ViewGene
Locus Name:
GeneID:
Location:

GeneID:1847
TrivialName: PA14_45580
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_45580
PAO1 Ortholog Locus:PA1459
Sequence Length:1107
Protein Length:368
Start: 4054876
Stop: 4053770
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: dbj|BAA33550.1| (AB012767) CheB [Pseudomonas aeruginosa]
Identities = 364/368 (98%), Positives = 366/368 (99%)
Sequence:
ATGGCAGTCAAAGTCCTGGTGGTGGACGATTCGGGGTTCTTCCGCCGCCGTGTCTCGGAGATTCTCTCCGCC
GATGGACAGATCCAGGTGGTCGGCACCGCCACCAATGGCCGCGAGGCCATCGAGCAGGCGCTGGCCCTGAGA
CCCGATGTGATCACCATGGATTACGAGATGCCGCTGATGGACGGCATCACCGCGGTACGCAACATCATGCAG
CGTTGCCCGACCCCGGTCCTGATGTTCTCCTCGCTGACCCACGAGGGCGCGCGGGTGACCCTGGATGCGCTG
GACGCCGGAGCGGTCGACTACCTGCCGAAAAATTTCGAGGACATCTCGCGCAACCCGGACAAGGTCCGCCAG
TTGCTTTGCGAGAAGGTCCTGACCATCGCCCGTAGCAATCGTCGCTCGATCAGCCTGCCGCCGCTGCCTTCG
GCCACCTCGTCCAGCCATGCGCCGGCGTCGTCCTCCAGCCTCGGCGCGAGCGCGCGTGCCAGTGCCGGCGCG
TCGCCGGCCCCGGCGTCGACCTCGGCGGCGCCGAAGCGCAAGGCCTACCGGCTGGTCGCGATCGGTACCTCC
ACCGGTGGTCCGGTTGCCTTGCAGCGGGTGCTGACCCAGTTGCCGGCCAACTTCCCGGCGCCGCTGGTGCTG
ATCCAGCACATGCCGGCGGCCTTCACCAAGGCTTTCGCCGAGCGCCTCGACAAGCTGTGCCGGATCAGCGTC
AAGGAAGCAGAGGACGGCGACATCCTGCGTCCCGGCCTGGCGCTGCTGGCGCCGGGCGGCAAGCAGATGATG
GTCGACGGCCGCGGCACGGTGCGCATCCTCCCCGGCGACGAACGCCTGAACTACAAGCCCTGCGTCGACGTG
ACCTTCGGCTCGGCGGCCAAGGCCTACAACGACAAGGTTCTGGCGGTGGTCCTCACCGGCATGGGCGCCGAC
GGACGCGAGGGCGCGCGCCTGCTCAAGCAGGGCGGCAGCCAGGTGTGGGCGCAGGACGAGGCCAGTTGCGTG
ATCTACGGGATGCCGATGGCCGTGGTCAAGGCCAACCTGGCGGACGCGGTGTACGGGCTCGACGACATCGGC
CGGCACCTGGTCGAGGCGTGCCAGTAA
Translation:
MAVKVLVVDDSGFFRRRVSEILSADGQIQVVGTATNGREAIEQALALRPDVITMDYEMPLMDGITAVRNIMQ
RCPTPVLMFSSLTHEGARVTLDALDAGAVDYLPKNFEDISRNPDKVRQLLCEKVLTIARSNRRSISLPPLPS
ATSSSHAPASSSSLGASARASAGASPAPASTSAAPKRKAYRLVAIGTSTGGPVALQRVLTQLPANFPAPLVL
IQHMPAAFTKAFAERLDKLCRISVKEAEDGDILRPGLALLAPGGKQMMVDGRGTVRILPGDERLNYKPCVDV
TFGSAAKAYNDKVLAVVLTGMGADGREGARLLKQGGSQVWAQDEASCVIYGMPMAVVKANLADAVYGLDDIG
RHLVEACQ*
AnnotationID:1856GeneID:1847
AnnotatorUID: diggins
Modification Date/Time: 2005-02-24 13:01:39
Gene Name:
Confidence Code:3
GeneProduct:putative chemotaxis methylesterase
Cell Localization:()
Synonyms:cheB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(10) Chemotaxis
Alternate Gene Product Name:putative protein-glutamate methylesterase CheB
Functional Category Confidence Code:5
COGs:COG2201,pfam01339
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69916.1|AE016630_7 (AE016790) protein-glutamate methylesterase CheB [Pseudomonas
           putida KT2440]
          Length = 370

 Score =  608 bits (1569), Expect = e-173
 Identities = 315/371 (84%), Positives = 340/371 (91%), Gaps = 6/371 (1%)

>gb|AAO55501.1| (AE016862) protein-glutamate methylesterase CheB [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 390

 Score =  590 bits (1521), Expect = e-168
 Identities = 309/389 (79%), Positives = 336/389 (86%), Gaps = 22/389 (5%)

>gb|AAN56205.1|AE015758_9 (AE015758) protein-glutamate methylesterase CheB [Shewanella
           oneidensis MR-1]
          Length = 374

 Score =  417 bits (1072), Expect = e-116
 Identities = 214/371 (57%), Positives = 272/371 (73%), Gaps = 8/371 (2%)








 
Structural Features:
COG2201, CheB, Chemotaxis response regulator containing a CheY-like receiver 
domain and a methylesterase domain 
[Cell motility and secretion / Signal transduction mechanisms]
CD-Length = 350 residues,  98.0% aligned
Score =  386 bits (994), Expect = 1e-108
Identities = 171/369 (46%), Positives = 229/369 (62%), Gaps = 29/369 (7%)

pfam01339, CheB methylesterase.
CD-Length = 184 residues,  99.5% aligned
Score =  188 bits (478), Expect = 1e-48
Identities = 85/183 (46%), Positives = 118/183 (64%), Gaps = 3/183 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3697




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20844




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37355




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1847_cdd.html


    		




 
URL:

















    ReferenceID:37958




  
Author/Investigator(s):
        Kato J, Nakamura T, Kuroda A, Ohtake H.





  
Title:
        http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10052136




  
PubMed:
        10052136


MedLine:
        




    
Source:
Biosci Biotechnol Biochem. 1999 Jan;63(1):155-61.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10052136









           
Homologs By Global Alignment 

           

       



     
Gene ID:1847
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
100242
                           dbj|BAA33550.1|
                           (AB012767) CheB [Pseudomonas aeruginosa]
                           368
                           98.91
                           99.45
                           0
                           1836.0
     

     
     
3238
                           PA1459_tr
                           translation of PA1459
                           368
                           98.64
                           99.18
                           0
                           1832.0
     

     
     
100243
                           gb|AAG04848.1|AE004575_7
                           (AE004575) probable methyltransferase [Pseudomonas aeruginosa PAO1]
                           368
                           98.64
                           99.18
                           0
                           1832.0
     

     
     
100245
                           gb|AAN69916.1|AE016630_7
                           (AE016790) protein-glutamate methylesterase CheB [Pseudomonas putida KT2440]
                           372
                           84.67
                           91.39
                           6
                           1574.0
     

     
     
100244
                           gb|AAC08065.1|
                           (AF031898) CheB [Pseudomonas putida]
                           378
                           84.65
                           91.26
                           14
                           1587.0
     

     






      

          
GC ORFID: 44849How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1459Glimmer Score: 

          
Start: 899033Stop: 900139

          
Length: 1107


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA1459 translation of PA1459Homolog Bit Score 710.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7943 (ORF: BLASTX 592.0), t_PA0173 (ORF: BLASTX 241.0), psyr_15may02_Scaffold15_revised_gene2551 (ORF: BLASTX 233.0), t_PA1459 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCAGTCAAAGTCCTGGTGGTGGACGATTCGGGGTTCTTCCGCCGCCGTGTCTCGGAGATTCTCTCCGCC 
GATGGACAGATCCAGGTGGTCGGCACCGCCACCAATGGCCGCGAGGCCATCGAGCAGGCGCTGGCCCTGAGA 
CCCGATGTGATCACCATGGATTACGAGATGCCGCTGATGGACGGCATCACCGCGGTACGCAACATCATGCAG 
CGTTGCCCGACCCCGGTCCTGATGTTCTCCTCGCTGACCCACGAGGGCGCGCGGGTGACCCTGGATGCGCTG 
GACGCCGGAGCGGTCGACTACCTGCCGAAAAATTTCGAGGACATCTCGCGCAACCCGGACAAGGTCCGCCAG 
TTGCTTTGCGAGAAGGTCCTGACCATCGCCCGTAGCAATCGTCGCTCGATCAGCCTGCCGCCGCTGCCTTCG 
GCCACCTCGTCCAGCCATGCGCCGGCGTCGTCCTCCAGCCTCGGCGCGAGCGCGCGTGCCAGTGCCGGCGCG 
TCGCCGGCCCCGGCGTCGACCTCGGCGGCGCCGAAGCGCAAGGCCTACCGGCTGGTCGCGATCGGTACCTCC 
ACCGGTGGTCCGGTTGCCTTGCAGCGGGTGCTGACCCAGTTGCCGGCCAACTTCCCGGCGCCGCTGGTGCTG 
ATCCAGCACATGCCGGCGGCCTTCACCAAGGCTTTCGCCGAGCGCCTCGACAAGCTGTGCCGGATCAGCGTC 
AAGGAAGCAGAGGACGGCGACATCCTGCGTCCCGGCCTGGCGCTGCTGGCGCCGGGCGGCAAGCAGATGATG 
GTCGACGGCCGCGGCACGGTGCGCATCCTCCCCGGCGACGAACGCCTGAACTACAAGCCCTGCGTCGACGTG 
ACCTTCGGCTCGGCGGCCAAGGCCTACAACGACAAGGTTCTGGCGGTGGTCCTCACCGGCATGGGCGCCGAC 
GGACGCGAGGGCGCGCGCCTGCTCAAGCAGGGCGGCAGCCAGGTGTGGGCGCAGGACGAGGCCAGTTGCGTG 
ATCTACGGGATGCCGATGGCCGTGGTCAAGGCCAACCTGGCGGACGCGGTGTACGGGCTCGACGACATCGGC 
CGGCACCTGGTCGAGGCGTGCCAGTAA