ViewGene
Locus Name:
GeneID:
Location:

GeneID:1906
TrivialName: PA14_06500
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_06500
PAO1 Ortholog Locus:PA0500
Sequence Length:1059
Protein Length:352
Start: 569617
Stop: 570675
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03889.1|AE004487_3 (AE004487) biotin synthase [Pseudomonas aeruginosa PAO1]
Identities = 351/352 (99%), Positives = 352/352 (100%)
Sequence:
ATGAGTGCAACCGCTTCCGTCGCCACCCGTCACGACTGGTCCCTCGCCGAAGTCCGTGCCCTGTTCGAGCAG
CCGTTCAACGACCTGCTGTTCCAGGCGCAGACGGTGCATCGCGCGTACTTCGACCCGAACCGCGTACAGGTC
TCGACGCTGCTGTCGATCAAGACCGGCGCCTGTCCCGAGGACTGCAAGTACTGCCCGCAGTCCGGCCACTAC
AACACCGGCCTGGACAAGGAGAAGCTGATGGAGGTGCAGAAGGTCCTCGAGGCGGCGGCCGAGGCCAAGGCC
ATCGGTTCGACCCGCTTCTGCATGGGCGCTGCCTGGAAGCACCCGTCGGCCAAGGACATGCCCTACGTCCTG
GAGATGGTCAAGGGCGTGAAGAAGCTCGGCCTGGAGACCTGCATGACCCTCGGCCGCCTGACCCAGGAACAG
ACCCAGGCGCTGGCGGACGCCGGCCTCGACTACTACAACCACAACCTGGATACCTCGCCGGAGTTCTACGGC
AACATCATCACCACCCGCACCTACAGCGAGCGCCTGCAGACCCTGGCCTACGTGCGCGAGGCGGGGATGAAG
ATCTGCTCCGGCGGCATCCTCGGCATGGGCGAGTCGGTGGACGACCGCGCCGGCCTGCTGATCCAGTTGGCG
AACCTGCCGGAGCACCCGGAGTCGGTGCCGATCAACATGCTGGTGAAGGTCAAGGGCACGCCCCTGGCGGAA
GAGAAGGACGTCGATCCCTTCGACTTCATCCGCACCCTGGCGGTGGCCCGGATCATGATGCCGAAGTCCCAC
GTGCGCCTGTCCGCCGGCCGCGAGCAGATGAACGAGCAGATGCAGGCGCTGGCCTTCATGGCCGGGGCCAAC
TCGATCTTTTACGGCGAAAAGCTGCTGACCACGAAGAACCCGCAGGCCGAAAAGGACATGCAGTTGTTCGCC
CGTCTCGGCATCAAGCCGGAAGAGCGCGAAGAGCACGCCGACGAAGTGCACCAGGCCGCCATCGAGCAGGCG
TTGGTGGAACAACGCGAATCGAAGCTGTTCTATAACGCCGCTTCCGCCTGA
Translation:
MSATASVATRHDWSLAEVRALFEQPFNDLLFQAQTVHRAYFDPNRVQVSTLLSIKTGACPEDCKYCPQSGHY
NTGLDKEKLMEVQKVLEAAAEAKAIGSTRFCMGAAWKHPSAKDMPYVLEMVKGVKKLGLETCMTLGRLTQEQ
TQALADAGLDYYNHNLDTSPEFYGNIITTRTYSERLQTLAYVREAGMKICSGGILGMGESVDDRAGLLIQLA
NLPEHPESVPINMLVKVKGTPLAEEKDVDPFDFIRTLAVARIMMPKSHVRLSAGREQMNEQMQALAFMAGAN
SIFYGEKLLTTKNPQAEKDMQLFARLGIKPEEREEHADEVHQAAIEQALVEQRESKLFYNAASA*
AnnotationID:1915GeneID:1906
AnnotatorUID: diggins
Modification Date/Time: 2005-03-28 04:04:26
Gene Name:bioB
Confidence Code:2
GeneProduct:biotin synthase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0502
Secondary Functional Category(ies):
EC Number:2.8.1.6
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN65993.1|AE016228_6 (AE016775) biotin synthetase [Pseudomonas putida KT2440]
          Length = 352

 Score =  647 bits (1669), Expect = 0.0
 Identities = 318/352 (90%), Positives = 339/352 (96%)

>gb|AAO54038.1| (AE016857) biotin synthetase [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 352

 Score =  615 bits (1586), Expect = e-175
 Identities = 304/352 (86%), Positives = 326/352 (92%)

>gb|AAF94271.1| (AE004192) biotin synthase [Vibrio cholerae]
          Length = 350

 Score =  491 bits (1263), Expect = e-138
 Identities = 240/346 (69%), Positives = 283/346 (81%), Gaps = 5/346 (1%)








 
Structural Features:
COG0502, BioB, Biotin synthase and related enzymes [Coenzyme metabolism]
CD-Length = 335 residues,  94.3% aligned
Score =  438 bits (1129), Expect = 3e-124
Identities = 191/318 (60%), Positives = 240/318 (75%), Gaps = 4/318 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3815




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20962




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33808




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1906_cdd.html


    		




 
URL:

















    ReferenceID:35328




  
Author/Investigator(s):
        Otsuka AJ, Buoncristiani MR, Howard PK, Flamm J, Johnson C, Yamamoto R, Uchida K, Cook C, Ruppert J, Matsuzaki J.





  
Title:
        The Escherichia coli biotin biosynthetic enzyme sequences predicted from the nucleotide sequence of the bio operon.




  
PubMed:
        3058702


MedLine:
        




    
Source:
J Biol Chem. 1988 Dec 25;263(36):19577-85.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3058702

















    ReferenceID:35333




  
Author/Investigator(s):
        Kiyasu T, Nagahashi Y, Hoshino T.





  
Title:
        Cloning and characterization of biotin biosynthetic genes of Kurthia sp.




  
PubMed:
        11255013


MedLine:
        




    
Source:
Gene. 2001 Mar 7;265(1-2):103-13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11255013

















    ReferenceID:35334




  
Author/Investigator(s):
        Sakurai N, Akatsuka H, Kawai E, Imai Y, Komatsubara S.





  
Title:
        Complete sequence and organization of the Serratia marcescens biotin operon.




  
PubMed:
        8969526


MedLine:
        




    
Source:
Microbiology. 1996 Nov;142 ( Pt 11):3295-303.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8969526

















    ReferenceID:35335




  
Author/Investigator(s):
        Wu CH, Chen HY, Shiuan D.





  
Title:
        Isolation and characterization of the Erwinia herbicola bio operon and the sequences of the bioA and bioB genes.




  
PubMed:
        8890743


MedLine:
        




    
Source:
Gene. 1996 Oct 3;174(2):251-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8890743









           
Homologs By Global Alignment 

           

       



     
Gene ID:1906
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3359
                           PA0500_tr
                           translation of PA0500
                           352
                           99.71
                           99.99
                           0
                           1805.0
     

     
     
104089
                           gb|AAG03889.1|AE004487_3
                           (AE004487) biotin synthase [Pseudomonas aeruginosa PAO1]
                           352
                           99.71
                           99.99
                           0
                           1805.0
     

     
     
104090
                           gb|AAN65993.1|AE016228_6
                           (AE016775) biotin synthetase [Pseudomonas putida KT2440]
                           352
                           90.34
                           96.30
                           0
                           1669.0
     

     
     
104091
                           gb|AAO54038.1|
                           (AE016857) biotin synthetase [Pseudomonas syringae pv. tomato str. DC3000]
                           352
                           86.36
                           92.61
                           0
                           1586.0
     

     
     
104095
                           gb|AAN79328.1|AE016757_232
                           (AE016757) Biotin synthase [Escherichia coli CFT073]
                           353
                           68.55
                           81.58
                           8
                           1257.0
     

     






      

          
GC ORFID: 44908How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0500Glimmer Score: 

          
Start: 6370959Stop: 6372017

          
Length: 1059


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0500 translation of PA0500Homolog Bit Score 699.0

          
Other Homologs:  t_PA0500 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGTGCAACCGCTTCCGTCGCCACCCGTCACGACTGGTCCCTCGCCGAAGTCCGTGCCCTGTTCGAGCAG 
CCGTTCAACGACCTGCTGTTCCAGGCGCAGACGGTGCATCGCGCGTACTTCGACCCGAACCGCGTACAGGTC 
TCGACGCTGCTGTCGATCAAGACCGGCGCCTGTCCCGAGGACTGCAAGTACTGCCCGCAGTCCGGCCACTAC 
AACACCGGCCTGGACAAGGAGAAGCTGATGGAGGTGCAGAAGGTCCTCGAGGCGGCGGCCGAGGCCAAGGCC 
ATCGGTTCGACCCGCTTCTGCATGGGCGCTGCCTGGAAGCACCCGTCGGCCAAGGACATGCCCTACGTCCTG 
GAGATGGTCAAGGGCGTGAAGAAGCTCGGCCTGGAGACCTGCATGACCCTCGGCCGCCTGACCCAGGAACAG 
ACCCAGGCGCTGGCGGACGCCGGCCTCGACTACTACAACCACAACCTGGATACCTCGCCGGAGTTCTACGGC 
AACATCATCACCACCCGCACCTACAGCGAGCGCCTGCAGACCCTGGCCTACGTGCGCGAGGCGGGGATGAAG 
ATCTGCTCCGGCGGCATCCTCGGCATGGGCGAGTCGGTGGACGACCGCGCCGGCCTGCTGATCCAGTTGGCG 
AACCTGCCGGAGCACCCGGAGTCGGTGCCGATCAACATGCTGGTGAAGGTCAAGGGCACGCCCCTGGCGGAA 
GAGAAGGACGTCGATCCCTTCGACTTCATCCGCACCCTGGCGGTGGCCCGGATCATGATGCCGAAGTCCCAC 
GTGCGCCTGTCCGCCGGCCGCGAGCAGATGAACGAGCAGATGCAGGCGCTGGCCTTCATGGCCGGGGCCAAC 
TCGATCTTTTACGGCGAAAAGCTGCTGACCACGAAGAACCCGCAGGCCGAAAAGGACATGCAGTTGTTCGCC 
CGTCTCGGCATCAAGCCGGAAGAGCGCGAAGAGCACGCCGACGAAGTGCACCAGGCCGCCATCGAGCAGGCG 
TTGGTGGAACAACGCGAATCGAAGCTGTTCTATAACGCCGCTTCCGCCTGA