ViewGene
Locus Name:
GeneID:
Location:

GeneID:1850
TrivialName: PA14_66550
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66550
PAO1 Ortholog Locus:PA5034
Sequence Length:1068
Protein Length:355
Start: 5937459
Stop: 5936392
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08419.1|AE004916_8 (AE004916) uroporphyrinogen decarboxylase [Pseudomonas aeruginosa PAO1]
Identities = 353/355 (99%), Positives = 353/355 (99%)
Sequence:
ATGACTGCCCTGAAGAACGACCGCTTCCTCCGCGCCCTGCTCAAGCAGCCCGTCGATGTCACCCCCGTCTGG
ATGATGCGCCAGGCCGGTCGTTACCTGCCGGAATATCGTGCCACCCGTGCCAAGGCCGGCGATTTCATGAGC
CTGTGCATGAACCCGGAGCTGGCCTGCGAAGTGACCCTGCAGCCGCTGGACCGTTACCCGCAGCTGGACGCC
GCGATCCTCTTCTCCGACATCCTTACCATTCCCGATGCCATGGGCCAGGGCCTGTATTTCGAGACCGGCGAA
GGCCCGCGCTTCCGCAAGGTGGTCTCGTCGCTGGCCGACATCGAGGCGCTGCCGGTTCCCGATCCGGAACAG
GACCTGGGCTATGTGATGGACGCCGTGCGCACCATCCGCCGCGAGCTGAACGGCCGCGTACCGCTGATCGGC
TTCTCCGGCAGCCCCTGGACCCTCGCCACCTACATGGTCGAGGGCGGCTCGAGCAAGGACTTCCGCAAGTCC
AAGGCGATGCTCTACGACAACCCCAAGGCCATGCACGCTCTGCTGGACAAGCTGGCGCAATCGGTGACCAGC
TACCTCAACGGGCAGATTCGCGCTGGCGCCCAGGCGGTGCAGATCTTCGACTCATGGGGCGGCAGCCTGTCG
GCGGCGGCCTACCAGGAGTTCTCCCTGGTCTACATGCGCAAGATCGTCGATGGATTGATCCGCGAGCACGAC
GGACGCCGGGTGCCGGTGATCCTCTTCACCAAGGGAGGAGGCCTGTGGCTGGAGTCGATGGCCGAGGTCGGC
GCCGAGGCGCTGGGCCTGGACTGGACCTGCGACATCGGCAGCGCCCGGGCCCGTGTCGGTGAGCGCGTAGCC
CTGCAAGGCAACATGGATCCCTCGGTGCTCTACGCCAATCCGGCGGCGATCCGCGCCGAAGTCGCGCGGATC
CTGGCGGCCTACGGCAAGGGTACGGGGCATGTGTTCAATCTCGGCCACGGCATTACCCCGGAAGTCGACCCG
GCCCATGCCGGCGCCTTCTTCGAGGCGGTGCACGAGCTGTCGGCGCAGTACCACGGCTGA
Translation:
MTALKNDRFLRALLKQPVDVTPVWMMRQAGRYLPEYRATRAKAGDFMSLCMNPELACEVTLQPLDRYPQLDA
AILFSDILTIPDAMGQGLYFETGEGPRFRKVVSSLADIEALPVPDPEQDLGYVMDAVRTIRRELNGRVPLIG
FSGSPWTLATYMVEGGSSKDFRKSKAMLYDNPKAMHALLDKLAQSVTSYLNGQIRAGAQAVQIFDSWGGSLS
AAAYQEFSLVYMRKIVDGLIREHDGRRVPVILFTKGGGLWLESMAEVGAEALGLDWTCDIGSARARVGERVA
LQGNMDPSVLYANPAAIRAEVARILAAYGKGTGHVFNLGHGITPEVDPAHAGAFFEAVHELSAQYHG*
AnnotationID:1859GeneID:1850
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 10:10:44
Gene Name:hemE
Confidence Code:2
GeneProduct:uroporphyrinogen decarboxylase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0407,pfam01208
Secondary Functional Category(ies):coenyme metabolism
EC Number:4.1.1.37
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70639.1|AE016707_9 (AE016793) uroporphyrinogen decarboxylase [Pseudomonas putida
           KT2440]
          Length = 354

 Score =  636 bits (1641), Expect = 0.0
 Identities = 312/354 (88%), Positives = 333/354 (94%), Gaps = 1/354 (0%)

>gb|AAO58545.1| (AE016874) uroporphyrinogen decarboxylase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 354

 Score =  635 bits (1638), Expect = 0.0
 Identities = 312/354 (88%), Positives = 333/354 (94%), Gaps = 1/354 (0%)

>gb|AAO70980.1| (AE016845) uroporphyrinogen decarboxylase [Salmonella enterica
           subsp. enterica serovar Typhi Ty2]
          Length = 354

 Score =  548 bits (1411), Expect = e-155
 Identities = 271/354 (76%), Positives = 300/354 (84%), Gaps = 1/354 (0%)








 
Structural Features:
COG0407, HemE, Uroporphyrinogen-III decarboxylase [Coenzyme metabolism]
CD-Length = 352 residues,  99.7% aligned
Score =  466 bits (1200), Expect = 2e-132
Identities = 205/354 (57%), Positives = 248/354 (70%), Gaps = 4/354 (1%)

pfam01208, URO-D, Uroporphyrinogen decarboxylase (URO-D).
CD-Length = 340 residues, 100.0% aligned
Score =  430 bits (1108), Expect = 8e-122
Identities = 187/346 (54%), Positives = 228/346 (65%), Gaps = 6/346 (1%)







   
Genomic Context:






   
Comment:


















    ReferenceID:3703




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20850




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34984




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1850_cdd.html


    		




 
URL:

















    ReferenceID:35859




  
Author/Investigator(s):
        Pido S, Tsoi KW, Umanoff H, Cosloy SD, Russell CS.





  
Title:
        Characterization of a hemA/hemE mutant of E. coli and regulation of hemE.




  
PubMed:
        7849561


MedLine:
        




    
Source:
Cell Mol Biol (Noisy-le-grand). 1994 Nov;40(7):945-56.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=95152340&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:1850
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3252
                           PA5034_tr
                           translation of PA5034
                           355
                           99.43
                           99.43
                           0
                           1830.0
     

     
     
100838
                           gb|AAG08419.1|AE004916_8
                           (AE004916) uroporphyrinogen decarboxylase [Pseudomonas aeruginosa PAO1]
                           355
                           99.43
                           99.43
                           0
                           1830.0
     

     
     
100839
                           gb|AAN70639.1|AE016707_9
                           (AE016793) uroporphyrinogen decarboxylase [Pseudomonas putida KT2440]
                           355
                           87.88
                           93.80
                           1
                           1641.0
     

     
     
100840
                           gb|AAO58545.1|
                           (AE016874) uroporphyrinogen decarboxylase [Pseudomonas syringae pv. tomato str. DC3000]
                           355
                           87.88
                           93.80
                           1
                           1638.0
     

     
     
100841
                           gb|AAB39261.1|
                           (U81261) HemE homolog [Pseudomonas aeruginosa]
                           355
                           82.53
                           82.53
                           60
                           1516.0
     

     






      

          
GC ORFID: 44852How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5034Glimmer Score: 

          
Start: 5201142Stop: 5200075

          
Length: 1068


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5034 translation of PA5034Homolog Bit Score 709.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3301 (ORF: BLASTX 637.0), t_PA5034 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACTGCCCTGAAGAACGACCGCTTCCTCCGCGCCCTGCTCAAGCAGCCCGTCGATGTCACCCCCGTCTGG 
ATGATGCGCCAGGCCGGTCGTTACCTGCCGGAATATCGTGCCACCCGTGCCAAGGCCGGCGATTTCATGAGC 
CTGTGCATGAACCCGGAGCTGGCCTGCGAAGTGACCCTGCAGCCGCTGGACCGTTACCCGCAGCTGGACGCC 
GCGATCCTCTTCTCCGACATCCTTACCATTCCCGATGCCATGGGCCAGGGCCTGTATTTCGAGACCGGCGAA 
GGCCCGCGCTTCCGCAAGGTGGTCTCGTCGCTGGCCGACATCGAGGCGCTGCCGGTTCCCGATCCGGAACAG 
GACCTGGGCTATGTGATGGACGCCGTGCGCACCATCCGCCGCGAGCTGAACGGCCGCGTACCGCTGATCGGC 
TTCTCCGGCAGCCCCTGGACCCTCGCCACCTACATGGTCGAGGGCGGCTCGAGCAAGGACTTCCGCAAGTCC 
AAGGCGATGCTCTACGACAACCCCAAGGCCATGCACGCTCTGCTGGACAAGCTGGCGCAATCGGTGACCAGC 
TACCTCAACGGGCAGATTCGCGCTGGCGCCCAGGCGGTGCAGATCTTCGACTCATGGGGCGGCAGCCTGTCG 
GCGGCGGCCTACCAGGAGTTCTCCCTGGTCTACATGCGCAAGATCGTCGATGGATTGATCCGCGAGCACGAC 
GGACGCCGGGTGCCGGTGATCCTCTTCACCAAGGGAGGAGGCCTGTGGCTGGAGTCGATGGCCGAGGTCGGC 
GCCGAGGCGCTGGGCCTGGACTGGACCTGCGACATCGGCAGCGCCCGGGCCCGTGTCGGTGAGCGCGTAGCC 
CTGCAAGGCAACATGGATCCCTCGGTGCTCTACGCCAATCCGGCGGCGATCCGCGCCGAAGTCGCGCGGATC 
CTGGCGGCCTACGGCAAGGGTACGGGGCATGTGTTCAATCTCGGCCACGGCATTACCCCGGAAGTCGACCCG 
GCCCATGCCGGCGCCTTCTTCGAGGCGGTGCACGAGCTGTCGGCGCAGTACCACGGCTGA