ViewGene
Locus Name:
GeneID:
Location:

GeneID:1896
TrivialName: PA14_23790
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_23790
PAO1 Ortholog Locus:PA3118
Sequence Length:1083
Protein Length:360
Start: 2066538
Stop: 2067620
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06506.1|AE004736_3 (AE004736) 3-isopropylmalate dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 360/360 (100%), Positives = 360/360 (100%)
Sequence:
ATGAGCAAGCAGATCCTGGTTCTCCCCGGTGACGGTATCGGTCCGGAAATCATGGCGGAAGCGGTCAAGGTG
CTGGAGCTGGCCAACGACAGGTTCCAGCTCGGCTTCGAACTGGCCGAGGACGTCATCGGTGGCGCGGCCATC
GACAAGCACGGCGTGCCGCTGGCCGACCAGACCCTGCAGCGCGCGCGCCAGGCCGATGCGGTGCTGCTGGGC
GCGGTAGGAGGGCCGAAATGGGACAGGATCGAACGCGATATCCGTCCCGAGCGCGGTCTGCTGAAGATTCGT
TCGCAACTGGGCCTGTTCGCCAACCTGCGTCCGGCCATCCTCTACCCGCAACTGGCCGCGGCCTCCAGCCTG
AAGCCGGAAGTGGTCGCCGGGCTGGACATCCTCATCGTCCGCGAACTGACCGGCGGCATCTACTTCGGCCAG
CCCCGCGAGCAGCGCGTGCTGGAGAACGGCGAGCGCCAGGCCTACGACACCCTGCCGTACAGCGAGAGCGAA
ATCCGTCGTATCGCCCGCGTCGGTTTCGACATGGCCCGCGTACGCAACAACAGGCTCTGCTCGGTGGACAAG
GCCAACGTCCTCGCCTCCAGCCAACTGTGGCGGGAAGTGGTGGAAGAAGTGGCGAAGGACTACCCGGACGTC
GAGCTTTCCCATATGTACGTGGACAACGCCGCCATGCAGCTGGTGCGCGCGCCCAAGCAGTTCGACGTGATG
GTCACCGACAACATGTTCGGCGACATCCTGTCGGATGAGGCTTCCATGCTGACCGGTTCCATCGGCATGCTG
CCTTCTGCCTCGCTCGACGCGAACAACAAGGGCATGTACGAACCCTGCCACGGTTCCGCCCCGGACATCGCC
GGTCAGGGCATCGCCAACCCGTTGGCGACCATCCTCTCGGTCTCGATGATGCTGCGCTACAGCTTCAGCCAG
GCGACCGCCGCCGACGCCATCGAGCAGGCGGTGAGCAAGGTCCTCGACCAGGGGCTGCGCACCGGCGACATC
TGGTCCGAAGGCTGCCGCAAGGTCGGCACCCGGGAAATGGGCGATGCCGTAGTCGCGGCACTCAAGAATCTG
TAA
Translation:
MSKQILVLPGDGIGPEIMAEAVKVLELANDRFQLGFELAEDVIGGAAIDKHGVPLADQTLQRARQADAVLLG
AVGGPKWDRIERDIRPERGLLKIRSQLGLFANLRPAILYPQLAAASSLKPEVVAGLDILIVRELTGGIYFGQ
PREQRVLENGERQAYDTLPYSESEIRRIARVGFDMARVRNNRLCSVDKANVLASSQLWREVVEEVAKDYPDV
ELSHMYVDNAAMQLVRAPKQFDVMVTDNMFGDILSDEASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDIA
GQGIANPLATILSVSMMLRYSFSQATAADAIEQAVSKVLDQGLRTGDIWSEGCRKVGTREMGDAVVAALKNL
*
AnnotationID:1905GeneID:1896
AnnotatorUID: diggins
Modification Date/Time: 2005-04-13 07:07:18
Gene Name:leuB
Confidence Code:1
GeneProduct: 3-isopropylmalate dehydrogenase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00180,COG0473
Secondary Functional Category(ies):
EC Number:1.1.1.85
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAC45419.1| (U29655) 3-isopropylmalate dehydrogenase [Pseudomonas aeruginosa]
          Length = 360

 Score =  700 bits (1806), Expect = 0.0
 Identities = 359/360 (99%), Positives = 359/360 (99%)

>gb|AAM38299.1| (AE011992) 3-isopropylmalate dehydrogenase [Xanthomonas axonopodis
           pv. citri str. 306]
          Length = 357

 Score =  588 bits (1515), Expect = e-167
 Identities = 297/357 (83%), Positives = 326/357 (91%)

>dbj|BAC65259.1| (AB091435) 3-isopropylmalate dehydrogenase oxidoreductase
           [Burkholderia multivorans]
          Length = 355

 Score =  451 bits (1160), Expect = e-126
 Identities = 234/358 (65%), Positives = 281/358 (78%), Gaps = 8/358 (2%)








 
Structural Features:
pfam00180, Iso_dh, Isocitrate/isopropylmalate dehydrogenase.
CD-Length = 350 residues,  99.7% aligned
Score =  480 bits (1236), Expect = 1e-136
Identities = 215/351 (61%), Positives = 256/351 (72%), Gaps = 4/351 (1%)

COG0473, LeuB, Isocitrate/isopropylmalate dehydrogenase 
[Amino acid transport and metabolism]
CD-Length = 348 residues,  99.7% aligned
Score =  465 bits (1198), Expect = 4e-132
Identities = 205/365 (56%), Positives = 251/365 (68%), Gaps = 23/365 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3795




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20942




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32407




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1896_cdd.html


    		




 
URL:

















    ReferenceID:33601




  
Author/Investigator(s):
        Hoang TT, Schweizer HP.





  
Title:
        Identification and genetic characterization of the Pseudomonas aeruginosa leuB gene encoding 3-isopropylmalate dehydrogenase.




  
PubMed:
        9108278


MedLine:
        




    
Source:
Mol Gen Genet. 1997 Mar 26;254(2):166-70.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9108278









           
Homologs By Global Alignment 

           

       



     
Gene ID:1896
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3344
                           PA3118_tr
                           translation of PA3118
                           360
                           99.99
                           99.99
                           0
                           1810.0
     

     
     
103783
                           gb|AAG06506.1|AE004736_3
                           (AE004736) 3-isopropylmalate dehydrogenase [Pseudomonas aeruginosa PAO1]
                           360
                           99.99
                           99.99
                           0
                           1810.0
     

     
     
103784
                           gb|AAC23938.1|
                           (U93274) LeuB [Pseudomonas aeruginosa]
                           360
                           99.99
                           99.99
                           0
                           1810.0
     

     
     
103785
                           gb|AAC45419.1|
                           (U29655) 3-isopropylmalate dehydrogenase [Pseudomonas aeruginosa]
                           360
                           99.72
                           99.72
                           0
                           1806.0
     

     
     
103786
                           gb|AAN67603.1|AE016390_2
                           (AE016781) 3-isopropylmalate dehydrogenase [Pseudomonas putida KT2440]
                           360
                           91.38
                           96.66
                           0
                           1674.0
     

     






      

          
GC ORFID: 44898How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3118Glimmer Score: 

          
Start: 2887393Stop: 2886311

          
Length: 1083


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA3118 translation of PA3118Homolog Bit Score 701.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene970 (ORF: BLASTX 625.0), t_PA3118 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCAAGCAGATCCTGGTTCTCCCCGGTGACGGTATCGGTCCGGAAATCATGGCGGAAGCGGTCAAGGTG 
CTGGAGCTGGCCAACGACAGGTTCCAGCTCGGCTTCGAACTGGCCGAGGACGTCATCGGTGGCGCGGCCATC 
GACAAGCACGGCGTGCCGCTGGCCGACCAGACCCTGCAGCGCGCGCGCCAGGCCGATGCGGTGCTGCTGGGC 
GCGGTAGGAGGGCCGAAATGGGACAGGATCGAACGCGATATCCGTCCCGAGCGCGGTCTGCTGAAGATTCGT 
TCGCAACTGGGCCTGTTCGCCAACCTGCGTCCGGCCATCCTCTACCCGCAACTGGCCGCGGCCTCCAGCCTG 
AAGCCGGAAGTGGTCGCCGGGCTGGACATCCTCATCGTCCGCGAACTGACCGGCGGCATCTACTTCGGCCAG 
CCCCGCGAGCAGCGCGTGCTGGAGAACGGCGAGCGCCAGGCCTACGACACCCTGCCGTACAGCGAGAGCGAA 
ATCCGTCGTATCGCCCGCGTCGGTTTCGACATGGCCCGCGTACGCAACAACAGGCTCTGCTCGGTGGACAAG 
GCCAACGTCCTCGCCTCCAGCCAACTGTGGCGGGAAGTGGTGGAAGAAGTGGCGAAGGACTACCCGGACGTC 
GAGCTTTCCCATATGTACGTGGACAACGCCGCCATGCAGCTGGTGCGCGCGCCCAAGCAGTTCGACGTGATG 
GTCACCGACAACATGTTCGGCGACATCCTGTCGGATGAGGCTTCCATGCTGACCGGTTCCATCGGCATGCTG 
CCTTCTGCCTCGCTCGACGCGAACAACAAGGGCATGTACGAACCCTGCCACGGTTCCGCCCCGGACATCGCC 
GGTCAGGGCATCGCCAACCCGTTGGCGACCATCCTCTCGGTCTCGATGATGCTGCGCTACAGCTTCAGCCAG 
GCGACCGCCGCCGACGCCATCGAGCAGGCGGTGAGCAAGGTCCTCGACCAGGGGCTGCGCACCGGCGACATC 
TGGTCCGAAGGCTGCCGCAAGGTCGGCACCCGGGAAATGGGCGATGCCGTAGTCGCGGCACTCAAGAATCTG 
TAA