ViewGene
Locus Name:
GeneID:
Location:

GeneID:1864
TrivialName: PA14_37190
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_37190
PAO1 Ortholog Locus:PA2118
Sequence Length:1077
Protein Length:358
Start: 3313744
Stop: 3312668
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05506.1|AE004639_4 (AE004639) O6-methylguanine-DNA methyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 357/358 (99%), Positives = 358/358 (100%)
Sequence:
ATGAGCCAGCAGCCCGATCCTTGCAACGCCTACCACACCGACGAACAGCGCTGGGCCGCCGTGCTGGCGCGC
GACGCGGCGGCGGACGGGGCGTTCGTCTATGCGGTGAAGACCACCGGGGTCTATTGCCGTCCGAGTTCCTCG
GCGCGTCGGCCCAGGCGCGAGAACGTCGAGTTCTTCGCCACTGCCGAGGCCGCCGAGGCCGCCGGCTATCGG
CCCAGCCGGCGCGCCGCCGGCGACCGCAGCCTGGCGGCGGAGCAGCGCGCCGAGCGGGTGGCGCAGGCCTGC
CGGATGATCGAGACGGCGGAGACGCCGCCGGCACTGGAGGCGCTGGCCGCCCGACTGGGCATGAGTCCGTTC
CACTTCCATCGCCTGTTCAAGGCCGAAACCGGCCTGACGCCCAAGGCCTACGCCTCGGCCTATCGTGCCCGG
CGCCTGCGCGAGCGCCTGGGCCAGGCATCCGCCTCGGTGACCGAGGCGATCTACGAGTCCGGTTTCAACTCC
AACAGCCGTTTCTACGAGTCCTCGTCGCAACGCCTCGGCATGCGCCCGCGCGATTATCGCGATGGCGGCGCC
GGCGCGGCGATCCGCTTCGCCATCGGCCAGTGCTCGCTGGGCGCGATCCTGGTGGCGCAGAGCCAGCGCGGC
ATCTGCGCGATCCTCCTCGGCGAGGAGCCGGAGCCATTGCTGCGCGAGTTGCAGGACCAGTTCCCGCGCGCC
CAGCTACTCGGCGGCGACGCCGACTTCGAACGGCTGGTGGCGCAGGTGGTGGGCTTCGTCGAGTCGCCTCAA
CTGGGGCTGGACCTGCCGCTGGACGTGCGCGGTACGGCGTTCCAGGAACGGGTCTGGCAGGCGCTGCGGGAA
ATACCGCCGGGCAGCACGGCGAGCTATGCGCAGGTCGCCGAACGGATCGGCGCGCCGCGAGCGGTGCGCGCG
GTGGCGCAGGCCTGCGCGGCGAACCGGATCGCGGTGGCGATTCCCTGCCACCGGGTGGTACGTCGCGACGGC
GACATTTCCGGCTACCGCTGGGGCGTGGAGCGCAAGCGCGAACTGCTGCGCAGGGAAGAGCGGGATTGA
Translation:
MSQQPDPCNAYHTDEQRWAAVLARDAAADGAFVYAVKTTGVYCRPSSSARRPRRENVEFFATAEAAEAAGYR
PSRRAAGDRSLAAEQRAERVAQACRMIETAETPPALEALAARLGMSPFHFHRLFKAETGLTPKAYASAYRAR
RLRERLGQASASVTEAIYESGFNSNSRFYESSSQRLGMRPRDYRDGGAGAAIRFAIGQCSLGAILVAQSQRG
ICAILLGEEPEPLLRELQDQFPRAQLLGGDADFERLVAQVVGFVESPQLGLDLPLDVRGTAFQERVWQALRE
IPPGSTASYAQVAERIGAPRAVRAVAQACAANRIAVAIPCHRVVRRDGDISGYRWGVERKRELLRREERD*
AnnotationID:1873GeneID:1864
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 17:05:30
Gene Name:ada
Confidence Code:2
GeneProduct:O6-methylguanine-DNA methyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name:adaptive response regulator protein
Functional Category Confidence Code:5
COGs:COG2169, COG0350
Secondary Functional Category(ies):transcriptional regulator
EC Number:2.1.1.63
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAE31732.1| (BX640440) O6-methylguanine-DNA methyltransferase [Bordetella
           bronchiseptica]
          Length = 358

 Score =  532 bits (1370), Expect = e-150
 Identities = 270/349 (77%), Positives = 299/349 (85%), Gaps = 1/349 (0%)

>emb|CAC88938.1| (AJ414141) bifunctional regulatory protein/DNA repair protein
           [Yersinia pestis CO92]
          Length = 354

 Score =  449 bits (1155), Expect = e-125
 Identities = 220/341 (64%), Positives = 274/341 (80%), Gaps = 1/341 (0%)

>gb|AAG57348.1|AE005453_5 (AE005453) O6-methylguanine-DNA methyltransferase; transcription
           activator/repressor [Escherichia coli O157:H7 EDL933]
          Length = 354

 Score =  382 bits (982), Expect = e-105
 Identities = 189/343 (55%), Positives = 243/343 (70%), Gaps = 2/343 (0%)






 
Structural Features:
COG2169, Ada, Adenosine deaminase [Nucleotide transport and metabolism]
CD-Length = 187 residues, 100.0% aligned
Score =  215 bits (548), Expect = 7e-57
Identities = 98/190 (51%), Positives = 124/190 (65%), Gaps = 3/190 (1%)

COG0350, Ada, Methylated DNA-protein cysteine methyltransferase 
[DNA replication, recombination, and repair]
CD-Length = 168 residues,  99.4% aligned
Score =  161 bits (409), Expect = 9e-41
Identities = 64/167 (38%), Positives = 89/167 (53%), Gaps = 5/167 (2%)







   
Genomic Context:






   
Comment:


















    ReferenceID:3731




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20878




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32587




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1864_cdd.html


    		




 
URL:

















    ReferenceID:34416




  
Author/Investigator(s):
        Hakura A, Morimoto K, Sofuni T, Nohmi T.





  
Title:
        Cloning and characterization of the Salmonella typhimurium ada gene, which encodes O6-methylguanine-DNA methyltransferase.




  
PubMed:
        1904855


MedLine:
        




    
Source:
J Bacteriol. 1991 Jun;173(12):3663-72.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1904855

















    ReferenceID:34417




  
Author/Investigator(s):
        Morohoshi F, Hayashi K, Munakata N.





  
Title:
        Bacillus subtilis ada operon encodes two DNA alkyltransferases.




  
PubMed:
        2120677


MedLine:
        




    
Source:
Nucleic Acids Res. 1990 Sep 25;18(18):5473-80.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2120677

















    ReferenceID:34418




  
Author/Investigator(s):
        Nakabeppu Y, Kondo H, Kawabata S, Iwanaga S, Sekiguchi M.





  
Title:
        Purification and structure of the intact Ada regulatory protein of Escherichia coli K12, O6-methylguanine-DNA methyltransferase.




  
PubMed:
        2987251


MedLine:
        




    
Source:
J Biol Chem. 1985 Jun 25;260(12):7281-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2987251









           
Homologs By Global Alignment 

           

       



     
Gene ID:1864
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3267
                           PA2118_tr
                           translation of PA2118
                           358
                           99.72
                           99.99
                           0
                           1822.0
     

     
     
101142
                           gb|AAG05506.1|AE004639_4
                           (AE004639) O6-methylguanine-DNA methyltransferase [Pseudomonas aeruginosa PAO1]
                           358
                           99.72
                           99.99
                           0
                           1822.0
     

     
     
101143
                           emb|CAE31732.1|
                           (BX640440) O6-methylguanine-DNA methyltransferase [Bordetella bronchiseptica] [Bordetella bronchiseptica RB50]
                           359
                           75.48
                           83.84
                           2
                           1368.0
     

     
     
101144
                           emb|CAE36320.1|
                           (BX640426) O6-methylguanine-DNA methyltransferase [Bordetella parapertussis]
                           359
                           75.20
                           83.56
                           2
                           1364.0
     

     
     
101145
                           emb|CAD16277.1|
                           (AL646070) PROBABLE ADA REGULATORY OF ADAPTATIVE RESPONSE CONTAINS: METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE EC 2.1.1.63 O-6-METHYLGUANINE-DNA TRANSCRIPTION REGULATOR [Ralstonia solanacearum]
                           361
                           70.36
                           81.44
                           4
                           1311.0
     

     






      

          
GC ORFID: 44866How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2118Glimmer Score: 

          
Start: 1640170Stop: 1641246

          
Length: 1077


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2118 translation of PA2118Homolog Bit Score 706.0

          
Other Homologs:  t_PA2118 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCAGCAGCCCGATCCTTGCAACGCCTACCACACCGACGAACAGCGCTGGGCCGCCGTGCTGGCGCGC 
GACGCGGCGGCGGACGGGGCGTTCGTCTATGCGGTGAAGACCACCGGGGTCTATTGCCGTCCGAGTTCCTCG 
GCGCGTCGGCCCAGGCGCGAGAACGTCGAGTTCTTCGCCACTGCCGAGGCCGCCGAGGCCGCCGGCTATCGG 
CCCAGCCGGCGCGCCGCCGGCGACCGCAGCCTGGCGGCGGAGCAGCGCGCCGAGCGGGTGGCGCAGGCCTGC 
CGGATGATCGAGACGGCGGAGACGCCGCCGGCACTGGAGGCGCTGGCCGCCCGACTGGGCATGAGTCCGTTC 
CACTTCCATCGCCTGTTCAAGGCCGAAACCGGCCTGACGCCCAAGGCCTACGCCTCGGCCTATCGTGCCCGG 
CGCCTGCGCGAGCGCCTGGGCCAGGCATCCGCCTCGGTGACCGAGGCGATCTACGAGTCCGGTTTCAACTCC 
AACAGCCGTTTCTACGAGTCCTCGTCGCAACGCCTCGGCATGCGCCCGCGCGATTATCGCGATGGCGGCGCC 
GGCGCGGCGATCCGCTTCGCCATCGGCCAGTGCTCGCTGGGCGCGATCCTGGTGGCGCAGAGCCAGCGCGGC 
ATCTGCGCGATCCTCCTCGGCGAGGAGCCGGAGCCATTGCTGCGCGAGTTGCAGGACCAGTTCCCGCGCGCC 
CAGCTACTCGGCGGCGACGCCGACTTCGAACGGCTGGTGGCGCAGGTGGTGGGCTTCGTCGAGTCGCCTCAA 
CTGGGGCTGGACCTGCCGCTGGACGTGCGCGGTACGGCGTTCCAGGAACGGGTCTGGCAGGCGCTGCGGGAA 
ATACCGCCGGGCAGCACGGCGAGCTATGCGCAGGTCGCCGAACGGATCGGCGCGCCGCGAGCGGTGCGCGCG 
GTGGCGCAGGCCTGCGCGGCGAACCGGATCGCGGTGGCGATTCCCTGCCACCGGGTGGTACGTCGCGACGGC 
GACATTTCCGGCTACCGCTGGGGCGTGGAGCGCAAGCGCGAACTGCTGCGCAGGGAAGAGCGGGATTGA