ViewGene
Locus Name:
GeneID:
Location:

GeneID:1880
TrivialName: PA14_17610
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_17610
PAO1 Ortholog Locus:PA3610
Sequence Length:1065
Protein Length:354
Start: 1514089
Stop: 1513025
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06998.1|AE004781_6 (AE004781) polyamine transport protein PotD [Pseudomonas aeruginosa PAO1]
Identities = 352/354 (99%), Positives = 354/354 (100%)
Sequence:
ATGATGAAAAAGCTGTTGCTCGTCGCAACCCTCATGGCAGGAGCCGCCCAGGCCACCGCCGCCGAGAAGCTG
TATCTGTTCAACTGGAATGACTACATCGCCGAGGACACAGTGAAACGCTTCGAGCAGCAATGTGGCTGCGAG
CTGGTGCAGGAGTTCTATTCCGGCACCGAGGAAATGATGGCCAAGCTGGCCGCCGGGGCCAGCGGCTACGAC
GTGATCATCCCGACCCAGAACGCGGTCGAGGCGCTGATCCGCAAGGGCGACCTGCTGGAGCTGGACAAGAGT
CGTCTGGCGAACCTGAGCAACGAGGCCGCGGGCTACCTCGACAAGGATTTCGACAAGGGCAACCGCTATTCC
CTGCCCTACGCCTTCACCACCACCCTGGTCGGCTACAACAAGACCGAGCTGGACAAACTGGGCATCGACCCC
GCCGACTGGTCGGTGATCTTCGACCCGGCGGTCCTGGAGAAGATCAAGGGCAGGGTCACGGTGATGGACGAC
CCGCAGGAACTGTTCGGCGCCGCCCTGAAGTACCTCGGCCACTCCGCCAACGACACCGACCCGCAGCACTGG
AAGGAAGCCCAGGCGCTGATCCTCGCGGCCAAGCCGTACTGGGCCGCGTTCAACTCGTCGAGCTATATCAAG
GAGCTGACCCTGGGCAACATCTGGGTCGCCCATGGCTACTCCAGCGACATGTACCAGGCcAGGGCCGACGCG
gaggCTGCCGGCCGCGCCTTCAaggTCGACTTCGCCCTGCCCCGCCAGGGCGCGGTACTGGCCATCGACAAC
ATGGTGATCCACAAGGGCTCGAAGAACCCCGACCTGGCCTACCGCTTCATCGACTTCATGCTCGACGGTCGG
AACGCTTCCGAGCTGACCAACCAGATCGGTACCGGCACGCCCAACGCCGCCGCCCTGCCCTTCATCAGGCCG
GAAATCAAGACGCTCGCCGCCCTGTTCCCGGACGCCACCACCCAGGCCAGGCTGGAGCCGCTGAAGGACCTG
AACTCGCGCCAGCGACGCGCCCTGAACAAGCTCTGGACCGAGATCAAGCTGCGCTGA
Translation:
MMKKLLLVATLMAGAAQATAAEKLYLFNWNDYIAEDTVKRFEQQCGCELVQEFYSGTEEMMAKLAAGASGYD
VIIPTQNAVEALIRKGDLLELDKSRLANLSNEAAGYLDKDFDKGNRYSLPYAFTTTLVGYNKTELDKLGIDP
ADWSVIFDPAVLEKIKGRVTVMDDPQELFGAALKYLGHSANDTDPQHWKEAQALILAAKPYWAAFNSSSYIK
ELTLGNIWVAHGYSSDMYQARADAEAAGRAFKVDFALPRQGAVLAIDNMVIHKGSKNPDLAYRFIDFMLDGR
NASELTNQIGTGTPNAAALPFIRPEIKTLAALFPDATTQARLEPLKDLNSRQRRALNKLWTEIKLR*
AnnotationID:1889GeneID:1880
AnnotatorUID: diggins
Modification Date/Time: 2005-04-20 13:01:18
Gene Name:potD
Confidence Code:2
GeneProduct:polyamine ABC transporter protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0687
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAD85784.1| (BX321862) Bacterial extracellular solute-binding protein, family 1
           [Nitrosomonas europaea ATCC 19718]
          Length = 369

 Score =  414 bits (1064), Expect = e-115
 Identities = 190/331 (57%), Positives = 258/331 (77%)

>gb|AAD36445.1|AE001791_7 (AE001791) spermidine/putrescine ABC transporter, periplasmic
           spermidine/putrescine-binding protein [Thermotoga
           maritima]
          Length = 336

 Score =  224 bits (570), Expect = 8e-58
 Identities = 138/359 (38%), Positives = 207/359 (57%), Gaps = 33/359 (9%)








 
Structural Features:
COG0687, PotD, Spermidine/putrescine-binding periplasmic protein 
[Amino acid transport and metabolism]
CD-Length = 363 residues,  99.2% aligned
Score =  271 bits (693), Expect = 2e-73
Identities = 144/365 (39%), Positives = 206/365 (56%), Gaps = 18/365 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3763




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20910




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31931




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1880_cdd.html


    		




 
URL:

















    ReferenceID:34166




  
Author/Investigator(s):
        Kashiwagi K, Miyamoto S, Nukui E, Kobayashi H, Igarashi K.





  
Title:
        Functions of potA and potD proteins in spermidine-preferential uptake system in Escherichia coli.




  
PubMed:
        8366082


MedLine:
        




    
Source:
J Biol Chem. 1993 Sep 15;268(26):19358-63.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8366082

















    ReferenceID:34167




  
Author/Investigator(s):
        Igarashi K, Kashiwagi K.





  
Title:
        Polyamine transport in bacteria and yeast.




  
PubMed:
        10585849


MedLine:
        




    
Source:
Biochem J. 1999 Dec 15;344 Pt 3:633-42. 	Related Articles, Links







    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10585849









           
Homologs By Global Alignment 

           

       



     
Gene ID:1880
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3320
                           PA3610_tr
                           translation of PA3610
                           354
                           99.43
                           99.99
                           0
                           1817.0
     

     
     
103021
                           gb|AAG06998.1|AE004781_6
                           (AE004781) polyamine transport protein PotD [Pseudomonas aeruginosa PAO1]
                           354
                           99.43
                           99.99
                           0
                           1817.0
     

     
     
103023
                           gb|AAQ61760.1|
                           (AE016924) polyamine ABC transporter protein [Chromobacterium violaceum ATCC 12472]
                           358
                           55.58
                           75.69
                           8
                           1055.0
     

     
     
103022
                           emb|CAD85784.1|
                           (BX321862) Bacterial extracellular solute-binding protein, family 1 [Nitrosomonas europaea ATCC 19718]
                           370
                           52.70
                           72.97
                           17
                           1065.0
     

     
     
103024
                           gb|AAQ61873.1|
                           (AE016925) polyamine ABC transporter system, substrate-binding protein [Chromobacterium violaceum ATCC 12472]
                           359
                           50.69
                           69.08
                           7
                           944.0
     

     






      

          
GC ORFID: 44882How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3610Glimmer Score: 

          
Start: 3439848Stop: 3440912

          
Length: 1065


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3610 translation of PA3610Homolog Bit Score 704.0

          
Other Homologs:  t_PA3610 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATGAAAAAGCTGTTGCTCGTCGCAACCCTCATGGCAGGAGCCGCCCAGGCCACCGCCGCCGAGAAGCTG 
TATCTGTTCAACTGGAATGACTACATCGCCGAGGACACAGTGAAACGCTTCGAGCAGCAATGTGGCTGCGAG 
CTGGTGCAGGAGTTCTATTCCGGCACCGAGGAAATGATGGCCAAGCTGGCCGCCGGGGCCAGCGGCTACGAC 
GTGATCATCCCGACCCAGAACGCGGTCGAGGCGCTGATCCGCAAGGGCGACCTGCTGGAGCTGGACAAGAGT 
CGTCTGGCGAACCTGAGCAACGAGGCCGCGGGCTACCTCGACAAGGATTTCGACAAGGGCAACCGCTATTCC 
CTGCCCTACGCCTTCACCACCACCCTGGTCGGCTACAACAAGACCGAGCTGGACAAACTGGGCATCGACCCC 
GCCGACTGGTCGGTGATCTTCGACCCGGCGGTCCTGGAGAAGATCAAGGGCAGGGTCACGGTGATGGACGAC 
CCGCAGGAACTGTTCGGCGCCGCCCTGAAGTACCTCGGCCACTCCGCCAACGACACCGACCCGCAGCACTGG 
AAGGAAGCCCAGGCGCTGATCCTCGCGGCCAAGCCGTACTGGGCCGCGTTCAACTCGTCGAGCTATATCAAG 
GAGCTGACCCTGGGCAACATCTGGGTCGCCCATGGCTACTCCAGCGACATGTACCAGGCcAGGGCCGACGCG 
gaggCTGCCGGCCGCGCCTTCAaggTCGACTTCGCCCTGCCCCGCCAGGGCGCGGTACTGGCCATCGACAAC 
ATGGTGATCCACAAGGGCTCGAAGAACCCCGACCTGGCCTACCGCTTCATCGACTTCATGCTCGACGGTCGG 
AACGCTTCCGAGCTGACCAACCAGATCGGTACCGGCACGCCCAACGCCGCCGCCCTGCCCTTCATCAGGCCG 
GAAATCAAGACGCTCGCCGCCCTGTTCCCGGACGCCACCACCCAGGCCAGGCTGGAGCCGCTGAAGGACCTG 
AACTCGCGCCAGCGACGCGCCCTGAACAAGCTCTGGACCGAGATCAAGCTGCGCTGA