ViewGene
Locus Name:
GeneID:
Location:

GeneID:1863
TrivialName: PA14_07230
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_07230
PAO1 Ortholog Locus:PA0555
Sequence Length:1065
Protein Length:354
Start: 623313
Stop: 624377
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03944.1|AE004492_3 (AE004492) fructose-1,6-bisphosphate aldolase [Pseudomonas aeruginosa PAO1]
Identities = 354/354 (100%), Positives = 354/354 (100%)
Sequence:
ATGGCACTCATCAGCATGCGCCAGATGCTGGATCACGCCGCCGAATTCGGCTACGGCGTGCCGGCCTTCAAC
GTCAACAACCTCGAGCAGATGCGCGCCATCATGGAAGCGGCCGACAAGACCGATTCGCCCGTGATCGTCCAG
GCTTCCGCCGGTGCTCGCAAATACGCCGGCGCGCCCTTCCTGCGCCACCTGATCCTCGCCGCGATCGAAGAA
TTCCCGCACATCCCGGTGGTCATGCACCAGGATCACGGCACCAGTCCCGACGTCTGCCAGCGTTCCATCCAG
CTGGGCTTCAGCTCGGTGATGATGGACGGCTCCCTGCGTGAAGACGGCAAGACCCCTGCCGACTACGACTAC
AACGTCCGCGTGACCCAGCAGACCGTGGCGTTCGCCCATGCCTGCGGCGTGTCCGTGGAAGGCGAGCTGGGC
TGCCTGGGTTCCCTGGAAACCGGCATGGCCGGCGAGGAAGACGGCGTCGGCGCGGAAGGCGTGCTGGACCAC
AGCCAGTTGCTGACCGACCCGGAAGAAGCCGCCGACTTCGTGAAGAAGACCAAGGTCGACGCCCTGGCCATC
GCCATCGGCACCAGCCACGGCGCCTACAAGTTCACCAAGCCGCCGACCGGGGACACCCTGTCGATCCAGCGC
ATCAAGGAAATCCACGCGCGCATCCCGGACACCCACCTGGTGATGCACGGCTCTTCCTCGGTACCGCAGGAC
TGGCTGGCGATCATCAACGAGTACGGCGGCGAGATCAAAGAGACCTACGGCGTGCCGGTCGAGGAAATCGTC
GAGGGCATCAAGTACGGCGTGCGCAAGGTGAACATCGACACCGACCTGCGCCTGGCCTCCACCGGTGCCATC
CGCCGCTTCCTGGCGCAGAACCCGAGCGAGTTCGACCCGCGCAAGTACTTCTCCAAGACCGTCGAGGCCATG
CGCGACATTTGCATCGCTCGCTACGAAGCCTTCGGCACCGCCGGCAACGCTTCCAAGATCAAGCCGATCTCC
CTGGAAGGCATGTTCCAGCGCTACGCTCGCGGCGAGCTGGATCCGAAGGTCAACTAA
Translation:
MALISMRQMLDHAAEFGYGVPAFNVNNLEQMRAIMEAADKTDSPVIVQASAGARKYAGAPFLRHLILAAIEE
FPHIPVVMHQDHGTSPDVCQRSIQLGFSSVMMDGSLREDGKTPADYDYNVRVTQQTVAFAHACGVSVEGELG
CLGSLETGMAGEEDGVGAEGVLDHSQLLTDPEEAADFVKKTKVDALAIAIGTSHGAYKFTKPPTGDTLSIQR
IKEIHARIPDTHLVMHGSSSVPQDWLAIINEYGGEIKETYGVPVEEIVEGIKYGVRKVNIDTDLRLASTGAI
RRFLAQNPSEFDPRKYFSKTVEAMRDICIARYEAFGTAGNASKIKPISLEGMFQRYARGELDPKVN*
AnnotationID:1872GeneID:1863
AnnotatorUID: diggins
Modification Date/Time: 2005-03-29 07:07:17
Gene Name:fda
Confidence Code:2
GeneProduct:fructose-1,6-bisphosphate aldolase
Cell Localization:()
Synonyms:fba, cbbA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0191,pfam01116
Secondary Functional Category(ies):central intermediary metabolism
EC Number:4.1.2.13
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAA09871.1| (AJ011927) fructose-1,6-bisphosphate aldolase [Pseudomonas
           stutzeri]
          Length = 354

 Score =  685 bits (1768), Expect = 0.0
 Identities = 342/354 (96%), Positives = 346/354 (97%)

>gb|AAN54007.1|AE015538_4 (AE015538) fructose-bisphosphate aldolase, class II, Calvin cycle
           subtype [Shewanella oneidensis MR-1]
          Length = 355

 Score =  567 bits (1462), Expect = e-161
 Identities = 276/354 (77%), Positives = 313/354 (88%)

>gb|AAF42203.1| (AE002537) fructose-bisphosphate aldolase [Neisseria meningitidis
           MC58]
          Length = 354

 Score =  567 bits (1461), Expect = e-161
 Identities = 276/350 (78%), Positives = 308/350 (88%)






 
Structural Features:
COG0191, Fba, Fructose/tagatose bisphosphate aldolase 
[Carbohydrate transport and metabolism]
CD-Length = 286 residues, 100.0% aligned
Score =  363 bits (934), Expect = 1e-101
Identities = 155/330 (46%), Positives = 206/330 (62%), Gaps = 45/330 (13%)

pfam01116, F_bP_aldolase, Fructose-bisphosphate aldolase class-II.
CD-Length = 307 residues, 100.0% aligned
Score =  335 bits (860), Expect = 6e-93
Identities = 140/337 (41%), Positives = 189/337 (56%), Gaps = 39/337 (11%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3729




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20876




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33863




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1863_cdd.html


    		




 
URL:

















    ReferenceID:35284




  
Author/Investigator(s):
        von der Osten CH, Barbas CF 3rd, Wong CH, Sinskey AJ.





  
Title:
        Molecular cloning, nucleotide sequence and fine-structural analysis of the Corynebacterium glutamicum fda gene: structural comparison of C. glutamicum fructose-1,6-biphosphate aldolase to class I and class II aldolases.




  
PubMed:
        2615658


MedLine:
        




    
Source:
Mol Microbiol. 1989 Nov;3(11):1625-37.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2615658

















    ReferenceID:35285




  
Author/Investigator(s):
        Alefounder PR, Perham RN.





  
Title:
        Identification, molecular cloning and sequence analysis of a gene cluster encoding the class II fructose 1,6-bisphosphate aldolase, 3-phosphoglycerate kinase and a putative second glyceraldehyde 3-phosphate dehydrogenase of Escherichia coli.




  
PubMed:
        2546007


MedLine:
        




    
Source:
Mol Microbiol. 1989 Jun;3(6):723-32.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2546007

















    ReferenceID:35286




  
Author/Investigator(s):
        Alefounder PR, Baldwin SA, Perham RN, Short NJ.





  
Title:
        Cloning, sequence analysis and over-expression of the gene for the class II fructose 1,6-bisphosphate aldolase of Escherichia coli.




  
PubMed:
        2649077


MedLine:
        




    
Source:
Biochem J. 1989 Jan 15;257(2):529-34.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2649077









           
Homologs By Global Alignment 

           

       



     
Gene ID:1863
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3266
                           PA0555_tr
                           translation of PA0555
                           354
                           99.99
                           99.99
                           0
                           1825.0
     

     
     
101088
                           gb|AAG03944.1|AE004492_3
                           (AE004492) fructose-1,6-bisphosphate aldolase [Pseudomonas aeruginosa PAO1]
                           354
                           99.99
                           99.99
                           0
                           1825.0
     

     
     
101089
                           emb|CAA09871.1|
                           (AJ011927) fructose-1,6-bisphosphate aldolase [Pseudomonas stutzeri]
                           354
                           96.61
                           97.74
                           0
                           1768.0
     

     
     
101090
                           gb|AAN70527.1|AE016695_1
                           (AE016792) fructose-1,6-bisphosphate aldolase [Pseudomonas putida KT2440]
                           354
                           91.24
                           96.61
                           0
                           1698.0
     

     
     
101091
                           gb|AAO53934.1|
                           (AE016857) fructose-bisphosphate aldolase, class II [Pseudomonas syringae pv. tomato str. DC3000]
                           354
                           90.67
                           96.89
                           0
                           1689.0
     

     






      

          
GC ORFID: 44865How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0555Glimmer Score: 

          
Start: 6424655Stop: 6425719

          
Length: 1065


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA0555 translation of PA0555Homolog Bit Score 707.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6576 (ORF: BLASTX 655.0), t_PA0555 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCACTCATCAGCATGCGCCAGATGCTGGATCACGCCGCCGAATTCGGCTACGGCGTGCCGGCCTTCAAC 
GTCAACAACCTCGAGCAGATGCGCGCCATCATGGAAGCGGCCGACAAGACCGATTCGCCCGTGATCGTCCAG 
GCTTCCGCCGGTGCTCGCAAATACGCCGGCGCGCCCTTCCTGCGCCACCTGATCCTCGCCGCGATCGAAGAA 
TTCCCGCACATCCCGGTGGTCATGCACCAGGATCACGGCACCAGTCCCGACGTCTGCCAGCGTTCCATCCAG 
CTGGGCTTCAGCTCGGTGATGATGGACGGCTCCCTGCGTGAAGACGGCAAGACCCCTGCCGACTACGACTAC 
AACGTCCGCGTGACCCAGCAGACCGTGGCGTTCGCCCATGCCTGCGGCGTGTCCGTGGAAGGCGAGCTGGGC 
TGCCTGGGTTCCCTGGAAACCGGCATGGCCGGCGAGGAAGACGGCGTCGGCGCGGAAGGCGTGCTGGACCAC 
AGCCAGTTGCTGACCGACCCGGAAGAAGCCGCCGACTTCGTGAAGAAGACCAAGGTCGACGCCCTGGCCATC 
GCCATCGGCACCAGCCACGGCGCCTACAAGTTCACCAAGCCGCCGACCGGGGACACCCTGTCGATCCAGCGC 
ATCAAGGAAATCCACGCGCGCATCCCGGACACCCACCTGGTGATGCACGGCTCTTCCTCGGTACCGCAGGAC 
TGGCTGGCGATCATCAACGAGTACGGCGGCGAGATCAAAGAGACCTACGGCGTGCCGGTCGAGGAAATCGTC 
GAGGGCATCAAGTACGGCGTGCGCAAGGTGAACATCGACACCGACCTGCGCCTGGCCTCCACCGGTGCCATC 
CGCCGCTTCCTGGCGCAGAACCCGAGCGAGTTCGACCCGCGCAAGTACTTCTCCAAGACCGTCGAGGCCATG 
CGCGACATTTGCATCGCTCGCTACGAAGCCTTCGGCACCGCCGGCAACGCTTCCAAGATCAAGCCGATCTCC 
CTGGAAGGCATGTTCCAGCGCTACGCTCGCGGCGAGCTGGATCCGAAGGTCAACTAA