ViewGene
Locus Name:
GeneID:
Location:

GeneID:1899
TrivialName: PA14_57340
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57340
PAO1 Ortholog Locus:PA4412
Sequence Length:1074
Protein Length:357
Start: 5109699
Stop: 5108626
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07800.1|AE004856_11 (AE004856) UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase [Pseudomonas aeruginosa PAO1]
Identities = 357/357 (100%), Positives = 357/357 (100%)
Sequence:
ATGAAAGGTAATGTCCTGATCATGGCGGGTGGCACCGGCGGACACGTGTTCCCGGCGCTCGCCTGTGCGCGG
GAGTTCCAGGCGCGCGGCTATGCCGTGCACTGGCTGGGGACGCCGCGCGGCATCGAGAATGACCTGGTACCC
AAGGCCGGCCTGCCGTTGCACCTGATCCAGGTCAGCGGGCTGCGCGGCAAGGGCCTGAAGTCGCTGGTCAAG
GCGCCGCTGGAACTGCTCAAGTCGCTGTTCCAGGCGCTGCGGGTGATCCGCCAGCTGAGGCCGGTCTGCGTG
CTCGGCCTGGGTGGCTATGTCACCGGCCCGGGCGGCCTGGCCGCGCGCCTCAATGGCGTGCCGCTGGTGATC
CACGAGCAGAACGCCGTGGCCGGCACCGCCAACCGCAGCCTGGCGCCGATCGCCAGGCGCGTCTGCGAGGCA
TTCCCGGATACCTTCCCGGCCAGCGACAAGCGTCTGACCACCGGCAATCCGGTGCGCGGCGAGCTGTTCCTC
GACGCGCACGCCCGCGCGCCGCTGACCGGCCGTCGGGTCAATCTGCTGGTGCTCGGCGGCAGCCTTGGCGCG
GAACCGCTGAACAAGCTGTTGCCAGAGGCCCTCGCGCAGGTGCCGCTGGAAATCCGTCCGGCAATCCGCCAT
CAGGCCGGTCGTCAGCATGCCGAGATTACCGCCGAGCGTTATCGCACAGTGGCGGTCGAAGCGGACGTCGCG
CCCTTCATCAGCGATATGGCCGCGGCCTATGCCTGGGCCGACCTGGTGATCTGCCGCGCCGGCGCGCTGACC
GTCAGCGAGCTGACGGCGGCGGGATTACCCGCCTTCCTGGTGCCGTTGCCTCACGCGATCGACGATCACCAG
ACCCGCAATGCCGAATTCCTGGTACGCAGCGGCGCCGGCCGCCTGCTGCCGCAAAAGTCTACCGGCGCGGCC
GAACTGGCCGCGCAGCTGTCCGAGGTCCTGATGCATCCCGAAACCCTGCGCTCCATGGCAGACCAGGCACGC
AGCCTGGCGAAACCCGAGGCTACCCGGACGGTGGTCGATGCCTGCCTGGAGGTGGCCCGTGGTTAA
Translation:
MKGNVLIMAGGTGGHVFPALACAREFQARGYAVHWLGTPRGIENDLVPKAGLPLHLIQVSGLRGKGLKSLVK
APLELLKSLFQALRVIRQLRPVCVLGLGGYVTGPGGLAARLNGVPLVIHEQNAVAGTANRSLAPIARRVCEA
FPDTFPASDKRLTTGNPVRGELFLDAHARAPLTGRRVNLLVLGGSLGAEPLNKLLPEALAQVPLEIRPAIRH
QAGRQHAEITAERYRTVAVEADVAPFISDMAAAYAWADLVICRAGALTVSELTAAGLPAFLVPLPHAIDDHQ
TRNAEFLVRSGAGRLLPQKSTGAAELAAQLSEVLMHPETLRSMADQARSLAKPEATRTVVDACLEVARG*
AnnotationID:1908GeneID:1899
AnnotatorUID: diggins
Modification Date/Time: 2005-05-02 18:06:25
Gene Name:murG
Confidence Code:2
GeneProduct:UDP-N-acetylglucosamine--N-acetylmuramyl-(pentape
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:UDP-N-acetylglucosamine:LPS N-acetylglucosamine transferase
Functional Category Confidence Code:5
COGs:COG0707,pfam04101
Secondary Functional Category(ies):
EC Number:2.4.1.227
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66960.1|AE016324_10 (AE016779) UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide)
           pyrophosphoryl-undecaprenol N-acetylglucosamine
           transferase [Pseudomonas putida KT2440]
          Length = 359

 Score =  531 bits (1369), Expect = e-150
 Identities = 268/354 (75%), Positives = 297/354 (83%), Gaps = 1/354 (0%)

>gb|AAO57857.1| (AE016871) UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide)
           pyrophosphoryl-undecaprenol N-acetylglucosamine
           transferase [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 356

 Score =  531 bits (1367), Expect = e-150
 Identities = 263/357 (73%), Positives = 303/357 (84%), Gaps = 1/357 (0%)






 
Structural Features:
COG0707, MurG, UDP-N-acetylglucosamine:
LPS N-acetylglucosamine transferase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 357 residues,  99.4% aligned
Score =  351 bits (902), Expect = 1e-97
Identities = 161/358 (44%), Positives = 220/358 (61%), Gaps = 8/358 (2%)

pfam04101, Glyco_tran_28_C, Glycosyltransferase family 28 C-terminal domain.
CD-Length = 166 residues,  99.4% aligned
Score =  133 bits (336), Expect = 7e-32
Identities = 62/165 (37%), Positives = 89/165 (53%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3801




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20948




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:29549




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1899_cdd.html


    		




 
URL:

















    ReferenceID:31598




  
Author/Investigator(s):
        Azzolina BA, Yuan X, Anderson MS, El-Sherbeini M.





  
Title:
        The cell wall and cell division gene cluster in the Mra operon of Pseudomonas aeruginosa: cloning, production, and purification of active enzymes.




  
PubMed:
        11281713


MedLine:
        




    
Source:
Protein Expr Purif. 2001 Apr;21(3):393-400.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11281713









           
Homologs By Global Alignment 

           

       



     
Gene ID:1899
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3349
                           PA4412_tr
                           translation of PA4412
                           357
                           99.99
                           99.99
                           0
                           1807.0
     

     
     
103947
                           gb|AAG07800.1|AE004856_11
                           (AE004856) UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase [Pseudomonas aeruginosa PAO1]
                           357
                           99.99
                           99.99
                           0
                           1807.0
     

     
     
103948
                           gb|AAN66960.1|AE016324_10
                           (AE016779) UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase [Pseudomonas putida KT2440]
                           360
                           74.72
                           83.05
                           4
                           1367.0
     

     
     
103949
                           gb|AAO57857.1|
                           (AE016871) UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase [Pseudomonas syringae pv. tomato str. DC3000]
                           357
                           73.66
                           84.87
                           1
                           1369.0
     

     
     
103950
                           emb|CAD16551.1|
                           (AL646072) PROBABLE UDP-N-ACETYLGLUCOSAMINE--N-ACETYLMURAMYL-PENTAPEPTIDE PYROPHOSPHORYL-UNDECAPRENOL N-ACETYLGLUCOSAMINE TRANSFERASE PROTEIN [Ralstonia solanacearum]
                           370
                           48.10
                           62.16
                           18
                           851.0
     

     






      

          
GC ORFID: 44901How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4412Glimmer Score: 

          
Start: 4373377Stop: 4372304

          
Length: 1074


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4412 translation of PA4412Homolog Bit Score 700.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1128 (ORF: BLASTX 528.0), t_PA4412 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAAGGTAATGTCCTGATCATGGCGGGTGGCACCGGCGGACACGTGTTCCCGGCGCTCGCCTGTGCGCGG 
GAGTTCCAGGCGCGCGGCTATGCCGTGCACTGGCTGGGGACGCCGCGCGGCATCGAGAATGACCTGGTACCC 
AAGGCCGGCCTGCCGTTGCACCTGATCCAGGTCAGCGGGCTGCGCGGCAAGGGCCTGAAGTCGCTGGTCAAG 
GCGCCGCTGGAACTGCTCAAGTCGCTGTTCCAGGCGCTGCGGGTGATCCGCCAGCTGAGGCCGGTCTGCGTG 
CTCGGCCTGGGTGGCTATGTCACCGGCCCGGGCGGCCTGGCCGCGCGCCTCAATGGCGTGCCGCTGGTGATC 
CACGAGCAGAACGCCGTGGCCGGCACCGCCAACCGCAGCCTGGCGCCGATCGCCAGGCGCGTCTGCGAGGCA 
TTCCCGGATACCTTCCCGGCCAGCGACAAGCGTCTGACCACCGGCAATCCGGTGCGCGGCGAGCTGTTCCTC 
GACGCGCACGCCCGCGCGCCGCTGACCGGCCGTCGGGTCAATCTGCTGGTGCTCGGCGGCAGCCTTGGCGCG 
GAACCGCTGAACAAGCTGTTGCCAGAGGCCCTCGCGCAGGTGCCGCTGGAAATCCGTCCGGCAATCCGCCAT 
CAGGCCGGTCGTCAGCATGCCGAGATTACCGCCGAGCGTTATCGCACAGTGGCGGTCGAAGCGGACGTCGCG 
CCCTTCATCAGCGATATGGCCGCGGCCTATGCCTGGGCCGACCTGGTGATCTGCCGCGCCGGCGCGCTGACC 
GTCAGCGAGCTGACGGCGGCGGGATTACCCGCCTTCCTGGTGCCGTTGCCTCACGCGATCGACGATCACCAG 
ACCCGCAATGCCGAATTCCTGGTACGCAGCGGCGCCGGCCGCCTGCTGCCGCAAAAGTCTACCGGCGCGGCC 
GAACTGGCCGCGCAGCTGTCCGAGGTCCTGATGCATCCCGAAACCCTGCGCTCCATGGCAGACCAGGCACGC 
AGCCTGGCGAAACCCGAGGCTACCCGGACGGTGGTCGATGCCTGCCTGGAGGTGGCCCGTGGTTAA