ViewGene
Locus Name:
GeneID:
Location:

GeneID:1907
TrivialName: PA14_18710
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18710
PAO1 Ortholog Locus:PA3527
Sequence Length:1047
Protein Length:348
Start: 1611041
Stop: 1609995
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06915.1|AE004773_4 (AE004773) dihydroorotase [Pseudomonas aeruginosa PAO1]
Identities = 346/348 (99%), Positives = 346/348 (99%)
Sequence:
ATGTCCGACCGTCTCACCCTCCTGCGCCCCGACGACTGGCACATTCACCTGCGTGACGGTGCCGCGCTGGCC
AATACCGTCGGCGACGCCGCCCGCACCTTCGGCCGCGCCATCGTCATGCCGAACCTGGTGCCGCCGGTGCGC
AACGCCGCCGAAGCCGACGCCTACCGGCAGCGCATCCTCGCCGCCCGTCCCGCCGCCAGCCGCTTCGAGCCG
CTGATGGTGCTGTACCTCACCGACCGCACCAGCGCCGAGGAAATCCGTACCGCCAAGGCCAGCGGCTTCGTG
CATGCCGCCAAGCTCTATCCGGCAGGCGCCACCACCAACTCGGACTCTGGCGTGACCCGCATCGACAATATC
TTCGAAGCGCTCGAGGCGATGGCCGAGGTCGGCATGCCACTGCTGGTGCATGGCGAGGTGACCCGCGCCGAG
GTCGACGTATTCGATCGCGAGAAGCAGTTCATCGACGAGCACCTGCGCCGGGTGGTCGAGCGCTTCCCGACC
CTGAAAGTGGTCTTCGAGCACATCACCACCGGCGACGCCGCCCAGTTCGTCCGGGAAGCCCCGGCCAACGTC
GGCGCGACCATTACCGCCCATCACCTGCTGTACAACCGCAACCACATGCTGGTCGGCGGTATCCGCCCGCAT
TTCTATTGCCTGCCGATCCTCAAGCGCAACACCCACCAGGAAGCCCTGCTGGACGCCGCGGTGAGCGGCAAC
CCGAAATTCTTCCTCGGCACCGACTCGGCGCCGCACGCCCGCCACGCCAAGGAAACCGCCTGCGGTTGCGCC
GGCTGCTACAGCGCCTACGCCGCCATCGAGCTGTATGCCGAGGCCTTCGAACAGCGCAATGCGCTGGACAAG
CTGGAAGGCTTCGCCAGCCTGCATGGCCCGGATTTCTATGGCCTGCCGCGCAACACCGACCGGATCACCCTG
GTCCGCGAGGAGTGGCAGGCGCCCGCCAGCCTCCCCTTCGGCGACTTCGACGTGGTACCGCTGCGCGCCGGC
GAGACACTCCGCTGGAAACTGCTGGAGGCCGGGGCATGA
Translation:
MSDRLTLLRPDDWHIHLRDGAALANTVGDAARTFGRAIVMPNLVPPVRNAAEADAYRQRILAARPAASRFEP
LMVLYLTDRTSAEEIRTAKASGFVHAAKLYPAGATTNSDSGVTRIDNIFEALEAMAEVGMPLLVHGEVTRAE
VDVFDREKQFIDEHLRRVVERFPTLKVVFEHITTGDAAQFVREAPANVGATITAHHLLYNRNHMLVGGIRPH
FYCLPILKRNTHQEALLDAAVSGNPKFFLGTDSAPHARHAKETACGCAGCYSAYAAIELYAEAFEQRNALDK
LEGFASLHGPDFYGLPRNTDRITLVREEWQAPASLPFGDFDVVPLRAGETLRWKLLEAGA*
AnnotationID:1916GeneID:1907
AnnotatorUID: diggins
Modification Date/Time: 2005-04-19 16:04:07
Gene Name:pyrC
Confidence Code:1
GeneProduct:dihydroorotase, homodimeric type
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0418
Secondary Functional Category(ies):
EC Number:3.5.2.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66711.1|AE016299_7 (AE016778) dihydroorotase, homodimeric type [Pseudomonas putida
           KT2440]
          Length = 348

 Score =  622 bits (1604), Expect = e-177
 Identities = 304/348 (87%), Positives = 320/348 (91%)

>gb|AAN56679.1|AE015804_1 (AE015804) dihydroorotase, homodimeric type [Shewanella oneidensis
           MR-1]
          Length = 343

 Score =  484 bits (1245), Expect = e-136
 Identities = 240/339 (70%), Positives = 271/339 (79%)

>emb|CAA28157.1| (X04469) dihydroorotase [Escherichia coli]
          Length = 348

 Score =  387 bits (994), Expect = e-107
 Identities = 194/342 (56%), Positives = 230/342 (67%)








 
Structural Features:
COG0418, PyrC, Dihydroorotase [Nucleotide transport and metabolism]
CD-Length = 344 residues,  99.7% aligned
Score =  565 bits (1457), Expect = 3e-162
Identities = 229/343 (66%), Positives = 264/343 (76%)








   
Genomic Context:






   
Comment:


















    ReferenceID:3817




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20964




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32036




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1907_cdd.html


    		




 
URL:

















    ReferenceID:34117




  
Author/Investigator(s):
        Brichta DM, Azad KN, Ralli P, O'Donovan GA.





  
Title:
        Pseudomonas aeruginosa dihydroorotases: a tale of three pyrCs.




  
PubMed:
        15278241


MedLine:
        




    
Source:
Arch Microbiol. 2004 Sep;182(1):7-17. Epub 2004 Jul 28.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15278241

















    ReferenceID:34118




  
Author/Investigator(s):
        Schenk-Groninger R, Becker J, Brendel M.





  
Title:
        Cloning, sequencing, and characterizing the Lactobacillus leichmannii pyrC gene encoding dihydroorotase.




  
PubMed:
        8589056


MedLine:
        




    
Source:
Biochimie. 1995;77(4):265-72.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8589056

















    ReferenceID:34119




  
Author/Investigator(s):
        Wilson HR, Chan PT, Turnbough CL Jr.





  
Title:
        Nucleotide sequence and expression of the pyrC gene of Escherichia coli K-12.




  
PubMed:
        2885307


MedLine:
        




    
Source:
J Bacteriol. 1987 Jul;169(7):3051-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2885307

















    ReferenceID:34120




  
Author/Investigator(s):
        Backstrom D, Sjoberg RM, Lundberg LG.





  
Title:
        Nucleotide sequence of the structural gene for dihydroorotase of Escherichia coli K12.




  
PubMed:
        2876892


MedLine:
        




    
Source:
Eur J Biochem. 1986 Oct 1;160(1):77-82.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2876892









           
Homologs By Global Alignment 

           

       



     
Gene ID:1907
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3360
                           PA3527_tr
                           translation of PA3527
                           348
                           99.42
                           99.42
                           0
                           1801.0
     

     
     
104164
                           gb|AAG06915.1|AE004773_4
                           (AE004773) dihydroorotase [Pseudomonas aeruginosa PAO1]
                           348
                           99.42
                           99.42
                           0
                           1801.0
     

     
     
104165
                           gb|AAC73109.1|
                           (U73505) dihydroorotase [Pseudomonas aeruginosa]
                           348
                           99.42
                           99.42
                           0
                           1801.0
     

     
     
104166
                           gb|AAN66711.1|AE016299_7
                           (AE016778) dihydroorotase, homodimeric type [Pseudomonas putida KT2440]
                           348
                           87.35
                           91.95
                           0
                           1604.0
     

     
     
104167
                           gb|AAO57613.1|
                           (AE016870) dihydroorotase, homodimeric type [Pseudomonas syringae pv. tomato str. DC3000]
                           348
                           86.49
                           90.51
                           1
                           1575.0
     

     






      

          
GC ORFID: 44909How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3527Glimmer Score: 

          
Start: 3342893Stop: 3343939

          
Length: 1047


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3527 translation of PA3527Homolog Bit Score 698.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6117 (ORF: BLASTX 606.0), t_PA3527 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCGACCGTCTCACCCTCCTGCGCCCCGACGACTGGCACATTCACCTGCGTGACGGTGCCGCGCTGGCC 
AATACCGTCGGCGACGCCGCCCGCACCTTCGGCCGCGCCATCGTCATGCCGAACCTGGTGCCGCCGGTGCGC 
AACGCCGCCGAAGCCGACGCCTACCGGCAGCGCATCCTCGCCGCCCGTCCCGCCGCCAGCCGCTTCGAGCCG 
CTGATGGTGCTGTACCTCACCGACCGCACCAGCGCCGAGGAAATCCGTACCGCCAAGGCCAGCGGCTTCGTG 
CATGCCGCCAAGCTCTATCCGGCAGGCGCCACCACCAACTCGGACTCTGGCGTGACCCGCATCGACAATATC 
TTCGAAGCGCTCGAGGCGATGGCCGAGGTCGGCATGCCACTGCTGGTGCATGGCGAGGTGACCCGCGCCGAG 
GTCGACGTATTCGATCGCGAGAAGCAGTTCATCGACGAGCACCTGCGCCGGGTGGTCGAGCGCTTCCCGACC 
CTGAAAGTGGTCTTCGAGCACATCACCACCGGCGACGCCGCCCAGTTCGTCCGGGAAGCCCCGGCCAACGTC 
GGCGCGACCATTACCGCCCATCACCTGCTGTACAACCGCAACCACATGCTGGTCGGCGGTATCCGCCCGCAT 
TTCTATTGCCTGCCGATCCTCAAGCGCAACACCCACCAGGAAGCCCTGCTGGACGCCGCGGTGAGCGGCAAC 
CCGAAATTCTTCCTCGGCACCGACTCGGCGCCGCACGCCCGCCACGCCAAGGAAACCGCCTGCGGTTGCGCC 
GGCTGCTACAGCGCCTACGCCGCCATCGAGCTGTATGCCGAGGCCTTCGAACAGCGCAATGCGCTGGACAAG 
CTGGAAGGCTTCGCCAGCCTGCATGGCCCGGATTTCTATGGCCTGCCGCGCAACACCGACCGGATCACCCTG 
GTCCGCGAGGAGTGGCAGGCGCCCGCCAGCCTCCCCTTCGGCGACTTCGACGTGGTACCGCTGCGCGCCGGC 
GAGACACTCCGCTGGAAACTGCTGGAGGCCGGGGCATGA