCGGGCATTCCGGCCGAGCTCCAGGAAACCATCTTCTACCCCATGGTCAGC
GGCCGTCCGGACGGTACCGGACTCGGCCTGGCCATCGCCCAGAACATCATCAGCCAGCACCAGGGGCTGATT
GAATGCGAGAGCCACCCCGGCCATACCGTGTTCAGTCTGTTCCTGCCCCTGGAACAAGGAGTGCATTGA
Translation:
MPTDTLHRLLLDNLTTAVILLNGELRLEYMNPAAEMLLAVSGQRSHGQFISELFTESPEALNSLRQAVEQAH
PFTKREATLTSITGVSITVDYAVTPILNRNETLLLLEVHPRDRLMRITREEAQLSKQETTKLLVRGLAHEIK
NPLGGIRGAAQLLSRELPEESLKDYTNVIIEEADRLRNLVDRMLGSNKLPNLAPTNIHEVLERVSSLVEAES
QGSITLVRDYDPSIPDLLLDREQMIQAVLNMVRNAMQAIAGQNDLRLGRITLRSRTLRQFTIGHTRHRLVCK
VEIIDNGPGIPAELQETIFYPMVSGRPDGTGLGLAIAQNIISQHQGLIECESHPGHTVFSLFLPLEQGVH*
AnnotationID:1922GeneID:1913
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 12:12:38
Gene Name:ntrB
Confidence Code:2
GeneProduct:two-component sensor NtrB
Cell Localization:()
Synonyms:glnL
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(28) Two-component regulatory systems
Alternate Gene Product Name:nitrogen regulation protein NtrB
Functional Category Confidence Code:5
COGs:COG3852
Secondary Functional Category(ies):signal transduction mechanism
EC Number:2.7.3.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO53897.1| (AE016857) nitrogen regulation protein NtrB [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 361

 Score =  620 bits (1600), Expect = e-177
 Identities = 310/354 (87%), Positives = 339/354 (95%)

>gb|AAN70612.1|AE016704_3 (AE016793) sensory box histidine kinase NtrB [Pseudomonas putida
           KT2440]
          Length = 361

 Score =  612 bits (1578), Expect = e-174
 Identities = 309/354 (87%), Positives = 334/354 (94%)

>gb|AAA27524.1| (L08499) nitrogen regulator 1 [Vibrio alginolyticus]
          Length = 348

 Score =  323 bits (829), Expect = 7e-88
 Identities = 168/349 (48%), Positives = 241/349 (69%), Gaps = 4/349 (1%)








 
Structural Features:
COG3852, NtrB, Signal transduction histidine kinase, nitrogen specific 
[Signal transduction mechanisms]
CD-Length = 363 residues,  98.3% aligned
Score =  455 bits (1173), Expect = 3e-129
Identities = 195/360 (54%), Positives = 248/360 (68%), Gaps = 5/360 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3829




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20976




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34951




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1913_cdd.html


    		




 
URL:

















    ReferenceID:35796




  
Author/Investigator(s):
        Miranda-Rios J, Sanchez-Pescador R, Urdea M, Covarrubias AA.





  
Title:
        The complete nucleotide sequence of the glnALG operon of Escherichia coli K12.




  
PubMed:
        2882477


MedLine:
        




    
Source:
Nucleic Acids Res. 1987 Mar 25;15(6):2757-70.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2882477









           
Homologs By Global Alignment 

           

       



     
Gene ID:1913
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3367
                           PA5124_tr
                           translation of PA5124
                           358
                           99.99
                           99.99
                           0
                           1796.0
     

     
     
104264
                           gb|AAG08509.1|AE004925_7
                           (AE004925) two-component sensor NtrB [Pseudomonas aeruginosa PAO1]
                           358
                           99.99
                           99.99
                           0
                           1796.0
     

     
     
104265
                           gb|AAO53897.1|
                           (AE016857) nitrogen regulation protein NtrB [Pseudomonas syringae pv. tomato str. DC3000]
                           361
                           85.87
                           93.90
                           3
                           1594.0
     

     
     
104266
                           gb|AAN70612.1|AE016704_3
                           (AE016793) sensory box histidine kinase NtrB [Pseudomonas putida KT2440]
                           361
                           85.59
                           92.52
                           3
                           1572.0
     

     
     
104267
                           emb|CAC79243.1|
                           (AJ320536) nitrogen regulation protein B [Pseudomonas stutzeri]
                           360
                           84.16
                           92.22
                           2
                           1552.0
     

     






      

          
GC ORFID: 44915How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5124Glimmer Score: 

          
Start: 5308236Stop: 5309312

          
Length: 1077


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5124 translation of PA5124Homolog Bit Score 696.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6893 (ORF: BLASTX 623.0), t_PA5124 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCGACCGATACCCTGCACCGACTGTTGCTCGACAACCTGACCACCGCGGTGATCCTGCTCAACGGCGAA 
CTGCGCCTGGAGTACATGAACCCGGCAGCGGAGATGCTCCTGGCCGTGAGCGGCCAGCGCAGCCACGGGCAA 
TTCATCAGCGAGCTGTTCACCGAATCGCCGGAAGCCTTGAACTCGCTGCGCCAGGCGGTGGAACAGGCGCAT 
CCGTTCACCAAGCGCGAAGCGACCCTGACCTCTATCACCGGCGTCAGCATCACGGTCGACTATGCGGTGACG 
CCCATCCTCAACCGCAACGAGACGCTGCTGCTGCTCGAGGTCCATCCGCGCGACCGGCTGATGCGGATCACC 
CGCGAAGAAGCACAGCTGTCCAAACAGGAGACCACCAAGCTGCTGGTGCGCGGGCTCGCCCACGAGATCAAG 
AACCCGCTCGGCGGCATCCGCGGCGCAGCGCAACTACTATCCCGCGAGCTGCCGGAAGAGTCGCTGAAGGAC 
TACACCAACGTCATCATCGAAGAAGCCGACCGGTTGCGGAACCTGGTGGACCGCATGCTCGGCTCGAACAAA 
CTGCCGAACCTGGCGCCGACCAACATCCATGAAGTGCTGGAGCGGGTCAGCAGCCTGGTGGAAGCGGAGAGC 
CAGGGCAGCATCACCCTGGTCCGCGACTACGACCCGAGCATCCCCGACCTGCTGCTCGACCGCGAGCAAATG 
ATCCAGGCCGTGCTCAACATGGTACGCAACGCCATGCAGGCGATCGCCGGGCAGAACGACCTGCGCCTCGGT 
CGCATCACCCTGCGCAGCCGTACCCTGCGCCAGTTCACCATCGGCCACACGCGTCACCGCCTGGTGTGCAAG 
GTGGAGATCATCGACAACGGCCCGGGCATTCCGGCCGAGCTCCAGGAAACCATCTTCTACCCCATGGTCAGC 
GGCCGTCCGGACGGTACCGGACTCGGCCTGGCCATCGCCCAGAACATCATCAGCCAGCACCAGGGGCTGATT 
GAATGCGAGAGCCACCCCGGCCATACCGTGTTCAGTCTGTTCCTGCCCCTGGAACAAGGAGTGCATTGA