ViewGene
Locus Name:
GeneID:
Location:

GeneID:1839
TrivialName: PA14_41920
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_41920
PAO1 Ortholog Locus:PA1750
Sequence Length:1077
Protein Length:358
Start: 3742858
Stop: 3743934
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05139.1|AE004601_4 (AE004601) phospho-2-dehydro-3-deoxyheptonate aldolase [Pseudomonas aeruginosa PAO1]
Identities = 357/358 (99%), Positives = 357/358 (99%)
Sequence:
ATGGCTGATTTACAGATCGACGACCTTAACGTTGCCTCCAACGAGACCCTGATCACGCCGGAGCAGCTCAAG
CGTGAAATTCCCCTGACCGACAAGGCCCTGCAGACCGTGGCCCATGGTCGCCAGGTGGTGCGCGACATCCTG
GATGGCAAGGACCACCGTCTGTTCGTGGTGATCGGCCCCTGCTCCATCCACGACATCAAGGCCGCCCACGAA
TACGCCGACCGCCTCAAGGCGCTCGCGGCCGAAGTGGCGGATACGCTGTTCCTGGTGATGCGCGTGTACTTC
GAGAAGCCGCGTACCACGGTGGGCTGGAAAGGCCTGATCAACGATCCGTACCTGGACGACTCGTTCAAGATC
CAGGATGGCCTGCACATCGGTCGCCAACTGCTCCGCGACCTCGCCGAGAAAGGCTTGCCCACCGCCACCGAA
GCGCTCGACCCGATTTCCCCGCAGTACCTGCAGGACCTGATCAGCTGGTCGGCGATCGGCGCCCGTACCACC
GAATCGCAGACCCACCGCGAGATGGCCTCCGGCCTGTCTTCCGCGGTCGGCTTCAAGAACGGCACCGATGGC
AGCCTGACCGTGGCGATCAATGCCCTGCAGTCGGTCTCCAGCCCGCATCGCTTCCTCGGCATCAACCAGCAG
GGCGGCGTATCCATCGTCACCACCAAGGGCAACCGCTACGGTCACGTGGTGTTGCGCGGCGGCAACGGCAAG
CCGAACTACGATTCGGTCAGCGTCGCGCTCTGCGAGCAGGACCTGAACAAGGCGAAAATCCCGCTGAACATC
ATGGTCGACTGCAGCCACGCCAACTCCAACAAGGACCCGGCCCTGCAACCGCTGGTGATGGACAACGTCAGC
AACCAGATCGTCGAAGGCAACAACTCGATCGTCGGCCTGATGGTGGAAAGCCACCTGGGCTGGGGCAGCCAG
CCGATTCCGAAGGATCTCGACCAACTTCAGTACGGCGTCTCCATCACCGACGCCTGCATCGACTGGGCGACC
ACCGAGAAGAGCATCCGCAGCATGCACGCCAAGCTCAAGGACGTGCTGCCGAAACGCCAGCGCGGCTGA
Translation:
MADLQIDDLNVASNETLITPEQLKREIPLTDKALQTVAHGRQVVRDILDGKDHRLFVVIGPCSIHDIKAAHE
YADRLKALAAEVADTLFLVMRVYFEKPRTTVGWKGLINDPYLDDSFKIQDGLHIGRQLLRDLAEKGLPTATE
ALDPISPQYLQDLISWSAIGARTTESQTHREMASGLSSAVGFKNGTDGSLTVAINALQSVSSPHRFLGINQQ
GGVSIVTTKGNRYGHVVLRGGNGKPNYDSVSVALCEQDLNKAKIPLNIMVDCSHANSNKDPALQPLVMDNVS
NQIVEGNNSIVGLMVESHLGWGSQPIPKDLDQLQYGVSITDACIDWATTEKSIRSMHAKLKDVLPKRQRG*
AnnotationID:1848GeneID:1839
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 09:09:21
Gene Name:aroF-1
Confidence Code:2
GeneProduct:phospho-2-dehydro-3-deoxyheptonate aldolase
Cell Localization:()
Synonyms:aroF
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0722,pfam00793
Secondary Functional Category(ies):
EC Number:4.1.2.15
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67937.1|AE016426_2 (AE016782) phospho-2-dehydro-3-deoxyheptonate aldolase, class I
           [Pseudomonas putida KT2440]
          Length = 370

 Score =  642 bits (1656), Expect = 0.0
 Identities = 317/358 (88%), Positives = 338/358 (94%)

>gb|AAO55789.1| (AE016864) phospho-2-dehydro-3-deoxyheptonate aldolase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 358

 Score =  632 bits (1630), Expect = 0.0
 Identities = 313/357 (87%), Positives = 334/357 (93%)

>gb|AAN81572.1|AE016764_254 (AE016764) Phospho-2-dehydro-3-deoxyheptonate aldolase,
           Tyr-sensitive [Escherichia coli CFT073]
          Length = 379

 Score =  405 bits (1040), Expect = e-112
 Identities = 200/350 (57%), Positives = 258/350 (73%), Gaps = 1/350 (0%)








 
Structural Features:
COG0722, AroG, 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase 
[Amino acid transport and metabolism]
CD-Length = 351 residues,  98.9% aligned
Score =  544 bits (1403), Expect = 7e-156
Identities = 206/348 (59%), Positives = 248/348 (71%), Gaps = 1/348 (0%)

pfam00793, DAHP_synth_1, DAHP synthetase I family.
CD-Length = 271 residues,  95.6% aligned
Score =  258 bits (660), Expect = 8e-70
Identities = 131/294 (44%), Positives = 157/294 (53%), Gaps = 36/294 (12%)







   
Genomic Context:






   
Comment:


















    ReferenceID:3681




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20828




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37125




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1839_cdd.html


    		




 
URL:

















    ReferenceID:37834




  
Author/Investigator(s):
        Xie G, Bonner CA, Jensen RA.





  
Title:
        A probable mixed-function supraoperon in Pseudomonas exhibits gene organization features of both intergenomic conservation and gene shuffling.




  
PubMed:
        10368439


MedLine:
        




    
Source:
J Mol Evol. 1999 Jul;49(1):108-21.

Erratum in:
    * J Mol Evol 2000 Feb;50(2):202.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10368439

















    ReferenceID:37835




  
Author/Investigator(s):
        Hudson GS, Davidson BE.





  
Title:
        Nucleotide sequence and transcription of the phenylalanine and tyrosine operons of Escherichia coli K12.




  
PubMed:
        6396419


MedLine:
        




    
Source:
J Mol Biol. 1984 Dec 25;180(4):1023-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6396419

















    ReferenceID:37836




  
Author/Investigator(s):
        Shultz J, Hermodson MA, Garner CC, Herrmann KM.





  
Title:
        The nucleotide sequence of the aroF gene of Escherichia coli and the amino acid sequence of the encoded protein, the tyrosine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase.




  
PubMed:
        6146618


MedLine:
        




    
Source:
J Biol Chem. 1984 Aug 10;259(15):9655-61.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6146618









           
Homologs By Global Alignment 

           

       



     
Gene ID:1839
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3225
                           PA1750_tr
                           translation of PA1750
                           358
                           99.72
                           99.72
                           0
                           1837.0
     

     
     
99873
                           gb|AAG05139.1|AE004601_4
                           (AE004601) phospho-2-dehydro-3-deoxyheptonate aldolase [Pseudomonas aeruginosa PAO1]
                           358
                           99.72
                           99.72
                           0
                           1837.0
     

     
     
99875
                           gb|AAO55789.1|
                           (AE016864) phospho-2-dehydro-3-deoxyheptonate aldolase [Pseudomonas syringae pv. tomato str. DC3000]
                           358
                           87.43
                           93.29
                           0
                           1628.0
     

     
     
99874
                           gb|AAN67937.1|AE016426_2
                           (AE016782) phospho-2-dehydro-3-deoxyheptonate aldolase, class I [Pseudomonas putida KT2440]
                           370
                           85.67
                           91.35
                           12
                           1656.0
     

     
     
99877
                           gb|AAF93860.1|
                           (AE004155) phospho-2-dehydro-3-deoxyheptonate aldolase, tyr-sensitive [Vibrio cholerae O1 biovar eltor str. N16961]
                           358
                           60.89
                           78.21
                           1
                           1150.0
     

     






      

          
GC ORFID: 44841How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1750Glimmer Score: 

          
Start: 1211051Stop: 1209975

          
Length: 1077


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1750 translation of PA1750Homolog Bit Score 712.0

          
Other Homologs:  psyr_15may02_Scaffold13_revised_gene2184 (ORF: BLASTX 589.0), t_PA1750 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCTGATTTACAGATCGACGACCTTAACGTTGCCTCCAACGAGACCCTGATCACGCCGGAGCAGCTCAAG 
CGTGAAATTCCCCTGACCGACAAGGCCCTGCAGACCGTGGCCCATGGTCGCCAGGTGGTGCGCGACATCCTG 
GATGGCAAGGACCACCGTCTGTTCGTGGTGATCGGCCCCTGCTCCATCCACGACATCAAGGCCGCCCACGAA 
TACGCCGACCGCCTCAAGGCGCTCGCGGCCGAAGTGGCGGATACGCTGTTCCTGGTGATGCGCGTGTACTTC 
GAGAAGCCGCGTACCACGGTGGGCTGGAAAGGCCTGATCAACGATCCGTACCTGGACGACTCGTTCAAGATC 
CAGGATGGCCTGCACATCGGTCGCCAACTGCTCCGCGACCTCGCCGAGAAAGGCTTGCCCACCGCCACCGAA 
GCGCTCGACCCGATTTCCCCGCAGTACCTGCAGGACCTGATCAGCTGGTCGGCGATCGGCGCCCGTACCACC 
GAATCGCAGACCCACCGCGAGATGGCCTCCGGCCTGTCTTCCGCGGTCGGCTTCAAGAACGGCACCGATGGC 
AGCCTGACCGTGGCGATCAATGCCCTGCAGTCGGTCTCCAGCCCGCATCGCTTCCTCGGCATCAACCAGCAG 
GGCGGCGTATCCATCGTCACCACCAAGGGCAACCGCTACGGTCACGTGGTGTTGCGCGGCGGCAACGGCAAG 
CCGAACTACGATTCGGTCAGCGTCGCGCTCTGCGAGCAGGACCTGAACAAGGCGAAAATCCCGCTGAACATC 
ATGGTCGACTGCAGCCACGCCAACTCCAACAAGGACCCGGCCCTGCAACCGCTGGTGATGGACAACGTCAGC 
AACCAGATCGTCGAAGGCAACAACTCGATCGTCGGCCTGATGGTGGAAAGCCACCTGGGCTGGGGCAGCCAG 
CCGATTCCGAAGGATCTCGACCAACTTCAGTACGGCGTCTCCATCACCGACGCCTGCATCGACTGGGCGACC 
ACCGAGAAGAGCATCCGCAGCATGCACGCCAAGCTCAAGGACGTGCTGCCGAAACGCCAGCGCGGCTGA