ViewGene
Locus Name:
GeneID:
Location:

GeneID:1883
TrivialName: PA14_46970
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_46970
PAO1 Ortholog Locus:PA1337
Sequence Length:1089
Protein Length:362
Start: 4183014
Stop: 4184102
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04726.1|AE004563_3 (AE004563) glutaminase-asparaginase [Pseudomonas aeruginosa PAO1]
Identities = 361/362 (99%), Positives = 362/362 (100%)
Sequence:
ATGAAGCCATTGCTCCACGCGTTCGCCCCCGGTGTTATGGCCCTGATGCTCCTGCTGCCGCAGGCCGCCCAG
GCCAAGGAAGTCGCACCGCAACAGAAACTGTCCAACGTGGTCATCCTGGCCACCGGCGGTACCATCGCCGGC
GCCGGCGCCAGCGCCGCCAACAGCGCCACCTACACCGCCGCCAAGGTGCCGGTCGACCAGTTGCTGGCCAGC
GTGCCGCAGCTCAAGGACATCGCCAACGTCCGCGGCCAACAGGTGTTCCAGATCGCCTCGGAAAGCTTCACC
AACGAGAACCTGCTGGAACTGGGCAAGACCGTCGCCAAGCTGGCCGATAGCGACGACGTCGACGGCATCGTG
ATCACCCACGGTACCGACACCCTGGAAGAGACTGCCTACTTCCTGACCCTGGTCGAGCACACCGAGAAGCCC
ATCGTGGTGGTCGGCTCGATGCGCCCGGGCACCGCGATGTCCGCCGACGGCATGCTCAACCTGTACAACGCC
GTGGCGGTGGCCGGCGACAAGTCGGCGCGCGGCAAGGGCGTGCTGATCACCATGAACGACGAGATTCTCTCC
GGCCGCGACGCGAGCAAGATGGTCAACATCAAGACCGAAGCGTTCAAGAGCCCGTGGGGCCCGCTGGGCATG
GTGGTCGAGGGTAAGAGCTACTGGTTCCGCGCACCGGTGAAGCGGCACACGGTGAACTCCGAGTTCGACATC
AAGCAGATCTCCGCGCTGGCTCCGGTGGAAATCGCCTACAGCTACGGCAACGTCAGCGACACCGCCTACAAG
GCCCTGGCCCAGGCCGGCGCCAAGGCGATCATCCATGCCGGCACCGGCAACGGCTCGGTGCCCGCGCGCGTG
GTGCCGACCCTGCAGGAACTGCGCAAGCAGGGCGTGCAGATCATCCGCTCCTCGCACGTCAATGCCGGCGGC
TTCGTCCTGCGCAACGCCGAGCAGCCGGACGACAAGAACGACTGGATCGTCGCCCACGACCTGAACCCGCAG
AAGGCGCGCATCCTGGCCGCGGTCGCGATGACCAAGACCCAGGACAGCAAGGAGCTGCAACGGATCTTCTGG
GAATACTGA
Translation:
MKPLLHAFAPGVMALMLLLPQAAQAKEVAPQQKLSNVVILATGGTIAGAGASAANSATYTAAKVPVDQLLAS
VPQLKDIANVRGQQVFQIASESFTNENLLELGKTVAKLADSDDVDGIVITHGTDTLEETAYFLTLVEHTEKP
IVVVGSMRPGTAMSADGMLNLYNAVAVAGDKSARGKGVLITMNDEILSGRDASKMVNIKTEAFKSPWGPLGM
VVEGKSYWFRAPVKRHTVNSEFDIKQISALAPVEIAYSYGNVSDTAYKALAQAGAKAIIHAGTGNGSVPARV
VPTLQELRKQGVQIIRSSHVNAGGFVLRNAEQPDDKNDWIVAHDLNPQKARILAAVAMTKTQDSKELQRIFW
EY*
AnnotationID:1892GeneID:1883
AnnotatorUID: diggins
Modification Date/Time: 2005-04-27 09:09:50
Gene Name:ansB
Confidence Code:2
GeneProduct:glutaminase-asparaginase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:glutaminase-asparaginase precursor
Functional Category Confidence Code:5
COGs:cd00411,pfam00710
Secondary Functional Category(ies):
EC Number:3.5.1.1
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAC33155.1| (AF056495) glutaminase-asparaginase precursor [Pseudomonas
           fluorescens]
          Length = 362

 Score =  591 bits (1523), Expect = e-168
 Identities = 299/362 (82%), Positives = 323/362 (89%)

>gb|AAN68065.1|AE016438_11 (AE016783) L-asparaginase II [Pseudomonas putida KT2440]
          Length = 362

 Score =  586 bits (1510), Expect = e-167
 Identities = 296/362 (81%), Positives = 323/362 (89%)

>gb|AAN81991.1|AE016766_79 (AE016766) L-asparaginase II precursor [Escherichia coli CFT073]
          Length = 348

 Score =  295 bits (755), Expect = 3e-79
 Identities = 159/330 (48%), Positives = 213/330 (64%), Gaps = 5/330 (1%)






 
Structural Features:
cd00411, Asparaginase, Asparaginase (amidohydrolase)
CD-Length = 323 residues, 100.0% aligned
Score =  371 bits (954), Expect = 6e-104
Identities = 153/328 (46%), Positives = 219/328 (66%), Gaps = 8/328 (2%)

pfam00710, Asparaginase
CD-Length = 314 residues, 100.0% aligned
Score =  363 bits (934), Expect = 1e-101
Identities = 163/314 (51%), Positives = 208/314 (66%), Gaps = 1/314 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3769




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20916




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37471




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1883_cdd.html


    		




 
URL:

















    ReferenceID:38009




  
Author/Investigator(s):
        Bonthron DT.





  
Title:
        L-asparaginase II of Escherichia coli K-12: cloning, mapping and sequencing of the ansB gene.




  
PubMed:
        2144836


MedLine:
        




    
Source:
Gene. 1990 Jul 2;91(1):101-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2144836

















    ReferenceID:38010




  
Author/Investigator(s):
        Jennings MP, Beacham IR.





  
Title:
        Analysis of the Escherichia coli gene encoding L-asparaginase II, ansB, and its regulation by cyclic AMP receptor and FNR proteins.




  
PubMed:
        2407723


MedLine:
        




    
Source:
J Bacteriol. 1990 Mar;172(3):1491-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2407723

















    ReferenceID:38011




  
Author/Investigator(s):
        Sun DX, Setlow P.





  
Title:
        Cloning, nucleotide sequence, and expression of the Bacillus subtilis ans operon, which codes for L-asparaginase and L-aspartase.




  
PubMed:
        1711029


MedLine:
        




    
Source:
J Bacteriol. 1991 Jun;173(12):3831-45.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1711029









           
Homologs By Global Alignment 

           

       



     
Gene ID:1883
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3323
                           PA1337_tr
                           translation of PA1337
                           362
                           99.72
                           99.99
                           0
                           1814.0
     

     
     
103030
                           gb|AAG04726.1|AE004563_3
                           (AE004563) glutaminase-asparaginase [Pseudomonas aeruginosa PAO1]
                           362
                           99.72
                           99.99
                           0
                           1814.0
     

     
     
103031
                           gb|AAC33155.1|
                           (AF056495) glutaminase-asparaginase precursor [Pseudomonas fluorescens]
                           362
                           82.59
                           89.22
                           0
                           1523.0
     

     
     
103032
                           gb|AAN68065.1|AE016438_11
                           (AE016783) L-asparaginase II [Pseudomonas putida KT2440]
                           362
                           81.76
                           89.22
                           0
                           1510.0
     

     
     
103033
                           gb|AAB31750.1|
                           glutaminase-asparaginase, PGA=tetrameric bacterial amidohydrolase [Pseudomonas, 7A, Peptide, 337 aa]
                           362
                           75.41
                           82.87
                           25
                           1390.0
     

     






      

          
GC ORFID: 44885How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1337Glimmer Score: 

          
Start: 770895Stop: 769807

          
Length: 1089


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1337 translation of PA1337Homolog Bit Score 703.0

          
Other Homologs:  t_PA1337 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGCCATTGCTCCACGCGTTCGCCCCCGGTGTTATGGCCCTGATGCTCCTGCTGCCGCAGGCCGCCCAG 
GCCAAGGAAGTCGCACCGCAACAGAAACTGTCCAACGTGGTCATCCTGGCCACCGGCGGTACCATCGCCGGC 
GCCGGCGCCAGCGCCGCCAACAGCGCCACCTACACCGCCGCCAAGGTGCCGGTCGACCAGTTGCTGGCCAGC 
GTGCCGCAGCTCAAGGACATCGCCAACGTCCGCGGCCAACAGGTGTTCCAGATCGCCTCGGAAAGCTTCACC 
AACGAGAACCTGCTGGAACTGGGCAAGACCGTCGCCAAGCTGGCCGATAGCGACGACGTCGACGGCATCGTG 
ATCACCCACGGTACCGACACCCTGGAAGAGACTGCCTACTTCCTGACCCTGGTCGAGCACACCGAGAAGCCC 
ATCGTGGTGGTCGGCTCGATGCGCCCGGGCACCGCGATGTCCGCCGACGGCATGCTCAACCTGTACAACGCC 
GTGGCGGTGGCCGGCGACAAGTCGGCGCGCGGCAAGGGCGTGCTGATCACCATGAACGACGAGATTCTCTCC 
GGCCGCGACGCGAGCAAGATGGTCAACATCAAGACCGAAGCGTTCAAGAGCCCGTGGGGCCCGCTGGGCATG 
GTGGTCGAGGGTAAGAGCTACTGGTTCCGCGCACCGGTGAAGCGGCACACGGTGAACTCCGAGTTCGACATC 
AAGCAGATCTCCGCGCTGGCTCCGGTGGAAATCGCCTACAGCTACGGCAACGTCAGCGACACCGCCTACAAG 
GCCCTGGCCCAGGCCGGCGCCAAGGCGATCATCCATGCCGGCACCGGCAACGGCTCGGTGCCCGCGCGCGTG 
GTGCCGACCCTGCAGGAACTGCGCAAGCAGGGCGTGCAGATCATCCGCTCCTCGCACGTCAATGCCGGCGGC 
TTCGTCCTGCGCAACGCCGAGCAGCCGGACGACAAGAACGACTGGATCGTCGCCCACGACCTGAACCCGCAG 
AAGGCGCGCATCCTGGCCGCGGTCGCGATGACCAAGACCCAGGACAGCAAGGAGCTGCAACGGATCTTCTGG 
GAATACTGA