ViewGene
Locus Name:
GeneID:
Location:

GeneID:1844
TrivialName: PA14_57380
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57380
PAO1 Ortholog Locus:PA4415
Sequence Length:1083
Protein Length:360
Start: 5113330
Stop: 5112248
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07803.1|AE004856_14 (AE004856) phospho-N-acetylmuramoyl-pentapeptide-transferase [Pseudomonas aeruginosa PAO1]
Identities = 360/360 (100%), Positives = 360/360 (100%)
Sequence:
ATGCTCCTGCTGCTGGCCGAATACCTGCAACAGTTCTACAAGGGCTTCGGCGTCTTCCAGTACCTGACCCTG
CGCGGCATTCTCAGCGTGCTCACCGCGCTGTCGCTGTCGCTGTGGCTGGGGCCCTGGATGATCCGTACCTTG
CAGATCCGCCAGATCGGCCAGGCCGTGCGCAACGACGGTCCGCAGTCGCACCTGTCGAAGAAGGGCACCCCG
ACCATGGGCGGCGCCCTGATCCTTACCGCCATCGCCATCAGCACGCTGCTGTGGGCGGATCTTTCCAACCGC
TACGTGTGGGTGGTGCTGGTCGTTACCCTGCTGTTCGGTGCCATCGGCTGGGTGGACGACTACCGCAAGGTG
ATCGAGAAGAACTCCCGCGGCCTGCCGAGCCGCTGGAAGTACTTCTGGCAGTCGGTGTTTGGCATCGGCGCC
GCCGTGTTCCTCTATATGACTGCCGAAACCCCGATCGAGACCACCCTGATCGTGCCGATGCTGAAGAGCGTC
GAGATCCAGTTGGGCATCTTCTTCGTGGTCCTGACCTACTTCGTCATCGTCGGTTCGAGCAATGCGGTGAAC
CTCACCGACGGTCTCGACGGCCTGGCGATCATGCCGACGGTAATGGTTGCCGGCGCGCTGGGCATCTTCTGC
TACCTGTCGGGCAACGTGAAGTTCGCCGAGTACCTGCTGATTCCCAACGTACCGGGCGCCGGCGAGCTGATC
GTGTTCTGCGCCGCGCTGGTCGGCGCCGGCCTCGGCTTCCTCTGGTTCAACACCTATCCGGCGCAGGTCTTC
ATGGGCGACGTCGGCGCGCTGGCGCTGGGCGCCGCGCTGGGCACCATCGCGGTGATCGTGCGCCAGGAGATC
GTGCTGTTCATCATGGGTGGGGTGTTCGTCATGGAAACCCTCTCGGTGATGATCCAGGTCGCTTCCTTCAAG
CTGACCGGACGCCGCGTCTTCCGCATGGCGCCGATCCATCACCATTTCGAACTGAAAGGCTGGCCGGAGCCG
CGCGTGATCGTGCGCTTCTGGATCATCACCGTGATCCTGGTGCTGATCGGCCTCGCCACCTTGAAGCTGCGT
TGA
Translation:
MLLLLAEYLQQFYKGFGVFQYLTLRGILSVLTALSLSLWLGPWMIRTLQIRQIGQAVRNDGPQSHLSKKGTP
TMGGALILTAIAISTLLWADLSNRYVWVVLVVTLLFGAIGWVDDYRKVIEKNSRGLPSRWKYFWQSVFGIGA
AVFLYMTAETPIETTLIVPMLKSVEIQLGIFFVVLTYFVIVGSSNAVNLTDGLDGLAIMPTVMVAGALGIFC
YLSGNVKFAEYLLIPNVPGAGELIVFCAALVGAGLGFLWFNTYPAQVFMGDVGALALGAALGTIAVIVRQEI
VLFIMGGVFVMETLSVMIQVASFKLTGRRVFRMAPIHHHFELKGWPEPRVIVRFWIITVILVLIGLATLKLR
*
AnnotationID:1853GeneID:1844
AnnotatorUID: diggins
Modification Date/Time: 2005-05-02 18:06:19
Gene Name:mraY
Confidence Code:2
GeneProduct:UDP-N-acetylmuramyl pentapeptide phosphotransferas
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:phospho-N-acetylmuramoyl-pentapeptide-transferase
Functional Category Confidence Code:5
COGs:COG0472
Secondary Functional Category(ies):
EC Number:2.7.8.13
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57860.1| (AE016871) phospho-N-acetylmuramoyl-pentapeptide-transferase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 360

 Score =  647 bits (1668), Expect = 0.0
 Identities = 325/360 (90%), Positives = 341/360 (94%)


>gb|AAM35665.1| (AE011708) phospho-N-acetylmuramoyl-pentapeptide-transferase
           [Xanthomonas axonopodis pv. citri str. 306]
          Length = 361

 Score =  518 bits (1334), Expect = e-146
 Identities = 255/361 (70%), Positives = 297/361 (82%), Gaps = 1/361 (0%)






 
Structural Features:
COG0472, Rfe, UDP-N-acetylmuramyl pentapeptide 
phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 319 residues,  97.5% aligned
Score =  226 bits (578), Expect = 6e-60
Identities = 147/340 (43%), Positives = 204/340 (60%), Gaps = 35/340 (10%)







   
Genomic Context:






   
Comment:


















    ReferenceID:3691




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20838




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:29640




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1844_cdd.html


    		




 
URL:

















    ReferenceID:32801




  
Author/Investigator(s):
        Azzolina BA, Yuan X, Anderson MS, El-Sherbeini M.





  
Title:
        The cell wall and cell division gene cluster in the Mra operon of Pseudomonas aeruginosa: cloning, production, and purification of active enzymes.




  
PubMed:
        11281713


MedLine:
        




    
Source:
Protein Expr Purif. 2001 Apr;21(3):393-400.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11281713









           
Homologs By Global Alignment 

           

       



     
Gene ID:1844
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3232
                           PA4415_tr
                           translation of PA4415
                           360
                           99.99
                           99.99
                           0
                           1834.0
     

     
     
100068
                           gb|AAG07803.1|AE004856_14
                           (AE004856) phospho-N-acetylmuramoyl-pentapeptide-transferase [Pseudomonas aeruginosa PAO1]
                           360
                           99.99
                           99.99
                           0
                           1834.0
     

     
     
100069
                           gb|AAG22121.1|
                           (AY008276) MraY [Pseudomonas aeruginosa]
                           360
                           99.44
                           99.72
                           0
                           1824.0
     

     
     
100070
                           gb|AAN66957.1|AE016324_7
                           (AE016779) phospho-N-acetylmuramoyl-pentapeptide-transferase [Pseudomonas putida KT2440]
                           360
                           90.83
                           95.55
                           0
                           1699.0
     

     
     
100071
                           gb|AAO57860.1|
                           (AE016871) phospho-N-acetylmuramoyl-pentapeptide-transferase [Pseudomonas syringae pv. tomato str. DC3000]
                           360
                           90.27
                           94.72
                           0
                           1668.0
     

     






      

          
GC ORFID: 44846How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4415Glimmer Score: 

          
Start: 4377008Stop: 4375926

          
Length: 1083


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4415 translation of PA4415Homolog Bit Score 711.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1125 (ORF: BLASTX 647.0), t_PA4415 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTCCTGCTGCTGGCCGAATACCTGCAACAGTTCTACAAGGGCTTCGGCGTCTTCCAGTACCTGACCCTG 
CGCGGCATTCTCAGCGTGCTCACCGCGCTGTCGCTGTCGCTGTGGCTGGGGCCCTGGATGATCCGTACCTTG 
CAGATCCGCCAGATCGGCCAGGCCGTGCGCAACGACGGTCCGCAGTCGCACCTGTCGAAGAAGGGCACCCCG 
ACCATGGGCGGCGCCCTGATCCTTACCGCCATCGCCATCAGCACGCTGCTGTGGGCGGATCTTTCCAACCGC 
TACGTGTGGGTGGTGCTGGTCGTTACCCTGCTGTTCGGTGCCATCGGCTGGGTGGACGACTACCGCAAGGTG 
ATCGAGAAGAACTCCCGCGGCCTGCCGAGCCGCTGGAAGTACTTCTGGCAGTCGGTGTTTGGCATCGGCGCC 
GCCGTGTTCCTCTATATGACTGCCGAAACCCCGATCGAGACCACCCTGATCGTGCCGATGCTGAAGAGCGTC 
GAGATCCAGTTGGGCATCTTCTTCGTGGTCCTGACCTACTTCGTCATCGTCGGTTCGAGCAATGCGGTGAAC 
CTCACCGACGGTCTCGACGGCCTGGCGATCATGCCGACGGTAATGGTTGCCGGCGCGCTGGGCATCTTCTGC 
TACCTGTCGGGCAACGTGAAGTTCGCCGAGTACCTGCTGATTCCCAACGTACCGGGCGCCGGCGAGCTGATC 
GTGTTCTGCGCCGCGCTGGTCGGCGCCGGCCTCGGCTTCCTCTGGTTCAACACCTATCCGGCGCAGGTCTTC 
ATGGGCGACGTCGGCGCGCTGGCGCTGGGCGCCGCGCTGGGCACCATCGCGGTGATCGTGCGCCAGGAGATC 
GTGCTGTTCATCATGGGTGGGGTGTTCGTCATGGAAACCCTCTCGGTGATGATCCAGGTCGCTTCCTTCAAG 
CTGACCGGACGCCGCGTCTTCCGCATGGCGCCGATCCATCACCATTTCGAACTGAAAGGCTGGCCGGAGCCG 
CGCGTGATCGTGCGCTTCTGGATCATCACCGTGATCCTGGTGCTGATCGGCCTCGCCACCTTGAAGCTGCGT 
TGA